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The map label for this gene is flhF

Identifier: 15594615

GI number: 15594615

Start: 282699

End: 283865

Strand: Reverse

Name: flhF

Synonym: BB0270

Alternate gene names: 15594615

Gene position: 283865-282699 (Counterclockwise)

Preceding gene: 15594616

Following gene: 15594614

Centisome position: 31.17

GC content: 29.13

Gene sequence:

>1167_bases
ATGGTTCAATATTTTACAGAAAAAGGTCCAACTTATAATGAAGTCATAGAAATAATTAAGAAAAAATATGGTAAGAATGC
TAGGGTTATGACTTATAAAACCGTTCCCCATGGGGGGATTTTAGGTTTATTTAGCAAAGATTGGGTTGAAGTTTCAGGTT
ATGTTAGATATGACATTGGTAATCAACAAATTAATGTTGAAGATGAAAAGCGAAAGATTCTTCAAAGTATTAAAAGAGAA
GAAAATTCTTCAATTGAAGATGTGCTTAAAGAAGTTAAGTCTCTTAAAACAGAACTTGCTCATAAAAAAGAAAATATTAA
TCATCCAACAATTACAAAAATTGAAGACATTTTAAGAGAAAATGATTTTTCTGAAAATTATATTAAAGACATTAATGAGT
TTATTAAGAGAGAATTTAGTTTGTCAGATCTTGATGATTATGAAAGGGTTAGAGAGGATGTTGTTTTATATATTGCAAAG
ACAATTAAATGTTCAGGATCTATTATTGATAATCTTAAGAAAAGGGTTTTTATTTTAGTTGGTCCAACAGGTGTTGGAAA
AACTACCACAATTGCAAAACTTGCAGCAATTTATGGTATTAATGGAGAATCTAAGAGTTTAAATATTAAAATTATTACTA
TTGATAATTATCGTATTGGAGCTAAAAAACAAATACAAACTTATGGTGATATTATGGGTATTCCGGTTAGAGCAATTGAG
TCTTTTAAAGATTTAAAAGATGAAATTACTGATTCAAAGGATTTCGATCTTATACTTGTTGATACAATTGGTAAAAGTCC
CAAAGATTTCATGAAGCTTGCTGAGATGAAGGAGCTTCTTAATGCTTGTGGAAGGGATGCTGAATTTCATTTGGCTGTCA
GCTCTACCACAAAAACATCAGATGTCAAAGAAATATTTCACCAATTTTCTCCTTTTAATTATAAAACTGTGATTTTTACC
AAAGTGGATGAAACCACTTGTGTTGGAAATTTGATAAGTTTGATTTATGAAATGAAGAAGGTAGTTTCTTATGTTACGGA
TGGGCAAATTGTTCCTCATAATATCAGCGTTGCAGAACCACTTACTTTTATTAGAAGAATAAATGGCTACAGAATAAGCG
ATGATGCAGAGTTTATTAAGAAGATAAAAAGTAAATCTTATTATTAA

Upstream 100 bases:

>100_bases
AAGTAGAGAGATTCCAGATCTTGTTGTTATGTCTGTTTTAGAGGTTCCCCAAAATATTAAAGTTAATGTTCTTAAAACAG
TAGAGGTTGAAGAATAATAT

Downstream 100 bases:

>100_bases
GGAAAGTTATAATGGAAGATCAAGCTCAAAGTTTAAGAGATATGATGAGATTAAATGGTAAATTTAATTTTTCAGTTGAT
GAGAAAGTTCAAAACAGCAA

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MVQYFTEKGPTYNEVIEIIKKKYGKNARVMTYKTVPHGGILGLFSKDWVEVSGYVRYDIGNQQINVEDEKRKILQSIKRE
ENSSIEDVLKEVKSLKTELAHKKENINHPTITKIEDILRENDFSENYIKDINEFIKREFSLSDLDDYERVREDVVLYIAK
TIKCSGSIIDNLKKRVFILVGPTGVGKTTTIAKLAAIYGINGESKSLNIKIITIDNYRIGAKKQIQTYGDIMGIPVRAIE
SFKDLKDEITDSKDFDLILVDTIGKSPKDFMKLAEMKELLNACGRDAEFHLAVSSTTKTSDVKEIFHQFSPFNYKTVIFT
KVDETTCVGNLISLIYEMKKVVSYVTDGQIVPHNISVAEPLTFIRRINGYRISDDAEFIKKIKSKSYY

Sequences:

>Translated_388_residues
MVQYFTEKGPTYNEVIEIIKKKYGKNARVMTYKTVPHGGILGLFSKDWVEVSGYVRYDIGNQQINVEDEKRKILQSIKRE
ENSSIEDVLKEVKSLKTELAHKKENINHPTITKIEDILRENDFSENYIKDINEFIKREFSLSDLDDYERVREDVVLYIAK
TIKCSGSIIDNLKKRVFILVGPTGVGKTTTIAKLAAIYGINGESKSLNIKIITIDNYRIGAKKQIQTYGDIMGIPVRAIE
SFKDLKDEITDSKDFDLILVDTIGKSPKDFMKLAEMKELLNACGRDAEFHLAVSSTTKTSDVKEIFHQFSPFNYKTVIFT
KVDETTCVGNLISLIYEMKKVVSYVTDGQIVPHNISVAEPLTFIRRINGYRISDDAEFIKKIKSKSYY
>Mature_388_residues
MVQYFTEKGPTYNEVIEIIKKKYGKNARVMTYKTVPHGGILGLFSKDWVEVSGYVRYDIGNQQINVEDEKRKILQSIKRE
ENSSIEDVLKEVKSLKTELAHKKENINHPTITKIEDILRENDFSENYIKDINEFIKREFSLSDLDDYERVREDVVLYIAK
TIKCSGSIIDNLKKRVFILVGPTGVGKTTTIAKLAAIYGINGESKSLNIKIITIDNYRIGAKKQIQTYGDIMGIPVRAIE
SFKDLKDEITDSKDFDLILVDTIGKSPKDFMKLAEMKELLNACGRDAEFHLAVSSTTKTSDVKEIFHQFSPFNYKTVIFT
KVDETTCVGNLISLIYEMKKVVSYVTDGQIVPHNISVAEPLTFIRRINGYRISDDAEFIKKIKSKSYY

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FLHF_BORBU (Q44758)

Other databases:

- EMBL:   U43739
- EMBL:   L75945
- EMBL:   AE000783
- PIR:   F70133
- RefSeq:   NP_212404.1
- ProteinModelPortal:   Q44758
- SMR:   Q44758
- EnsemblBacteria:   EBBORT00000008966
- GeneID:   1195107
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0270
- NMPDR:   fig|224326.1.peg.654
- TIGR:   BB_0270
- GeneTree:   EBGT00050000007189
- HOGENOM:   HBG732082
- OMA:   TENIFRP
- ProtClustDB:   PRK12723
- BioCyc:   BBUR224326:BB_0270-MONOMER
- InterPro:   IPR003593
- InterPro:   IPR000897
- InterPro:   IPR020006
- SMART:   SM00382
- SMART:   SM00962
- TIGRFAMs:   TIGR03499

Pfam domain/function: PF00448 SRP54

EC number: NA

Molecular weight: Translated: 44370; Mature: 44370

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQYFTEKGPTYNEVIEIIKKKYGKNARVMTYKTVPHGGILGLFSKDWVEVSGYVRYDIG
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCEEECCEEEEECC
NQQINVEDEKRKILQSIKREENSSIEDVLKEVKSLKTELAHKKENINHPTITKIEDILRE
CCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NDFSENYIKDINEFIKREFSLSDLDDYERVREDVVLYIAKTIKCSGSIIDNLKKRVFILV
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEE
GPTGVGKTTTIAKLAAIYGINGESKSLNIKIITIDNYRIGAKKQIQTYGDIMGIPVRAIE
CCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCEECCCHHHHHHHHHHHCCCHHHHH
SFKDLKDEITDSKDFDLILVDTIGKSPKDFMKLAEMKELLNACGRDAEFHLAVSSTTKTS
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHH
DVKEIFHQFSPFNYKTVIFTKVDETTCVGNLISLIYEMKKVVSYVTDGQIVPHNISVAEP
HHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHH
LTFIRRINGYRISDDAEFIKKIKSKSYY
HHHHHHHCCCEECCHHHHHHHHHHCCCC
>Mature Secondary Structure
MVQYFTEKGPTYNEVIEIIKKKYGKNARVMTYKTVPHGGILGLFSKDWVEVSGYVRYDIG
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCEEECCEEEEECC
NQQINVEDEKRKILQSIKREENSSIEDVLKEVKSLKTELAHKKENINHPTITKIEDILRE
CCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NDFSENYIKDINEFIKREFSLSDLDDYERVREDVVLYIAKTIKCSGSIIDNLKKRVFILV
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEE
GPTGVGKTTTIAKLAAIYGINGESKSLNIKIITIDNYRIGAKKQIQTYGDIMGIPVRAIE
CCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCEECCCHHHHHHHHHHHCCCHHHHH
SFKDLKDEITDSKDFDLILVDTIGKSPKDFMKLAEMKELLNACGRDAEFHLAVSSTTKTS
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHH
DVKEIFHQFSPFNYKTVIFTKVDETTCVGNLISLIYEMKKVVSYVTDGQIVPHNISVAEP
HHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHH
LTFIRRINGYRISDDAEFIKKIKSKSYY
HHHHHHHCCCEECCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9168127; 9403685