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The map label for this gene is folE

Identifier: 154686526

GI number: 154686526

Start: 2191243

End: 2191815

Strand: Reverse

Name: folE

Synonym: RBAM_020940

Alternate gene names: 154686526

Gene position: 2191815-2191243 (Counterclockwise)

Preceding gene: 154686527

Following gene: 154686525

Centisome position: 55.93

GC content: 48.52

Gene sequence:

>573_bases
ATGAAAGAAGTTAATAAAGAACAGATTGAACAGGCGGTTCGCCAGATTTTAGAGGCGATTGGAGAAGACCCGGACCGGGA
AGGGCTTTTAGATACGCCGAAACGCGTGGCAAAAATGTACGCGGAAGTATTCTCGGGATTAAATGAAGACCCGAAAGAAC
ACTTTAAAACCGTTTTCGGAGAAGACCATGAGGAGCTTGTTCTTGTAAAAGATATCGCGTTTCATTCCATGTGTGAACAT
CACCTTGTTCCTTTTTACGGAAAAGCGCATATTGCCTACATTCCGCGCGGCGGAAAAGTGACGGGCCTGAGTAAATTGGC
GCGGGCGGTGGAAGCCGTCTCAAAACGCCCGCAGCTGCAGGAGCGCATCACTTCAACGATTGCAGAAAGCATCGTAGAGA
CCTTAGATCCCCACGGCGTCATGGTTGTCGTCGAAGCTGAGCATATGTGCATGACGATGAGGGGCGTAAGAAAGCCGGGT
GCAAAAACAGTGACTTCCGCTGTAAGAGGTATTTTTAAAGATGACGCAGCGGCGCGTTCCGAAGTGTTAGAACATATTAA
ACGTGCGGATTAA

Upstream 100 bases:

>100_bases
AAGCAGCCTCGGTCACCGGGCAGGACTTTCGTTTTGCTTTTGGCGCGGCAAATGTGCTAGAATCGAAATTAAATGTATTC
AATGGTGGAGGACACAGACA

Downstream 100 bases:

>100_bases
TAATGATAGAGGGTGCAGATATGAATTCAAAGCATTCAAGTGATTTTGTCGTCATTAAAGCGGTCGAGGACGGCGTGAAC
GTCATCGGTTTAACGAGAGG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MKEVNKEQIEQAVRQILEAIGEDPDREGLLDTPKRVAKMYAEVFSGLNEDPKEHFKTVFGEDHEELVLVKDIAFHSMCEH
HLVPFYGKAHIAYIPRGGKVTGLSKLARAVEAVSKRPQLQERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPG
AKTVTSAVRGIFKDDAAARSEVLEHIKRAD

Sequences:

>Translated_190_residues
MKEVNKEQIEQAVRQILEAIGEDPDREGLLDTPKRVAKMYAEVFSGLNEDPKEHFKTVFGEDHEELVLVKDIAFHSMCEH
HLVPFYGKAHIAYIPRGGKVTGLSKLARAVEAVSKRPQLQERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPG
AKTVTSAVRGIFKDDAAARSEVLEHIKRAD
>Mature_190_residues
MKEVNKEQIEQAVRQILEAIGEDPDREGLLDTPKRVAKMYAEVFSGLNEDPKEHFKTVFGEDHEELVLVKDIAFHSMCEH
HLVPFYGKAHIAYIPRGGKVTGLSKLARAVEAVSKRPQLQERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPG
AKTVTSAVRGIFKDDAAARSEVLEHIKRAD

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=193, Percent_Identity=50.7772020725389, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI4503949, Length=193, Percent_Identity=50.7772020725389, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI66932970, Length=162, Percent_Identity=48.1481481481481, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI66932972, Length=152, Percent_Identity=50.6578947368421, Blast_Score=137, Evalue=5e-33,
Organism=Escherichia coli, GI1788476, Length=190, Percent_Identity=37.3684210526316, Blast_Score=117, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17560486, Length=188, Percent_Identity=50, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321706, Length=169, Percent_Identity=50.2958579881657, Blast_Score=169, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24656782, Length=180, Percent_Identity=49.4444444444444, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24656796, Length=180, Percent_Identity=49.4444444444444, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24656791, Length=180, Percent_Identity=49.4444444444444, Blast_Score=167, Evalue=4e-42,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_BACA2 (A7Z630)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421687.1
- ProteinModelPortal:   A7Z630
- SMR:   A7Z630
- STRING:   A7Z630
- EnsemblBacteria:   EBBACT00000007584
- GeneID:   5462283
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_020940
- eggNOG:   COG0302
- GeneTree:   EBGT00050000002882
- HOGENOM:   HBG370191
- OMA:   MAPIIGR
- ProtClustDB:   PRK09347
- BioCyc:   BAMY326423:RBAM_020940-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 21211; Mature: 21211

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVNKEQIEQAVRQILEAIGEDPDREGLLDTPKRVAKMYAEVFSGLNEDPKEHFKTVFG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
EDHEELVLVKDIAFHSMCEHHLVPFYGKAHIAYIPRGGKVTGLSKLARAVEAVSKRPQLQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHH
ERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPGAKTVTSAVRGIFKDDAAARS
HHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EVLEHIKRAD
HHHHHHHCCC
>Mature Secondary Structure
MKEVNKEQIEQAVRQILEAIGEDPDREGLLDTPKRVAKMYAEVFSGLNEDPKEHFKTVFG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
EDHEELVLVKDIAFHSMCEHHLVPFYGKAHIAYIPRGGKVTGLSKLARAVEAVSKRPQLQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHH
ERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPGAKTVTSAVRGIFKDDAAARS
HHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EVLEHIKRAD
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA