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The map label for this gene is rpoN [H]

Identifier: 15594795

GI number: 15594795

Start: 469961

End: 471220

Strand: Reverse

Name: rpoN [H]

Synonym: BB0450

Alternate gene names: 15594795

Gene position: 471220-469961 (Counterclockwise)

Preceding gene: 15594796

Following gene: 15594794

Centisome position: 51.74

GC content: 25.4

Gene sequence:

>1260_bases
ATGATTAAACAAAAATTAAAAATATCTCAAAATTTAAACTCAATTCAAATACAAACAATAAAAATATTAAGCCTTAACCA
AAAAGAATTAACAAAGCTTATACTAGAAGAAAGCGAAAATAATGAATGTCTAGAAATAAACTCAAATAAAATATTTTTTG
AAACATTGAAAACATATAGGTTTAAAAAAGTTTTTTATAAAGAAGATGATATGATAAAAAATCAACACGACATAGCTCTT
GAAAAAACACAAACAAATACTTCTTTAAAAGAACACCTTTTACTGCAATTAAGAATTCAAAGAATAAATGAAGATGAAAT
TAAAATAGGCGAAATACTCATAAACAATCTAAACAGCAAAGGTTTTCATATAATAAACCCTTACGATCTTTTTAAAAAGG
AAGAAAAAGAAAAAGTAAAAAAAATAATTGAACTTATTCAAAAATTTGATCCAATTGGAATTTGTGTCCCCAACATAATA
GAATCGTTAATTTTGCAAGCAAAGCATCATAAATTAGAAACTAATATTATTAAAATTCTTGAAAAAGCAGAGCTTCTTGA
AAAAACTCAAAAAAAGTTAAAAGAGGAACTTAAAATAAGAAGCAAAGAATTTAACACGGCTTTAGAAATTATCAGACAAA
AACTTAACCCCAACCCAACGCTCGAATTTAAAGACCCAAACGACACTAATTTTTATGTTGATCCAGATATATTAATAATA
AATCACAATAATAAATTTAAAATTAAAATCAAAGAAGTTAATATCTTTAAAAAAGAACTTAAAAGGACAAGTGAAAACCC
CCAAAAACAAAAAAAAGCAAAGTGGTTAATCGAATCCCTACGATATAGAGACGAAATACTTGCAAAAATAGGAATAGCTA
TATATACATTGCAAAAAGAATTTCTAAGAAGAGGATTTAAAAGCTTAAGGCCAATGAACTTGAGCATTTTATCAGAAAAA
ATTAGTGTATCAAAATCAACAATATCAAGAGCAATAAAAAATAAATACTTAAAATGCGAATGGGGTACAATATTAATCAA
AGAGCTTTTTAGCTCTGTTGGTGGAGCAAAAACAAATGAATTTTCAAAATTAAGCATCAAAATAACAGTAAAAAAGTTAT
TAGAAGCAAATAAAAAGATGTCAGACAAAGAGATTTCTGTTATACTAAAGTCCAAAGGAATCTCTATTTCTAGAAGAACA
GTAAATAAATACAGAAATGAATTAAAATCTGAGAAAGGGAGAACATATTATGGAACCTAA

Upstream 100 bases:

>100_bases
CTATTGTAGGAATTAATTTTTTTATTTTATTTTTTTATAATAAAATAAGTCCAGCATTAGTAATTATACTTAGTGGATTT
TTTTATACATTAATATAAAT

Downstream 100 bases:

>100_bases
AATTCAAACGGTTAATTACAGCTTGAATGAGAATGAAAAAAATTTTATTCTCAAAAAGCTAGAAAAATTTGATACTCATA
TCAAAAAACATATTGATAAT

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MIKQKLKISQNLNSIQIQTIKILSLNQKELTKLILEESENNECLEINSNKIFFETLKTYRFKKVFYKEDDMIKNQHDIAL
EKTQTNTSLKEHLLLQLRIQRINEDEIKIGEILINNLNSKGFHIINPYDLFKKEEKEKVKKIIELIQKFDPIGICVPNII
ESLILQAKHHKLETNIIKILEKAELLEKTQKKLKEELKIRSKEFNTALEIIRQKLNPNPTLEFKDPNDTNFYVDPDILII
NHNNKFKIKIKEVNIFKKELKRTSENPQKQKKAKWLIESLRYRDEILAKIGIAIYTLQKEFLRRGFKSLRPMNLSILSEK
ISVSKSTISRAIKNKYLKCEWGTILIKELFSSVGGAKTNEFSKLSIKITVKKLLEANKKMSDKEISVILKSKGISISRRT
VNKYRNELKSEKGRTYYGT

Sequences:

>Translated_419_residues
MIKQKLKISQNLNSIQIQTIKILSLNQKELTKLILEESENNECLEINSNKIFFETLKTYRFKKVFYKEDDMIKNQHDIAL
EKTQTNTSLKEHLLLQLRIQRINEDEIKIGEILINNLNSKGFHIINPYDLFKKEEKEKVKKIIELIQKFDPIGICVPNII
ESLILQAKHHKLETNIIKILEKAELLEKTQKKLKEELKIRSKEFNTALEIIRQKLNPNPTLEFKDPNDTNFYVDPDILII
NHNNKFKIKIKEVNIFKKELKRTSENPQKQKKAKWLIESLRYRDEILAKIGIAIYTLQKEFLRRGFKSLRPMNLSILSEK
ISVSKSTISRAIKNKYLKCEWGTILIKELFSSVGGAKTNEFSKLSIKITVKKLLEANKKMSDKEISVILKSKGISISRRT
VNKYRNELKSEKGRTYYGT
>Mature_419_residues
MIKQKLKISQNLNSIQIQTIKILSLNQKELTKLILEESENNECLEINSNKIFFETLKTYRFKKVFYKEDDMIKNQHDIAL
EKTQTNTSLKEHLLLQLRIQRINEDEIKIGEILINNLNSKGFHIINPYDLFKKEEKEKVKKIIELIQKFDPIGICVPNII
ESLILQAKHHKLETNIIKILEKAELLEKTQKKLKEELKIRSKEFNTALEIIRQKLNPNPTLEFKDPNDTNFYVDPDILII
NHNNKFKIKIKEVNIFKKELKRTSENPQKQKKAKWLIESLRYRDEILAKIGIAIYTLQKEFLRRGFKSLRPMNLSILSEK
ISVSKSTISRAIKNKYLKCEWGTILIKELFSSVGGAKTNEFSKLSIKITVKKLLEANKKMSDKEISVILKSKGISISRRT
VNKYRNELKSEKGRTYYGT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=416, Percent_Identity=25.7211538461538, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 49160; Mature: 49160

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQKLKISQNLNSIQIQTIKILSLNQKELTKLILEESENNECLEINSNKIFFETLKTYR
CCCCHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCEEEECCCEEEHHHHHHHH
FKKVFYKEDDMIKNQHDIALEKTQTNTSLKEHLLLQLRIQRINEDEIKIGEILINNLNSK
HHHHHCCCCHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
GFHIINPYDLFKKEEKEKVKKIIELIQKFDPIGICVPNIIESLILQAKHHKLETNIIKIL
CEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
EKAELLEKTQKKLKEELKIRSKEFNTALEIIRQKLNPNPTLEFKDPNDTNFYVDPDILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEECCEEEEE
NHNNKFKIKIKEVNIFKKELKRTSENPQKQKKAKWLIESLRYRDEILAKIGIAIYTLQKE
ECCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRRGFKSLRPMNLSILSEKISVSKSTISRAIKNKYLKCEWGTILIKELFSSVGGAKTNE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCC
FSKLSIKITVKKLLEANKKMSDKEISVILKSKGISISRRTVNKYRNELKSEKGRTYYGT
CEEEEEEEEHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIKQKLKISQNLNSIQIQTIKILSLNQKELTKLILEESENNECLEINSNKIFFETLKTYR
CCCCHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCEEEECCCEEEHHHHHHHH
FKKVFYKEDDMIKNQHDIALEKTQTNTSLKEHLLLQLRIQRINEDEIKIGEILINNLNSK
HHHHHCCCCHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
GFHIINPYDLFKKEEKEKVKKIIELIQKFDPIGICVPNIIESLILQAKHHKLETNIIKIL
CEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
EKAELLEKTQKKLKEELKIRSKEFNTALEIIRQKLNPNPTLEFKDPNDTNFYVDPDILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEECCEEEEE
NHNNKFKIKIKEVNIFKKELKRTSENPQKQKKAKWLIESLRYRDEILAKIGIAIYTLQKE
ECCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRRGFKSLRPMNLSILSEKISVSKSTISRAIKNKYLKCEWGTILIKELFSSVGGAKTNE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCC
FSKLSIKITVKKLLEANKKMSDKEISVILKSKGISISRRTVNKYRNELKSEKGRTYYGT
CEEEEEEEEHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3481423 [H]