Click here to switch to the map view.

The map label for this gene is gcvT

Identifier: 154686720

GI number: 154686720

Start: 2421651

End: 2422751

Strand: Reverse

Name: gcvT

Synonym: RBAM_022890

Alternate gene names: 154686720

Gene position: 2422751-2421651 (Counterclockwise)

Preceding gene: 154686725

Following gene: 154686719

Centisome position: 61.83

GC content: 47.23

Gene sequence:

>1101_bases
ATGCTGAAACGAACACCGCTTTATGACGTGTATAAAGAATACGGCGGAAAAACGATTGATTTTGGAGGATGGGAGCTGCC
GGTTCAATTTTCTTCTATTAAAGAGGAGCATGAAGCCGTCCGTACAAAAGCGGGCCTGTTTGATGTGTCCCATATGGGGG
AAGTCGAAGTATCCGGTAAAGACGCCCTGTCTTTTCTGCAAAAAATGATGACAAATGACGTTGCCGATTTAAAGCCGGGA
AACGCGCTTTATACCGCCATGTGTTATCCTGACGGCGGTACGGTCGATGACTTGCTGATCTATCAGAAAAGCGAGAGCTG
CTATCTGCTCGTGATTAACGCGTCTAATATTGAAAAAGACATCGCTTGGCTGACGGAACATACGGAAGGCGACGTCACGC
TGACGAATCAGTCTGACGGGATTTCACTGCTTGCCGTTCAGGGGCCGAATGCTCAGTCGGTTCTTGCGAAGCTGACTGAA
TGTGATTTGTCCTCCTTAAAGCCGTTTACATTTATTGATAAGGCGGACGTTGCCGGACGCCAAGTCCTGCTTTCAAGAAC
AGGCTATACCGGTGAAGACGGATTTGAGTTATATTGCCGCAATGAAGATGCGGTTCATCTATTTAAGGAGATACTTGCAG
CGGGAGAGCATGAAGGCCTCGTTCCGTGCGGTCTCGGCGCCCGTGATACGCTGAGATTTGAAGCGAAGCTTGCTTTATAC
GGTCAGGAATTGACGAAAGATATTACACCGATTGAAGCCGGAATCGGATTTGCCGTGAAACACAAGAAAGATTCCGATTT
TTTCGGGAAATCAGTATTGCGCGAACAGAAAGAAAAAGGAGCCCCGCGAAAGCTGGTCGGACTTGAGATGATTGAAAAAG
GGATTCCGAGACACGGATACGCCGTGAAAAAAGACGGTGTTCCGATCGGTGAAGTCACGACAGGCACGCAATCTCCGACT
TTAAAGAAAAATATCGGCCTTGCCTTAATCAAAACGGAATTCAGTGAAGTCGGAACGGAAGTAGAAGTGGAAATCCGTAA
AAAAACGGTAAAAGCGAAGATTGTCCGCACACCGTTTTATAAACGTCCAAAACAGAGCTGA

Upstream 100 bases:

>100_bases
TTTCAGGTGCCGGGACAGAGAAACCTTCATTTTCATGAGGTGTTTCTCTGTCCTTTTTTATATGTTTTTACAATGCGCCA
ATGACTGAAGGGGGAAGATG

Downstream 100 bases:

>100_bases
AAAAGGAGAGTCATATGAAGCATCGATATTTGCCCGCGACAGAACAGGATAAAAAAGAAATGCTGAAAGCCATCGGCGCA
GAAACAATTGATGAACTGTT

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MLKRTPLYDVYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEVSGKDALSFLQKMMTNDVADLKPG
NALYTAMCYPDGGTVDDLLIYQKSESCYLLVINASNIEKDIAWLTEHTEGDVTLTNQSDGISLLAVQGPNAQSVLAKLTE
CDLSSLKPFTFIDKADVAGRQVLLSRTGYTGEDGFELYCRNEDAVHLFKEILAAGEHEGLVPCGLGARDTLRFEAKLALY
GQELTKDITPIEAGIGFAVKHKKDSDFFGKSVLREQKEKGAPRKLVGLEMIEKGIPRHGYAVKKDGVPIGEVTTGTQSPT
LKKNIGLALIKTEFSEVGTEVEVEIRKKTVKAKIVRTPFYKRPKQS

Sequences:

>Translated_366_residues
MLKRTPLYDVYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEVSGKDALSFLQKMMTNDVADLKPG
NALYTAMCYPDGGTVDDLLIYQKSESCYLLVINASNIEKDIAWLTEHTEGDVTLTNQSDGISLLAVQGPNAQSVLAKLTE
CDLSSLKPFTFIDKADVAGRQVLLSRTGYTGEDGFELYCRNEDAVHLFKEILAAGEHEGLVPCGLGARDTLRFEAKLALY
GQELTKDITPIEAGIGFAVKHKKDSDFFGKSVLREQKEKGAPRKLVGLEMIEKGIPRHGYAVKKDGVPIGEVTTGTQSPT
LKKNIGLALIKTEFSEVGTEVEVEIRKKTVKAKIVRTPFYKRPKQS
>Mature_366_residues
MLKRTPLYDVYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEVSGKDALSFLQKMMTNDVADLKPG
NALYTAMCYPDGGTVDDLLIYQKSESCYLLVINASNIEKDIAWLTEHTEGDVTLTNQSDGISLLAVQGPNAQSVLAKLTE
CDLSSLKPFTFIDKADVAGRQVLLSRTGYTGEDGFELYCRNEDAVHLFKEILAAGEHEGLVPCGLGARDTLRFEAKLALY
GQELTKDITPIEAGIGFAVKHKKDSDFFGKSVLREQKEKGAPRKLVGLEMIEKGIPRHGYAVKKDGVPIGEVTTGTQSPT
LKKNIGLALIKTEFSEVGTEVEVEIRKKTVKAKIVRTPFYKRPKQS

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=372, Percent_Identity=35.4838709677419, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI257796258, Length=358, Percent_Identity=35.4748603351955, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI257796254, Length=365, Percent_Identity=33.4246575342466, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI24797151, Length=336, Percent_Identity=32.4404761904762, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI257796256, Length=319, Percent_Identity=32.9153605015674, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI197927446, Length=353, Percent_Identity=27.1954674220963, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21361378, Length=353, Percent_Identity=27.1954674220963, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194306651, Length=375, Percent_Identity=25.0666666666667, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1789272, Length=374, Percent_Identity=39.0374331550802, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17560118, Length=374, Percent_Identity=36.096256684492, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32563613, Length=368, Percent_Identity=25.8152173913043, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994045, Length=376, Percent_Identity=26.8617021276596, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994052, Length=376, Percent_Identity=26.8617021276596, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320222, Length=388, Percent_Identity=34.5360824742268, Blast_Score=192, Evalue=9e-50,
Organism=Drosophila melanogaster, GI20129441, Length=384, Percent_Identity=35.15625, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28571104, Length=336, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20130091, Length=367, Percent_Identity=25.3405994550409, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_BACA2 (A7Z6M4)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421881.1
- ProteinModelPortal:   A7Z6M4
- SMR:   A7Z6M4
- STRING:   A7Z6M4
- EnsemblBacteria:   EBBACT00000005688
- GeneID:   5461362
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_022890
- eggNOG:   COG0404
- GeneTree:   EBGT00050000001932
- HOGENOM:   HBG299834
- OMA:   NDISKPP
- ProtClustDB:   PRK00389
- BioCyc:   BAMY326423:RBAM_022890-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 40318; Mature: 40318

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRTPLYDVYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEVSGK
CCCCCCHHHHHHHHCCCEEECCCEEEEEEHHHHHHHHHHHHHHHCEEECCCCCCEEECCH
DALSFLQKMMTNDVADLKPGNALYTAMCYPDGGTVDDLLIYQKSESCYLLVINASNIEKD
HHHHHHHHHHHCCHHHCCCCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCCHHH
IAWLTEHTEGDVTLTNQSDGISLLAVQGPNAQSVLAKLTECDLSSLKPFTFIDKADVAGR
HHHHHCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHCCHHHCCCEEEECCCCCCCC
QVLLSRTGYTGEDGFELYCRNEDAVHLFKEILAAGEHEGLVPCGLGARDTLRFEAKLALY
EEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEEEH
GQELTKDITPIEAGIGFAVKHKKDSDFFGKSVLREQKEKGAPRKLVGLEMIEKGIPRHGY
HHHHHCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
AVKKDGVPIGEVTTGTQSPTLKKNIGLALIKTEFSEVGTEVEVEIRKKTVKAKIVRTPFY
EEECCCCCCCCEECCCCCCCHHHCCCEEEEEECHHHCCCEEEEEEEHHHHEEHEEECCCC
KRPKQS
CCCCCC
>Mature Secondary Structure
MLKRTPLYDVYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEVSGK
CCCCCCHHHHHHHHCCCEEECCCEEEEEEHHHHHHHHHHHHHHHCEEECCCCCCEEECCH
DALSFLQKMMTNDVADLKPGNALYTAMCYPDGGTVDDLLIYQKSESCYLLVINASNIEKD
HHHHHHHHHHHCCHHHCCCCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCCHHH
IAWLTEHTEGDVTLTNQSDGISLLAVQGPNAQSVLAKLTECDLSSLKPFTFIDKADVAGR
HHHHHCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHCCHHHCCCEEEECCCCCCCC
QVLLSRTGYTGEDGFELYCRNEDAVHLFKEILAAGEHEGLVPCGLGARDTLRFEAKLALY
EEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEEEH
GQELTKDITPIEAGIGFAVKHKKDSDFFGKSVLREQKEKGAPRKLVGLEMIEKGIPRHGY
HHHHHCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
AVKKDGVPIGEVTTGTQSPTLKKNIGLALIKTEFSEVGTEVEVEIRKKTVKAKIVRTPFY
EEECCCCCCCCEECCCCCCCHHHCCCEEEEEECHHHCCCEEEEEEEHHHHEEHEEECCCC
KRPKQS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA