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The map label for this gene is yqfS

Identifier: 154686774

GI number: 154686774

Start: 2462913

End: 2463806

Strand: Reverse

Name: yqfS

Synonym: RBAM_023440

Alternate gene names: 154686774

Gene position: 2463806-2462913 (Counterclockwise)

Preceding gene: 154686775

Following gene: 154686772

Centisome position: 62.87

GC content: 47.2

Gene sequence:

>894_bases
TTGCTGAAAATAGGTTCACACGTCTCAATGAGCGGAAAACATATGCTCCTTGCCGCAAGCAAGGAAGCTGTTTCGTACGG
GGCGAACACATTTATGATCTATACGGGCGCTCCCCAAAACACGCGCCGCAAAAAAATTGAGGACTTGAATATTGAAGCGG
GCCGCGCCCATATGAAGGAAAACGGCATCGATGAGATTGTCGTGCATGCGCCGTACATCATTAACATTGGAAATACGACA
AATCCGGCAACATTTGAGCTGGGCGTTGATTTTCTGCGTTCTGAAATTGAAAGAACGGCAGCGATCGGCGCGAGACAGAT
CGTGCTTCATCCAGGGGCGCATGTCGGAGCCGGGGCTGAGACGGGCATCAAAAAAATCATTGAAGGTTTAAACGAAGTGA
TTGTTCCCGGGCAAAACGTGCAGATTGCGCTGGAGACAATGGCGGGCAAAGGATCAGAATGCGGAAGAAGCTTTGAAGAG
CTGGCCGAGATCATTGCGGGAGTCACGCATAATGAACATCTATCCGTCTGCTTTGACACTTGCCACACCCATGACGCCGG
GTATGATGTCGCTAATGATTTTGACGGTGTTCTTAACGAGTTTGATAAAATCGTCGGCATCGATCGGATAAAAGTCCTTC
ATGTTAACGACAGTAAAAATATTAAAGGCGCCCGGAAAGACCGCCATGAAAATATCGGGTTCGGAGAGATCGGTTTTGAC
GCTCTGCAGTACATCGTTCATCACGAGCAGCTTGCCGATATTCCGAAAATCCTCGAAACACCGTATGTAGGCGAAGACAA
GAAAAATAAGAAGCCGCCGTACCGTTTTGAAATCGAAATGCTGAAAGAAAAGCAGTTTGATAAGGAGCTTCTTGAAAAAA
TATCAGCTCAATAA

Upstream 100 bases:

>100_bases
TAAAGCCGGGCTATAAAAAGAAAATGAGCTACGAAATGGAAAAAATTAAAAAGAAGCAGAGAAGAAACCAATCTAACAGA
GGAAAGTAGGGGGAATCGGG

Downstream 100 bases:

>100_bases
GAAAGAAAGGAGGGGCGGGTGTCCCTCCTTTCTTACCCTGAAAATTGATTGAAAAGTTCGTCAACTTTCCGCGCCGTTTC
TTTTGAGGTTATCGTTTCCA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLKIGSHVSMSGKHMLLAASKEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMKENGIDEIVVHAPYIINIGNTT
NPATFELGVDFLRSEIERTAAIGARQIVLHPGAHVGAGAETGIKKIIEGLNEVIVPGQNVQIALETMAGKGSECGRSFEE
LAEIIAGVTHNEHLSVCFDTCHTHDAGYDVANDFDGVLNEFDKIVGIDRIKVLHVNDSKNIKGARKDRHENIGFGEIGFD
ALQYIVHHEQLADIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDKELLEKISAQ

Sequences:

>Translated_297_residues
MLKIGSHVSMSGKHMLLAASKEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMKENGIDEIVVHAPYIINIGNTT
NPATFELGVDFLRSEIERTAAIGARQIVLHPGAHVGAGAETGIKKIIEGLNEVIVPGQNVQIALETMAGKGSECGRSFEE
LAEIIAGVTHNEHLSVCFDTCHTHDAGYDVANDFDGVLNEFDKIVGIDRIKVLHVNDSKNIKGARKDRHENIGFGEIGFD
ALQYIVHHEQLADIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDKELLEKISAQ
>Mature_297_residues
MLKIGSHVSMSGKHMLLAASKEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMKENGIDEIVVHAPYIINIGNTT
NPATFELGVDFLRSEIERTAAIGARQIVLHPGAHVGAGAETGIKKIIEGLNEVIVPGQNVQIALETMAGKGSECGRSFEE
LAEIIAGVTHNEHLSVCFDTCHTHDAGYDVANDFDGVLNEFDKIVGIDRIKVLHVNDSKNIKGARKDRHENIGFGEIGFD
ALQYIVHHEQLADIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDKELLEKISAQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=295, Percent_Identity=31.864406779661, Blast_Score=138, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17531193, Length=263, Percent_Identity=33.0798479087452, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322735, Length=297, Percent_Identity=30.6397306397306, Blast_Score=145, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_BACA2 (A7Z6S8)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421935.1
- ProteinModelPortal:   A7Z6S8
- SMR:   A7Z6S8
- STRING:   A7Z6S8
- EnsemblBacteria:   EBBACT00000005875
- GeneID:   5461457
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_023440
- eggNOG:   COG0648
- GeneTree:   EBGT00050000001754
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   BAMY326423:RBAM_023440-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 32793; Mature: 32793

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIGSHVSMSGKHMLLAASKEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMKE
CCCCCCCCCCCCCEEEEEECHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHH
NGIDEIVVHAPYIINIGNTTNPATFELGVDFLRSEIERTAAIGARQIVLHPGAHVGAGAE
CCCCEEEEECCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHH
TGIKKIIEGLNEVIVPGQNVQIALETMAGKGSECGRSFEELAEIIAGVTHNEHLSVCFDT
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
CHTHDAGYDVANDFDGVLNEFDKIVGIDRIKVLHVNDSKNIKGARKDRHENIGFGEIGFD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCCHHHHH
ALQYIVHHEQLADIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDKELLEKISAQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKIGSHVSMSGKHMLLAASKEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMKE
CCCCCCCCCCCCCEEEEEECHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHH
NGIDEIVVHAPYIINIGNTTNPATFELGVDFLRSEIERTAAIGARQIVLHPGAHVGAGAE
CCCCEEEEECCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHH
TGIKKIIEGLNEVIVPGQNVQIALETMAGKGSECGRSFEELAEIIAGVTHNEHLSVCFDT
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
CHTHDAGYDVANDFDGVLNEFDKIVGIDRIKVLHVNDSKNIKGARKDRHENIGFGEIGFD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCCHHHHH
ALQYIVHHEQLADIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDKELLEKISAQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA