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The map label for this gene is grpE [H]

Identifier: 15594864

GI number: 15594864

Start: 531129

End: 531692

Strand: Reverse

Name: grpE [H]

Synonym: BB0519

Alternate gene names: 15594864

Gene position: 531692-531129 (Counterclockwise)

Preceding gene: 15594865

Following gene: 15594863

Centisome position: 58.38

GC content: 25.53

Gene sequence:

>564_bases
ATGGAAAAAAAAGAAACTAAAAGCGAATCTGAAAAAACCAACAAACAAGATAATAAAAATACAAAATCTCAAAAAAAAGA
AAACTTAAATTTAGTAAATTCTGATAAAAAAATTGCTGAACTTGAAAATGAAATCTCCAATCTTAAAGATTTGTATTTAA
GAAAACAAGCAGAATTTGAAAACTTCAGAAAAAGATTAGAAAAAGAAAAAGACAATTTTGTTAAATTTGCAAATGAAACC
ATAATGAAAGATGTCGTTAATTTTCTTGATAACTTAGAACGAGCTATTAACTCTTCAAAAAAATCTAAAGATTTTGATAA
CTTATTAACAGGAATCAGTATGATTGAAAATGAAATACTATCAATCTTTGATAAAAAATATAATTTAAAAAAATTTGGAG
AAAATGGCGAGAACTTTGATCCAAGTCGTCATGAAGCAATAAGCATTGAAGAGAAAGAGGGTCTTAAAAATCCAGAAATA
GTAGAAGTATACCAAAAAGGATATTGCTACAACGACAGAATATTAAGAACAGCGAAAGTTAAAGTTGCTCAAAGCAAAAA
TTAA

Upstream 100 bases:

>100_bases
ACAAATGCTCAAAATGAATCAATTTTATTAAATTTCAAGAAAGAAAAAAATTTGTAAAATATTTTTTTTAAAGATAAAAT
GCTTTATCAAAGGAGGAAAA

Downstream 100 bases:

>100_bases
TTAGAAAAAAGGAGGTTTATAATATGGGCAAAATAATAGGTATTGACCTAGGAACAACAAACTCATGCGTAGCTATAATG
GAGCATGGAAAACCAGTTGT

Product: grpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MEKKETKSESEKTNKQDNKNTKSQKKENLNLVNSDKKIAELENEISNLKDLYLRKQAEFENFRKRLEKEKDNFVKFANET
IMKDVVNFLDNLERAINSSKKSKDFDNLLTGISMIENEILSIFDKKYNLKKFGENGENFDPSRHEAISIEEKEGLKNPEI
VEVYQKGYCYNDRILRTAKVKVAQSKN

Sequences:

>Translated_187_residues
MEKKETKSESEKTNKQDNKNTKSQKKENLNLVNSDKKIAELENEISNLKDLYLRKQAEFENFRKRLEKEKDNFVKFANET
IMKDVVNFLDNLERAINSSKKSKDFDNLLTGISMIENEILSIFDKKYNLKKFGENGENFDPSRHEAISIEEKEGLKNPEI
VEVYQKGYCYNDRILRTAKVKVAQSKN
>Mature_187_residues
MEKKETKSESEKTNKQDNKNTKSQKKENLNLVNSDKKIAELENEISNLKDLYLRKQAEFENFRKRLEKEKDNFVKFANET
IMKDVVNFLDNLERAINSSKKSKDFDNLLTGISMIENEILSIFDKKYNLKKFGENGENFDPSRHEAISIEEKEGLKNPEI
VEVYQKGYCYNDRILRTAKVKVAQSKN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI40255109, Length=155, Percent_Identity=30.9677419354839, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1788967, Length=157, Percent_Identity=30.5732484076433, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552458, Length=152, Percent_Identity=32.8947368421053, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324806, Length=190, Percent_Identity=32.6315789473684, Blast_Score=78, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24653432, Length=155, Percent_Identity=30.3225806451613, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21939; Mature: 21939

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKETKSESEKTNKQDNKNTKSQKKENLNLVNSDKKIAELENEISNLKDLYLRKQAEFE
CCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFRKRLEKEKDNFVKFANETIMKDVVNFLDNLERAINSSKKSKDFDNLLTGISMIENEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SIFDKKYNLKKFGENGENFDPSRHEAISIEEKEGLKNPEIVEVYQKGYCYNDRILRTAKV
HHHHHHCCHHHHCCCCCCCCCCCHHEECCHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHH
KVAQSKN
HHHCCCC
>Mature Secondary Structure
MEKKETKSESEKTNKQDNKNTKSQKKENLNLVNSDKKIAELENEISNLKDLYLRKQAEFE
CCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFRKRLEKEKDNFVKFANETIMKDVVNFLDNLERAINSSKKSKDFDNLLTGISMIENEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SIFDKKYNLKKFGENGENFDPSRHEAISIEEKEGLKNPEIVEVYQKGYCYNDRILRTAKV
HHHHHHCCHHHHCCCCCCCCCCCHHEECCHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHH
KVAQSKN
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA