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The map label for this gene is rsmE2

Identifier: 15594703

GI number: 15594703

Start: 366229

End: 366960

Strand: Reverse

Name: rsmE2

Synonym: BB0358

Alternate gene names: 15594703

Gene position: 366960-366229 (Counterclockwise)

Preceding gene: 15594704

Following gene: 15594702

Centisome position: 40.29

GC content: 28.01

Gene sequence:

>732_bases
GTGAAGCAAATTGTTTTGGATGAGAATTGTTTAGCAGGTAATTTTATTATTGTTAAAGATGCAAAAATATATCACCATCT
TGTTAATGTAAGACGACTTAAAAAGGGTGATAAGCTGAACATTCTTTTAAAAGATAAAGAATTAAGGGCCTCAGAAATAG
TAAAGATTGGTAGTAATTTTATTAAGTTTACTACCAATAAAATAGATAAAATTGAAAAAAATAATTTTGAGATAAGTATT
TTTATTTCTAGTTTAAAGGGCAGAAAAATAGATTTGGTGTTAAGACAGGTTGTTGAGATTGGAGTTTCAGAAATCAATAT
TATTAATGCGGATCGTTCTGTGTCGAAAATAGATATAAACAATGCATCTGCCAAAATTTTAAGATTTTCAAAAATAATAG
ATGAGGCCTTAAAGCAAAGTGGTAATAAAATTGTTCCTAAAATTAATTTTTATAATAATTTTTTTTATTTACCTTATTCT
TTTTGTACTACCAGATATTATGTTGCTCATCCAAGTGGAATGATTTTAAGCAAGAATGAAAGTTTTGACAATTTTGGCAA
AATTGGAATTATAATAGGTCCTGAAGGATGCTTTTCGGAGTCCGAAATTGTCTTTTTCAAGGAGAAAGGCTTTAATTTTG
TAAGGTTTAACACTCCAATTTTACGAGCAGATACGGCTATTATTTATTCGCTTGCTTATTTTAAGGCATTGTTAGAGGAT
TATAATGGCTAA

Upstream 100 bases:

>100_bases
CTATCAGGACAATAATAAAGAATTGCCAATTTATAATCTACATTATGACAAAGTTTTAAAAACAGCTTGTGAGTATTTGT
CTAAATTAGGTAATTAAATT

Downstream 100 bases:

>100_bases
TTTAAAAGACATATATTCAAAACCAGACAGATTTTATTTTTTAGGTGTGCCTATAGATGTTTTTGATAGTCGCAGCAGCT
TATAAGCAGATTTGTCTATC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase 2

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKQIVLDENCLAGNFIIVKDAKIYHHLVNVRRLKKGDKLNILLKDKELRASEIVKIGSNFIKFTTNKIDKIEKNNFEISI
FISSLKGRKIDLVLRQVVEIGVSEINIINADRSVSKIDINNASAKILRFSKIIDEALKQSGNKIVPKINFYNNFFYLPYS
FCTTRYYVAHPSGMILSKNESFDNFGKIGIIIGPEGCFSESEIVFFKEKGFNFVRFNTPILRADTAIIYSLAYFKALLED
YNG

Sequences:

>Translated_243_residues
MKQIVLDENCLAGNFIIVKDAKIYHHLVNVRRLKKGDKLNILLKDKELRASEIVKIGSNFIKFTTNKIDKIEKNNFEISI
FISSLKGRKIDLVLRQVVEIGVSEINIINADRSVSKIDINNASAKILRFSKIIDEALKQSGNKIVPKINFYNNFFYLPYS
FCTTRYYVAHPSGMILSKNESFDNFGKIGIIIGPEGCFSESEIVFFKEKGFNFVRFNTPILRADTAIIYSLAYFKALLED
YNG
>Mature_243_residues
MKQIVLDENCLAGNFIIVKDAKIYHHLVNVRRLKKGDKLNILLKDKELRASEIVKIGSNFIKFTTNKIDKIEKNNFEISI
FISSLKGRKIDLVLRQVVEIGVSEINIINADRSVSKIDINNASAKILRFSKIIDEALKQSGNKIVPKINFYNNFFYLPYS
FCTTRYYVAHPSGMILSKNESFDNFGKIGIIIGPEGCFSESEIVFFKEKGFNFVRFNTPILRADTAIIYSLAYFKALLED
YNG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSME2_BORBU (O51333)

Other databases:

- EMBL:   AE000783
- PIR:   E70144
- RefSeq:   NP_212492.1
- ProteinModelPortal:   O51333
- EnsemblBacteria:   EBBORT00000009110
- GeneID:   1195195
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0358
- NMPDR:   fig|224326.1.peg.742
- TIGR:   BB_0358
- GeneTree:   EBGT00050000006712
- HOGENOM:   HBG664092
- OMA:   IGPEGCF
- ProtClustDB:   PRK11713
- BioCyc:   BBUR224326:BB_0358-MONOMER
- GO:   GO:0005737
- InterPro:   IPR015947
- InterPro:   IPR006700
- TIGRFAMs:   TIGR00046

Pfam domain/function: PF04452 Methyltrans_RNA; SSF88697 PUA-like

EC number: =2.1.1.193

Molecular weight: Translated: 27770; Mature: 27770

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIVLDENCLAGNFIIVKDAKIYHHLVNVRRLKKGDKLNILLKDKELRASEIVKIGSNF
CCEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCE
IKFTTNKIDKIEKNNFEISIFISSLKGRKIDLVLRQVVEIGVSEINIINADRSVSKIDIN
EEEECCHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEEC
NASAKILRFSKIIDEALKQSGNKIVPKINFYNNFFYLPYSFCTTRYYVAHPSGMILSKNE
CCHHHHHHHHHHHHHHHHCCCCEECEEEEEECCEEEEEEHHHHEEEEEECCCCEEEECCC
SFDNFGKIGIIIGPEGCFSESEIVFFKEKGFNFVRFNTPILRADTAIIYSLAYFKALLED
CCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECCCEEECCHHHHHHHHHHHHHHHH
YNG
CCC
>Mature Secondary Structure
MKQIVLDENCLAGNFIIVKDAKIYHHLVNVRRLKKGDKLNILLKDKELRASEIVKIGSNF
CCEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCE
IKFTTNKIDKIEKNNFEISIFISSLKGRKIDLVLRQVVEIGVSEINIINADRSVSKIDIN
EEEECCHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEEC
NASAKILRFSKIIDEALKQSGNKIVPKINFYNNFFYLPYSFCTTRYYVAHPSGMILSKNE
CCHHHHHHHHHHHHHHHHCCCCEECEEEEEECCEEEEEEHHHHEEEEEECCCCEEEECCC
SFDNFGKIGIIIGPEGCFSESEIVFFKEKGFNFVRFNTPILRADTAIIYSLAYFKALLED
CCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECCCEEECCHHHHHHHHHHHHHHHH
YNG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685