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The map label for this gene is spoT

Identifier: 15594543

GI number: 15594543

Start: 195693

End: 197696

Strand: Direct

Name: spoT

Synonym: BB0198

Alternate gene names: 15594543

Gene position: 195693-197696 (Clockwise)

Preceding gene: 15594542

Following gene: 15594544

Centisome position: 21.49

GC content: 29.94

Gene sequence:

>2004_bases
ATGATACAAGCATACGAAATTGCACATTTAATCAAGATAAATGATCTTGAAAAAGCTAGAAATATTTTTAAAAAAACTGT
TGAAAATACTTACAAAGATGAATTTGAACGAAAAAGCATATTCAAAGCTCTAGAAATAGCAGAACAGCTCCACTATGGCC
AATATAGAGAAAGTGGAGAGCCTTACATTATTCACCCAATAATGGTTTCATTATTTCTTGCCAAATTTCAACTAGATTTT
AAAGCAACTATTGCTGGACTACTGCATGACGTACTTGAAGATACAAATGTTGAAAAAGAAGAAATAGTAAAAGAATTTGA
CGAGGAAATTTTAAGCCTAATCGACGGTGTAACTAAAATTCATGATTTACACAATAAAACAAGAAGCATAAAAGAGGCTA
ATACTATCTCAAAAATGTTTTTTGCAATGACACATGATATTAGAATAATAATAATAAAACTGGCAGATAAACTTCATAAT
ATGACAACTCTCTCTTATTTGCCCAAAAACAGACAAGACAGGATTGCAAAAGATTGCCTTTCAACTTACGTTCCAATAGC
AGAACGCCTTGGGATCTCATCTCTTAAAACATATCTTGAGGATCTTTCATTTAAGCATCTTTATCCTAAAGATTATAAAG
AAATAAAAAATTTTTTATCTGAAACAAAAATAGAAAGAGAAAAAAAATTATACAAAGGAAAATTATCAATAGAAAAAGAA
CTTCAAAAAAGCGGAATTGAAGCAGAAATTACAGTAAGATCAAAACACTTTTATTCAATATTTAGAAAAATGCAAACAAG
AACAAACAAACTTACTCAAATTTTTGATACTCTAGGAATAAGAATAATTTGCAAAAAACAAAAAGAATGTTATGAGATAT
TAGAAATTGTTCACCGGGTGTGGAAACCAATCCCGGGAAGACTTAAAGACTATATTGCAAGCCCAAAAGAAAATAAATAT
CAATCACTACACACTACTGTAAGAATACCTGAGGATAACCAGCTTATTGAAATACAAATTCGAACAGAAGAAATGGATAG
AATTGCCAATTATGGGGTTGCAGCCCACTGGATATATAAAGAACAAATTGAACTTAAAGCTGATGATCTTTCATTTATAA
ACCGAATAAAAAAATGGCAACAAGAATCGGCCAACAAAAGTCAATATTCAATGAATGACATACACAAAGAGTTGTTGAAT
ACATTCATATATGTTTACACCCCAGAAGGAGAAGTAGTAGAACTTCCTTTTGGATCAAATTCAATTGATTTTGCATACAT
AATTCACACAGATATTGGAGATCAAGCTCTTTATGCAAAAATAAACGGAAAAATAAGCTCAATCACAAAACCGCTTAAAA
ACGAACAAATTGTTGAAATATTCACATCAAAAGACTCAAAACCGGATGTAATATGGCTAAACAGCGTAAGAACAAAAAAG
GCCCGATCAAAAATTAGATCATGGCTTAACAAGAATGACAACACAATTTTTGTAGACAACAATATTATTGCTTATCTTGT
TGGAGCAAACAAAGAGCAAAGAAAGCTTTTCAGCTTATTCAAATCTTATACCAAAACCAAAATAAAAAGAATTGCAATAG
ACCCTGAATGTAGTCCAACAACAGGTGAAGATATTATTGGAATAATACATAAAGACGAAATAATAGTGCACAATGAAAAT
TGTCAAAAGCTTAAATCCTATAAAAAACCCCAATTGATTGAAGTTGAGTGGGAAGCAACACCAACTAGAAAAGTGCATCA
CATTATTTTACTTTTAAAAGAGCTTAAAGGAATATTTAGCTATCTTGAAAACATTTTCACACTCAATGACGTAAGACTTA
TTAGCGAAAAAATAGAAGACTGCGGAAATGGTCATGGAATAACAAATATAATAGTCTCATCAAATGCTAAAAATATAACA
AAAATTATTTCTGCTTTAAAAGAAAACCCAAATATCTTACAAATAATGCAAATAGAAGAAGACATTAAAAATTATGACAA
TTAA

Upstream 100 bases:

>100_bases
AAAAGCTTGAAAGATTTTGCAATCAAAAAGGGATTTTCACATTTAAAAACAATTTACGATCTTGAAAAGAGAGCAAGGGC
GCTGATATTGGGACAAAGAG

Downstream 100 bases:

>100_bases
TATATTGTTATTATTGTTATTGCTTAGCTCAACAGGCCCCACCATTGTGCCAAATAAAATGGAAAAAAATGCATTCAATT
CAAGCAATAAAATTACAAGC

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MIQAYEIAHLIKINDLEKARNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGEPYIIHPIMVSLFLAKFQLDF
KATIAGLLHDVLEDTNVEKEEIVKEFDEEILSLIDGVTKIHDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHN
MTTLSYLPKNRQDRIAKDCLSTYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRVWKPIPGRLKDYIASPKENKY
QSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYKEQIELKADDLSFINRIKKWQQESANKSQYSMNDIHKELLN
TFIYVYTPEGEVVELPFGSNSIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKSYTKTKIKRIAIDPECSPTTGEDIIGIIHKDEIIVHNEN
CQKLKSYKKPQLIEVEWEATPTRKVHHIILLLKELKGIFSYLENIFTLNDVRLISEKIEDCGNGHGITNIIVSSNAKNIT
KIISALKENPNILQIMQIEEDIKNYDN

Sequences:

>Translated_667_residues
MIQAYEIAHLIKINDLEKARNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGEPYIIHPIMVSLFLAKFQLDF
KATIAGLLHDVLEDTNVEKEEIVKEFDEEILSLIDGVTKIHDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHN
MTTLSYLPKNRQDRIAKDCLSTYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRVWKPIPGRLKDYIASPKENKY
QSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYKEQIELKADDLSFINRIKKWQQESANKSQYSMNDIHKELLN
TFIYVYTPEGEVVELPFGSNSIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKSYTKTKIKRIAIDPECSPTTGEDIIGIIHKDEIIVHNEN
CQKLKSYKKPQLIEVEWEATPTRKVHHIILLLKELKGIFSYLENIFTLNDVRLISEKIEDCGNGHGITNIIVSSNAKNIT
KIISALKENPNILQIMQIEEDIKNYDN
>Mature_667_residues
MIQAYEIAHLIKINDLEKARNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGEPYIIHPIMVSLFLAKFQLDF
KATIAGLLHDVLEDTNVEKEEIVKEFDEEILSLIDGVTKIHDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHN
MTTLSYLPKNRQDRIAKDCLSTYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRVWKPIPGRLKDYIASPKENKY
QSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYKEQIELKADDLSFINRIKKWQQESANKSQYSMNDIHKELLN
TFIYVYTPEGEVVELPFGSNSIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKSYTKTKIKRIAIDPECSPTTGEDIIGIIHKDEIIVHNEN
CQKLKSYKKPQLIEVEWEATPTRKVHHIILLLKELKGIFSYLENIFTLNDVRLISEKIEDCGNGHGITNIIVSSNAKNIT
KIISALKENPNILQIMQIEEDIKNYDN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain

Homologues:

Organism=Escherichia coli, GI1790082, Length=611, Percent_Identity=35.3518821603928, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1789147, Length=450, Percent_Identity=35.3333333333333, Blast_Score=301, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPOT_BORBU (O51216)

Other databases:

- EMBL:   AE000783
- PIR:   F70124
- RefSeq:   NP_212332.1
- ProteinModelPortal:   O51216
- SMR:   O51216
- EnsemblBacteria:   EBBORT00000008909
- GeneID:   1195033
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0198
- TIGR:   BB_0198
- GeneTree:   EBGT00050000007475
- HOGENOM:   HBG594146
- OMA:   EPETFRK
- ProtClustDB:   CLSK507424
- BioCyc:   BBUR224326:BB_0198-MONOMER
- BRENDA:   3.1.7.2
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676
- Gene3D:   G3DSA:3.10.20.30
- SMART:   SM00471
- SMART:   SM00954
- TIGRFAMs:   TIGR00691

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS; SSF81271 TGS-like

EC number: =3.1.7.2

Molecular weight: Translated: 77764; Mature: 77764

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQAYEIAHLIKINDLEKARNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGE
CCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
PYIIHPIMVSLFLAKFQLDFKATIAGLLHDVLEDTNVEKEEIVKEFDEEILSLIDGVTKI
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
HDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHNMTTLSYLPKNRQDRIAKDCL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCHHHHHHCCCCHHHHHHHHHH
STYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRV
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHH
WKPIPGRLKDYIASPKENKYQSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYK
HCCCCHHHHHHHCCCCCCHHHHHEEEEECCCCCCEEEEEEEHHHHHHHHHCCHHEEEEEH
EQIELKADDLSFINRIKKWQQESANKSQYSMNDIHKELLNTFIYVYTPEGEVVELPFGSN
HHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEECCCCCEEEECCCCC
SIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
CCEEEEEEEECCCCCEEEEEECCCHHHHHCCCCCCCEEEEEECCCCCCCEEEECCHHHHH
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKSYTKTKIKRIAIDPECSPT
HHHHHHHHHCCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
TGEDIIGIIHKDEIIVHNENCQKLKSYKKPQLIEVEWEATPTRKVHHIILLLKELKGIFS
CCCHHEEEEECCEEEEECCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
YLENIFTLNDVRLISEKIEDCGNGHGITNIIVSSNAKNITKIISALKENPNILQIMQIEE
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEHHH
DIKNYDN
HHCCCCC
>Mature Secondary Structure
MIQAYEIAHLIKINDLEKARNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGE
CCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
PYIIHPIMVSLFLAKFQLDFKATIAGLLHDVLEDTNVEKEEIVKEFDEEILSLIDGVTKI
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
HDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHNMTTLSYLPKNRQDRIAKDCL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCHHHHHHCCCCHHHHHHHHHH
STYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRV
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHH
WKPIPGRLKDYIASPKENKYQSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYK
HCCCCHHHHHHHCCCCCCHHHHHEEEEECCCCCCEEEEEEEHHHHHHHHHCCHHEEEEEH
EQIELKADDLSFINRIKKWQQESANKSQYSMNDIHKELLNTFIYVYTPEGEVVELPFGSN
HHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEECCCCCEEEECCCCC
SIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
CCEEEEEEEECCCCCEEEEEECCCHHHHHCCCCCCCEEEEEECCCCCCCEEEECCHHHHH
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKSYTKTKIKRIAIDPECSPT
HHHHHHHHHCCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
TGEDIIGIIHKDEIIVHNENCQKLKSYKKPQLIEVEWEATPTRKVHHIILLLKELKGIFS
CCCHHEEEEECCEEEEECCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
YLENIFTLNDVRLISEKIEDCGNGHGITNIIVSSNAKNITKIISALKENPNILQIMQIEE
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEHHH
DIKNYDN
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685