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The map label for this gene is prfB [H]

Identifier: 15594420

GI number: 15594420

Start: 70173

End: 71219

Strand: Direct

Name: prfB [H]

Synonym: BB0074

Alternate gene names: 15594420

Gene position: 70173-71219 (Clockwise)

Preceding gene: 15594419

Following gene: 15594421

Centisome position: 7.71

GC content: 32.95

Gene sequence:

>1047_bases
ATGCCGAAGACATTTGGAGGAAGCTTTGACAAAAATGAAATTCAAGCTAAAATCGAAAAATATGAAAAAGAAATAAACCA
GAAAAATTTTTGGAATGATCCTAAAAGAGCTCAAAAAGTAATTAAGGCTCAAAGTATTTTAAAAAATAAAATTGATCCGT
GGGAAGAGCTAATAAATAAAATAAAAGACTTAAGCGATTTGTGCGAAATTGCCGAAAATGAAAAAGATACTAACGGCTTG
GAAATTGAATTTAATACGCTTGAAAAACAATACAAAGATTTACTCACAATTTCTTACTTTAAAGAAGAGCTTGACGCAAA
CAATGCTTTTTTAACTATTCATTCTGGGGCTGGGGGCACTGAAGCATGTGACTGGGTCGCAATGCTTTACAGAATGTATT
CAAGATACGCTGAGAGAAAAAAATACAAAACAGAACTTATTGATTTACTCGAAGCAGAAGGTGGAATAAAATCTGTTACA
ATAGAAATCAAAGGTGAGTATGCTTATGGGCTATTGAAAAGTGAAGTTGGAATACATCGTCTTATAAGAATTTCTCCTTT
TGATGCTGCTAAAAAAAGGCATACCTCTTTTGCATCAGTATTTGTTGACCCTGTTATTGATGACAAAATAGAAATAACAA
TCAAACCAGAAGACATAAGAATTGATACATACAGAGCATCGGGAGCCGGAGGACAACACGTCAATAAAACATCCTCTGCT
GTAAGAATAACTCACATTGAAACAGGAATAGTAACTCAGTCTCAAAGCGACAGAAGCCAGCACAAAAACAAAGACTTGGC
AATGAAAGTTTTAAAATCAAGACTTTATGAATACTATAAGAGCAAAGAAGATGAAAAAAACAAATCCAAGCAAGACACAA
AAAAAGAAATTTCTTGGGGCAACCAAATAAGATCTTACGTATTTCAACCTTACAATTTGGTAAAAGATCACAGAACAAAA
TTTGAAAATTCAAACACCACTTCGGTTATGGACGGAAACATAGACAATTTCATAGAAGAGTATCTAAAATGGAAAAGCTT
AAACTAA

Upstream 100 bases:

>100_bases
ATATTTATTTTATCTTTAATTCCAAATATAATTATATTAATATACAATCAATAATAGCGAGAAATAAAAATGAAAGAAAA
AATAAATACATTGCTCAAAC

Downstream 100 bases:

>100_bases
AGCTAGCAATACCATTGCTAGTATTTACAATATGCAAAATACATTCTCAAAGTAATATTGAATACAATTTTTCCTATATC
ATTAATACAAAAAAAGAAAA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MPKTFGGSFDKNEIQAKIEKYEKEINQKNFWNDPKRAQKVIKAQSILKNKIDPWEELINKIKDLSDLCEIAENEKDTNGL
EIEFNTLEKQYKDLLTISYFKEELDANNAFLTIHSGAGGTEACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKSVT
IEIKGEYAYGLLKSEVGIHRLIRISPFDAAKKRHTSFASVFVDPVIDDKIEITIKPEDIRIDTYRASGAGGQHVNKTSSA
VRITHIETGIVTQSQSDRSQHKNKDLAMKVLKSRLYEYYKSKEDEKNKSKQDTKKEISWGNQIRSYVFQPYNLVKDHRTK
FENSNTTSVMDGNIDNFIEEYLKWKSLN

Sequences:

>Translated_348_residues
MPKTFGGSFDKNEIQAKIEKYEKEINQKNFWNDPKRAQKVIKAQSILKNKIDPWEELINKIKDLSDLCEIAENEKDTNGL
EIEFNTLEKQYKDLLTISYFKEELDANNAFLTIHSGAGGTEACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKSVT
IEIKGEYAYGLLKSEVGIHRLIRISPFDAAKKRHTSFASVFVDPVIDDKIEITIKPEDIRIDTYRASGAGGQHVNKTSSA
VRITHIETGIVTQSQSDRSQHKNKDLAMKVLKSRLYEYYKSKEDEKNKSKQDTKKEISWGNQIRSYVFQPYNLVKDHRTK
FENSNTTSVMDGNIDNFIEEYLKWKSLN
>Mature_347_residues
PKTFGGSFDKNEIQAKIEKYEKEINQKNFWNDPKRAQKVIKAQSILKNKIDPWEELINKIKDLSDLCEIAENEKDTNGLE
IEFNTLEKQYKDLLTISYFKEELDANNAFLTIHSGAGGTEACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKSVTI
EIKGEYAYGLLKSEVGIHRLIRISPFDAAKKRHTSFASVFVDPVIDDKIEITIKPEDIRIDTYRASGAGGQHVNKTSSAV
RITHIETGIVTQSQSDRSQHKNKDLAMKVLKSRLYEYYKSKEDEKNKSKQDTKKEISWGNQIRSYVFQPYNLVKDHRTKF
ENSNTTSVMDGNIDNFIEEYLKWKSLN

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=291, Percent_Identity=38.1443298969072, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI34577120, Length=298, Percent_Identity=35.2348993288591, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI166795305, Length=184, Percent_Identity=39.6739130434783, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=48.9736070381232, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1787462, Length=244, Percent_Identity=40.5737704918033, Blast_Score=184, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=281, Percent_Identity=30.6049822064057, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=326, Percent_Identity=34.9693251533742, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19921226, Length=250, Percent_Identity=38, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40269; Mature: 40138

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTFGGSFDKNEIQAKIEKYEKEINQKNFWNDPKRAQKVIKAQSILKNKIDPWEELINK
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
IKDLSDLCEIAENEKDTNGLEIEFNTLEKQYKDLLTISYFKEELDANNAFLTIHSGAGGT
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
EACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKSVTIEIKGEYAYGLLKSEVGIHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHH
LIRISPFDAAKKRHTSFASVFVDPVIDDKIEITIKPEDIRIDTYRASGAGGQHVNKTSSA
HEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCE
VRITHIETGIVTQSQSDRSQHKNKDLAMKVLKSRLYEYYKSKEDEKNKSKQDTKKEISWG
EEEEEEECCCEECCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NQIRSYVFQPYNLVKDHRTKFENSNTTSVMDGNIDNFIEEYLKWKSLN
HHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PKTFGGSFDKNEIQAKIEKYEKEINQKNFWNDPKRAQKVIKAQSILKNKIDPWEELINK
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
IKDLSDLCEIAENEKDTNGLEIEFNTLEKQYKDLLTISYFKEELDANNAFLTIHSGAGGT
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
EACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKSVTIEIKGEYAYGLLKSEVGIHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHH
LIRISPFDAAKKRHTSFASVFVDPVIDDKIEITIKPEDIRIDTYRASGAGGQHVNKTSSA
HEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCE
VRITHIETGIVTQSQSDRSQHKNKDLAMKVLKSRLYEYYKSKEDEKNKSKQDTKKEISWG
EEEEEEECCCEECCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NQIRSYVFQPYNLVKDHRTKFENSNTTSVMDGNIDNFIEEYLKWKSLN
HHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]