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The map label for this gene is treA [C]

Identifier: 15594726

GI number: 15594726

Start: 390392

End: 391819

Strand: Reverse

Name: treA [C]

Synonym: BB0381

Alternate gene names: 15594726

Gene position: 391819-390392 (Counterclockwise)

Preceding gene: 15594727

Following gene: 15594725

Centisome position: 43.02

GC content: 28.52

Gene sequence:

>1428_bases
TTGAATAAAAAAATGTTTCCCAAAATTTACTATTATGATCAAGACTTTATTGATATTTATAATAAAAGTTTATCTTGGAT
TCAAGATAAGGTGATTTTGCAAAAAGTTGCTGATAGGGGCAAAAAAGATAAAAATTATTATTCGGAAAATTGTGATTATA
TAGATCAGATGCAAGCTTGTATGTCAAGCTTTTTTCTTGTCTATAGTAATGGGGAATATTCATCTACATCTGCAATTGAT
AAATTTTATCAATTGCAGGAAGAATCTGGTGCAATCAGGGCTAGATATGACAATAACAATGCTATTATTGATCTTGATGA
GAATGAAGAGAATATTGGATTTCCTATTTTTGCATGGGCTGAATACAACTTATATCATAAGACAGGAAATAAAAAGAGAA
TTTCTGAAGTTTTACCAATTCTTGATAAGTATTATAAATGGATAGAGAGCAAATTTTTAAAGGAAAATGGTCTTTATTCA
ATTGATGTAAATAAAATTTTTTATAAGAACTCCCCAAGAGTAGATGCGTACTATCCTATAGATTTTAATTCATTGCAAGT
TCATAATGCATATTGTATTTCTAAATTGGCAGACATTTTAAATGATAAAAATTTATCGCTTGAATACAAAAAACGATTTT
TTTCCCTTAAGGTCAAAATTAATTCTTTAATGTGGAGCGAAAAAGATGGATTTTATTATGATCTTGATGTTAATGAAAAT
ATTCTTGAAATCAAGACAATAGTAGGTTTTTTCCCAATGCTTTCTGAGATTCCCAGTGAGGACAGAATAGAAAGAATGAT
TTTTTATTTAAAAAGTACTAATCATTTTGGGACTCCAAATCCTTTTCCAACACTTTCTGTTAGTGAGCCAGGTTTTAGTG
AGGATGGCAATGGATATTATGGTTCAGTTTATACTTATATGAATTTTTTTGTAATCAAAGGGCTTGAATATTGTGGTCGT
GCAAATATTGCAAGAGAATTTACTATAAGACATTTGTATTATATATTAGACACTTTAATGCCTTTTAATAAAATTAAGGG
GCACATTTGGGAAGCTTATAGACCTATGCAAGAAGGACCTGCATATTTTGATTCTAATAAAAAAACTTATACCGAGAAAG
GTCTTATTTGTTATCTTGCACTTTTTAGTATTAGCTTAATGATAGAAAATATTATTGGGCTTACAATTAGTTTGCCTGAT
AAAACTGTATATTGGAATATACCCACTCTTGAGATTATGGGGATAGAAAGCTTATCTCTTAAAAAGAATCAAACTACAAT
AATTTGCAATAAGGGGAAAAGAGGTTGGGAAATAAAAATGGAATCTGAAAAACTTTATTATTTTACAATAAATATATTAA
ATAAAAAAGAAAAAACCCTTCCTATTCCCTCAGGAAGATGTTCTATGTTATTGGATAAGCTTKGATGA

Upstream 100 bases:

>100_bases
GAAAGATTTTAATTTTCCAGTTTTTAATTTTTTAATTATGTTATATTTATTGTGTTATAATAAATAGAAGTACATTTCTG
TTGTTTTAGAGGATTTAGCA

Downstream 100 bases:

>100_bases
ATTAGATTCATTAAATGTCTTGTAAAATTTAAATTTTTGGAGGCCTTTTAATGATAGATATTGATGAGTTGAGAATTTTT
CTTAAAGAGAAGAGCTATTC

Product: hypothetical protein

Products: D-Glucose [C]

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNKKMFPKIYYYDQDFIDIYNKSLSWIQDKVILQKVADRGKKDKNYYSENCDYIDQMQACMSSFFLVYSNGEYSSTSAID
KFYQLQEESGAIRARYDNNNAIIDLDENEENIGFPIFAWAEYNLYHKTGNKKRISEVLPILDKYYKWIESKFLKENGLYS
IDVNKIFYKNSPRVDAYYPIDFNSLQVHNAYCISKLADILNDKNLSLEYKKRFFSLKVKINSLMWSEKDGFYYDLDVNEN
ILEIKTIVGFFPMLSEIPSEDRIERMIFYLKSTNHFGTPNPFPTLSVSEPGFSEDGNGYYGSVYTYMNFFVIKGLEYCGR
ANIAREFTIRHLYYILDTLMPFNKIKGHIWEAYRPMQEGPAYFDSNKKTYTEKGLICYLALFSISLMIENIIGLTISLPD
KTVYWNIPTLEIMGIESLSLKKNQTTIICNKGKRGWEIKMESEKLYYFTINILNKKEKTLPIPSGRCSMLLDKLX

Sequences:

>Translated_475_residues
MNKKMFPKIYYYDQDFIDIYNKSLSWIQDKVILQKVADRGKKDKNYYSENCDYIDQMQACMSSFFLVYSNGEYSSTSAID
KFYQLQEESGAIRARYDNNNAIIDLDENEENIGFPIFAWAEYNLYHKTGNKKRISEVLPILDKYYKWIESKFLKENGLYS
IDVNKIFYKNSPRVDAYYPIDFNSLQVHNAYCISKLADILNDKNLSLEYKKRFFSLKVKINSLMWSEKDGFYYDLDVNEN
ILEIKTIVGFFPMLSEIPSEDRIERMIFYLKSTNHFGTPNPFPTLSVSEPGFSEDGNGYYGSVYTYMNFFVIKGLEYCGR
ANIAREFTIRHLYYILDTLMPFNKIKGHIWEAYRPMQEGPAYFDSNKKTYTEKGLICYLALFSISLMIENIIGLTISLPD
KTVYWNIPTLEIMGIESLSLKKNQTTIICNKGKRGWEIKMESEKLYYFTINILNKKEKTLPIPSGRCSMLLDKLX
>Mature_475_residues
MNKKMFPKIYYYDQDFIDIYNKSLSWIQDKVILQKVADRGKKDKNYYSENCDYIDQMQACMSSFFLVYSNGEYSSTSAID
KFYQLQEESGAIRARYDNNNAIIDLDENEENIGFPIFAWAEYNLYHKTGNKKRISEVLPILDKYYKWIESKFLKENGLYS
IDVNKIFYKNSPRVDAYYPIDFNSLQVHNAYCISKLADILNDKNLSLEYKKRFFSLKVKINSLMWSEKDGFYYDLDVNEN
ILEIKTIVGFFPMLSEIPSEDRIERMIFYLKSTNHFGTPNPFPTLSVSEPGFSEDGNGYYGSVYTYMNFFVIKGLEYCGR
ANIAREFTIRHLYYILDTLMPFNKIKGHIWEAYRPMQEGPAYFDSNKKTYTEKGLICYLALFSISLMIENIIGLTISLPD
KTVYWNIPTLEIMGIESLSLKKNQTTIICNKGKRGWEIKMESEKLYYFTINILNKKEKTLPIPSGRCSMLLDKLX

Specific function: Provides The Cells With The Ability To Utilize Trehalose At High Osmolarity By Splitting It Into Glucose Molecules That Can Subsequently Be Taken Up By The Phosphotransferase-Mediated Uptake System. [C]

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR001661 [H]

Pfam domain/function: PF01204 Trehalase [H]

EC number: 3.2.1.28 [C]

Molecular weight: Translated: 55731; Mature: 55731

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKMFPKIYYYDQDFIDIYNKSLSWIQDKVILQKVADRGKKDKNYYSENCDYIDQMQAC
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHH
MSSFFLVYSNGEYSSTSAIDKFYQLQEESGAIRARYDNNNAIIDLDENEENIGFPIFAWA
HHCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEE
EYNLYHKTGNKKRISEVLPILDKYYKWIESKFLKENGLYSIDVNKIFYKNSPRVDAYYPI
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEEECCCCCCEEEECC
DFNSLQVHNAYCISKLADILNDKNLSLEYKKRFFSLKVKINSLMWSEKDGFYYDLDVNEN
CCCCEEECHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEEEECCCCEEEEECCCCC
ILEIKTIVGFFPMLSEIPSEDRIERMIFYLKSTNHFGTPNPFPTLSVSEPGFSEDGNGYY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCCCEEEECCCCCCCCCCCEE
GSVYTYMNFFVIKGLEYCGRANIAREFTIRHLYYILDTLMPFNKIKGHIWEAYRPMQEGP
EHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCC
AYFDSNKKTYTEKGLICYLALFSISLMIENIIGLTISLPDKTVYWNIPTLEIMGIESLSL
CEECCCCCEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCEEEEECCCCEEE
KKNQTTIICNKGKRGWEIKMESEKLYYFTINILNKKEKTLPIPSGRCSMLLDKLX
CCCCEEEEECCCCCCEEEEEECCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNKKMFPKIYYYDQDFIDIYNKSLSWIQDKVILQKVADRGKKDKNYYSENCDYIDQMQAC
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHH
MSSFFLVYSNGEYSSTSAIDKFYQLQEESGAIRARYDNNNAIIDLDENEENIGFPIFAWA
HHCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEE
EYNLYHKTGNKKRISEVLPILDKYYKWIESKFLKENGLYSIDVNKIFYKNSPRVDAYYPI
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEEECCCCCCEEEECC
DFNSLQVHNAYCISKLADILNDKNLSLEYKKRFFSLKVKINSLMWSEKDGFYYDLDVNEN
CCCCEEECHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEEEECCCCEEEEECCCCC
ILEIKTIVGFFPMLSEIPSEDRIERMIFYLKSTNHFGTPNPFPTLSVSEPGFSEDGNGYY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCCCEEEECCCCCCCCCCCEE
GSVYTYMNFFVIKGLEYCGRANIAREFTIRHLYYILDTLMPFNKIKGHIWEAYRPMQEGP
EHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCC
AYFDSNKKTYTEKGLICYLALFSISLMIENIIGLTISLPDKTVYWNIPTLEIMGIESLSL
CEECCCCCEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCEEEEECCCCEEE
KKNQTTIICNKGKRGWEIKMESEKLYYFTINILNKKEKTLPIPSGRCSMLLDKLX
CCCCEEEEECCCCCCEEEEEECCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {trehalose}} [C]

Substrates: H2O; Trehalose [C]

Specific reaction: H2O + Trehalose --> (2) D-Glucose [C]

General reaction: O-Glycosyl bond hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8978084; 9403685 [H]