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The map label for this gene is nrsF [H]

Identifier: 154687170

GI number: 154687170

Start: 2868410

End: 2870059

Strand: Reverse

Name: nrsF [H]

Synonym: RBAM_027420

Alternate gene names: 154687170

Gene position: 2870059-2868410 (Counterclockwise)

Preceding gene: 154687171

Following gene: 154687169

Centisome position: 73.24

GC content: 31.88

Gene sequence:

>1650_bases
GTGTATAAAACAGTTTATGATTTTATAGTTGATGCTGAAAATCGAGATGATAAAGGAATTACATTTATAAATGGATCCGA
TAATGAAGTATATATTTCTTATGCTCAATTATTTGAAAAATCCAGAAAAGTATTAGGTGTTTTACAAAAAAAGGGTTTAA
GAAAGAAAGATGAACTAATTATACAAATTGAGGATAATGAACAATTTATTAAAGTTTTTTGGGCGTGCATATTAGGTGGA
ATCATACCTATTCCTTTAAGTGTGGGGTATAATGAAGAGCATAAACAAAAAATACTTCGTATATGGAGTATTCTTAATAA
TCCTTATTGTATTTCTGATCAAAGAACATTACATTCACTTGAGAAAGTTGCAGATGCAGAAACTTATTCATCAATTGTAA
AACAATCAATTTATTTAGATGAAATTAAGAATTCTAATGAAGTGGGTGAATTAATTTCCCCTAAACCAGAAGAAATTGCT
TTTATTCAATTCTCTTCTGGTACAACTGGAGATCCTAAAGGTGTAATATTAACACATAAAAATCTTATTACCAATATATC
AGCATTAAATGAGGCTTGGGAGACATCGAAATCCGATTCCTCACTAAGCTGGATGCCTTTAACTCATGATATGGGCCTCA
TTGCTATTCACTTAGCATCAACGTATAAAAAAATTCAACAATATATAATTCCAACCTCCGTTTTTATCCGAAGACCGACT
TTGTGGTTATTGAAAACACATCAACACCGTGTTACACAATTATACTCCCCTAACTTTGGATATAAGTTTTTGTTGGATAA
CTATAAAAAAAATCAAATCTATAACTGGGATTTAACTTGTGTTAGATTATTAGCTAATGGAGCAGAACCAATTTCTACTT
CTTTGTGCCAGAGATTTTTAGAAGAAATGAAACAATTCGGATTAAAATATGAAACCCTAAATACTGTATATGGTTTAGCT
GAGGCTACAGTTGCTGTTAGTGTCCCAAAATTAGGAAACTCTTTTGTTCCTGTAACCCTTAATAGGGAAGCTTTAGAAGT
AGGTCAAAAAATTCAAAAAACTGTTGAAGAAAAAAAGGGAATAACTTTTGTTGAAGTTGGCCCGGCTGTACGTCATTGTT
CTTTCCGTATATGTGATTCTAATAATTACGTTCTGGATGATGATTTTATAGGTCATGTGCAAATAAAGGGGGAAAACGTA
ACGTCTGGGTATTACAATAATGAATCTGCTACCAAAGACGCTATAACTGCAGATGGATGGTTAAATACAGGAGACCTTGG
GTTTATACATAATAATAGTTTGGTTATTACCGGAAGAACAAAGGATATCATTATAAAGAATGGCCAAAATTATTATTCAC
ATGATATAGAGAGAGTAGCAGAGAATGTAAAGGATATTGAATTAGGTAATGTTGTGGCATGCGGGGTCCCGAATGAAACA
AAGGCGGAAGAAGATCTTGTGATATTTGTAAAATGTAAGCAAAGTTACGATATTACAACAGCAGAGAAGGAATTGAAAAG
AGAATTAACAAAACAATTGGGTTTGAATGTACATCAAGTTATACCTGTTAGGAAAATTTTCAAAACTACAAGCGGTAAAG
TGCAACGTTCTAAAATGAAGGATTTTTATAAAGAGTTAATTCTGTCTTGA

Upstream 100 bases:

>100_bases
ATACTATTATCTTAATTCTTCTAAATTTCATTCTCTTCTAAATGGATTATTAGGGAAATTTAAGTCTGCTTTAATTTTAG
AGAAAGAGGAGTAAAATTAA

Downstream 100 bases:

>100_bases
TTAAGTTTTACATTTAATCGTATTTTATACCATCTCTGTCTTGTGTATGAATTACCTGATATTTCTTGTGAGAATTTATT
TGGAATAAGAAATACTATTG

Product: NrsF

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MYKTVYDFIVDAENRDDKGITFINGSDNEVYISYAQLFEKSRKVLGVLQKKGLRKKDELIIQIEDNEQFIKVFWACILGG
IIPIPLSVGYNEEHKQKILRIWSILNNPYCISDQRTLHSLEKVADAETYSSIVKQSIYLDEIKNSNEVGELISPKPEEIA
FIQFSSGTTGDPKGVILTHKNLITNISALNEAWETSKSDSSLSWMPLTHDMGLIAIHLASTYKKIQQYIIPTSVFIRRPT
LWLLKTHQHRVTQLYSPNFGYKFLLDNYKKNQIYNWDLTCVRLLANGAEPISTSLCQRFLEEMKQFGLKYETLNTVYGLA
EATVAVSVPKLGNSFVPVTLNREALEVGQKIQKTVEEKKGITFVEVGPAVRHCSFRICDSNNYVLDDDFIGHVQIKGENV
TSGYYNNESATKDAITADGWLNTGDLGFIHNNSLVITGRTKDIIIKNGQNYYSHDIERVAENVKDIELGNVVACGVPNET
KAEEDLVIFVKCKQSYDITTAEKELKRELTKQLGLNVHQVIPVRKIFKTTSGKVQRSKMKDFYKELILS

Sequences:

>Translated_549_residues
MYKTVYDFIVDAENRDDKGITFINGSDNEVYISYAQLFEKSRKVLGVLQKKGLRKKDELIIQIEDNEQFIKVFWACILGG
IIPIPLSVGYNEEHKQKILRIWSILNNPYCISDQRTLHSLEKVADAETYSSIVKQSIYLDEIKNSNEVGELISPKPEEIA
FIQFSSGTTGDPKGVILTHKNLITNISALNEAWETSKSDSSLSWMPLTHDMGLIAIHLASTYKKIQQYIIPTSVFIRRPT
LWLLKTHQHRVTQLYSPNFGYKFLLDNYKKNQIYNWDLTCVRLLANGAEPISTSLCQRFLEEMKQFGLKYETLNTVYGLA
EATVAVSVPKLGNSFVPVTLNREALEVGQKIQKTVEEKKGITFVEVGPAVRHCSFRICDSNNYVLDDDFIGHVQIKGENV
TSGYYNNESATKDAITADGWLNTGDLGFIHNNSLVITGRTKDIIIKNGQNYYSHDIERVAENVKDIELGNVVACGVPNET
KAEEDLVIFVKCKQSYDITTAEKELKRELTKQLGLNVHQVIPVRKIFKTTSGKVQRSKMKDFYKELILS
>Mature_549_residues
MYKTVYDFIVDAENRDDKGITFINGSDNEVYISYAQLFEKSRKVLGVLQKKGLRKKDELIIQIEDNEQFIKVFWACILGG
IIPIPLSVGYNEEHKQKILRIWSILNNPYCISDQRTLHSLEKVADAETYSSIVKQSIYLDEIKNSNEVGELISPKPEEIA
FIQFSSGTTGDPKGVILTHKNLITNISALNEAWETSKSDSSLSWMPLTHDMGLIAIHLASTYKKIQQYIIPTSVFIRRPT
LWLLKTHQHRVTQLYSPNFGYKFLLDNYKKNQIYNWDLTCVRLLANGAEPISTSLCQRFLEEMKQFGLKYETLNTVYGLA
EATVAVSVPKLGNSFVPVTLNREALEVGQKIQKTVEEKKGITFVEVGPAVRHCSFRICDSNNYVLDDDFIGHVQIKGENV
TSGYYNNESATKDAITADGWLNTGDLGFIHNNSLVITGRTKDIIIKNGQNYYSHDIERVAENVKDIELGNVVACGVPNET
KAEEDLVIFVKCKQSYDITTAEKELKRELTKQLGLNVHQVIPVRKIFKTTSGKVQRSKMKDFYKELILS

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=577, Percent_Identity=21.6637781629116, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI44888818, Length=574, Percent_Identity=22.4738675958188, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI187761345, Length=544, Percent_Identity=23.1617647058824, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI187761343, Length=544, Percent_Identity=23.1617647058824, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI225735629, Length=581, Percent_Identity=21.6867469879518, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI45827692, Length=581, Percent_Identity=21.6867469879518, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI225735625, Length=581, Percent_Identity=21.6867469879518, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI225735627, Length=494, Percent_Identity=21.8623481781377, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI45827694, Length=494, Percent_Identity=21.8623481781377, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI45827696, Length=494, Percent_Identity=21.8623481781377, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI83745141, Length=537, Percent_Identity=23.8361266294227, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI45827698, Length=459, Percent_Identity=22.2222222222222, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI42794760, Length=238, Percent_Identity=24.7899159663866, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI42794758, Length=238, Percent_Identity=24.7899159663866, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI42794756, Length=238, Percent_Identity=24.7899159663866, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788107, Length=583, Percent_Identity=22.8130360205832, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693145, Length=542, Percent_Identity=23.8007380073801, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=557, Percent_Identity=23.8779174147217, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71994694, Length=401, Percent_Identity=26.4339152119701, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=401, Percent_Identity=26.4339152119701, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=401, Percent_Identity=26.4339152119701, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=23.2931726907631, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=561, Percent_Identity=20.4991087344029, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=415, Percent_Identity=26.7469879518072, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=412, Percent_Identity=26.9417475728155, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI193202679, Length=583, Percent_Identity=19.7255574614065, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=517, Percent_Identity=24.1779497098646, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24581924, Length=395, Percent_Identity=26.5822784810127, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI18859661, Length=543, Percent_Identity=22.8360957642726, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648257, Length=565, Percent_Identity=22.4778761061947, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653035, Length=542, Percent_Identity=21.7712177121771, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24654656, Length=344, Percent_Identity=21.5116279069767, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62321; Mature: 62321

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKTVYDFIVDAENRDDKGITFINGSDNEVYISYAQLFEKSRKVLGVLQKKGLRKKDELI
CCCEEEEHEEECCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEE
IQIEDNEQFIKVFWACILGGIIPIPLSVGYNEEHKQKILRIWSILNNPYCISDQRTLHSL
EEECCCHHHHHHHHHHHHCCCEECEEECCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHH
EKVADAETYSSIVKQSIYLDEIKNSNEVGELISPKPEEIAFIQFSSGTTGDPKGVILTHK
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCEEEEECH
NLITNISALNEAWETSKSDSSLSWMPLTHDMGLIAIHLASTYKKIQQYIIPTSVFIRRPT
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHCCCHHEEECCE
LWLLKTHQHRVTQLYSPNFGYKFLLDNYKKNQIYNWDLTCVRLLANGAEPISTSLCQRFL
EEEEECCHHHHHEECCCCCCEEEEECCCCCCEEEECCHHHHHHHHCCCCHHHHHHHHHHH
EEMKQFGLKYETLNTVYGLAEATVAVSVPKLGNSFVPVTLNREALEVGQKIQKTVEEKKG
HHHHHHCCEEHHHHHHHHHHHHEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
ITFVEVGPAVRHCSFRICDSNNYVLDDDFIGHVQIKGENVTSGYYNNESATKDAITADGW
CEEEEECCHHHCCEEEEECCCCEEECCCCEEEEEEECCCEECCEECCCCCCCCCEEECCC
LNTGDLGFIHNNSLVITGRTKDIIIKNGQNYYSHDIERVAENVKDIELGNVVACGVPNET
CCCCCEEEEECCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCEECCCEEEECCCCCC
KAEEDLVIFVKCKQSYDITTAEKELKRELTKQLGLNVHQVIPVRKIFKTTSGKVQRSKMK
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
DFYKELILS
HHHHHHHCC
>Mature Secondary Structure
MYKTVYDFIVDAENRDDKGITFINGSDNEVYISYAQLFEKSRKVLGVLQKKGLRKKDELI
CCCEEEEHEEECCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEE
IQIEDNEQFIKVFWACILGGIIPIPLSVGYNEEHKQKILRIWSILNNPYCISDQRTLHSL
EEECCCHHHHHHHHHHHHCCCEECEEECCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHH
EKVADAETYSSIVKQSIYLDEIKNSNEVGELISPKPEEIAFIQFSSGTTGDPKGVILTHK
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCEEEEECH
NLITNISALNEAWETSKSDSSLSWMPLTHDMGLIAIHLASTYKKIQQYIIPTSVFIRRPT
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHCCCHHEEECCE
LWLLKTHQHRVTQLYSPNFGYKFLLDNYKKNQIYNWDLTCVRLLANGAEPISTSLCQRFL
EEEEECCHHHHHEECCCCCCEEEEECCCCCCEEEECCHHHHHHHHCCCCHHHHHHHHHHH
EEMKQFGLKYETLNTVYGLAEATVAVSVPKLGNSFVPVTLNREALEVGQKIQKTVEEKKG
HHHHHHCCEEHHHHHHHHHHHHEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
ITFVEVGPAVRHCSFRICDSNNYVLDDDFIGHVQIKGENVTSGYYNNESATKDAITADGW
CEEEEECCHHHCCEEEEECCCCEEECCCCEEEEEEECCCEECCEECCCCCCCCCEEECCC
LNTGDLGFIHNNSLVITGRTKDIIIKNGQNYYSHDIERVAENVKDIELGNVVACGVPNET
CCCCCEEEEECCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCEECCCEEEECCCCCC
KAEEDLVIFVKCKQSYDITTAEKELKRELTKQLGLNVHQVIPVRKIFKTTSGKVQRSKMK
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
DFYKELILS
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]