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The map label for this gene is dacB [H]

Identifier: 154686564

GI number: 154686564

Start: 2227956

End: 2229107

Strand: Reverse

Name: dacB [H]

Synonym: RBAM_021330

Alternate gene names: 154686564

Gene position: 2229107-2227956 (Counterclockwise)

Preceding gene: 154686565

Following gene: 154686563

Centisome position: 56.89

GC content: 44.7

Gene sequence:

>1152_bases
ATGCGCTTTCTCAAAAAAGCTGCCGTATTTCTTTTGACTTTTGTTATTCTTTTTTCCATTACAGACGGCAATACGGCACA
TGCCCAAATACATGTCAGCGCAAAAAGCGCGATTATAATCGACGGAGCATCTGGCAGGGTGTTATTCGCCAAGGATGAAC
ATCATAAGCGGCGGATTGCAAGCATTACGAAAATCATGACGGCGGTGCTGGCGATTGAATCAGGGAAGATGGAAGAGACC
GTGACGGTTTCCCCGAATGCCGTAAAAACGGAAGGCTCAGCCATTTATCTTCAGCCGGGACAAAAAGTAAAGCTGAAAGA
TCTCGTATACGGGCTTATGTTAAGGTCAGGAAATGACGCCGCTGCGGCAATAGCGGAATATGTCGGCGGAAGCCTTGACG
GTTTTGTATTTATGATGAACAAAAAAGCCGAGCAAATCGGGATGGAAAATACAAAATTTCAAAATCCGCACGGGCTGGAC
GACCATGAAAATTTATATTCAACGGCGTATGATATGGCGCTTTTGACTAAATACGCGATGAAAATCAAGGAATACCGGAC
CGTTTCAGGCACAAAAGTGTATAAGGCGGAAACGATGGACCGGGTATGGAAAAATAAAAACAAGCTTCTGACCGGGCTCT
ACCGTTACAGCACAGGCGGAAAAACAGGTTACACAAAAATCGCGAAACGGACGCTCGTCTCGACGGCCTCAAAAGACGGA
ACGGATCTCATCGCGGTCACTATAAATGATCCGGATGATTGGGATGATCATATGAATATGTTTAATTATGTCTTTGAGCA
CTATAAAACCTATGTCGTCGCTAAAAAAGGCGGCATCCCGAAGCTGAAAAAGACGTTTTACGGAAAGCGTGCTTTTATTG
CCCGGGATGTCAAATATTTGTTAAAAGAGGATGAAATAGACGATGTGAAAATCCGCACAACCTTAATAAAACCGAAAAAA
GCCTGGAAAAAGGACGCCTCTCTCATTCCTGATGTCGTCGGCCGGATGACTGTTATGCATGATGGGAAAGTGATCGCCAA
GATCCCCGTCTATTATGAAAATGAGCGCCATAAAAAACCGAAAAAGCATTTCATTGAGACGTTTACGAGCCTGTTTATGA
GCGCGGCGGGCGGTTCGTCATGGTCAATTTAA

Upstream 100 bases:

>100_bases
TTGGGAGAGTTTTTTTATGTGAAAGTGATGCAATTGGTATTCATAAGTCATGGACATGCGCATAAACTTGTACAAACCCA
CAACAAGGACGTGAGCATTC

Downstream 100 bases:

>100_bases
TCTGGGTATGTCTGACGGTAATCGGACTTGTCTTTGCGATGTTTAACGGTACCTTGCAGGATGTGAATGAAGCCGTTTTT
AAAGGAGCAAAAGAAGCGGT

Product: DacB

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MRFLKKAAVFLLTFVILFSITDGNTAHAQIHVSAKSAIIIDGASGRVLFAKDEHHKRRIASITKIMTAVLAIESGKMEET
VTVSPNAVKTEGSAIYLQPGQKVKLKDLVYGLMLRSGNDAAAAIAEYVGGSLDGFVFMMNKKAEQIGMENTKFQNPHGLD
DHENLYSTAYDMALLTKYAMKIKEYRTVSGTKVYKAETMDRVWKNKNKLLTGLYRYSTGGKTGYTKIAKRTLVSTASKDG
TDLIAVTINDPDDWDDHMNMFNYVFEHYKTYVVAKKGGIPKLKKTFYGKRAFIARDVKYLLKEDEIDDVKIRTTLIKPKK
AWKKDASLIPDVVGRMTVMHDGKVIAKIPVYYENERHKKPKKHFIETFTSLFMSAAGGSSWSI

Sequences:

>Translated_383_residues
MRFLKKAAVFLLTFVILFSITDGNTAHAQIHVSAKSAIIIDGASGRVLFAKDEHHKRRIASITKIMTAVLAIESGKMEET
VTVSPNAVKTEGSAIYLQPGQKVKLKDLVYGLMLRSGNDAAAAIAEYVGGSLDGFVFMMNKKAEQIGMENTKFQNPHGLD
DHENLYSTAYDMALLTKYAMKIKEYRTVSGTKVYKAETMDRVWKNKNKLLTGLYRYSTGGKTGYTKIAKRTLVSTASKDG
TDLIAVTINDPDDWDDHMNMFNYVFEHYKTYVVAKKGGIPKLKKTFYGKRAFIARDVKYLLKEDEIDDVKIRTTLIKPKK
AWKKDASLIPDVVGRMTVMHDGKVIAKIPVYYENERHKKPKKHFIETFTSLFMSAAGGSSWSI
>Mature_383_residues
MRFLKKAAVFLLTFVILFSITDGNTAHAQIHVSAKSAIIIDGASGRVLFAKDEHHKRRIASITKIMTAVLAIESGKMEET
VTVSPNAVKTEGSAIYLQPGQKVKLKDLVYGLMLRSGNDAAAAIAEYVGGSLDGFVFMMNKKAEQIGMENTKFQNPHGLD
DHENLYSTAYDMALLTKYAMKIKEYRTVSGTKVYKAETMDRVWKNKNKLLTGLYRYSTGGKTGYTKIAKRTLVSTASKDG
TDLIAVTINDPDDWDDHMNMFNYVFEHYKTYVVAKKGGIPKLKKTFYGKRAFIARDVKYLLKEDEIDDVKIRTTLIKPKK
AWKKDASLIPDVVGRMTVMHDGKVIAKIPVYYENERHKKPKKHFIETFTSLFMSAAGGSSWSI

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=372, Percent_Identity=29.3010752688172, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1786851, Length=234, Percent_Identity=35.8974358974359, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87082022, Length=333, Percent_Identity=27.6276276276276, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082054, Length=221, Percent_Identity=29.4117647058824, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42998; Mature: 42998

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLKKAAVFLLTFVILFSITDGNTAHAQIHVSAKSAIIIDGASGRVLFAKDEHHKRRIA
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEEECCCCCEEEECCHHHHHHHH
SITKIMTAVLAIESGKMEETVTVSPNAVKTEGSAIYLQPGQKVKLKDLVYGLMLRSGNDA
HHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCEEEECCCCCEEHHHHHHHHHHCCCCHH
AAAIAEYVGGSLDGFVFMMNKKAEQIGMENTKFQNPHGLDDHENLYSTAYDMALLTKYAM
HHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KIKEYRTVSGTKVYKAETMDRVWKNKNKLLTGLYRYSTGGKTGYTKIAKRTLVSTASKDG
HHHHHHCCCCCEEEHHHHHHHHHCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCC
TDLIAVTINDPDDWDDHMNMFNYVFEHYKTYVVAKKGGIPKLKKTFYGKRAFIARDVKYL
CEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCHHHHHHHHHHH
LKEDEIDDVKIRTTLIKPKKAWKKDASLIPDVVGRMTVMHDGKVIAKIPVYYENERHKKP
HCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCEEEEECCEEEEEECEEECCCCCCCH
KKHFIETFTSLFMSAAGGSSWSI
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRFLKKAAVFLLTFVILFSITDGNTAHAQIHVSAKSAIIIDGASGRVLFAKDEHHKRRIA
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEEECCCCCEEEECCHHHHHHHH
SITKIMTAVLAIESGKMEETVTVSPNAVKTEGSAIYLQPGQKVKLKDLVYGLMLRSGNDA
HHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCEEEECCCCCEEHHHHHHHHHHCCCCHH
AAAIAEYVGGSLDGFVFMMNKKAEQIGMENTKFQNPHGLDDHENLYSTAYDMALLTKYAM
HHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KIKEYRTVSGTKVYKAETMDRVWKNKNKLLTGLYRYSTGGKTGYTKIAKRTLVSTASKDG
HHHHHHCCCCCEEEHHHHHHHHHCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCC
TDLIAVTINDPDDWDDHMNMFNYVFEHYKTYVVAKKGGIPKLKKTFYGKRAFIARDVKYL
CEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCHHHHHHHHHHH
LKEDEIDDVKIRTTLIKPKKAWKKDASLIPDVVGRMTVMHDGKVIAKIPVYYENERHKKP
HCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCEEEEECCEEEEEECEEECCCCCCCH
KKHFIETFTSLFMSAAGGSSWSI
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]