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The map label for this gene is prfA [H]

Identifier: 15594541

GI number: 15594541

Start: 193800

End: 194873

Strand: Direct

Name: prfA [H]

Synonym: BB0196

Alternate gene names: 15594541

Gene position: 193800-194873 (Clockwise)

Preceding gene: 15594538

Following gene: 15594542

Centisome position: 21.28

GC content: 29.61

Gene sequence:

>1074_bases
ATGCTTTTAGAAAAATTAAATTCAGCAACAAGCAAGATTAAGTTGATAGAAGAAAAATTACAAGATATAAATCTAATTAA
AGATCAAAAAAAATATTCAAAAATAATAAAAGAATATACATATTTAGAAAAAATAAATACTAAAAAAATTGAATACGAAA
AAATATTAAGCCAAATCAATGATACCAAAACCATCTTGGAAAAAGAAGATCAACAAGAAATGAAAGAGCTAATAAAACAA
GAACTAATTGATCTGGACAAAAAAAAAGAAGATCTTGAACATCAAATAAAAATACTACTTTTGCCTCAAGATGAAAATGA
TAGCAAAAATATAATAATTGAAATTAGGGCTGGAACAGGTGGAGAAGAAGCTGCTCTTTTTGCAAACAATCTTTATAGTA
TGTATATAAAATATTCAGAGAAAAAAAAATGGAAAACAGAAATTATAAACTTCAATGAAACAGAACTTGGGGGATTTAAA
GAAATAATATTTGAAATTAAAGGAAAAGATGTATTTAAAAAATTAAAATACGAAAGCGGTGTTCACAGGGTACAAAGAAT
CCCTATAACAGAGTCTAACGGAAGGCTTCAAACTTCAGCTGCTACTGTAGCAGTGCTACCTAATATTGAAGAAACTGAAA
TTGACATCAATGAAAAAGATTTAAGAATTGATGTTTACAGATCATCTGGAGCTGGTGGGCAACACGTCAATACAACTGAT
TCTGCAGTCAGAATAACGCACCTACCAACAGGAATTGTTGTACAGTGCCAAAACGAAAGAAGCCAGCATAAAAACAAAGA
TCAAGCAATGAAAATATTAAGAGCAAGGCTTTATGAATTTGAAGATTCAAAAAAACAAGAGCAAAGATCAAGCAATAGAA
AACAACAGGTCGGCTCAGGAGATAGATCTGAAAGAATTAGAACCTATAATTTTCCTCAAAATAGAATAACAGATCATAGA
GCAAACATCACTCTTTATAAATTAGAAGAATTTATGCAAGGAGAACTTGACCCACTTCTTGATCCTTTGATGATAGCACT
TCAAGAACAAGAATTAAAAAGCAATAGCATATAG

Upstream 100 bases:

>100_bases
TAAAACATAAATATGGGAATCTACACAAAACTTATTAAAAAGTCTAACAAAAAAATCTAAAATTAATATAAACTATTACT
ATCTTTCAGGAGAAACCATA

Downstream 100 bases:

>100_bases
CAATGAATATAAACGAAGTTATAAATTATGCTAAAGGTAMAAATTTAGATACAATAGAAGCACTACTAATACTTGAATTA
ATTTTAAAAACCAAAAAAGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLLEKLNSATSKIKLIEEKLQDINLIKDQKKYSKIIKEYTYLEKINTKKIEYEKILSQINDTKTILEKEDQQEMKELIKQ
ELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTGGEEAALFANNLYSMYIKYSEKKKWKTEIINFNETELGGFK
EIIFEIKGKDVFKKLKYESGVHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSGDRSERIRTYNFPQNRITDHR
ANITLYKLEEFMQGELDPLLDPLMIALQEQELKSNSI

Sequences:

>Translated_357_residues
MLLEKLNSATSKIKLIEEKLQDINLIKDQKKYSKIIKEYTYLEKINTKKIEYEKILSQINDTKTILEKEDQQEMKELIKQ
ELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTGGEEAALFANNLYSMYIKYSEKKKWKTEIINFNETELGGFK
EIIFEIKGKDVFKKLKYESGVHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSGDRSERIRTYNFPQNRITDHR
ANITLYKLEEFMQGELDPLLDPLMIALQEQELKSNSI
>Mature_357_residues
MLLEKLNSATSKIKLIEEKLQDINLIKDQKKYSKIIKEYTYLEKINTKKIEYEKILSQINDTKTILEKEDQQEMKELIKQ
ELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTGGEEAALFANNLYSMYIKYSEKKKWKTEIINFNETELGGFK
EIIFEIKGKDVFKKLKYESGVHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSGDRSERIRTYNFPQNRITDHR
ANITLYKLEEFMQGELDPLLDPLMIALQEQELKSNSI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=50, Blast_Score=298, Evalue=4e-81,
Organism=Homo sapiens, GI34577120, Length=309, Percent_Identity=41.4239482200647, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=46.1139896373057, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=49.3036211699164, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI2367172, Length=291, Percent_Identity=41.2371134020619, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=320, Percent_Identity=36.25, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=333, Percent_Identity=45.3453453453453, Blast_Score=245, Evalue=8e-66,
Organism=Drosophila melanogaster, GI19921226, Length=337, Percent_Identity=40.3560830860534, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41533; Mature: 41533

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEKLNSATSKIKLIEEKLQDINLIKDQKKYSKIIKEYTYLEKINTKKIEYEKILSQIN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
DTKTILEKEDQQEMKELIKQELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEEEEEEECCCC
GEEAALFANNLYSMYIKYSEKKKWKTEIINFNETELGGFKEIIFEIKGKDVFKKLKYESG
CCHHHHHHHHHEEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
VHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEECCCCCCEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSG
CEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQNRITDHRANITLYKLEEFMQGELDPLLDPLMIALQEQELKSNSI
CCCCCCEEECCCHHHCCCCCCCEEHEEHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLLEKLNSATSKIKLIEEKLQDINLIKDQKKYSKIIKEYTYLEKINTKKIEYEKILSQIN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
DTKTILEKEDQQEMKELIKQELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEEEEEEECCCC
GEEAALFANNLYSMYIKYSEKKKWKTEIINFNETELGGFKEIIFEIKGKDVFKKLKYESG
CCHHHHHHHHHEEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
VHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEECCCCCCEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSG
CEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQNRITDHRANITLYKLEEFMQGELDPLLDPLMIALQEQELKSNSI
CCCCCCEEECCCHHHCCCCCCCEEHEEHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA