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The map label for this gene is nth [H]

Identifier: 154686481

GI number: 154686481

Start: 2150112

End: 2150771

Strand: Reverse

Name: nth [H]

Synonym: RBAM_020490

Alternate gene names: 154686481

Gene position: 2150771-2150112 (Counterclockwise)

Preceding gene: 154686482

Following gene: 154686480

Centisome position: 54.89

GC content: 45.76

Gene sequence:

>660_bases
GTGTTAAATTTAAAGCAGATTGAATATTGTCTTGAAAAAATCGGCGATATGTTTCCGCACGCAGAATGTGAACTTGTCCA
TTCAAATCCTTTCGAATTGGTTGTGGCAGTCGCATTATCCGCGCAATGTACGGATGCTCTCGTCAACAGAGTGACAAAAA
CGCTATTTCAAAAATATAAACGGCCCGAAGATTATCTTGCCGTATCGCTGGAAGAACTCCAGCAGGATATTAAATCAATC
GGCCTGTACCGTAATAAAGCCAAAAATATTCAAAAGCTGAGCAAAATGATCATCGAAGAGTACGGCGGAGAAGTGCCGAA
AGACCGGGATGAGCTTGTGAATCTGCCGGGTGTCGGACGGAAAACGGCAAATGTGGTCGTGTCTGTTGCTTTCGGCGTAC
CTGCGATTGCAGTAGACACTCATGTGGAGCGGGTCTCTAAACGGCTCGGTATCTGCCGCTGGAAGGACTCTGTTTTGGAA
GTAGAGAAAACATTAATGAAAAAAGTGCCGAAAGAGGATTGGTCTGTTACGCATCACAGATTAATATTTTTCGGCAGGTA
TCATTGTAAAGCGCAATCTCCCCGCTGTGCGGAATGTCCTTTGCTTCCGCTGTGCAGAGAAGGGCAGAAGCGTGACAAAA
AAGGGCTGGTGAAACGGTGA

Upstream 100 bases:

>100_bases
CCGGCGTGTTCAAACAAAACAAAATGATCCGCAAAAAGAGTATACGAGGCAGGTGCCTTTTTACAATTGGCTGGAACAAT
AACATGAGAAGGGGAGAAAC

Downstream 100 bases:

>100_bases
CGGCGATTGACATTCTGGCAGGCTATGAAACATACCTGCGCAGCCTTACGGACTTGGAAAGCCTCGAGATCAAAACGGCG
CTGCGCCGCCATCCGCTGTA

Product: hypothetical protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MLNLKQIEYCLEKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYKRPEDYLAVSLEELQQDIKSI
GLYRNKAKNIQKLSKMIIEEYGGEVPKDRDELVNLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLE
VEKTLMKKVPKEDWSVTHHRLIFFGRYHCKAQSPRCAECPLLPLCREGQKRDKKGLVKR

Sequences:

>Translated_219_residues
MLNLKQIEYCLEKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYKRPEDYLAVSLEELQQDIKSI
GLYRNKAKNIQKLSKMIIEEYGGEVPKDRDELVNLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLE
VEKTLMKKVPKEDWSVTHHRLIFFGRYHCKAQSPRCAECPLLPLCREGQKRDKKGLVKR
>Mature_219_residues
MLNLKQIEYCLEKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYKRPEDYLAVSLEELQQDIKSI
GLYRNKAKNIQKLSKMIIEEYGGEVPKDRDELVNLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLE
VEKTLMKKVPKEDWSVTHHRLIFFGRYHCKAQSPRCAECPLLPLCREGQKRDKKGLVKR

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=184, Percent_Identity=33.1521739130435, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI115298648, Length=200, Percent_Identity=28, Blast_Score=81, Evalue=6e-16,
Organism=Homo sapiens, GI6912520, Length=200, Percent_Identity=28, Blast_Score=81, Evalue=6e-16,
Organism=Homo sapiens, GI190358497, Length=200, Percent_Identity=28, Blast_Score=81, Evalue=6e-16,
Organism=Homo sapiens, GI115298650, Length=200, Percent_Identity=28, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI115298654, Length=200, Percent_Identity=28, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI115298652, Length=200, Percent_Identity=28, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1787920, Length=202, Percent_Identity=47.029702970297, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1789331, Length=181, Percent_Identity=24.3093922651934, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=36.3128491620112, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319304, Length=178, Percent_Identity=26.9662921348315, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324530, Length=191, Percent_Identity=25.130890052356, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550361, Length=174, Percent_Identity=32.7586206896552, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24975; Mature: 24975

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLKQIEYCLEKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYK
CCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPEDYLAVSLEELQQDIKSIGLYRNKAKNIQKLSKMIIEEYGGEVPKDRDELVNLPGVGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
KTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLEVEKTLMKKVPKEDWSVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LIFFGRYHCKAQSPRCAECPLLPLCREGQKRDKKGLVKR
HEEEEEHCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCCC
>Mature Secondary Structure
MLNLKQIEYCLEKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYK
CCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPEDYLAVSLEELQQDIKSIGLYRNKAKNIQKLSKMIIEEYGGEVPKDRDELVNLPGVGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
KTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLEVEKTLMKKVPKEDWSVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LIFFGRYHCKAQSPRCAECPLLPLCREGQKRDKKGLVKR
HEEEEEHCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]