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The map label for this gene is yaeL [C]

Identifier: 15594464

GI number: 15594464

Start: 115505

End: 116818

Strand: Reverse

Name: yaeL [C]

Synonym: BB0118

Alternate gene names: 15594464

Gene position: 116818-115505 (Counterclockwise)

Preceding gene: 15595197

Following gene: 15594462

Centisome position: 12.83

GC content: 27.17

Gene sequence:

>1314_bases
TTGGAGGAAATTATGTATATTCTTTTTAGTGTGTTGGCTCTCAGTTTTATAATATTTATTCATGAGCTAGGGCACTTTTT
ATTTGCTAAACTTTTTAAAGTTAAGGTTGAAGTTTTTTCTGTGGGTATAGGTCCTAGCATATTAAAGTTTAAAATTAATA
ATACTGAGTATAGACTTTCCCCAATCCTTCTAGGAGGATATTGTAAGCTTAAGGGATTTGATCACTTAGAAAAAGAGCTT
AAGGCAAATAAAGAATTAGAAGCAGATAAAGATTCTTTGTTTGGAATTTCACATTTTAAAAAAATCTTAATATATTTTGC
AGGTCCGTTGTTTAATTTGATTTTTTCATTTATTGTTTTTATTTTTATAAGTATGGCGGGTGTTATATATTTTGATTATT
CTTCAAGAGTTAGTATTTTAAATAAAGATTCTTTATTAAAAGATAAATTTAGAGACGGTGATGTTATATTAAAGGTTAAT
GATAAAAAAATTAAATATTTTTCTGATTTAAGAAAATTCATTCCTGAGGAAAAATCTACAGTTATGTTTGATGTTTTAAG
GGAAAAAGAAAATATTACTTTTAAAGAGACTGTTAGTTTGCAAGATTTTTTAAAAGAAATTGGCCCTTGGGCTGATCTTG
TAATAGCAGATGTGGTTTCAAATTCGCCTGCTAAGATTGCGGGAATGAAACCGGGTGACGAAATAATTAGCATTGATAAT
GTTATTTTGAAAAATAAAAGAGATTTAGATTATTTTCTTAAGAATTTAAATTCGGATGTTGTGGAAATTAAGTTTTCTAG
AAATGGAGAGATTTTTTCTTCTAAATTAGTATTTCATGATAAAAATAAAATGATTGGCATATATTTTTCACCACCTTTAA
AAAGGGTAGTTAAAGTAGAAAATGTTTCAAGTGCCATTAAGAATTCTTTTTTTAAGGTAGTAAGTGCTTTGCAAGACATT
TTATATTCTATTTTTTTATTAATGACAAATTTTTTAAATGCTTCTAAGAGTGTATCAGGCCCTGTTGGAATTGTAGGCAT
TCTCTCTTCATCTTATTCTTTAGGGATATTGTATTGGATTAATAGCATTTCTTTCTTGAGCTTAATTCTTGCTGGTATGA
ATCTATTTTTTATTGTAATACCTATTTTTGATGGGGGACAAATTTTTATCAGCTTTATTGAGCTTTTGCGTGGAAAGAGA
TTTAAGGCCAAAACCATTTATTCTTTTTATAGCTTTGGTATTTTTTTTGGGCTGTTTCTTTTTGGTTTAGGCCTTTTTAA
TGATTTGAAAGGTCTTTTAAATATATTTAATTAA

Upstream 100 bases:

>100_bases
TTCTGGGAAAATCGTTCCTGGTAGAGGCGGAGCTCTTGATTCGATTGACTCTTTTCTTTTAACAGGCCCGATATTTTATT
TATACCTATCTTAGTGATTT

Downstream 100 bases:

>100_bases
AAATTAGAAATGTATTTTAGCATAAGCCAAAAGTGAGATGCTGATGCGATTATTATTAATATATGAAATACATCATGCAT
TTTCATGTTAATTGTTGGAT

Product: zinc protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MEEIMYILFSVLALSFIIFIHELGHFLFAKLFKVKVEVFSVGIGPSILKFKINNTEYRLSPILLGGYCKLKGFDHLEKEL
KANKELEADKDSLFGISHFKKILIYFAGPLFNLIFSFIVFIFISMAGVIYFDYSSRVSILNKDSLLKDKFRDGDVILKVN
DKKIKYFSDLRKFIPEEKSTVMFDVLREKENITFKETVSLQDFLKEIGPWADLVIADVVSNSPAKIAGMKPGDEIISIDN
VILKNKRDLDYFLKNLNSDVVEIKFSRNGEIFSSKLVFHDKNKMIGIYFSPPLKRVVKVENVSSAIKNSFFKVVSALQDI
LYSIFLLMTNFLNASKSVSGPVGIVGILSSSYSLGILYWINSISFLSLILAGMNLFFIVIPIFDGGQIFISFIELLRGKR
FKAKTIYSFYSFGIFFGLFLFGLGLFNDLKGLLNIFN

Sequences:

>Translated_437_residues
MEEIMYILFSVLALSFIIFIHELGHFLFAKLFKVKVEVFSVGIGPSILKFKINNTEYRLSPILLGGYCKLKGFDHLEKEL
KANKELEADKDSLFGISHFKKILIYFAGPLFNLIFSFIVFIFISMAGVIYFDYSSRVSILNKDSLLKDKFRDGDVILKVN
DKKIKYFSDLRKFIPEEKSTVMFDVLREKENITFKETVSLQDFLKEIGPWADLVIADVVSNSPAKIAGMKPGDEIISIDN
VILKNKRDLDYFLKNLNSDVVEIKFSRNGEIFSSKLVFHDKNKMIGIYFSPPLKRVVKVENVSSAIKNSFFKVVSALQDI
LYSIFLLMTNFLNASKSVSGPVGIVGILSSSYSLGILYWINSISFLSLILAGMNLFFIVIPIFDGGQIFISFIELLRGKR
FKAKTIYSFYSFGIFFGLFLFGLGLFNDLKGLLNIFN
>Mature_437_residues
MEEIMYILFSVLALSFIIFIHELGHFLFAKLFKVKVEVFSVGIGPSILKFKINNTEYRLSPILLGGYCKLKGFDHLEKEL
KANKELEADKDSLFGISHFKKILIYFAGPLFNLIFSFIVFIFISMAGVIYFDYSSRVSILNKDSLLKDKFRDGDVILKVN
DKKIKYFSDLRKFIPEEKSTVMFDVLREKENITFKETVSLQDFLKEIGPWADLVIADVVSNSPAKIAGMKPGDEIISIDN
VILKNKRDLDYFLKNLNSDVVEIKFSRNGEIFSSKLVFHDKNKMIGIYFSPPLKRVVKVENVSSAIKNSFFKVVSALQDI
LYSIFLLMTNFLNASKSVSGPVGIVGILSSSYSLGILYWINSISFLSLILAGMNLFFIVIPIFDGGQIFISFIELLRGKR
FKAKTIYSFYSFGIFFGLFLFGLGLFNDLKGLLNIFN

Specific function: Unknown

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain

Homologues:

Organism=Escherichia coli, GI1786373, Length=449, Percent_Identity=24.7216035634744, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y118_BORBU (O51145)

Other databases:

- EMBL:   AE000783
- PIR:   F70114
- RefSeq:   NP_212252.1
- ProteinModelPortal:   O51145
- EnsemblBacteria:   EBBORT00000008378
- GeneID:   1194954
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0118
- NMPDR:   fig|224326.1.peg.502
- TIGR:   BB_0118
- GeneTree:   EBGT00050000007368
- HOGENOM:   HBG664087
- OMA:   GMIDESM
- ProtClustDB:   CLSK507367
- BioCyc:   BBUR224326:BB_0118-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915
- SMART:   SM00228
- TIGRFAMs:   TIGR00054

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50; SSF50156 PDZ

EC number: NA

Molecular weight: Translated: 49741; Mature: 49741

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50106 PDZ; PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 22-22

Signals:

None

Transmembrane regions:

HASH(0x969ff24)-; HASH(0xbdc7c14)-; HASH(0xb3b5930)-; HASH(0xb2c5df4)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIMYILFSVLALSFIIFIHELGHFLFAKLFKVKVEVFSVGIGPSILKFKINNTEYRLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCEEEEE
PILLGGYCKLKGFDHLEKELKANKELEADKDSLFGISHFKKILIYFAGPLFNLIFSFIVF
EEEEECEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFISMAGVIYFDYSSRVSILNKDSLLKDKFRDGDVILKVNDKKIKYFSDLRKFIPEEKST
HHHHHHHEEEEECCCCEEEECCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCH
VMFDVLREKENITFKETVSLQDFLKEIGPWADLVIADVVSNSPAKIAGMKPGDEIISIDN
HHHHHHHHCCCCCHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCEEEHHH
VILKNKRDLDYFLKNLNSDVVEIKFSRNGEIFSSKLVFHDKNKMIGIYFSPPLKRVVKVE
HHHCCCCCHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEECCCEEEEEECCHHHHHHHHH
NVSSAIKNSFFKVVSALQDILYSIFLLMTNFLNASKSVSGPVGIVGILSSSYSLGILYWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHH
NSISFLSLILAGMNLFFIVIPIFDGGQIFISFIELLRGKRFKAKTIYSFYSFGIFFGLFL
HHHHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FGLGLFNDLKGLLNIFN
HHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MEEIMYILFSVLALSFIIFIHELGHFLFAKLFKVKVEVFSVGIGPSILKFKINNTEYRLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCEEEEE
PILLGGYCKLKGFDHLEKELKANKELEADKDSLFGISHFKKILIYFAGPLFNLIFSFIVF
EEEEECEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFISMAGVIYFDYSSRVSILNKDSLLKDKFRDGDVILKVNDKKIKYFSDLRKFIPEEKST
HHHHHHHEEEEECCCCEEEECCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCH
VMFDVLREKENITFKETVSLQDFLKEIGPWADLVIADVVSNSPAKIAGMKPGDEIISIDN
HHHHHHHHCCCCCHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCEEEHHH
VILKNKRDLDYFLKNLNSDVVEIKFSRNGEIFSSKLVFHDKNKMIGIYFSPPLKRVVKVE
HHHCCCCCHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEECCCEEEEEECCHHHHHHHHH
NVSSAIKNSFFKVVSALQDILYSIFLLMTNFLNASKSVSGPVGIVGILSSSYSLGILYWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHH
NSISFLSLILAGMNLFFIVIPIFDGGQIFISFIELLRGKRFKAKTIYSFYSFGIFFGLFL
HHHHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FGLGLFNDLKGLLNIFN
HHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9403685