Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 15595051

GI number: 15595051

Start: 741396

End: 742628

Strand: Reverse

Name: cca [H]

Synonym: BB0706

Alternate gene names: 15595051

Gene position: 742628-741396 (Counterclockwise)

Preceding gene: 15595068

Following gene: 15595034

Centisome position: 81.54

GC content: 22.38

Gene sequence:

>1233_bases
ATGAATCTAGGGAAAAACAATCCAAATATAATTAAAATTGGTAAAATATTTAAAAAAAATAACTACGAATTTTATTTAGT
TGGAGGCGCTTTAAGAGACTTACTGCTTAATAAACAGCCTTACGATTTTGATTTTGCAACAAATGCAACTCCTGAAGAAA
TAATAACATTATTTCCAAATAACATCAAAACAGGAATAAAACATGGCACAATTGGTATTATTTTTAATAAAAAAATCTTT
GAAATCACCACATACAGAATAGAAAAAGAATATGAAAACAACAGAGCCCCCAAACAAGTAGAATATACTAAAAATTTACT
TAAAGATCTTGAAAGAAGAGATTTTACAATTAATGCAATTGCAATGGATATTTTCAACTTCAACATAATAGATTGCTATA
ATGGGAAAAAAGACCTTAATAAGAAAATAATAAGATGCATAGGAAATCCAAACAAAAGACTTGAAGAAGACGCCCTTAGA
ATACTTAGAGCAGCAAGATTTTCATCCACACTTAATTTTAACATTGAAAAAAATACTTTAATTTCAATGAAATATAAAAA
AGAAAATATTTTAATGATTTCAAAAGAAAGAATAAAAAATGAATTTCACAAATTGTTAGAAGGCATAAATATACAAAAAG
GAATTTATTATCTTAAAAAAGTTGATTTTTTTAAAAATTTTTTTAATCTAGAAATAAAAACAAAAGTAATCAAAAAAATT
GCTCTACTTGATAAAAACAAATTTTATCTAAAGGCAATCACAATATTGACAATTAAAAAACCTATAAAAGAACTAAAAGA
AAAATTAACTTTACTTAAATTCTCAAATAAAGAAATTAAGCTGATTTTATTTTATAGAGGCATAATCGATAATAACAATA
TTTTTAATGTCAAAAAATTAAGTGATATTAGATATTTGCTTAGCAAAAGCACAAGAGAACATTATAAAGAAATAATTGAT
ATATACAAAGCACTCAAAGGAAAAAATAAAAGATATTTATTTATAATAAAAAACATAAAAAGAAAAAAATTGCTAAAAAA
TCCTCTCTCTTTAAAAGATTTAAAAATAAACGGAAAAGATATTCAAAATCTAGAACAAATAGAAAACAAAAATATAGGTA
AAATTTTAAATATGCTACTAAGATGTGTAATTGAAAATCCCAAGCTTAATACTAAAAATTATCTTATAAAAAAAATCAAA
ACCTTAAAGGTTAATGTTTTCCATAGCTTTTAA

Upstream 100 bases:

>100_bases
CCATTTAAAAAATAAAGTACTACTTACAACATACTTCAAATAAATTAAAATTGTAAATCAATTTTAGAAAAAAATTATTT
ACACTATAATATAATTTTAT

Downstream 100 bases:

>100_bases
AGCTACTTCAGCCGCTCTCATTTCGGCTTCTTTTTTAGATTTGCCCTTTCCATTTGATATAAAATTTTCTCCAACATAAA
GTTCCACACAAAAAACTTTA

Product: polynucleotide adenylyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNLGKNNPNIIKIGKIFKKNNYEFYLVGGALRDLLLNKQPYDFDFATNATPEEIITLFPNNIKTGIKHGTIGIIFNKKIF
EITTYRIEKEYENNRAPKQVEYTKNLLKDLERRDFTINAIAMDIFNFNIIDCYNGKKDLNKKIIRCIGNPNKRLEEDALR
ILRAARFSSTLNFNIEKNTLISMKYKKENILMISKERIKNEFHKLLEGINIQKGIYYLKKVDFFKNFFNLEIKTKVIKKI
ALLDKNKFYLKAITILTIKKPIKELKEKLTLLKFSNKEIKLILFYRGIIDNNNIFNVKKLSDIRYLLSKSTREHYKEIID
IYKALKGKNKRYLFIIKNIKRKKLLKNPLSLKDLKINGKDIQNLEQIENKNIGKILNMLLRCVIENPKLNTKNYLIKKIK
TLKVNVFHSF

Sequences:

>Translated_410_residues
MNLGKNNPNIIKIGKIFKKNNYEFYLVGGALRDLLLNKQPYDFDFATNATPEEIITLFPNNIKTGIKHGTIGIIFNKKIF
EITTYRIEKEYENNRAPKQVEYTKNLLKDLERRDFTINAIAMDIFNFNIIDCYNGKKDLNKKIIRCIGNPNKRLEEDALR
ILRAARFSSTLNFNIEKNTLISMKYKKENILMISKERIKNEFHKLLEGINIQKGIYYLKKVDFFKNFFNLEIKTKVIKKI
ALLDKNKFYLKAITILTIKKPIKELKEKLTLLKFSNKEIKLILFYRGIIDNNNIFNVKKLSDIRYLLSKSTREHYKEIID
IYKALKGKNKRYLFIIKNIKRKKLLKNPLSLKDLKINGKDIQNLEQIENKNIGKILNMLLRCVIENPKLNTKNYLIKKIK
TLKVNVFHSF
>Mature_410_residues
MNLGKNNPNIIKIGKIFKKNNYEFYLVGGALRDLLLNKQPYDFDFATNATPEEIITLFPNNIKTGIKHGTIGIIFNKKIF
EITTYRIEKEYENNRAPKQVEYTKNLLKDLERRDFTINAIAMDIFNFNIIDCYNGKKDLNKKIIRCIGNPNKRLEEDALR
ILRAARFSSTLNFNIEKNTLISMKYKKENILMISKERIKNEFHKLLEGINIQKGIYYLKKVDFFKNFFNLEIKTKVIKKI
ALLDKNKFYLKAITILTIKKPIKELKEKLTLLKFSNKEIKLILFYRGIIDNNNIFNVKKLSDIRYLLSKSTREHYKEIID
IYKALKGKNKRYLFIIKNIKRKKLLKNPLSLKDLKINGKDIQNLEQIENKNIGKILNMLLRCVIENPKLNTKNYLIKKIK
TLKVNVFHSF

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=381, Percent_Identity=31.496062992126, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI87081691, Length=181, Percent_Identity=39.2265193370166, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71995920, Length=210, Percent_Identity=37.6190476190476, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI281360112, Length=211, Percent_Identity=35.5450236966825, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21357337, Length=211, Percent_Identity=35.5450236966825, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48472; Mature: 48472

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLGKNNPNIIKIGKIFKKNNYEFYLVGGALRDLLLNKQPYDFDFATNATPEEIITLFPN
CCCCCCCCCEEEEEEEEECCCCEEEEECHHHHHHHCCCCCCCCCCCCCCCHHHHEEECCC
NIKTGIKHGTIGIIFNKKIFEITTYRIEKEYENNRAPKQVEYTKNLLKDLERRDFTINAI
CHHCCCCCCEEEEEECCEEEEEEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEE
AMDIFNFNIIDCYNGKKDLNKKIIRCIGNPNKRLEEDALRILRAARFSSTLNFNIEKNTL
EEEECCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCEE
ISMKYKKENILMISKERIKNEFHKLLEGINIQKGIYYLKKVDFFKNFFNLEIKTKVIKKI
EEEEECCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALLDKNKFYLKAITILTIKKPIKELKEKLTLLKFSNKEIKLILFYRGIIDNNNIFNVKKL
HHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEEECCCCEEEEHHH
SDIRYLLSKSTREHYKEIIDIYKALKGKNKRYLFIIKNIKRKKLLKNPLSLKDLKINGKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCEEEEECCCH
IQNLEQIENKNIGKILNMLLRCVIENPKLNTKNYLIKKIKTLKVNVFHSF
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEECC
>Mature Secondary Structure
MNLGKNNPNIIKIGKIFKKNNYEFYLVGGALRDLLLNKQPYDFDFATNATPEEIITLFPN
CCCCCCCCCEEEEEEEEECCCCEEEEECHHHHHHHCCCCCCCCCCCCCCCHHHHEEECCC
NIKTGIKHGTIGIIFNKKIFEITTYRIEKEYENNRAPKQVEYTKNLLKDLERRDFTINAI
CHHCCCCCCEEEEEECCEEEEEEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEE
AMDIFNFNIIDCYNGKKDLNKKIIRCIGNPNKRLEEDALRILRAARFSSTLNFNIEKNTL
EEEECCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCEE
ISMKYKKENILMISKERIKNEFHKLLEGINIQKGIYYLKKVDFFKNFFNLEIKTKVIKKI
EEEEECCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALLDKNKFYLKAITILTIKKPIKELKEKLTLLKFSNKEIKLILFYRGIIDNNNIFNVKKL
HHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEEECCCCEEEEHHH
SDIRYLLSKSTREHYKEIIDIYKALKGKNKRYLFIIKNIKRKKLLKNPLSLKDLKINGKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCEEEEECCCH
IQNLEQIENKNIGKILNMLLRCVIENPKLNTKNYLIKKIKTLKVNVFHSF
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA