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The map label for this gene is proS [H]

Identifier: 15594747

GI number: 15594747

Start: 414327

End: 415793

Strand: Reverse

Name: proS [H]

Synonym: BB0402

Alternate gene names: 15594747

Gene position: 415793-414327 (Counterclockwise)

Preceding gene: 15594748

Following gene: 15594745

Centisome position: 45.66

GC content: 33.27

Gene sequence:

>1467_bases
ATGAGTGATTTTATAGCATCAAAAGAAGATGATTATTCTAAATGGTATTTAGATATAGTCCAGAAAGCAAAACTTGCTGA
TTACAGTCCTGTAAAAGGATGTATGGTAATTATGCCTTATGGGTATTCTATTTGGAGTAAAATTCAGAGTATACTTGATA
AAAAATTTAAAGAGACGGGACACGAGAATGCATATTTCCCTATGCTTATTCCTTATAGTTTTTTAGAAAGAGAAAAGGAT
CATATTGATGGATTTTCACCCGAGTTTGCTATTATTAAGGATGCTGGTGGAGAGAGTTTGGCAGAGCCTTTGGTTTTAAG
GCCTACCTCTGAGACAATTATTTGGAATATGTATAGTAAGTGGATTAAGTCTTACAGAGATCTTCCTCTTAAAATTAATC
AATGGGCAAATGTTGTTCGTTGGGAAAAGAGAACAAGGCCTTTTTTGCGCACTACCGAATTTCTATGGCAAGAAGGACAT
ACTGCTCATGCTACCGAAGAGGAGGCATTAGAAGAAACTTTACTTATTTTAGATGTATATAAAAGATTCATAGAAGACTA
TTTGGCCATTCCGGTGTTTTGTGGTAAAAAATCTGAAAAGGAAAAATTTGCGGGGGCTGTTTCTACTTATTCAATTGAGG
CATTAATGCAAGATAAAAAAGCGCTTCAAGCCGCTACATCCCATTATTTAGGTTTAAATTTTGCAAAGGCATTTGATGTA
AAATTTCAAGACAAAGATGGCAAGATGCGGCATGTATTTGCTAGTAGCTGGGGTGTTTCTACCAGATTGATTGGTGCTTT
GATTATGGTTCATTCTGATGAGAAAGGTTTAGTTTTGCCGCCTCGCATTGCTCCAATAGAAATTATTGTTATTCCTATTT
TTAAAAAAGAAGATGAGATTAATAAAAAAATTTTAGATTATTCTGATTGTGTTGTGGATGCTTTAAAAAAAGCAGAATTT
AGGGTTGAAATTGATAAGGACGTTAGAAGTTCTCCGGGATTTAGATTTTCATCTGCCGAGTTTAAAGGAATTCCAATACG
CCTTGAAGTGGGGATAAATGATGTCCTTTTAAATTCCGTTACTATTTCAAGAAGAGATAAAGACAGAAAATTTAAGTATC
AAATATCACTTGATTCTCTTATAAGCAAGGTTAAGGTAGAGCTTGATTTGATGCAAAAAGATTTATTTCAAAGAGCATTA
AATTTTAGGATCTTGAATACTAAGGAGATTTTTAGAAGCAGTAAGGATAGTTATGAGACATTCAAAGCCTATGTGAATGA
TTATTCTGGATTTGTGCTTTCTTGTTGGTGTGGCAGTTTGAATTGTGAAAATATTATTAAAAATGAAACTAAAGCCACAA
TAAGATGTATCCCCGATGATTTTAAGGCCAGAGATTTAACAGGCATGACTTGTATTTATTGTTCATCTAAAGCTAAATAT
TTTGTTTTATTTGCCAAATCCTATTAA

Upstream 100 bases:

>100_bases
TATGGAAATAGGTATAAACGGTTTATGGTTGAAGTTAAAAAATATTGAATATAATTATTTAGTTTTTTAAAGAAGTGAAA
TGCTTTATTGAGGATAATTT

Downstream 100 bases:

>100_bases
TTTGTTTAGCTTTAATTAATTTTTTCTTGATCTTTTAATTCTTTGAAGTTTATTATATGAATGATTGCAATGTTAAAAGC
AAAGTCGATTAGTAAAGCCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSDFIASKEDDYSKWYLDIVQKAKLADYSPVKGCMVIMPYGYSIWSKIQSILDKKFKETGHENAYFPMLIPYSFLEREKD
HIDGFSPEFAIIKDAGGESLAEPLVLRPTSETIIWNMYSKWIKSYRDLPLKINQWANVVRWEKRTRPFLRTTEFLWQEGH
TAHATEEEALEETLLILDVYKRFIEDYLAIPVFCGKKSEKEKFAGAVSTYSIEALMQDKKALQAATSHYLGLNFAKAFDV
KFQDKDGKMRHVFASSWGVSTRLIGALIMVHSDEKGLVLPPRIAPIEIIVIPIFKKEDEINKKILDYSDCVVDALKKAEF
RVEIDKDVRSSPGFRFSSAEFKGIPIRLEVGINDVLLNSVTISRRDKDRKFKYQISLDSLISKVKVELDLMQKDLFQRAL
NFRILNTKEIFRSSKDSYETFKAYVNDYSGFVLSCWCGSLNCENIIKNETKATIRCIPDDFKARDLTGMTCIYCSSKAKY
FVLFAKSY

Sequences:

>Translated_488_residues
MSDFIASKEDDYSKWYLDIVQKAKLADYSPVKGCMVIMPYGYSIWSKIQSILDKKFKETGHENAYFPMLIPYSFLEREKD
HIDGFSPEFAIIKDAGGESLAEPLVLRPTSETIIWNMYSKWIKSYRDLPLKINQWANVVRWEKRTRPFLRTTEFLWQEGH
TAHATEEEALEETLLILDVYKRFIEDYLAIPVFCGKKSEKEKFAGAVSTYSIEALMQDKKALQAATSHYLGLNFAKAFDV
KFQDKDGKMRHVFASSWGVSTRLIGALIMVHSDEKGLVLPPRIAPIEIIVIPIFKKEDEINKKILDYSDCVVDALKKAEF
RVEIDKDVRSSPGFRFSSAEFKGIPIRLEVGINDVLLNSVTISRRDKDRKFKYQISLDSLISKVKVELDLMQKDLFQRAL
NFRILNTKEIFRSSKDSYETFKAYVNDYSGFVLSCWCGSLNCENIIKNETKATIRCIPDDFKARDLTGMTCIYCSSKAKY
FVLFAKSY
>Mature_487_residues
SDFIASKEDDYSKWYLDIVQKAKLADYSPVKGCMVIMPYGYSIWSKIQSILDKKFKETGHENAYFPMLIPYSFLEREKDH
IDGFSPEFAIIKDAGGESLAEPLVLRPTSETIIWNMYSKWIKSYRDLPLKINQWANVVRWEKRTRPFLRTTEFLWQEGHT
AHATEEEALEETLLILDVYKRFIEDYLAIPVFCGKKSEKEKFAGAVSTYSIEALMQDKKALQAATSHYLGLNFAKAFDVK
FQDKDGKMRHVFASSWGVSTRLIGALIMVHSDEKGLVLPPRIAPIEIIVIPIFKKEDEINKKILDYSDCVVDALKKAEFR
VEIDKDVRSSPGFRFSSAEFKGIPIRLEVGINDVLLNSVTISRRDKDRKFKYQISLDSLISKVKVELDLMQKDLFQRALN
FRILNTKEIFRSSKDSYETFKAYVNDYSGFVLSCWCGSLNCENIIKNETKATIRCIPDDFKARDLTGMTCIYCSSKAKYF
VLFAKSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=512, Percent_Identity=40.0390625, Blast_Score=374, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71991342, Length=499, Percent_Identity=42.4849699398798, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71991346, Length=361, Percent_Identity=47.3684210526316, Blast_Score=354, Evalue=7e-98,
Organism=Saccharomyces cerevisiae, GI6321808, Length=513, Percent_Identity=42.3001949317739, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24649468, Length=505, Percent_Identity=38.6138613861386, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24649466, Length=505, Percent_Identity=38.6138613861386, Blast_Score=365, Evalue=1e-101,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 56312; Mature: 56180

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFIASKEDDYSKWYLDIVQKAKLADYSPVKGCMVIMPYGYSIWSKIQSILDKKFKETG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCC
HENAYFPMLIPYSFLEREKDHIDGFSPEFAIIKDAGGESLAEPLVLRPTSETIIWNMYSK
CCCCCCEEECCHHHHHCCHHHCCCCCCCEEEEECCCCCHHCCCEEECCCCCCEEHHHHHH
WIKSYRDLPLKINQWANVVRWEKRTRPFLRTTEFLWQEGHTAHATEEEALEETLLILDVY
HHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KRFIEDYLAIPVFCGKKSEKEKFAGAVSTYSIEALMQDKKALQAATSHYLGLNFAKAFDV
HHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
KFQDKDGKMRHVFASSWGVSTRLIGALIMVHSDEKGLVLPPRIAPIEIIVIPIFKKEDEI
EEECCCCCEEEEEECCCCHHHHHHHEEEEEECCCCCEEECCCCCCEEEEEEEEECCCCHH
NKKILDYSDCVVDALKKAEFRVEIDKDVRSSPGFRFSSAEFKGIPIRLEVGINDVLLNSV
HHHHHCHHHHHHHHHHHCCEEEEECCCCCCCCCCEECCCCCCCCCEEEEECCCHHHEEEE
TISRRDKDRKFKYQISLDSLISKVKVELDLMQKDLFQRALNFRILNTKEIFRSSKDSYET
EEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHCCCCHHHH
FKAYVNDYSGFVLSCWCGSLNCENIIKNETKATIRCIPDDFKARDLTGMTCIYCSSKAKY
HHHHHHCCCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCEE
FVLFAKSY
EEEEEECC
>Mature Secondary Structure 
SDFIASKEDDYSKWYLDIVQKAKLADYSPVKGCMVIMPYGYSIWSKIQSILDKKFKETG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCC
HENAYFPMLIPYSFLEREKDHIDGFSPEFAIIKDAGGESLAEPLVLRPTSETIIWNMYSK
CCCCCCEEECCHHHHHCCHHHCCCCCCCEEEEECCCCCHHCCCEEECCCCCCEEHHHHHH
WIKSYRDLPLKINQWANVVRWEKRTRPFLRTTEFLWQEGHTAHATEEEALEETLLILDVY
HHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KRFIEDYLAIPVFCGKKSEKEKFAGAVSTYSIEALMQDKKALQAATSHYLGLNFAKAFDV
HHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
KFQDKDGKMRHVFASSWGVSTRLIGALIMVHSDEKGLVLPPRIAPIEIIVIPIFKKEDEI
EEECCCCCEEEEEECCCCHHHHHHHEEEEEECCCCCEEECCCCCCEEEEEEEEECCCCHH
NKKILDYSDCVVDALKKAEFRVEIDKDVRSSPGFRFSSAEFKGIPIRLEVGINDVLLNSV
HHHHHCHHHHHHHHHHHCCEEEEECCCCCCCCCCEECCCCCCCCCEEEEECCCHHHEEEE
TISRRDKDRKFKYQISLDSLISKVKVELDLMQKDLFQRALNFRILNTKEIFRSSKDSYET
EEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHCCCCHHHH
FKAYVNDYSGFVLSCWCGSLNCENIIKNETKATIRCIPDDFKARDLTGMTCIYCSSKAKY
HHHHHHCCCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCEE
FVLFAKSY
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA