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The map label for this gene is dus2 [H]

Identifier: 15594570

GI number: 15594570

Start: 229217

End: 230224

Strand: Direct

Name: dus2 [H]

Synonym: BB0225

Alternate gene names: 15594570

Gene position: 229217-230224 (Clockwise)

Preceding gene: 15594563

Following gene: 15594572

Centisome position: 25.17

GC content: 28.17

Gene sequence:

>1008_bases
ATGTTAATGAATCGAAAAATATCAATAGCCCCAATGGTAAACATTACAGACGAGCATTTCAGATATTTAATAAGACTTTT
ATCAAAAAAAGTTACATTATATACCCCAATGATTTCTGCAAAGTCAATAATTATGGGTGATGTAAAAAAAATTGTAAAGC
AAAACCCCTTAGAATCTCCAATTGCAATTCAAATAGCAACAAACTCCAAAAATGATGCCCTTAAAGCTATTCAAATTCTT
GAAAAACACTTTAATTTTGACGAATACAATCTTAATGTTGGATGCCCATCTCTAAAAATCCAAAATGCAAATTGTGGAGC
TTGTCTAATGGGCAATGCTAATCAAGTAGGTGAAATTTTAAAAACGATGAAAGAAAACACAAATAAGCCTATTTCAATTA
AACATAGATTGGGTATAAGGCCTTTATCTAGTGATTACAAAAATGAAAGTTATTCTGAGGTAAAAAACTTTGTCAAAATA
ATCTCAGATTTTGGTATCAAAAATTTTATTGTACATGCAAGGATTGCAATATTAAAAGGATTTTCTCCTAAAAATAATAG
GAATATTCCAAAATTAAGACATGAATTTGTATATAAGCTGAAAAAAGAGAATAAAAATTTGTTTATCGAGATAAATGGGG
GAATCAATAACTCAGAGCAAATTAAAAAACACCTATCTTTTGTTGATTCTGTCATGATTGGGAGAAGTGCGTTTGAAAAT
CCATACTTTATTGCAAACGCCTCAAGAGAATTTTTAAACGAAACTGATGAAATTCTTACAAGAAAAGATATATTGATGCA
AATGGTAGAATACATTAAAGAATACGAAAATTATTTGTCGATCAATATATTATTCAAGCATCTTATGGGAATAGTATTCT
CCAAAGAAGGAGCTAAAAAATTTAGACAAAATTTAACAGCACCATTTCCAAAAAACTTAAAAAAGCATGAAATATTGCTA
AAAGCTATAGAAACATTGCCAGAAAAAATACTAAATTCTAATTCTTAA

Upstream 100 bases:

>100_bases
ATTAAAAAAAATAAATCCTTTCTTCCAGGCATATAACAATTCCTCCTAAAAAAGAATAAATAATTGTTAAATCAGCTCGT
TTATTCTAACATATTATATA

Downstream 100 bases:

>100_bases
AAGGAATATAATCAAAGCCTGTATACTTAACCAAACTTTTAGGAATTCTAACTCCTCCCTTTTGATCCTGAAAGTTTTCA
AGTATAGAAATAATAACCCT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MLMNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKIVKQNPLESPIAIQIATNSKNDALKAIQIL
EKHFNFDEYNLNVGCPSLKIQNANCGACLMGNANQVGEILKTMKENTNKPISIKHRLGIRPLSSDYKNESYSEVKNFVKI
ISDFGIKNFIVHARIAILKGFSPKNNRNIPKLRHEFVYKLKKENKNLFIEINGGINNSEQIKKHLSFVDSVMIGRSAFEN
PYFIANASREFLNETDEILTRKDILMQMVEYIKEYENYLSINILFKHLMGIVFSKEGAKKFRQNLTAPFPKNLKKHEILL
KAIETLPEKILNSNS

Sequences:

>Translated_335_residues
MLMNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKIVKQNPLESPIAIQIATNSKNDALKAIQIL
EKHFNFDEYNLNVGCPSLKIQNANCGACLMGNANQVGEILKTMKENTNKPISIKHRLGIRPLSSDYKNESYSEVKNFVKI
ISDFGIKNFIVHARIAILKGFSPKNNRNIPKLRHEFVYKLKKENKNLFIEINGGINNSEQIKKHLSFVDSVMIGRSAFEN
PYFIANASREFLNETDEILTRKDILMQMVEYIKEYENYLSINILFKHLMGIVFSKEGAKKFRQNLTAPFPKNLKKHEILL
KAIETLPEKILNSNS
>Mature_335_residues
MLMNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKIVKQNPLESPIAIQIATNSKNDALKAIQIL
EKHFNFDEYNLNVGCPSLKIQNANCGACLMGNANQVGEILKTMKENTNKPISIKHRLGIRPLSSDYKNESYSEVKNFVKI
ISDFGIKNFIVHARIAILKGFSPKNNRNIPKLRHEFVYKLKKENKNLFIEINGGINNSEQIKKHLSFVDSVMIGRSAFEN
PYFIANASREFLNETDEILTRKDILMQMVEYIKEYENYLSINILFKHLMGIVFSKEGAKKFRQNLTAPFPKNLKKHEILL
KAIETLPEKILNSNS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Escherichia coli, GI145693211, Length=303, Percent_Identity=37.2937293729373, Blast_Score=215, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38434; Mature: 38434

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKIVKQNPLESP
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHCCCCCCC
IAIQIATNSKNDALKAIQILEKHFNFDEYNLNVGCPSLKIQNANCGACLMGNANQVGEIL
EEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCEEEECCCHHHHHHH
KTMKENTNKPISIKHRLGIRPLSSDYKNESYSEVKNFVKIISDFGIKNFIVHARIAILKG
HHHHHCCCCCEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
FSPKNNRNIPKLRHEFVYKLKKENKNLFIEINGGINNSEQIKKHLSFVDSVMIGRSAFEN
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PYFIANASREFLNETDEILTRKDILMQMVEYIKEYENYLSINILFKHLMGIVFSKEGAKK
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FRQNLTAPFPKNLKKHEILLKAIETLPEKILNSNS
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLMNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKIVKQNPLESP
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHCCCCCCC
IAIQIATNSKNDALKAIQILEKHFNFDEYNLNVGCPSLKIQNANCGACLMGNANQVGEIL
EEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCEEEECCCHHHHHHH
KTMKENTNKPISIKHRLGIRPLSSDYKNESYSEVKNFVKIISDFGIKNFIVHARIAILKG
HHHHHCCCCCEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
FSPKNNRNIPKLRHEFVYKLKKENKNLFIEINGGINNSEQIKKHLSFVDSVMIGRSAFEN
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PYFIANASREFLNETDEILTRKDILMQMVEYIKEYENYLSINILFKHLMGIVFSKEGAKK
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FRQNLTAPFPKNLKKHEILLKAIETLPEKILNSNS
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]