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The map label for this gene is fusA1 [H]

Identifier: 15594885

GI number: 15594885

Start: 549642

End: 551723

Strand: Reverse

Name: fusA1 [H]

Synonym: BB0540

Alternate gene names: 15594885

Gene position: 551723-549642 (Counterclockwise)

Preceding gene: 15594891

Following gene: 15594884

Centisome position: 60.58

GC content: 35.25

Gene sequence:

>2082_bases
ATGGACTATAATAAATTACGAAACATAGGTATTAGCGCACACATCGACTCAGGAAAAACCACTCTTACAGAACGTATTCT
TTTTTATTGTAATAAAATTCATGCAATTCACGAAGTAAAAGGCAAAGATGGGGTTGGTGCAACAATGGACTCAATGGAAC
TTGAAAGAGAAAGAGGAATCACAATAGCATCAGCTGCAACTCACGTTGAATGGAAAGATTTTCCGATAAATATTATTGAT
ACACCCGGACACGTAGATTTTACAATTGAAGTTGAAAGATCTCTTAGAGTGCTTGACGGGGCAATATTGGTTCTTGATTC
TGTTGCAGGAGTTCAATCCCAATCAATAACTGTTGATCGACAGCTTAAAAGATATAGCGTGCCGCGCCTTGCATTTGTAA
ACAAGTGTGATAAAACCGGAGCAAATCCCTACAATGTAAAAGATCAACTAAGATCAAAACTTGACTTAAACTCCGTTTTA
ATGCAAATTCCAATTGGATTAGAAGACAAACATATTGGAGTTATAGACCTTGTATTAATGAAAGCCTACTATTTTGAAGG
AAAAGATGGAACAGAAATAATAGAAAAAGAAATACCCTCAGATCTCTTAGAAGAAGCAAAAAGCAAACGAGAAATAATGC
TTGATACTCTTGCTGACTTTAATGATGAACTTATGGAATTACACATGGAAGGAAAAGAAGTTCCTACTGAAATAATATAC
AATGCAACTAGAACAGGAACATTGGCTTTAAAATTATGCCCTGTATTTATGGGATCTGCTTATAAAAACAAAGGAGTGCA
ATTGCTCTTAGATGCTGTAACCAGATTTTTGCCATCCCCTCATGATATAAAAAACACCGCTCTTGACCTAAATAATAATG
AAAAAGAAATCGATCTTAAAATTGACAACGAGCTCCCAACTGTTGCTCTTGCATTTAAACTTGAAGACGGACAATACGGA
CAGTTGACTTATGTGAGAATCTATCAAGGAATTTTAAAAAAAGGACAAGAACTTATCAACTCAAGAACTTCTAAAAAATT
CAAAGTTGGAAGGCTTATCAGAATGCATGCCAATAATACAGAAGACATTGAATTTGGAGGAAGTGGTGACATTGTTGCTT
TATTTGGAATAGAATGTGCATCAGGAGATACGTTTTGTGATCCATCGATCAACTATTCAATGACATCAATGTTTATTCCA
GATCCAGTAATTTCTCTTTCTGTAAAACCAAAGGATAAAAAATCTGCTGATAATATGGCCAAAGCCCTTGGAAGATTTAC
AAAAGAAGATCCAACATTTAAAACTTATGTTGACATTGAATCAAATGAAACAATAATTCAAGGAATGGGAGAGCTACACT
TAGAAGTTTACATTGAAAGAATGAAAAGAGAGTTCAAGGCAGAAGTTGAAACCGGAATGCCGCAAGTAGCCTATAGAGAA
ACGATTACAAGAAAAGCTGAATTTAATTATACTCACAAAAAGCAATCTGGAGGAGCTGGTCAGTTTGGACGAGTTGCAGG
GTTTATGGAACCTCTTGACAAAGAAGGAGAAACATACGAATTTGTCAATCTAATAAAAGGAGGAGTAATCCCAACAGAAT
ATATCCCATCATGTGATAAAGGGTTCCAAAAAGCAATGGAAAGGGGAACATTAATTGGCTTTCCAATAGTTGACATAAAA
ATTACAATCAATGATGGCCAATATCACATTGTTGACTCATCTGATATTGCATTCCAATTAGCAGCAATTGGAGCTTTTAG
AGAGGCTTATGAAAAAGCAAAGCCTACAATCCTTGAGCCAATAATGAAAGTTACCCTTGAAGGACCTACTGAATTCCAAG
GCAATATGTTTGGACTTTTAAATCAAAGAAGAGGAATAATAACAGGTTCCCTAGAAGATGGAAGTTTTTCAAAAGTTGAG
GCTGAGGTGCCTTTAAGCGAAATGTTTGGATTTTCAACAGTCCTTAGATCCTCTACCCAAGGAAAAGCAGAATTCTCAAT
GGAATTCTTAAGGTATGGAAAAGTTCCAAGCACTATATTTGATGAACTTCGCAAAAAATTTAACGATCAAAACAAATCTT
AA

Upstream 100 bases:

>100_bases
AATATAAAACGATATAAGATAGATTTTTAAAAAAAAATTTTGTAAAATTACTAAGATAGTTAATCTTCTACCCACATATC
ATCAGGAGGGACAAAGTCTA

Downstream 100 bases:

>100_bases
TAAAATAATAAGGAGGCTTTATTATGATCGATTTAACACAAGAAAAACAAGAAATACTAATAAAAAACAAGTTTTTAGCC
AAAGTTTTCGGGCTTATGTC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 1 [H]

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MDYNKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELERERGITIASAATHVEWKDFPINIID
TPGHVDFTIEVERSLRVLDGAILVLDSVAGVQSQSITVDRQLKRYSVPRLAFVNKCDKTGANPYNVKDQLRSKLDLNSVL
MQIPIGLEDKHIGVIDLVLMKAYYFEGKDGTEIIEKEIPSDLLEEAKSKREIMLDTLADFNDELMELHMEGKEVPTEIIY
NATRTGTLALKLCPVFMGSAYKNKGVQLLLDAVTRFLPSPHDIKNTALDLNNNEKEIDLKIDNELPTVALAFKLEDGQYG
QLTYVRIYQGILKKGQELINSRTSKKFKVGRLIRMHANNTEDIEFGGSGDIVALFGIECASGDTFCDPSINYSMTSMFIP
DPVISLSVKPKDKKSADNMAKALGRFTKEDPTFKTYVDIESNETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
TITRKAEFNYTHKKQSGGAGQFGRVAGFMEPLDKEGETYEFVNLIKGGVIPTEYIPSCDKGFQKAMERGTLIGFPIVDIK
ITINDGQYHIVDSSDIAFQLAAIGAFREAYEKAKPTILEPIMKVTLEGPTEFQGNMFGLLNQRRGIITGSLEDGSFSKVE
AEVPLSEMFGFSTVLRSSTQGKAEFSMEFLRYGKVPSTIFDELRKKFNDQNKS

Sequences:

>Translated_693_residues
MDYNKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELERERGITIASAATHVEWKDFPINIID
TPGHVDFTIEVERSLRVLDGAILVLDSVAGVQSQSITVDRQLKRYSVPRLAFVNKCDKTGANPYNVKDQLRSKLDLNSVL
MQIPIGLEDKHIGVIDLVLMKAYYFEGKDGTEIIEKEIPSDLLEEAKSKREIMLDTLADFNDELMELHMEGKEVPTEIIY
NATRTGTLALKLCPVFMGSAYKNKGVQLLLDAVTRFLPSPHDIKNTALDLNNNEKEIDLKIDNELPTVALAFKLEDGQYG
QLTYVRIYQGILKKGQELINSRTSKKFKVGRLIRMHANNTEDIEFGGSGDIVALFGIECASGDTFCDPSINYSMTSMFIP
DPVISLSVKPKDKKSADNMAKALGRFTKEDPTFKTYVDIESNETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
TITRKAEFNYTHKKQSGGAGQFGRVAGFMEPLDKEGETYEFVNLIKGGVIPTEYIPSCDKGFQKAMERGTLIGFPIVDIK
ITINDGQYHIVDSSDIAFQLAAIGAFREAYEKAKPTILEPIMKVTLEGPTEFQGNMFGLLNQRRGIITGSLEDGSFSKVE
AEVPLSEMFGFSTVLRSSTQGKAEFSMEFLRYGKVPSTIFDELRKKFNDQNKS
>Mature_693_residues
MDYNKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELERERGITIASAATHVEWKDFPINIID
TPGHVDFTIEVERSLRVLDGAILVLDSVAGVQSQSITVDRQLKRYSVPRLAFVNKCDKTGANPYNVKDQLRSKLDLNSVL
MQIPIGLEDKHIGVIDLVLMKAYYFEGKDGTEIIEKEIPSDLLEEAKSKREIMLDTLADFNDELMELHMEGKEVPTEIIY
NATRTGTLALKLCPVFMGSAYKNKGVQLLLDAVTRFLPSPHDIKNTALDLNNNEKEIDLKIDNELPTVALAFKLEDGQYG
QLTYVRIYQGILKKGQELINSRTSKKFKVGRLIRMHANNTEDIEFGGSGDIVALFGIECASGDTFCDPSINYSMTSMFIP
DPVISLSVKPKDKKSADNMAKALGRFTKEDPTFKTYVDIESNETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
TITRKAEFNYTHKKQSGGAGQFGRVAGFMEPLDKEGETYEFVNLIKGGVIPTEYIPSCDKGFQKAMERGTLIGFPIVDIK
ITINDGQYHIVDSSDIAFQLAAIGAFREAYEKAKPTILEPIMKVTLEGPTEFQGNMFGLLNQRRGIITGSLEDGSFSKVE
AEVPLSEMFGFSTVLRSSTQGKAEFSMEFLRYGKVPSTIFDELRKKFNDQNKS

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=692, Percent_Identity=52.6011560693642, Blast_Score=713, Evalue=0.0,
Organism=Homo sapiens, GI19923640, Length=710, Percent_Identity=34.3661971830986, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI25306287, Length=693, Percent_Identity=33.7662337662338, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=40.5063291139241, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=36.3057324840764, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=40.1459854014599, Blast_Score=90, Evalue=8e-18,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=36.6666666666667, Blast_Score=85, Evalue=3e-16,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=42.4778761061947, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=42.4778761061947, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=42.4778761061947, Blast_Score=81, Evalue=4e-15,
Organism=Escherichia coli, GI1789738, Length=690, Percent_Identity=48.9855072463768, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=472, Percent_Identity=25.8474576271186, Blast_Score=159, Evalue=7e-40,
Organism=Escherichia coli, GI48994988, Length=164, Percent_Identity=40.2439024390244, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788922, Length=150, Percent_Identity=37.3333333333333, Blast_Score=104, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=682, Percent_Identity=49.4134897360704, Blast_Score=654, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17556745, Length=716, Percent_Identity=28.6312849162011, Blast_Score=258, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI17557151, Length=154, Percent_Identity=37.012987012987, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=35.8024691358025, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=35.3383458646617, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=35.3383458646617, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=689, Percent_Identity=53.9912917271408, Blast_Score=769, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322359, Length=800, Percent_Identity=30.5, Blast_Score=308, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6324707, Length=423, Percent_Identity=24.113475177305, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320593, Length=423, Percent_Identity=24.113475177305, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323320, Length=156, Percent_Identity=34.6153846153846, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=151, Percent_Identity=35.7615894039735, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582462, Length=691, Percent_Identity=51.3748191027496, Blast_Score=723, Evalue=0.0,
Organism=Drosophila melanogaster, GI221458488, Length=708, Percent_Identity=32.2033898305085, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI78706572, Length=155, Percent_Identity=35.4838709677419, Blast_Score=100, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=38.3116883116883, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=38.3116883116883, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=38.3116883116883, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=37.5, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77574; Mature: 77574

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYNKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELERERGI
CCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCE
TIASAATHVEWKDFPINIIDTPGHVDFTIEVERSLRVLDGAILVLDSVAGVQSQSITVDR
EEEEEECCCEECCCCEEEEECCCCEEEEEEECCHHHHHCCHHEEEEHHCCCCCCCEEHHH
QLKRYSVPRLAFVNKCDKTGANPYNVKDQLRSKLDLNSVLMQIPIGLEDKHIGVIDLVLM
HHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHH
KAYYFEGKDGTEIIEKEIPSDLLEEAKSKREIMLDTLADFNDELMELHMEGKEVPTEIIY
HHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHC
NATRTGTLALKLCPVFMGSAYKNKGVQLLLDAVTRFLPSPHDIKNTALDLNNNEKEIDLK
CCCCCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEE
IDNELPTVALAFKLEDGQYGQLTYVRIYQGILKKGQELINSRTSKKFKVGRLIRMHANNT
ECCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCC
EDIEFGGSGDIVALFGIECASGDTFCDPSINYSMTSMFIPDPVISLSVKPKDKKSADNMA
CCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHH
KALGRFTKEDPTFKTYVDIESNETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
HHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TITRKAEFNYTHKKQSGGAGQFGRVAGFMEPLDKEGETYEFVNLIKGGVIPTEYIPSCDK
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHCCCCHH
GFQKAMERGTLIGFPIVDIKITINDGQYHIVDSSDIAFQLAAIGAFREAYEKAKPTILEP
HHHHHHHCCCEEEEEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHH
IMKVTLEGPTEFQGNMFGLLNQRRGIITGSLEDGSFSKVEAEVPLSEMFGFSTVLRSSTQ
HHHEEECCCCCCCCCHHHHHHCCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHCCCC
GKAEFSMEFLRYGKVPSTIFDELRKKFNDQNKS
CCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDYNKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVGATMDSMELERERGI
CCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCE
TIASAATHVEWKDFPINIIDTPGHVDFTIEVERSLRVLDGAILVLDSVAGVQSQSITVDR
EEEEEECCCEECCCCEEEEECCCCEEEEEEECCHHHHHCCHHEEEEHHCCCCCCCEEHHH
QLKRYSVPRLAFVNKCDKTGANPYNVKDQLRSKLDLNSVLMQIPIGLEDKHIGVIDLVLM
HHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHH
KAYYFEGKDGTEIIEKEIPSDLLEEAKSKREIMLDTLADFNDELMELHMEGKEVPTEIIY
HHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHC
NATRTGTLALKLCPVFMGSAYKNKGVQLLLDAVTRFLPSPHDIKNTALDLNNNEKEIDLK
CCCCCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEE
IDNELPTVALAFKLEDGQYGQLTYVRIYQGILKKGQELINSRTSKKFKVGRLIRMHANNT
ECCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCC
EDIEFGGSGDIVALFGIECASGDTFCDPSINYSMTSMFIPDPVISLSVKPKDKKSADNMA
CCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHH
KALGRFTKEDPTFKTYVDIESNETIIQGMGELHLEVYIERMKREFKAEVETGMPQVAYRE
HHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TITRKAEFNYTHKKQSGGAGQFGRVAGFMEPLDKEGETYEFVNLIKGGVIPTEYIPSCDK
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHCCCCHH
GFQKAMERGTLIGFPIVDIKITINDGQYHIVDSSDIAFQLAAIGAFREAYEKAKPTILEP
HHHHHHHCCCEEEEEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHH
IMKVTLEGPTEFQGNMFGLLNQRRGIITGSLEDGSFSKVEAEVPLSEMFGFSTVLRSSTQ
HHHEEECCCCCCCCCHHHHHHCCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHCCCC
GKAEFSMEFLRYGKVPSTIFDELRKKFNDQNKS
CCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA