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The map label for this gene is grpE

Identifier: 154686808

GI number: 154686808

Start: 2497484

End: 2498059

Strand: Reverse

Name: grpE

Synonym: RBAM_023780

Alternate gene names: 154686808

Gene position: 2498059-2497484 (Counterclockwise)

Preceding gene: 154686809

Following gene: 154686807

Centisome position: 63.75

GC content: 46.01

Gene sequence:

>576_bases
ATGTCAGAAGAAAAACAAACCGCAGAACAGGTTGAAGCAGCAGAACAAGAAGAAGTGACAGAACAGGCAGAACAAGCCGC
TTCACAAGAGCAGCATGAAGAAACGGCAGGTCAGGAAGAAGCCCTTCAGCACCAAATTGATGAATTGCAGGGTTTGCTTG
ATGAAAAAGAAAACAAGCTTTTGCGTGTTCAAGCAGACTTTGAAAACTATAAACGACGCAGCCGCCTTGAAATGGAAGCT
GCTCAGAAATACCGTTCTCAAAACGTCGTAACGGAAATCCTTCCGGCTCTCGACAACTTTGAACGGGCGTTACAGGTAGA
AGCTGAAAGCGAGCAGACAAAAAGCTTGCTGCAAGGTATGGAAATGGTCCGCCGCCAGCTGATGGACGCACTTGAAAAAG
AAGGCGTTGAAGCCATCGAAGCTGTCGGTCAGGAATTTGATCCGAACCTTCACCAAGCCGTCATGCAGGTGGAAGATGAA
AACTTCGGCAGCAATATTGTTATCGAAGAACTGCAAAAAGGCTACAAACTGAAGGATCGCGTCATTCGTCCTTCAATGGT
AAAAGTGAATCAATAA

Upstream 100 bases:

>100_bases
CGGACTTGTCAAAAGCATTCACAAGTTTGTATGATGAATAAGGGAATTTTGGCAATCATTTTCAGTCATCTTTTTACAGC
ATTTGAGGGAGGTGAACACA

Downstream 100 bases:

>100_bases
CTACATAGCAGGAGGTAATTCAAAGTGAGTAAAGTAATCGGAATTGACTTGGGTACGACAAACTCATGTGTGGCAGTGCT
TGAAGGCGGAGAGCCTACAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MSEEKQTAEQVEAAEQEEVTEQAEQAASQEQHEETAGQEEALQHQIDELQGLLDEKENKLLRVQADFENYKRRSRLEMEA
AQKYRSQNVVTEILPALDNFERALQVEAESEQTKSLLQGMEMVRRQLMDALEKEGVEAIEAVGQEFDPNLHQAVMQVEDE
NFGSNIVIEELQKGYKLKDRVIRPSMVKVNQ

Sequences:

>Translated_191_residues
MSEEKQTAEQVEAAEQEEVTEQAEQAASQEQHEETAGQEEALQHQIDELQGLLDEKENKLLRVQADFENYKRRSRLEMEA
AQKYRSQNVVTEILPALDNFERALQVEAESEQTKSLLQGMEMVRRQLMDALEKEGVEAIEAVGQEFDPNLHQAVMQVEDE
NFGSNIVIEELQKGYKLKDRVIRPSMVKVNQ
>Mature_190_residues
SEEKQTAEQVEAAEQEEVTEQAEQAASQEQHEETAGQEEALQHQIDELQGLLDEKENKLLRVQADFENYKRRSRLEMEAA
QKYRSQNVVTEILPALDNFERALQVEAESEQTKSLLQGMEMVRRQLMDALEKEGVEAIEAVGQEFDPNLHQAVMQVEDEN
FGSNIVIEELQKGYKLKDRVIRPSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=174, Percent_Identity=29.3103448275862, Blast_Score=67, Evalue=8e-12,
Organism=Escherichia coli, GI1788967, Length=189, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17552458, Length=195, Percent_Identity=27.1794871794872, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=140, Percent_Identity=32.1428571428571, Blast_Score=82, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24653432, Length=139, Percent_Identity=31.6546762589928, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BACA2 (A7Z6W2)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421969.1
- ProteinModelPortal:   A7Z6W2
- SMR:   A7Z6W2
- STRING:   A7Z6W2
- EnsemblBacteria:   EBBACT00000005856
- GeneID:   5460812
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_023780
- eggNOG:   COG0576
- GeneTree:   EBGT00050000001213
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14140
- BioCyc:   BAMY326423:RBAM_023780-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21960; Mature: 21828

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKQTAEQVEAAEQEEVTEQAEQAASQEQHEETAGQEEALQHQIDELQGLLDEKENKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
LRVQADFENYKRRSRLEMEAAQKYRSQNVVTEILPALDNFERALQVEAESEQTKSLLQGM
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMVRRQLMDALEKEGVEAIEAVGQEFDPNLHQAVMQVEDENFGSNIVIEELQKGYKLKDR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHH
VIRPSMVKVNQ
HCCCCHHCCCC
>Mature Secondary Structure 
SEEKQTAEQVEAAEQEEVTEQAEQAASQEQHEETAGQEEALQHQIDELQGLLDEKENKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
LRVQADFENYKRRSRLEMEAAQKYRSQNVVTEILPALDNFERALQVEAESEQTKSLLQGM
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMVRRQLMDALEKEGVEAIEAVGQEFDPNLHQAVMQVEDENFGSNIVIEELQKGYKLKDR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHH
VIRPSMVKVNQ
HCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA