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The map label for this gene is truA [H]

Identifier: 15594358

GI number: 15594358

Start: 11421

End: 12161

Strand: Direct

Name: truA [H]

Synonym: BB0012

Alternate gene names: 15594358

Gene position: 11421-12161 (Clockwise)

Preceding gene: 15594357

Following gene: 15594359

Centisome position: 1.25

GC content: 27.13

Gene sequence:

>741_bases
ATGAAAAAAATATTAGCTGAAATCGCTTATGACGGATCCATATACCATGGATTTCAAATACAACCAACAAAGCCTACAGT
TCAAGGAGAAATTGAAAAAGCTCTAATGAAGATCAATAAAAAAAAAGTAAAAATTCATTCATCCGGAAGAACAGATAAAG
GCGTTCATGCAAAAAAGCAAATAATAACTTTTGATATAAAAATCAATATTCAGCTAAACAATCTAAAAAAAGCTTTAAAT
GCAATATTATTAAAAAACAGCATAAAAATACTAAAGCTCAGGTATGTAAAAAATTCATTTCATCCACGTTTTAGCGCTCA
AAAAAGAAAATATAGCTATTGTATATTAAACAGCGATAACTACTATCCCTGGGAAGGTTATCAAGCTCACTATGTAAATA
AAAAACTAAGCATAAGCAATTTAAACCAAATGGCCAAAACATTGATTGGAAAACATGATTTTACCACATTTTCATGCATA
AAAGATAAAAGCAAATCTAAATTTAGGCATATATATTTTGCCAAATTTAAAAAAAGAGGAAAATATATTATTTTTGAAAT
AATAGGATCTTCTTTTTTGTGGAAAATGGTAAGATCAATAATAGGCACAATGCTGGACATTGAAATAAAAAACGAATCAA
TTTCTACTTTTGAAACAATATTAAAATCAAAAAATAGAAACCTTGCAAGAACAACTGCACCTGCAAATGCTTTATTTTTA
GAAAGAGTTTACTATGAATAA

Upstream 100 bases:

>100_bases
AGGCATGGGTAAATTTTCAAAAGAAAAGCCTTCAATACTTTTAGATAAAATCAAAGAAGTTTACTTTAAAATATCAAAGG
AAATGAAAAATCTTAAATAA

Downstream 100 bases:

>100_bases
TAGCGTTTTAACTAAAAAACTTTTTTTACTTAAAATATTGACAAATAATATAGAAGGCAAAATTACCGAAAATTTTGAAG
AAATAGACAATGAAATTAAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKKILAEIAYDGSIYHGFQIQPTKPTVQGEIEKALMKINKKKVKIHSSGRTDKGVHAKKQIITFDIKINIQLNNLKKALN
AILLKNSIKILKLRYVKNSFHPRFSAQKRKYSYCILNSDNYYPWEGYQAHYVNKKLSISNLNQMAKTLIGKHDFTTFSCI
KDKSKSKFRHIYFAKFKKRGKYIIFEIIGSSFLWKMVRSIIGTMLDIEIKNESISTFETILKSKNRNLARTTAPANALFL
ERVYYE

Sequences:

>Translated_246_residues
MKKILAEIAYDGSIYHGFQIQPTKPTVQGEIEKALMKINKKKVKIHSSGRTDKGVHAKKQIITFDIKINIQLNNLKKALN
AILLKNSIKILKLRYVKNSFHPRFSAQKRKYSYCILNSDNYYPWEGYQAHYVNKKLSISNLNQMAKTLIGKHDFTTFSCI
KDKSKSKFRHIYFAKFKKRGKYIIFEIIGSSFLWKMVRSIIGTMLDIEIKNESISTFETILKSKNRNLARTTAPANALFL
ERVYYE
>Mature_246_residues
MKKILAEIAYDGSIYHGFQIQPTKPTVQGEIEKALMKINKKKVKIHSSGRTDKGVHAKKQIITFDIKINIQLNNLKKALN
AILLKNSIKILKLRYVKNSFHPRFSAQKRKYSYCILNSDNYYPWEGYQAHYVNKKLSISNLNQMAKTLIGKHDFTTFSCI
KDKSKSKFRHIYFAKFKKRGKYIIFEIIGSSFLWKMVRSIIGTMLDIEIKNESISTFETILKSKNRNLARTTAPANALFL
ERVYYE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=30.8641975308642, Blast_Score=138, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28657; Mature: 28657

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILAEIAYDGSIYHGFQIQPTKPTVQGEIEKALMKINKKKVKIHSSGRTDKGVHAKKQ
CHHHHHHHHCCCCEEECEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
IITFDIKINIQLNNLKKALNAILLKNSIKILKLRYVKNSFHPRFSAQKRKYSYCILNSDN
EEEEEEEEEEEEHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCCCEEEEEEECCCC
YYPWEGYQAHYVNKKLSISNLNQMAKTLIGKHDFTTFSCIKDKSKSKFRHIYFAKFKKRG
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCC
KYIIFEIIGSSFLWKMVRSIIGTMLDIEIKNESISTFETILKSKNRNLARTTAPANALFL
CEEEEEECCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEECCCCCHHHHH
ERVYYE
HHHHCC
>Mature Secondary Structure
MKKILAEIAYDGSIYHGFQIQPTKPTVQGEIEKALMKINKKKVKIHSSGRTDKGVHAKKQ
CHHHHHHHHCCCCEEECEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
IITFDIKINIQLNNLKKALNAILLKNSIKILKLRYVKNSFHPRFSAQKRKYSYCILNSDN
EEEEEEEEEEEEHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCCCEEEEEEECCCC
YYPWEGYQAHYVNKKLSISNLNQMAKTLIGKHDFTTFSCIKDKSKSKFRHIYFAKFKKRG
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCC
KYIIFEIIGSSFLWKMVRSIIGTMLDIEIKNESISTFETILKSKNRNLARTTAPANALFL
CEEEEEECCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEECCCCCHHHHH
ERVYYE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA