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The map label for this gene is ilvA [H]

Identifier: 154686424

GI number: 154686424

Start: 2099168

End: 2100436

Strand: Reverse

Name: ilvA [H]

Synonym: RBAM_019920

Alternate gene names: 154686424

Gene position: 2100436-2099168 (Counterclockwise)

Preceding gene: 154686427

Following gene: 154686423

Centisome position: 53.6

GC content: 46.57

Gene sequence:

>1269_bases
ATGAAACCGTTGCTTAAAGAAGACTCTCTCATCCAGGTAAAAGACATTTTAAAAGCGCACCAAAATGTAAAGGACGTTGT
GATACATACGCCTTTGCAGAGGAATGACAGACTTTCTGAGAGGTATGGCTGCAATATCTATTTGAAAAGAGAAGACCTGC
AGGTGGTCAGATCATTTAAGCTCAGAGGGGCTTATAATAAAATGAGGCAGCTCTCAAAAGAGCAGACTGAAAACGGAATT
GTATGCGCAAGTGCGGGAAACCATGCGCAGGGGGTTGCGTTTTCATGCAAACACCTCGGCATTCACGGCAAGATTTTTAT
GCCGTCAACCACACCGAGACAGAAAGTATCCCAAGTGGAACTGTTCGGAAAAGAATTTGTTGAAATCATATTGACGGGTG
ACACTTTCGATGATGCGTATAAGAGTTCTGCGGAATGCTGTGAAAAAGAAAACCGCACGTTTATTCATCCGTTTGACGAT
CCCGATGTCATGGCCGGACAGGGAACGGCTGCCATCGAAATTTTAAATGACATTGACGTTGAACCGCATTTTCTTTTTGC
AAGCGTAGGGGGAGGTGGACTCCTGTCCGGGCTCGGCACGTACATGAAAAACATCTCGCCGGATACAAAAATCATAGCGG
TAGAGCCGAAGGGCGCAGCCTCCTATTTTGAATCGAAAAAAGCCGGACAAGTGGTTGAACTTGAGAAAATCGATAAATTT
GTCGATGGGGCGGCCGTAAAAAAAATCGGCGCGGAAACCTTTCAAACGCTCGAAACCGTCGTCGATGACGTTGTGCTTGT
CCCGGAAGGAAAAGTGTGCAGCACGATTCTCGAATTATATAATCAATGCGCCATCGTAGCGGAGCCGGCGGGGGCATTGT
CCGTGTCAGCACTTGATTTGTATAAGGAAGAGATTAAAGGGAAAAACGTCATCTGCATGGTCAGCGGAGGGAATAACGAC
ATCGGCAGAATGCAGGAGATGAAGGAGCGCTCCATGATTTATGAGGGGCTGCAGCATTATTTTATCGTTAATTTCCCGCA
AAGGGCGGGAGCGCTTCGCGAATACCTAGATGAAGTGCTTGGGCCTGATGATGACATCACCCGGTTTGAATACACGAAAA
AAAATAACAAAAGCAGCGGCCCCGCATTGGTCGGCATTGAGCTTAAGCATCGGGAAGATTACGGCGCGCTCATTGCCCGG
ATGAATCAAAAAGGATTTCATTACGTAGAGGTGAATAAAGATCAGGATCTGTTCCACCTGTTAATCTGA

Upstream 100 bases:

>100_bases
TTAATTTTATTTACAAAATTAAAATAATTGTCTTATTATTTGAATATTCATGTTATAATGGCATATTAAATAGGGATTTA
AATAAGAAAGGACTCTGTAA

Downstream 100 bases:

>100_bases
CTTTTTTTAGAACGACGCGCAAAATGACAGACACGCTTTCTCAGATTGTATATAATAAAAAGAAAACAATCGAAAAAGAA
GGATTGATATCTTGTTACTC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKPLLKEDSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYGCNIYLKREDLQVVRSFKLRGAYNKMRQLSKEQTENGI
VCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVELFGKEFVEIILTGDTFDDAYKSSAECCEKENRTFIHPFDD
PDVMAGQGTAAIEILNDIDVEPHFLFASVGGGGLLSGLGTYMKNISPDTKIIAVEPKGAASYFESKKAGQVVELEKIDKF
VDGAAVKKIGAETFQTLETVVDDVVLVPEGKVCSTILELYNQCAIVAEPAGALSVSALDLYKEEIKGKNVICMVSGGNND
IGRMQEMKERSMIYEGLQHYFIVNFPQRAGALREYLDEVLGPDDDITRFEYTKKNNKSSGPALVGIELKHREDYGALIAR
MNQKGFHYVEVNKDQDLFHLLI

Sequences:

>Translated_422_residues
MKPLLKEDSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYGCNIYLKREDLQVVRSFKLRGAYNKMRQLSKEQTENGI
VCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVELFGKEFVEIILTGDTFDDAYKSSAECCEKENRTFIHPFDD
PDVMAGQGTAAIEILNDIDVEPHFLFASVGGGGLLSGLGTYMKNISPDTKIIAVEPKGAASYFESKKAGQVVELEKIDKF
VDGAAVKKIGAETFQTLETVVDDVVLVPEGKVCSTILELYNQCAIVAEPAGALSVSALDLYKEEIKGKNVICMVSGGNND
IGRMQEMKERSMIYEGLQHYFIVNFPQRAGALREYLDEVLGPDDDITRFEYTKKNNKSSGPALVGIELKHREDYGALIAR
MNQKGFHYVEVNKDQDLFHLLI
>Mature_422_residues
MKPLLKEDSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYGCNIYLKREDLQVVRSFKLRGAYNKMRQLSKEQTENGI
VCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVELFGKEFVEIILTGDTFDDAYKSSAECCEKENRTFIHPFDD
PDVMAGQGTAAIEILNDIDVEPHFLFASVGGGGLLSGLGTYMKNISPDTKIIAVEPKGAASYFESKKAGQVVELEKIDKF
VDGAAVKKIGAETFQTLETVVDDVVLVPEGKVCSTILELYNQCAIVAEPAGALSVSALDLYKEEIKGKNVICMVSGGNND
IGRMQEMKERSMIYEGLQHYFIVNFPQRAGALREYLDEVLGPDDDITRFEYTKKNNKSSGPALVGIELKHREDYGALIAR
MNQKGFHYVEVNKDQDLFHLLI

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=319, Percent_Identity=31.974921630094, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI33469958, Length=316, Percent_Identity=27.2151898734177, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI19923959, Length=310, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1790207, Length=389, Percent_Identity=36.2467866323907, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789505, Length=323, Percent_Identity=35.9133126934984, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71991565, Length=346, Percent_Identity=31.5028901734104, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17537387, Length=387, Percent_Identity=30.2325581395349, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17508781, Length=315, Percent_Identity=26.031746031746, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320930, Length=367, Percent_Identity=38.1471389645777, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6322631, Length=309, Percent_Identity=34.9514563106796, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6319788, Length=286, Percent_Identity=26.2237762237762, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355833, Length=345, Percent_Identity=31.304347826087, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24645328, Length=249, Percent_Identity=30.1204819277108, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 46931; Mature: 46931

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLLKEDSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYGCNIYLKREDLQVVRSFK
CCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEEEEEHHHHHHHHHHH
LRGAYNKMRQLSKEQTENGIVCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVE
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEHEECCCEEEEECCCCCHHHHHHHHH
LFGKEFVEIILTGDTFDDAYKSSAECCEKENRTFIHPFDDPDVMAGQGTAAIEILNDIDV
HHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEHHHCCC
EPHFLFASVGGGGLLSGLGTYMKNISPDTKIIAVEPKGAASYFESKKAGQVVELEKIDKF
CCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCEEEHHHHHHH
VDGAAVKKIGAETFQTLETVVDDVVLVPEGKVCSTILELYNQCAIVAEPAGALSVSALDL
HCCHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCEEEECCCCCEEHHHHHH
YKEEIKGKNVICMVSGGNNDIGRMQEMKERSMIYEGLQHYFIVNFPQRAGALREYLDEVL
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHC
GPDDDITRFEYTKKNNKSSGPALVGIELKHREDYGALIARMNQKGFHYVEVNKDQDLFHL
CCCCCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCHHHH
LI
CC
>Mature Secondary Structure
MKPLLKEDSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYGCNIYLKREDLQVVRSFK
CCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEEEEEHHHHHHHHHHH
LRGAYNKMRQLSKEQTENGIVCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVE
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEHEECCCEEEEECCCCCHHHHHHHHH
LFGKEFVEIILTGDTFDDAYKSSAECCEKENRTFIHPFDDPDVMAGQGTAAIEILNDIDV
HHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEHHHCCC
EPHFLFASVGGGGLLSGLGTYMKNISPDTKIIAVEPKGAASYFESKKAGQVVELEKIDKF
CCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCEEEHHHHHHH
VDGAAVKKIGAETFQTLETVVDDVVLVPEGKVCSTILELYNQCAIVAEPAGALSVSALDL
HCCHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCEEEECCCCCEEHHHHHH
YKEEIKGKNVICMVSGGNNDIGRMQEMKERSMIYEGLQHYFIVNFPQRAGALREYLDEVL
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHC
GPDDDITRFEYTKKNNKSSGPALVGIELKHREDYGALIARMNQKGFHYVEVNKDQDLFHL
CCCCCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCHHHH
LI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]