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The map label for this gene is lepB [C]

Identifier: 15594377

GI number: 15594377

Start: 28492

End: 29472

Strand: Reverse

Name: lepB [C]

Synonym: BB0031

Alternate gene names: 15594377

Gene position: 29472-28492 (Counterclockwise)

Preceding gene: 15594380

Following gene: 15594376

Centisome position: 3.24

GC content: 28.13

Gene sequence:

>981_bases
ATGTATTTAAGAAGATTGGATAAATTTGCATCTTTTTTGGTACACATTGTTGAGAAATATTTGACATATAGAAAAAGAAA
GAAATATTTTTGCAAATTAAGGGCTAAGAAACGAGGATTTTTGCTTAACTTTTTATTTGATTTTGTAGCAGCGGCAATTT
TTGTATTGGTGATAAATCAATATTTTGTTCAAGCTTATAAAATACCATCAGGATCAATGGAAAATACTCTTCAAATAGGG
GATTTTTTATTTGTAGATAAATTTTCTTACGGTCCTGAGCTTTTACCAGGGTTATTTAAAATTAATGGTTTTAAAGCCCC
AGAAGAATCCGATATTATTATTTTTGAAAATCCAGAGTATAAATCAAAGGGTGTTTTTTTTGATATCTTTCAAAGAATAC
TTTACATGTTAACATTATCTTTTATTGATCTTGATAGAGATGAATATGGCAATCCCAATGTTAGATTTCTTGTGAAAAGA
GGTGCGTTTGCAGATGGTAAAATTGTTAGATTTAATAACGGTAAAGCTTATATTAAAAGAGAAGGCGAGGAAAATTTTAT
TTTAGAAGATTCTTATTGGGATTTGGTTGATAAAAATTTTAAAATTAAAAAAATTGTGGCAAATGAGGACTATGGGATTT
ATGGTGATTTTGCTATGTTTGTTGCTCTTAGCCAGTTAAATATAAATTTAAGTAGCACTCCCGATTTTTCATTTTTTGAT
GTTAGAATGATTGATAGGTTTGAGTTTGAAAGATTGGAATATAAGTATTTATCTGCTTTTATGCCTTATGTGGATTATTA
TATGGAAAAAGCTATAATGAGAGATTATGGTATTTATGTTCCTGATGGATATATTTTGCCAATTGGAGATAATAGAGACA
ATTCTCATGATGGTCGGTTTTTTGGAGTAATCAATAAGAATAAAGTACTTGGAAGAACCTTGATAATATATCTTCCATTT
TCTAGAGTAGGATTTATTTAA

Upstream 100 bases:

>100_bases
AACATTTGAATTCTCGTCAACCTCTTTCATCTTTTAAAGTATAGAAAAGTATTTAAATAAGTGTCAATATTTTGTATTAT
TTAATTCTTGAGGTTAGGTT

Downstream 100 bases:

>100_bases
AGTGGCCCCATATTTAACTTTCGAACAAAGGCTTTTAAGGAAAAGACAAAGAAAAGTTTTTTTTAAATATTTTTTAACAT
TTTTAATTTTAAATTTTTTT

Product: signal peptidase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MYLRRLDKFASFLVHIVEKYLTYRKRKKYFCKLRAKKRGFLLNFLFDFVAAAIFVLVINQYFVQAYKIPSGSMENTLQIG
DFLFVDKFSYGPELLPGLFKINGFKAPEESDIIIFENPEYKSKGVFFDIFQRILYMLTLSFIDLDRDEYGNPNVRFLVKR
GAFADGKIVRFNNGKAYIKREGEENFILEDSYWDLVDKNFKIKKIVANEDYGIYGDFAMFVALSQLNINLSSTPDFSFFD
VRMIDRFEFERLEYKYLSAFMPYVDYYMEKAIMRDYGIYVPDGYILPIGDNRDNSHDGRFFGVINKNKVLGRTLIIYLPF
SRVGFI

Sequences:

>Translated_326_residues
MYLRRLDKFASFLVHIVEKYLTYRKRKKYFCKLRAKKRGFLLNFLFDFVAAAIFVLVINQYFVQAYKIPSGSMENTLQIG
DFLFVDKFSYGPELLPGLFKINGFKAPEESDIIIFENPEYKSKGVFFDIFQRILYMLTLSFIDLDRDEYGNPNVRFLVKR
GAFADGKIVRFNNGKAYIKREGEENFILEDSYWDLVDKNFKIKKIVANEDYGIYGDFAMFVALSQLNINLSSTPDFSFFD
VRMIDRFEFERLEYKYLSAFMPYVDYYMEKAIMRDYGIYVPDGYILPIGDNRDNSHDGRFFGVINKNKVLGRTLIIYLPF
SRVGFI
>Mature_326_residues
MYLRRLDKFASFLVHIVEKYLTYRKRKKYFCKLRAKKRGFLLNFLFDFVAAAIFVLVINQYFVQAYKIPSGSMENTLQIG
DFLFVDKFSYGPELLPGLFKINGFKAPEESDIIIFENPEYKSKGVFFDIFQRILYMLTLSFIDLDRDEYGNPNVRFLVKR
GAFADGKIVRFNNGKAYIKREGEENFILEDSYWDLVDKNFKIKKIVANEDYGIYGDFAMFVALSQLNINLSSTPDFSFFD
VRMIDRFEFERLEYKYLSAFMPYVDYYMEKAIMRDYGIYVPDGYILPIGDNRDNSHDGRFFGVINKNKVLGRTLIIYLPF
SRVGFI

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.21.89

Molecular weight: Translated: 38481; Mature: 38481

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLRRLDKFASFLVHIVEKYLTYRKRKKYFCKLRAKKRGFLLNFLFDFVAAAIFVLVINQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YFVQAYKIPSGSMENTLQIGDFLFVDKFSYGPELLPGLFKINGFKAPEESDIIIFENPEY
HHHHEEECCCCCCCCEEECCCEEEEECCCCCHHHCCHHEEECCCCCCCCCCEEEEECCCC
KSKGVFFDIFQRILYMLTLSFIDLDRDEYGNPNVRFLVKRGAFADGKIVRFNNGKAYIKR
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEEE
EGEENFILEDSYWDLVDKNFKIKKIVANEDYGIYGDFAMFVALSQLNINLSSTPDFSFFD
CCCCCEEEECCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHEEEECCCCCCCEEE
VRMIDRFEFERLEYKYLSAFMPYVDYYMEKAIMRDYGIYVPDGYILPIGDNRDNSHDGRF
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEECCCCCCCCCCCEE
FGVINKNKVLGRTLIIYLPFSRVGFI
EEEECCCCCCCEEEEEEECHHHHCCC
>Mature Secondary Structure
MYLRRLDKFASFLVHIVEKYLTYRKRKKYFCKLRAKKRGFLLNFLFDFVAAAIFVLVINQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YFVQAYKIPSGSMENTLQIGDFLFVDKFSYGPELLPGLFKINGFKAPEESDIIIFENPEY
HHHHEEECCCCCCCCEEECCCEEEEECCCCCHHHCCHHEEECCCCCCCCCCEEEEECCCC
KSKGVFFDIFQRILYMLTLSFIDLDRDEYGNPNVRFLVKRGAFADGKIVRFNNGKAYIKR
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEEE
EGEENFILEDSYWDLVDKNFKIKKIVANEDYGIYGDFAMFVALSQLNINLSSTPDFSFFD
CCCCCEEEECCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHEEEECCCCCCCEEE
VRMIDRFEFERLEYKYLSAFMPYVDYYMEKAIMRDYGIYVPDGYILPIGDNRDNSHDGRF
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEECCCCCCCCCCCEE
FGVINKNKVLGRTLIIYLPFSRVGFI
EEEECCCCCCCEEEEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phospholipids; Triton X-100 [C]

Metal ions: NA

Kcat value (1/min): 523.8 [C]

Specific activity: NA

Km value (mM): 0.52 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.33 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.032 {pro-OmpA-nuclease} 0.0165 {pro-OmpA-nuclease} 1 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-CONH2}} [C]

Substrates: NA

Specific reaction: NA

General reaction: Peptide bond hydrolysis [C]

Inhibitor: 1-Ethyl -3-(3-dimethyl aminopropyl) carbodiimide; beta-Lactams; Bromosuccinimide; Carboxyphenanthroline; Cholate; Cu2+; Deoxycholate; Diethyl dicarbonate; Dinitrophenol; Hg2+; Mg2+ most serine peptidases; NEM Cys; Phenyl glyoxal; SDS; Sodiumchloride [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA