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The map label for this gene is folD

Identifier: 15594372

GI number: 15594372

Start: 24697

End: 25623

Strand: Reverse

Name: folD

Synonym: BB0026

Alternate gene names: 15594372

Gene position: 25623-24697 (Counterclockwise)

Preceding gene: 15594375

Following gene: 15594371

Centisome position: 2.81

GC content: 32.15

Gene sequence:

>927_bases
TTGGGGTTTAAGGGGTATATTTTGAGTATTGTGTTTAATGGGAAAGATTTTGCGAATAAGTATTATTTAATGCTCAAAGA
ATTCTTAAAACAGCATAACTTGAGAGATAAAATTGCATTAAAAGTTGTTTTAGCAAATGATGAGCCTGCAAGCAATCTTT
ATGTTTCAATTAAGAGTCGAGTTGCAAAAGAGATTGGCTTGAATGTTGAAGTAATTAAATTTTCTGCAAACTCTGTTCAA
AGCGATATTTTAGAAGTAATTGATAGAGAAAATAAAAATTTAAGTACAGATGGAATTATTGTGCAATTACCCCTTTTGAA
AGGTATGGATTTAAATTCTATTTTAAATAGCATAGTTTATTCAAAAGATGTTGATGGCTTATCTTTTGTTAATTTGGGAA
AAATGATTTTGGGCGATAAAAAAGGGTTTATTCCTTGTACAGCTCTTGCTGTATTAAAGATCTTGCGAGATGAAGGGATA
AAAACATTGGGAAAAACGGTTGTTGTGGTTGGTAGAAGTCCTCTTGTAGGAAGGCCTATTTCAATATTGCTTTCATCAAA
ACCTTACGATGCAACTGTTATTGTTTGTCACAGCAAGAGCATTTATTTAGATGTTTATTTAAGACAGGCAGATATTGTTA
TTTCTGCTGTTGGTAAGCCTAGATTAATAGATAAAAGCATGTTGTGCGGAAAGCCTTATGTGATTGATATTGGCATTTCT
GAAATTGAGACTGACAACGGGAAAATTCTCTCAGGCGATACAGATTTTGACAATATTAAAGAGTGCGTTAAATTTATTAC
TCCTGTTAAGGGGGGGATAGGTCCTGTTACGGTTCTTATGTTAATGTTTAATACAATTAAAGCTCATTTGATTAATAATA
GGATGTTTGACGTTTTAAATCGGTTGGAAAAATTGTTGGAGGTGTAA

Upstream 100 bases:

>100_bases
TTGTTTATTATAGTTTAAAATAAATTTAATTACAATTTGAAATTTGGTCGTTTGACTTTCCTTATATTGACAATCTAAGT
ATAATATTAAGGTAATCTTA

Downstream 100 bases:

>100_bases
ATGTCTGGTCACAGTAAATGGTCAACAATAAAGAGAAAAAAAGGTGCTCTTGATGCAAAGCGTAATAAAATTTTTACAAA
GCTAATAAGAGAAATAACTA

Product: methylenetetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MGFKGYILSIVFNGKDFANKYYLMLKEFLKQHNLRDKIALKVVLANDEPASNLYVSIKSRVAKEIGLNVEVIKFSANSVQ
SDILEVIDRENKNLSTDGIIVQLPLLKGMDLNSILNSIVYSKDVDGLSFVNLGKMILGDKKGFIPCTALAVLKILRDEGI
KTLGKTVVVVGRSPLVGRPISILLSSKPYDATVIVCHSKSIYLDVYLRQADIVISAVGKPRLIDKSMLCGKPYVIDIGIS
EIETDNGKILSGDTDFDNIKECVKFITPVKGGIGPVTVLMLMFNTIKAHLINNRMFDVLNRLEKLLEV

Sequences:

>Translated_308_residues
MGFKGYILSIVFNGKDFANKYYLMLKEFLKQHNLRDKIALKVVLANDEPASNLYVSIKSRVAKEIGLNVEVIKFSANSVQ
SDILEVIDRENKNLSTDGIIVQLPLLKGMDLNSILNSIVYSKDVDGLSFVNLGKMILGDKKGFIPCTALAVLKILRDEGI
KTLGKTVVVVGRSPLVGRPISILLSSKPYDATVIVCHSKSIYLDVYLRQADIVISAVGKPRLIDKSMLCGKPYVIDIGIS
EIETDNGKILSGDTDFDNIKECVKFITPVKGGIGPVTVLMLMFNTIKAHLINNRMFDVLNRLEKLLEV
>Mature_307_residues
GFKGYILSIVFNGKDFANKYYLMLKEFLKQHNLRDKIALKVVLANDEPASNLYVSIKSRVAKEIGLNVEVIKFSANSVQS
DILEVIDRENKNLSTDGIIVQLPLLKGMDLNSILNSIVYSKDVDGLSFVNLGKMILGDKKGFIPCTALAVLKILRDEGIK
TLGKTVVVVGRSPLVGRPISILLSSKPYDATVIVCHSKSIYLDVYLRQADIVISAVGKPRLIDKSMLCGKPYVIDIGISE
IETDNGKILSGDTDFDNIKECVKFITPVKGGIGPVTVLMLMFNTIKAHLINNRMFDVLNRLEKLLEV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=35.6401384083045, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI222418558, Length=259, Percent_Identity=42.0849420849421, Blast_Score=177, Evalue=8e-45,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=38.6440677966102, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI36796743, Length=197, Percent_Identity=29.4416243654822, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1786741, Length=252, Percent_Identity=36.9047619047619, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17568735, Length=264, Percent_Identity=34.8484848484849, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=37.0629370629371, Blast_Score=171, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6321643, Length=263, Percent_Identity=34.9809885931559, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6322933, Length=182, Percent_Identity=29.6703296703297, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136818, Length=302, Percent_Identity=38.4105960264901, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI17136816, Length=302, Percent_Identity=38.4105960264901, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI62472483, Length=255, Percent_Identity=36.078431372549, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45551871, Length=255, Percent_Identity=36.078431372549, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24645718, Length=255, Percent_Identity=36.078431372549, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17137370, Length=255, Percent_Identity=36.078431372549, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_BORBU (O51057)

Other databases:

- EMBL:   AE000783
- PIR:   B70103
- RefSeq:   NP_212160.1
- HSSP:   P11586
- ProteinModelPortal:   O51057
- SMR:   O51057
- EnsemblBacteria:   EBBORT00000008399
- GeneID:   1194862
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0026
- NMPDR:   fig|224326.1.peg.410
- TIGR:   BB_0026
- GeneTree:   EBGT00050000007085
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- PhylomeDB:   O51057
- ProtClustDB:   CLSK507301
- BioCyc:   BBUR224326:BB_0026-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 34033; Mature: 33902

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKGYILSIVFNGKDFANKYYLMLKEFLKQHNLRDKIALKVVLANDEPASNLYVSIKSR
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEHHHH
VAKEIGLNVEVIKFSANSVQSDILEVIDRENKNLSTDGIIVQLPLLKGMDLNSILNSIVY
HHHHHCCCEEEEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
SKDVDGLSFVNLGKMILGDKKGFIPCTALAVLKILRDEGIKTLGKTVVVVGRSPLVGRPI
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCCCCCE
SILLSSKPYDATVIVCHSKSIYLDVYLRQADIVISAVGKPRLIDKSMLCGKPYVIDIGIS
EEEECCCCCCEEEEEEECCEEEEEEEEEEEEEEEEECCCCCEECCHHHCCCCEEEEECHH
EIETDNGKILSGDTDFDNIKECVKFITPVKGGIGPVTVLMLMFNTIKAHLINNRMFDVLN
HEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLEKLLEV
HHHHHHCC
>Mature Secondary Structure 
GFKGYILSIVFNGKDFANKYYLMLKEFLKQHNLRDKIALKVVLANDEPASNLYVSIKSR
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEHHHH
VAKEIGLNVEVIKFSANSVQSDILEVIDRENKNLSTDGIIVQLPLLKGMDLNSILNSIVY
HHHHHCCCEEEEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
SKDVDGLSFVNLGKMILGDKKGFIPCTALAVLKILRDEGIKTLGKTVVVVGRSPLVGRPI
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCCCCCE
SILLSSKPYDATVIVCHSKSIYLDVYLRQADIVISAVGKPRLIDKSMLCGKPYVIDIGIS
EEEECCCCCCEEEEEEECCEEEEEEEEEEEEEEEEECCCCCEECCHHHCCCCEEEEECHH
EIETDNGKILSGDTDFDNIKECVKFITPVKGGIGPVTVLMLMFNTIKAHLINNRMFDVLN
HEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLEKLLEV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685