Click here to switch to the map view.

The map label for this gene is liaR [H]

Identifier: 154686409

GI number: 154686409

Start: 2086586

End: 2087164

Strand: Reverse

Name: liaR [H]

Synonym: RBAM_019770

Alternate gene names: 154686409

Gene position: 2087164-2086586 (Counterclockwise)

Preceding gene: 154686410

Following gene: 154686408

Centisome position: 53.26

GC content: 37.31

Gene sequence:

>579_bases
GTGATTAGGGTATTGTTGGTCGATGACCATACAATGATTCGAAAGGGTCTCCGTGTGCTTTTGGAAGGCTATCCTGAAAT
CAAAATTGTTGGGGAAAGCCATAATGGGAATGATGCGATCCTTAAGACAAGACAGTTGAAACCGGATGTGGTGTTGATGG
ATTTGTCGATGCCAACTGGATTGGATGGATTTACAGCAAGCAGTGAAATCCTTAAATTGAGTCTACCGGTTAAAATCATC
ATTTTAACTATGTATGATGAAGAGATATTTGTCCAAAAAGCAATCGAAGTAGGGGCTTATGGATACATTTTAAAGAACAG
CCATTACGAACTGCTTTATAAAGCGATAGTCGAGGTTTCCTTAGGGAAACTCTTTTACAAGACATCTGTATCACAAGAGA
TTCTCTCTCAATGGATGACATCAGAATCTAAAAAGATTTTTTCTGTTTTAACAGTTCGAGAAAAGGAGATTGTTAGATTA
ATAGTACTGGGCTATACAAATAAAGAGATTGCTAATGAGCTATCGATTAGCTTCAAAACCGTGGAAAACCATAAGACGAA
TATTATGCACAGAAGTTAG

Upstream 100 bases:

>100_bases
GAGAGAGTAGATCAAATGGAAGGAAGTCTTCGAATTCACTCCGTTCCTTATAAAGGTACGTCTATTAAAGTGGAAGTACA
GTATAAGGAGGGGAAGGAGA

Downstream 100 bases:

>100_bases
AACTGGATTCGAAACATGAATTGATTCAATATGCGATCAATAACAACTATCTGGATCTTACATTGTAACGCACTGTTCCA
TGAACTGCCTATCATAAACT

Product: putative two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MIRVLLVDDHTMIRKGLRVLLEGYPEIKIVGESHNGNDAILKTRQLKPDVVLMDLSMPTGLDGFTASSEILKLSLPVKII
ILTMYDEEIFVQKAIEVGAYGYILKNSHYELLYKAIVEVSLGKLFYKTSVSQEILSQWMTSESKKIFSVLTVREKEIVRL
IVLGYTNKEIANELSISFKTVENHKTNIMHRS

Sequences:

>Translated_192_residues
MIRVLLVDDHTMIRKGLRVLLEGYPEIKIVGESHNGNDAILKTRQLKPDVVLMDLSMPTGLDGFTASSEILKLSLPVKII
ILTMYDEEIFVQKAIEVGAYGYILKNSHYELLYKAIVEVSLGKLFYKTSVSQEILSQWMTSESKKIFSVLTVREKEIVRL
IVLGYTNKEIANELSISFKTVENHKTNIMHRS
>Mature_192_residues
MIRVLLVDDHTMIRKGLRVLLEGYPEIKIVGESHNGNDAILKTRQLKPDVVLMDLSMPTGLDGFTASSEILKLSLPVKII
ILTMYDEEIFVQKAIEVGAYGYILKNSHYELLYKAIVEVSLGKLFYKTSVSQEILSQWMTSESKKIFSVLTVREKEIVRL
IVLGYTNKEIANELSISFKTVENHKTNIMHRS

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=186, Percent_Identity=38.1720430107527, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI1788521, Length=189, Percent_Identity=35.978835978836, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1786747, Length=185, Percent_Identity=32.972972972973, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1787473, Length=183, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=3e-20,
Organism=Escherichia coli, GI1790102, Length=191, Percent_Identity=29.3193717277487, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788712, Length=190, Percent_Identity=27.3684210526316, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788546, Length=194, Percent_Identity=29.3814432989691, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI87082052, Length=105, Percent_Identity=38.0952380952381, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21860; Mature: 21860

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVLLVDDHTMIRKGLRVLLEGYPEIKIVGESHNGNDAILKTRQLKPDVVLMDLSMPTG
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEECCCCCCEEEEEECCCCC
LDGFTASSEILKLSLPVKIIILTMYDEEIFVQKAIEVGAYGYILKNSHYELLYKAIVEVS
CCCCCCCCCEEEEECCEEEEEEEEECHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHH
LGKLFYKTSVSQEILSQWMTSESKKIFSVLTVREKEIVRLIVLGYTNKEIANELSISFKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHEEEEEEEECCCHHHHHHHEEEEEE
VENHKTNIMHRS
ECCCCCCCEECC
>Mature Secondary Structure
MIRVLLVDDHTMIRKGLRVLLEGYPEIKIVGESHNGNDAILKTRQLKPDVVLMDLSMPTG
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEECCCCCCEEEEEECCCCC
LDGFTASSEILKLSLPVKIIILTMYDEEIFVQKAIEVGAYGYILKNSHYELLYKAIVEVS
CCCCCCCCCEEEEECCEEEEEEEEECHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHH
LGKLFYKTSVSQEILSQWMTSESKKIFSVLTVREKEIVRLIVLGYTNKEIANELSISFKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHEEEEEEEECCCHHHHHHHEEEEEE
VENHKTNIMHRS
ECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]