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The map label for this gene is hisS

Identifier: 15594480

GI number: 15594480

Start: 132472

End: 133845

Strand: Reverse

Name: hisS

Synonym: BB0135

Alternate gene names: 15594480

Gene position: 133845-132472 (Counterclockwise)

Preceding gene: 15594482

Following gene: 15594478

Centisome position: 14.7

GC content: 28.17

Gene sequence:

>1374_bases
GTGATGGATATTAAAACTTTAAAAGGCTTTAAAGATTATTTACCGAAGGATTCTTTGATTCGAATTCATATTGTTAGGCA
AATATTTAGTGTTCTTAATTCTTATAATTTTGACCTAATAGATACTCCAGTTCTTGAATATTCAGATTTGCTTTTAAAAA
AGAGTGGGGATGAGACTGAAAAGCAAATTTATAGGTTTAAGGATAATGGAGGCAGAGATGTTTCCATGCGATTTGATTTA
ACTGTTCCTTTTGCCAGATTTGTTGCTACAAATATTTCTGCTTTAAAGCTTCCTTTTAGACGTTCTCAATTTGGAAAAGT
TTTCAGAGGAGAAAATTCTCAAAAGGGTAGATACAGAGAATTTATGCAGTTTGATTTTGATATAGTTGGAGAGGACACTT
TTAGGGGCGATGCCGAGATTCTTTCTGTTGTATATTATGGACTTGAAGAGATTTTTTTGAATTTTATAGAAGGCATTAAT
AAAAAATTTATTATCCATTATTCTCATATTGGTATATTGAATTCTTTTTTTGAAAAATTAGGACTTAAAGAAAAATCTAT
TTTTATTTTAAGAAATATTGACAAAATAGATAAAATAGGAATTGATAAGGTTAAAGAAGCTTTGCTTCTTGAGATTGAAA
AAGAAGCGGTAGATTCAATCTTAAGTTTAGTGAGTTTGCAAGGTACTTTTAAAGACAAAATACAAGCTTTAAAAAGTATT
TTAGGCGATAATGAGTCTATCAAGAGAGTAGAAGATGTTTTTCAGCATCTTAGTTTATTAAAAATTCAAGATTCTTTTAA
CTTGAATCTTAAAATATCGCGTGGACTTGATTATTATACGGGGATTGTTTTTGAATCTGAGGTGTTTGGCAGTAATATGG
GTAGTGTTTGCAGTGGGGGAAGATATGATAATTTGGTTTCTTCGTTCTCAAACTCTATTCAAAAAATTTCAGGGGTTGGA
GGATCTTTTGGTGTTGACAGAATAAAGGATATAATTGATCTTGAAAAGTTTAGTTATATTAAAATATTTGTCACTAAGGC
TAGATCTAAAGTTTTAATTGTAAATTTAGATAGTGCTTTGCAAAATTATTATTATGAACTTGCTACTAGATTTAGAAATC
ATGATTATTCCAAGGTTAAAAATATTTCTTGTGAAGTCTATTTTAAAAATAAAAATGGTAAAAATATTAAAGAACAAATA
GAATATGCTTTAAGCAAAGAAATAAGATTTTTAGTTTTTGTTGGTCAAGAAGAATACAAAGAAAATAAAATGAAAGTAAG
AGATTTGACAAAAAAAGAAGAGTTGCTGCTTTCTTTTGAAGAATCAATAAATCTTATCAAATGTAATGAAAAGTTGTTGT
GCACCCCTTTTTAA

Upstream 100 bases:

>100_bases
ACTATATGAAATAAAAAATTATTCATTTTTTTTTGCATCTATATTATTATATGTTATTATATTCTGAAAAAGTAAATTAC
TATATAGCAGAGGGTATAAT

Downstream 100 bases:

>100_bases
TCTTAAATGTTAATTATTTTTTGAAATTTTGGCAAAGATATTAGGATTAATTTTCATTTTTATCTATTTTTCTAATTGTT
ATTGCATTTAAGGTAAGTTT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MMDIKTLKGFKDYLPKDSLIRIHIVRQIFSVLNSYNFDLIDTPVLEYSDLLLKKSGDETEKQIYRFKDNGGRDVSMRFDL
TVPFARFVATNISALKLPFRRSQFGKVFRGENSQKGRYREFMQFDFDIVGEDTFRGDAEILSVVYYGLEEIFLNFIEGIN
KKFIIHYSHIGILNSFFEKLGLKEKSIFILRNIDKIDKIGIDKVKEALLLEIEKEAVDSILSLVSLQGTFKDKIQALKSI
LGDNESIKRVEDVFQHLSLLKIQDSFNLNLKISRGLDYYTGIVFESEVFGSNMGSVCSGGRYDNLVSSFSNSIQKISGVG
GSFGVDRIKDIIDLEKFSYIKIFVTKARSKVLIVNLDSALQNYYYELATRFRNHDYSKVKNISCEVYFKNKNGKNIKEQI
EYALSKEIRFLVFVGQEEYKENKMKVRDLTKKEELLLSFEESINLIKCNEKLLCTPF

Sequences:

>Translated_457_residues
MMDIKTLKGFKDYLPKDSLIRIHIVRQIFSVLNSYNFDLIDTPVLEYSDLLLKKSGDETEKQIYRFKDNGGRDVSMRFDL
TVPFARFVATNISALKLPFRRSQFGKVFRGENSQKGRYREFMQFDFDIVGEDTFRGDAEILSVVYYGLEEIFLNFIEGIN
KKFIIHYSHIGILNSFFEKLGLKEKSIFILRNIDKIDKIGIDKVKEALLLEIEKEAVDSILSLVSLQGTFKDKIQALKSI
LGDNESIKRVEDVFQHLSLLKIQDSFNLNLKISRGLDYYTGIVFESEVFGSNMGSVCSGGRYDNLVSSFSNSIQKISGVG
GSFGVDRIKDIIDLEKFSYIKIFVTKARSKVLIVNLDSALQNYYYELATRFRNHDYSKVKNISCEVYFKNKNGKNIKEQI
EYALSKEIRFLVFVGQEEYKENKMKVRDLTKKEELLLSFEESINLIKCNEKLLCTPF
>Mature_457_residues
MMDIKTLKGFKDYLPKDSLIRIHIVRQIFSVLNSYNFDLIDTPVLEYSDLLLKKSGDETEKQIYRFKDNGGRDVSMRFDL
TVPFARFVATNISALKLPFRRSQFGKVFRGENSQKGRYREFMQFDFDIVGEDTFRGDAEILSVVYYGLEEIFLNFIEGIN
KKFIIHYSHIGILNSFFEKLGLKEKSIFILRNIDKIDKIGIDKVKEALLLEIEKEAVDSILSLVSLQGTFKDKIQALKSI
LGDNESIKRVEDVFQHLSLLKIQDSFNLNLKISRGLDYYTGIVFESEVFGSNMGSVCSGGRYDNLVSSFSNSIQKISGVG
GSFGVDRIKDIIDLEKFSYIKIFVTKARSKVLIVNLDSALQNYYYELATRFRNHDYSKVKNISCEVYFKNKNGKNIKEQI
EYALSKEIRFLVFVGQEEYKENKMKVRDLTKKEELLLSFEESINLIKCNEKLLCTPF

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=465, Percent_Identity=31.3978494623656, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI15029520, Length=466, Percent_Identity=31.5450643776824, Blast_Score=191, Evalue=9e-49,
Organism=Escherichia coli, GI1788861, Length=444, Percent_Identity=25, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71993693, Length=482, Percent_Identity=29.045643153527, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71993686, Length=482, Percent_Identity=29.045643153527, Blast_Score=175, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6325290, Length=474, Percent_Identity=32.4894514767932, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6320489, Length=281, Percent_Identity=25.9786476868327, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24643061, Length=479, Percent_Identity=29.6450939457203, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24643059, Length=497, Percent_Identity=28.7726358148893, Blast_Score=175, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_BORBU (O51160)

Other databases:

- EMBL:   AE000783
- PIR:   G70116
- RefSeq:   NP_212269.1
- ProteinModelPortal:   O51160
- SMR:   O51160
- EnsemblBacteria:   EBBORT00000008874
- GeneID:   1194970
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0135
- NMPDR:   fig|224326.1.peg.519
- TIGR:   BB_0135
- GeneTree:   EBGT00050000006869
- HOGENOM:   HBG616575
- OMA:   IGKVFRD
- PhylomeDB:   O51160
- ProtClustDB:   PRK00037
- BioCyc:   BBUR224326:BB_0135-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 52850; Mature: 52850

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDIKTLKGFKDYLPKDSLIRIHIVRQIFSVLNSYNFDLIDTPVLEYSDLLLKKSGDETE
CCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHCCCCHHH
KQIYRFKDNGGRDVSMRFDLTVPFARFVATNISALKLPFRRSQFGKVFRGENSQKGRYRE
HHHHHHCCCCCCEEEEEEEEECHHHHHHHCCHHHEECCCHHHHHHHHHCCCCCCCCHHHH
FMQFDFDIVGEDTFRGDAEILSVVYYGLEEIFLNFIEGINKKFIIHYSHIGILNSFFEKL
HHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHH
GLKEKSIFILRNIDKIDKIGIDKVKEALLLEIEKEAVDSILSLVSLQGTFKDKIQALKSI
CCCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LGDNESIKRVEDVFQHLSLLKIQDSFNLNLKISRGLDYYTGIVFESEVFGSNMGSVCSGG
HCCCHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCHHHCEEEEHHHHCCCCCCCCCCC
RYDNLVSSFSNSIQKISGVGGSFGVDRIKDIIDLEKFSYIKIFVTKARSKVLIVNLDSAL
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCEEEEEEEECCCEEEEEECHHHH
QNYYYELATRFRNHDYSKVKNISCEVYFKNKNGKNIKEQIEYALSKEIRFLVFVGQEEYK
HHHHHHHHHHHCCCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHH
ENKMKVRDLTKKEELLLSFEESINLIKCNEKLLCTPF
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCC
>Mature Secondary Structure
MMDIKTLKGFKDYLPKDSLIRIHIVRQIFSVLNSYNFDLIDTPVLEYSDLLLKKSGDETE
CCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHCCCCHHH
KQIYRFKDNGGRDVSMRFDLTVPFARFVATNISALKLPFRRSQFGKVFRGENSQKGRYRE
HHHHHHCCCCCCEEEEEEEEECHHHHHHHCCHHHEECCCHHHHHHHHHCCCCCCCCHHHH
FMQFDFDIVGEDTFRGDAEILSVVYYGLEEIFLNFIEGINKKFIIHYSHIGILNSFFEKL
HHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHH
GLKEKSIFILRNIDKIDKIGIDKVKEALLLEIEKEAVDSILSLVSLQGTFKDKIQALKSI
CCCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LGDNESIKRVEDVFQHLSLLKIQDSFNLNLKISRGLDYYTGIVFESEVFGSNMGSVCSGG
HCCCHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCHHHCEEEEHHHHCCCCCCCCCCC
RYDNLVSSFSNSIQKISGVGGSFGVDRIKDIIDLEKFSYIKIFVTKARSKVLIVNLDSAL
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCEEEEEEEECCCEEEEEECHHHH
QNYYYELATRFRNHDYSKVKNISCEVYFKNKNGKNIKEQIEYALSKEIRFLVFVGQEEYK
HHHHHHHHHHHCCCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHH
ENKMKVRDLTKKEELLLSFEESINLIKCNEKLLCTPF
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685