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The map label for this gene is serS [H]

Identifier: 15594571

GI number: 15594571

Start: 230214

End: 231491

Strand: Reverse

Name: serS [H]

Synonym: BB0226

Alternate gene names: 15594571

Gene position: 231491-230214 (Counterclockwise)

Preceding gene: 15594574

Following gene: 15594569

Centisome position: 25.42

GC content: 31.22

Gene sequence:

>1278_bases
ATGCTTGATCTGAAATTTATTAGAGATAACCTTGATCTTGTTAAGAGAAGTATTAAAGCAAGAGGCCTTGTTTTAGATAT
TGATAAGTTAATTTATCTTGATGATAAGAGAAAAAAACTAATTACCAAAATTGGTGAGCTTAATGCAAAAAGAAATGAAA
ATTCTTCTAAAATGCAAGAAAATCTTGATAAAGTTTTAAAAATCTCTTTAATAGAAACCGGAAAGATTTTGAAAAAACAG
CTTATTGATCTAGAAGAAGAGCTTGAAAGAGTATCTGTTGATTTTGACCTTGAAAATAAGAAAGTGCCCAATATTTTGTC
ACCTGATGTGCCTATTGGCAGTAGTGAAGAAGATAATTTTGAAATAAAAAGGGTTGGAGTTGTTCCACAGTTTGATTTTA
AGCCAAAAGATCATTTAGAGCTTGGCAGAGATTTAGATCTTCTAGATTTTGACAGAGCACGTGAGATTAGCGGGAGTAAA
TTTTATTATCTTAAAAATGAAGCAGTTTTTTTAGAAATTGCTCTTATTAATTTCTCTTTAAATAAATTAAGAGATAAAGG
TTTTGATGTTTTTATTACTCCTGATGTTGCAAGAGAGTTTATAGTTGATGGAATAGGTTTTAATCCTCGTGGCAACGAAA
GCAATATTTATAAGATTGAAGATACAGATAAATATCTTGTGGGCACTTCTGAAATTACGCTTGGCGGTTATTATTATAAT
AAAATAATAGATCTCACTTTGCCAATAAGAATGGCAGGGTTTTCACATTGTTTTCGAAAAGAAGCTGGAGCATATGGTCA
GCTTTCAAAAGGTCTTTATAGAGTTCATCAGTTTAGCAAGGTGGAGATGTTTTGTTTTTGCAAAGCAGAAGAATCTGGAG
TTATTCATGATGAATTTTTAAGTATTCAAGAGCAAATTTTTACTGAGCTTGAAATTCCTTATAGGGTTTTAAATATTTGT
TCTTTTGATCTTGGTTCTCCAGCTTATAAAAAATATGATATTGAAGCTTGGATGCCTGGAAGAGACGGAAAAGGTGGTTA
TGGGGAGGTTACTTCAACTTCAAATTGTACAGATTATCAGTCAAGAAGGCTTAAGATTAGATACAAAGATCAAGATGGAC
AAAATAAATTTGCACACATGGTAAATGGAACAGCAATAGCTACAACAAGGGTTATTATTTCTATACTTGAAAACTTTCAG
GATCAAAAGGGAGGAGTTAGAATTCCTAAAAGTTTGGTTAAGTATACAGGCTTTGATTATATTCCTTTTAAGAATTAG

Upstream 100 bases:

>100_bases
TAATCCTGTTATAAGATATTATATAATAACCTTAATATGAATTAATATTATTTGTAATTTTGTTTAAAAATTTTACTTAA
GACATATCTTAGGAGATAGG

Downstream 100 bases:

>100_bases
AATTTAGTATTTTTTCTGGCAATGTTTCTATAGCTTTTAGCAATATTTCATGCTTTTTTAAGTTTTTTGGAAATGGTGCT
GTTAAATTTTGTCTAAATTT

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLDLKFIRDNLDLVKRSIKARGLVLDIDKLIYLDDKRKKLITKIGELNAKRNENSSKMQENLDKVLKISLIETGKILKKQ
LIDLEEELERVSVDFDLENKKVPNILSPDVPIGSSEEDNFEIKRVGVVPQFDFKPKDHLELGRDLDLLDFDRAREISGSK
FYYLKNEAVFLEIALINFSLNKLRDKGFDVFITPDVAREFIVDGIGFNPRGNESNIYKIEDTDKYLVGTSEITLGGYYYN
KIIDLTLPIRMAGFSHCFRKEAGAYGQLSKGLYRVHQFSKVEMFCFCKAEESGVIHDEFLSIQEQIFTELEIPYRVLNIC
SFDLGSPAYKKYDIEAWMPGRDGKGGYGEVTSTSNCTDYQSRRLKIRYKDQDGQNKFAHMVNGTAIATTRVIISILENFQ
DQKGGVRIPKSLVKYTGFDYIPFKN

Sequences:

>Translated_425_residues
MLDLKFIRDNLDLVKRSIKARGLVLDIDKLIYLDDKRKKLITKIGELNAKRNENSSKMQENLDKVLKISLIETGKILKKQ
LIDLEEELERVSVDFDLENKKVPNILSPDVPIGSSEEDNFEIKRVGVVPQFDFKPKDHLELGRDLDLLDFDRAREISGSK
FYYLKNEAVFLEIALINFSLNKLRDKGFDVFITPDVAREFIVDGIGFNPRGNESNIYKIEDTDKYLVGTSEITLGGYYYN
KIIDLTLPIRMAGFSHCFRKEAGAYGQLSKGLYRVHQFSKVEMFCFCKAEESGVIHDEFLSIQEQIFTELEIPYRVLNIC
SFDLGSPAYKKYDIEAWMPGRDGKGGYGEVTSTSNCTDYQSRRLKIRYKDQDGQNKFAHMVNGTAIATTRVIISILENFQ
DQKGGVRIPKSLVKYTGFDYIPFKN
>Mature_425_residues
MLDLKFIRDNLDLVKRSIKARGLVLDIDKLIYLDDKRKKLITKIGELNAKRNENSSKMQENLDKVLKISLIETGKILKKQ
LIDLEEELERVSVDFDLENKKVPNILSPDVPIGSSEEDNFEIKRVGVVPQFDFKPKDHLELGRDLDLLDFDRAREISGSK
FYYLKNEAVFLEIALINFSLNKLRDKGFDVFITPDVAREFIVDGIGFNPRGNESNIYKIEDTDKYLVGTSEITLGGYYYN
KIIDLTLPIRMAGFSHCFRKEAGAYGQLSKGLYRVHQFSKVEMFCFCKAEESGVIHDEFLSIQEQIFTELEIPYRVLNIC
SFDLGSPAYKKYDIEAWMPGRDGKGGYGEVTSTSNCTDYQSRRLKIRYKDQDGQNKFAHMVNGTAIATTRVIISILENFQ
DQKGGVRIPKSLVKYTGFDYIPFKN

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) [H]

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily [H]

Homologues:

Organism=Homo sapiens, GI8923421, Length=400, Percent_Identity=39.25, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI225579074, Length=344, Percent_Identity=43.3139534883721, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI16306548, Length=384, Percent_Identity=35.15625, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1787120, Length=438, Percent_Identity=32.648401826484, Blast_Score=236, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17539260, Length=446, Percent_Identity=33.4080717488789, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17542084, Length=421, Percent_Identity=33.729216152019, Blast_Score=194, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6321799, Length=333, Percent_Identity=41.1411411411411, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6320226, Length=455, Percent_Identity=33.8461538461538, Blast_Score=209, Evalue=8e-55,
Organism=Drosophila melanogaster, GI17737985, Length=322, Percent_Identity=38.5093167701863, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24581334, Length=377, Percent_Identity=33.6870026525199, Blast_Score=191, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.11 [H]

Molecular weight: Translated: 48788; Mature: 48788

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLKFIRDNLDLVKRSIKARGLVLDIDKLIYLDDKRKKLITKIGELNAKRNENSSKMQE
CCCHHHHHCCHHHHHHHHHHCCEEEEECCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHH
NLDKVLKISLIETGKILKKQLIDLEEELERVSVDFDLENKKVPNILSPDVPIGSSEEDNF
HHHHHHEEHHHHHHHHHHHHHHCHHHHHHHEEECEECCCCCCCCCCCCCCCCCCCCCCCE
EIKRVGVVPQFDFKPKDHLELGRDLDLLDFDRAREISGSKFYYLKNEAVFLEIALINFSL
EEEEEECCCCCCCCCCHHHHCCCCCCCCCCCHHHCCCCCEEEEEECCEEEEEEEEEECCH
NKLRDKGFDVFITPDVAREFIVDGIGFNPRGNESNIYKIEDTDKYLVGTSEITLGGYYYN
HHHHCCCCEEEECHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEEECCEEEECEEEEE
KIIDLTLPIRMAGFSHCFRKEAGAYGQLSKGLYRVHQFSKVEMFCFCKAEESGVIHDEFL
EEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHH
SIQEQIFTELEIPYRVLNICSFDLGSPAYKKYDIEAWMPGRDGKGGYGEVTSTSNCTDYQ
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
SRRLKIRYKDQDGQNKFAHMVNGTAIATTRVIISILENFQDQKGGVRIPKSLVKYTGFDY
CCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCCCCC
IPFKN
CCCCC
>Mature Secondary Structure
MLDLKFIRDNLDLVKRSIKARGLVLDIDKLIYLDDKRKKLITKIGELNAKRNENSSKMQE
CCCHHHHHCCHHHHHHHHHHCCEEEEECCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHH
NLDKVLKISLIETGKILKKQLIDLEEELERVSVDFDLENKKVPNILSPDVPIGSSEEDNF
HHHHHHEEHHHHHHHHHHHHHHCHHHHHHHEEECEECCCCCCCCCCCCCCCCCCCCCCCE
EIKRVGVVPQFDFKPKDHLELGRDLDLLDFDRAREISGSKFYYLKNEAVFLEIALINFSL
EEEEEECCCCCCCCCCHHHHCCCCCCCCCCCHHHCCCCCEEEEEECCEEEEEEEEEECCH
NKLRDKGFDVFITPDVAREFIVDGIGFNPRGNESNIYKIEDTDKYLVGTSEITLGGYYYN
HHHHCCCCEEEECHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEEECCEEEECEEEEE
KIIDLTLPIRMAGFSHCFRKEAGAYGQLSKGLYRVHQFSKVEMFCFCKAEESGVIHDEFL
EEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHH
SIQEQIFTELEIPYRVLNICSFDLGSPAYKKYDIEAWMPGRDGKGGYGEVTSTSNCTDYQ
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
SRRLKIRYKDQDGQNKFAHMVNGTAIATTRVIISILENFQDQKGGVRIPKSLVKYTGFDY
CCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCCCCC
IPFKN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA