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The map label for this gene is rho

Identifier: 15594575

GI number: 15594575

Start: 235595

End: 237142

Strand: Reverse

Name: rho

Synonym: BB0230

Alternate gene names: 15594575

Gene position: 237142-235595 (Counterclockwise)

Preceding gene: 15594576

Following gene: 15594574

Centisome position: 26.04

GC content: 31.98

Gene sequence:

>1548_bases
ATGGATAAAAAAAATGGTGGATTTGATTTAGAAAGTGAAATAAGGCGGTTGGATGTTTCTAAAGAGTTTAAGATTGAAGA
TAATTTGAAAAAAAAAGTGGTTAAAGTTGTTGCTAAAAAGGATTCTGCATCTAGCGTGACAAAATCTGCAGATTTAAGTG
GCGTAAAGGATTCAAACGGGGTAATATTTTCTGATTTTGATTATGATATTCCTTCTTCAGGTTTAGAAAATAATATTAAA
ACTTTAGAGCAAAGTAATATCATCAAATTTTTTAATGGTAAAGATTATGTAGAGATTGAAAAACTTTATGATAAGCCAAT
TACAGAGGTTAGAAAAATTGTTGAAGGTCTTGGGACCAATCATACTATTGCTGTAACAATGAAAAAAACCGAATTAATAT
TTTTATTGGTGAAAATATTAAGTGAGAATAATATTGATGTTTTATTTACAGGTGTGCTTGATGTGCTTAGTGATGGTTAT
GGGTTTTTGAGAACTGCATCAAATTCTTATCTTTCTGGAGGCAATGATGTTTACGTTTCTCCCTCTCAGATTAGACTTTT
TAATTTAAGGACAGGTGATATTTTATATGGCCAAATTAGATCGCCTAGAGATGGTGAGAGATTTTTTGCAATGGTTAAAA
TTAAATCTATTAATGATCAAGATCCTACGTTTGCTCAAAACAGAATACCTTTTGATAATTTAACTCCTCTTTATCCCAAT
GTGAAATTGAATCTTGAATATGAAAATTGCAATATTTCTACAAGGCTTATTAATCTTTTTTCACCTATAGGTAAGGGGCA
AAGGGCTTTAATAGTTTCTCCTCCAAAAGCGGGAAAGACTACCCTGCTTCAAAAAATAGCTAATGCAATAACTACTAATT
ATTCAGATGTTATTTTAATGATACTACTTATTGATGAGAGGCCAGAAGAAGTTACAGATATGATTCGTAGCGTTAAAGGC
GAAGTAATTGCATCTAATTTTGATGAGCAGGCTAGTAGGCATGTTCAGGTAGCAGAGATGGTTATTGAAAAGGCTAAAAG
GCTTGTTGAAAACAAAAAAGATGTTGTTATTTTGCTTGATTCTATTACAAGGCTTGCAAGGGCATATAATCAAACTATGC
CAACTTCTGGTAAAATTTTATCGGGTGGGGTAGATTCTAATGCTCTTCATAAGCCAAAGAGGTTTTTTGGGTCTGCTAGA
AATATAGAAGAAGGGGGAAGTTTAACTATTATAGCTACTGCTTTGGTTGATACTGGTAGTAAAATGGATGAAGTTATTTT
TGAAGAATTTAAAAGTACCGGTAATATGGAATTAATTCTTGATAGAAGTTTGGCAGACAGAAGACTTTTTCCTGCTATTA
ATATTAAAAAGTCAGGTACCAGAAAAGAAGAATTGCTTCTTAGTGAAGAGGAGCGTTCTAAGATTTTGCTTATTAGAAGA
ATACTTGGAGGTGTTGATGATTATGAGGGAGTTGAAGTTCTGATAGAAAAGATGAAAAAAAGCAAAAACAATGAAATTTT
CTTGAAGACAATGAGCAATGGTAATTGA

Upstream 100 bases:

>100_bases
ATAAAAGATACTTTGCAATAGGGTCAGTGTTTTTTATTAATTAATAATAAGTAGATTTTTATAAAATCCTTTCTTAAAAA
TTTAATTCGTAGAGGAAAAT

Downstream 100 bases:

>100_bases
ATAAATTGACATTATTTCTAATGTAAATTAAACTTTATTGAAGATTAAGAGGAAGGTTTTATGAGAAAAGATATACATCC
TAAAAATAATTTAGTGGTAT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MDKKNGGFDLESEIRRLDVSKEFKIEDNLKKKVVKVVAKKDSASSVTKSADLSGVKDSNGVIFSDFDYDIPSSGLENNIK
TLEQSNIIKFFNGKDYVEIEKLYDKPITEVRKIVEGLGTNHTIAVTMKKTELIFLLVKILSENNIDVLFTGVLDVLSDGY
GFLRTASNSYLSGGNDVYVSPSQIRLFNLRTGDILYGQIRSPRDGERFFAMVKIKSINDQDPTFAQNRIPFDNLTPLYPN
VKLNLEYENCNISTRLINLFSPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYSDVILMILLIDERPEEVTDMIRSVKG
EVIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLDSITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSAR
NIEEGGSLTIIATALVDTGSKMDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRR
ILGGVDDYEGVEVLIEKMKKSKNNEIFLKTMSNGN

Sequences:

>Translated_515_residues
MDKKNGGFDLESEIRRLDVSKEFKIEDNLKKKVVKVVAKKDSASSVTKSADLSGVKDSNGVIFSDFDYDIPSSGLENNIK
TLEQSNIIKFFNGKDYVEIEKLYDKPITEVRKIVEGLGTNHTIAVTMKKTELIFLLVKILSENNIDVLFTGVLDVLSDGY
GFLRTASNSYLSGGNDVYVSPSQIRLFNLRTGDILYGQIRSPRDGERFFAMVKIKSINDQDPTFAQNRIPFDNLTPLYPN
VKLNLEYENCNISTRLINLFSPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYSDVILMILLIDERPEEVTDMIRSVKG
EVIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLDSITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSAR
NIEEGGSLTIIATALVDTGSKMDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRR
ILGGVDDYEGVEVLIEKMKKSKNNEIFLKTMSNGN
>Mature_515_residues
MDKKNGGFDLESEIRRLDVSKEFKIEDNLKKKVVKVVAKKDSASSVTKSADLSGVKDSNGVIFSDFDYDIPSSGLENNIK
TLEQSNIIKFFNGKDYVEIEKLYDKPITEVRKIVEGLGTNHTIAVTMKKTELIFLLVKILSENNIDVLFTGVLDVLSDGY
GFLRTASNSYLSGGNDVYVSPSQIRLFNLRTGDILYGQIRSPRDGERFFAMVKIKSINDQDPTFAQNRIPFDNLTPLYPN
VKLNLEYENCNISTRLINLFSPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYSDVILMILLIDERPEEVTDMIRSVKG
EVIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLDSITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSAR
NIEEGGSLTIIATALVDTGSKMDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRR
ILGGVDDYEGVEVLIEKMKKSKNNEIFLKTMSNGN

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=58.1730769230769, Blast_Score=504, Evalue=1e-144,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RHO_BORBU (P33561)

Other databases:

- EMBL:   L07656
- EMBL:   U35673
- EMBL:   AE000783
- EMBL:   L46347
- PIR:   F70128
- RefSeq:   NP_212364.1
- ProteinModelPortal:   P33561
- SMR:   P33561
- EnsemblBacteria:   EBBORT00000008833
- GeneID:   1195067
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0230
- NMPDR:   fig|224326.1.peg.614
- TIGR:   BB_0230
- GeneTree:   EBGT00050000006809
- HOGENOM:   HBG335262
- OMA:   YNYLPGP
- PhylomeDB:   P33561
- ProtClustDB:   PRK09376
- BioCyc:   BBUR224326:BB_0230-MONOMER
- HAMAP:   MF_01884
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011113
- InterPro:   IPR004665
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00382
- SMART:   SM00357
- TIGRFAMs:   TIGR00767

Pfam domain/function: PF00006 ATP-synt_ab; PF07497 Rho_RNA_bind; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 57626; Mature: 57626

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKNGGFDLESEIRRLDVSKEFKIEDNLKKKVVKVVAKKDSASSVTKSADLSGVKDSNG
CCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCC
VIFSDFDYDIPSSGLENNIKTLEQSNIIKFFNGKDYVEIEKLYDKPITEVRKIVEGLGTN
EEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEHHHHHCCCHHHHHHHHHHCCCC
HTIAVTMKKTELIFLLVKILSENNIDVLFTGVLDVLSDGYGFLRTASNSYLSGGNDVYVS
CEEEEEEEHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEC
PSQIRLFNLRTGDILYGQIRSPRDGERFFAMVKIKSINDQDPTFAQNRIPFDNLTPLYPN
CCEEEEEEECCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
VKLNLEYENCNISTRLINLFSPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYSDVILM
EEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHEEE
ILLIDERPEEVTDMIRSVKGEVIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLD
EEEECCCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHH
SITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSARNIEEGGSLTIIATALVDTGS
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEEECCCCC
KMDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRR
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHCHHHHHHHHHHHH
ILGGVDDYEGVEVLIEKMKKSKNNEIFLKTMSNGN
HHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCC
>Mature Secondary Structure
MDKKNGGFDLESEIRRLDVSKEFKIEDNLKKKVVKVVAKKDSASSVTKSADLSGVKDSNG
CCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCC
VIFSDFDYDIPSSGLENNIKTLEQSNIIKFFNGKDYVEIEKLYDKPITEVRKIVEGLGTN
EEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEHHHHHCCCHHHHHHHHHHCCCC
HTIAVTMKKTELIFLLVKILSENNIDVLFTGVLDVLSDGYGFLRTASNSYLSGGNDVYVS
CEEEEEEEHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEC
PSQIRLFNLRTGDILYGQIRSPRDGERFFAMVKIKSINDQDPTFAQNRIPFDNLTPLYPN
CCEEEEEEECCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
VKLNLEYENCNISTRLINLFSPIGKGQRALIVSPPKAGKTTLLQKIANAITTNYSDVILM
EEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHEEE
ILLIDERPEEVTDMIRSVKGEVIASNFDEQASRHVQVAEMVIEKAKRLVENKKDVVILLD
EEEECCCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHH
SITRLARAYNQTMPTSGKILSGGVDSNALHKPKRFFGSARNIEEGGSLTIIATALVDTGS
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEEECCCCC
KMDEVIFEEFKSTGNMELILDRSLADRRLFPAINIKKSGTRKEELLLSEEERSKILLIRR
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHCHHHHHHHHHHHH
ILGGVDDYEGVEVLIEKMKKSKNNEIFLKTMSNGN
HHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8451174; 9403685; 7812434