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The map label for this gene is potD [H]

Identifier: 15594984

GI number: 15594984

Start: 677892

End: 678938

Strand: Reverse

Name: potD [H]

Synonym: BB0639

Alternate gene names: 15594984

Gene position: 678938-677892 (Counterclockwise)

Preceding gene: 15594985

Following gene: 15594980

Centisome position: 74.55

GC content: 26.93

Gene sequence:

>1047_bases
ATGAAAAAAATTTTTATATTAATAGTAATTCTTACAACTTTTGCTTGCACTAACAAAGACACAATAACTTTAAACGTATT
TAATTGGGCAGAATATATTGACAAAACTTTATTAGATCAATTTGAAAAGGAAAACAATATAAAAATTAATTATGAAATCT
TTCACAATAATGAAGAAATGATGGCTAAATTTAACAACACAAAGAATTACTACGATATAATAGTCCCATCAGAATATTTA
ATCCAAGAATTAATCGATGAAGGCAAAATTGAAAAATTAGACTACTCAAAATTGCCAAATGTAACAAAAAATATTACCCA
AAATCTTACAAACTTGGAACATGATCCTGGCAATCTTTATTCAGTGCCAGCCTACTGGGGATTAATGGGCATACTTTACA
ATAAAACTAAAATAGATTTAAATGACATGCAAGGTTTTGACATATTATTTAATAAAAAATATAAAAAAGAGATTACAATG
CTAGATTCCCCTAAAGACAATATTGGGGTTGCTTTAAAAAAACTTGGATACTCAATAAATGAGCATGATACAGATAAAAT
TAAAGAAGCTGGGGAACTTTTAAAAATCCAAAATCCACTATTAATCGGATATTTTTCAGATGTGCCTGCAAAATCATTAA
TGCTAAATGGAGAAGCATCTATTCAACTCACATGGAGCGGCGAAGCACAAAGCGCTATGCTAAAAGACAAAAATTTAGAT
TTTTATGCACCTGAAAACACCAATCTATGGATAGACGCATTTGTAATTCCTATTGATGCTCCAAATAAAAACTTGGCTTA
CAAATTCATAAACTTTTTATACGAGAATGAACCATCTTATAAAAATTTCAAAGAAACTAGATATAATTCTCCAAACAAAA
ACGTAATAAAAAGAATAGAAGAAGAGGCAAAAAATAACCCCGAAATGAAATTATATTTAGAAGAAAAATTTTTACCAAAA
GATTTTTCCAAATTTGAAATTTTTAAAAAAATACCTAAAAAAATAAAAGAAGAAATCCTTAAAATATATTTAAATCTGTC
TTCTTAA

Upstream 100 bases:

>100_bases
TTTTTACAATATTGAGCCTTTTGTTTATTATTAATAAATTTATAGGAATTAAAAAATTGACAACAGATGCTGAGCTTTAA
AATGAAAAAAAGGAGTACTT

Downstream 100 bases:

>100_bases
ATATTTAAATTCAAAAAAAACTTAAAAGCTTTTAAAATTTAAAGCTTTTAAGTTTTGTGACTATTTTTAAAAAAGACTTA
AATATTAACTTAATATCAAG

Product: spermidine/putrescine ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKIFILIVILTTFACTNKDTITLNVFNWAEYIDKTLLDQFEKENNIKINYEIFHNNEEMMAKFNNTKNYYDIIVPSEYL
IQELIDEGKIEKLDYSKLPNVTKNITQNLTNLEHDPGNLYSVPAYWGLMGILYNKTKIDLNDMQGFDILFNKKYKKEITM
LDSPKDNIGVALKKLGYSINEHDTDKIKEAGELLKIQNPLLIGYFSDVPAKSLMLNGEASIQLTWSGEAQSAMLKDKNLD
FYAPENTNLWIDAFVIPIDAPNKNLAYKFINFLYENEPSYKNFKETRYNSPNKNVIKRIEEEAKNNPEMKLYLEEKFLPK
DFSKFEIFKKIPKKIKEEILKIYLNLSS

Sequences:

>Translated_348_residues
MKKIFILIVILTTFACTNKDTITLNVFNWAEYIDKTLLDQFEKENNIKINYEIFHNNEEMMAKFNNTKNYYDIIVPSEYL
IQELIDEGKIEKLDYSKLPNVTKNITQNLTNLEHDPGNLYSVPAYWGLMGILYNKTKIDLNDMQGFDILFNKKYKKEITM
LDSPKDNIGVALKKLGYSINEHDTDKIKEAGELLKIQNPLLIGYFSDVPAKSLMLNGEASIQLTWSGEAQSAMLKDKNLD
FYAPENTNLWIDAFVIPIDAPNKNLAYKFINFLYENEPSYKNFKETRYNSPNKNVIKRIEEEAKNNPEMKLYLEEKFLPK
DFSKFEIFKKIPKKIKEEILKIYLNLSS
>Mature_348_residues
MKKIFILIVILTTFACTNKDTITLNVFNWAEYIDKTLLDQFEKENNIKINYEIFHNNEEMMAKFNNTKNYYDIIVPSEYL
IQELIDEGKIEKLDYSKLPNVTKNITQNLTNLEHDPGNLYSVPAYWGLMGILYNKTKIDLNDMQGFDILFNKKYKKEITM
LDSPKDNIGVALKKLGYSINEHDTDKIKEAGELLKIQNPLLIGYFSDVPAKSLMLNGEASIQLTWSGEAQSAMLKDKNLD
FYAPENTNLWIDAFVIPIDAPNKNLAYKFINFLYENEPSYKNFKETRYNSPNKNVIKRIEEEAKNNPEMKLYLEEKFLPK
DFSKFEIFKKIPKKIKEEILKIYLNLSS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=294, Percent_Identity=34.6938775510204, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787078, Length=323, Percent_Identity=28.1733746130031, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40681; Mature: 40681

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFILIVILTTFACTNKDTITLNVFNWAEYIDKTLLDQFEKENNIKINYEIFHNNEEM
CCHHEEEHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHH
MAKFNNTKNYYDIIVPSEYLIQELIDEGKIEKLDYSKLPNVTKNITQNLTNLEHDPGNLY
HHHCCCCCCEEEEECCHHHHHHHHHCCCCEEECCHHHCCCHHHHHHHHHHCCCCCCCCEE
SVPAYWGLMGILYNKTKIDLNDMQGFDILFNKKYKKEITMLDSPKDNIGVALKKLGYSIN
ECHHHHHHHHHHHCCCEEECCCCCCCEEEECCCHHHEEEEECCCCHHHHHHHHHHCCCCC
EHDTDKIKEAGELLKIQNPLLIGYFSDVPAKSLMLNGEASIQLTWSGEAQSAMLKDKNLD
CCCHHHHHHHHHHEEECCCEEEEEECCCCHHHEEECCCCEEEEEECCCCHHHHHHCCCCC
FYAPENTNLWIDAFVIPIDAPNKNLAYKFINFLYENEPSYKNFKETRYNSPNKNVIKRIE
EECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHH
EEAKNNPEMKLYLEEKFLPKDFSKFEIFKKIPKKIKEEILKIYLNLSS
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MKKIFILIVILTTFACTNKDTITLNVFNWAEYIDKTLLDQFEKENNIKINYEIFHNNEEM
CCHHEEEHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHH
MAKFNNTKNYYDIIVPSEYLIQELIDEGKIEKLDYSKLPNVTKNITQNLTNLEHDPGNLY
HHHCCCCCCEEEEECCHHHHHHHHHCCCCEEECCHHHCCCHHHHHHHHHHCCCCCCCCEE
SVPAYWGLMGILYNKTKIDLNDMQGFDILFNKKYKKEITMLDSPKDNIGVALKKLGYSIN
ECHHHHHHHHHHHCCCEEECCCCCCCEEEECCCHHHEEEEECCCCHHHHHHHHHHCCCCC
EHDTDKIKEAGELLKIQNPLLIGYFSDVPAKSLMLNGEASIQLTWSGEAQSAMLKDKNLD
CCCHHHHHHHHHHEEECCCEEEEEECCCCHHHEEECCCCEEEEEECCCCHHHHHHCCCCC
FYAPENTNLWIDAFVIPIDAPNKNLAYKFINFLYENEPSYKNFKETRYNSPNKNVIKRIE
EECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHH
EEAKNNPEMKLYLEEKFLPKDFSKFEIFKKIPKKIKEEILKIYLNLSS
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]