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The map label for this gene is yrkH [H]

Identifier: 154686407

GI number: 154686407

Start: 2083569

End: 2084981

Strand: Reverse

Name: yrkH [H]

Synonym: RBAM_019750

Alternate gene names: 154686407

Gene position: 2084981-2083569 (Counterclockwise)

Preceding gene: 154686408

Following gene: 154686406

Centisome position: 53.21

GC content: 43.95

Gene sequence:

>1413_bases
ATGTTATTGCGCTACTTTTACGATGAAAAGCTGGCCCATGCATCCTATCTTGTTGGTTGCCAGAAAACGGGAGACGCAAT
TGTGATTGATCCCAGCCGCAATATAAAACCATATTTGCAATTTGCAAAAGAAGAAGGACTAAACATCGTAGCGGCTGCAG
AAACTCATATTCATGCCGATTTTGTTTCCGGTGCTCGCGAAATCGGCGTTACGCATCAAGCAAAGCTGTTTCTTTCCGAT
GAAGGTGATGCAGATTGGAAGTATCAATATGTCGATGAAATAAAACATCAACTTGTGAAAGACGGTGATCGCTTTTCCAT
CGGAAAGTTGATTTTTGAGGTTATGCATACCCCCGGCCATACCCCGGAGAGCATTTCCTTTCTGTTGACGGATCGCGGAA
GTAATGCGGATCGTCCAATCGGCATCTTTACAGGAGATTTTGTCTTTGTAAGCGACGTCGGAAGACCCGACTTGCTGGAG
AAAGCGGCTGGCATCAAGGGTACGGCAGATTGTGGCGCAAGAGCAATGTTCAAGTCCCTATTGAAATTCAAACAACTGCC
AGATTATCTGCAGGTCTGGCCGGCTCATGGTGCAGGAAGCTCTTGTGGTAAGGCATTGGGGGCGATTCCTTCTTCCACTG
TTGGTTACGAAAAGCTATTCAACTGGTCAATGACTTACACCAACGAAAAAGATTTTGTAAAAGCCTTGCTTGAAGGGCAA
CCAGAACCGCCAAAATATTTCTCTGTGATGAAACAAATTAATAAAAAAGGCCCTGCGTTGCTTAAAGATTTACCTGTAGT
GTCAGAGATCACCTCAATGGAACAAGTGAGAGGCTTGCTGGATAGCGAACAAATTGTAGATACCCGTCCCTCTCAAGAAT
TTGCTAAAGGGCATGTGAAAGGTGCAATAAACATCCCATTTAACAAAGCTTTTTCTAACTGGGCGGGTTGGATTATTGAT
TACAATCGCCCTGTGTATTTTATTACGAACCCGCATGAAGCAGATAAACTTCTGAAAGCACTTCACTCCATAGGTATAGA
AAATGTGGCAGGATTCATGGACAGTGGAAAAATCATTGCAAGCGGTATGTTTTCGCTCGAATCATACACGGAGGTAACAT
CGTTAGAAATAGCAAATGCGGTGGAGAACGGACTCGTACAAGTGGTTGATGTTCGCAATCTGACTGAATGGCAAGAAGGT
CACATTCCGAACGCACAACATATCATGCTAGGAACGCTTCAAGGACGGCTAAATGAAATCAAAAGTGACAAACCGATTCT
TGTTCAATGTCTTTCCGGAGCTAGGTCTGCTATTGGTGCCAGCATCCTCCAAGCTAATAACGATTTTAAACAAGTATTGA
AATTGGATGGTGGAATTTCCCAGTGGCAGAAAGCTGGTCTTCCAATCGTGTGA

Upstream 100 bases:

>100_bases
ATAAAAAGTTCGAAAATCCTAAACATATTCCGCTTAAACTCGCTTCTCTATCCGATAAACTTTTTGTGGAATATGTAATG
ATGTAAGAGGAGGAAACAAC

Downstream 100 bases:

>100_bases
CAAGGACCAATCCCTTGTCTCTGTTCAAGTTTTCTCTTCAATGCAACTGAGTACACTAACTTATGTCAACTGTAGGGCAG
TTAGCTAAGTTCTCTTTAGA

Product: hypothetical protein

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLLRYFYDEKLAHASYLVGCQKTGDAIVIDPSRNIKPYLQFAKEEGLNIVAAAETHIHADFVSGAREIGVTHQAKLFLSD
EGDADWKYQYVDEIKHQLVKDGDRFSIGKLIFEVMHTPGHTPESISFLLTDRGSNADRPIGIFTGDFVFVSDVGRPDLLE
KAAGIKGTADCGARAMFKSLLKFKQLPDYLQVWPAHGAGSSCGKALGAIPSSTVGYEKLFNWSMTYTNEKDFVKALLEGQ
PEPPKYFSVMKQINKKGPALLKDLPVVSEITSMEQVRGLLDSEQIVDTRPSQEFAKGHVKGAINIPFNKAFSNWAGWIID
YNRPVYFITNPHEADKLLKALHSIGIENVAGFMDSGKIIASGMFSLESYTEVTSLEIANAVENGLVQVVDVRNLTEWQEG
HIPNAQHIMLGTLQGRLNEIKSDKPILVQCLSGARSAIGASILQANNDFKQVLKLDGGISQWQKAGLPIV

Sequences:

>Translated_470_residues
MLLRYFYDEKLAHASYLVGCQKTGDAIVIDPSRNIKPYLQFAKEEGLNIVAAAETHIHADFVSGAREIGVTHQAKLFLSD
EGDADWKYQYVDEIKHQLVKDGDRFSIGKLIFEVMHTPGHTPESISFLLTDRGSNADRPIGIFTGDFVFVSDVGRPDLLE
KAAGIKGTADCGARAMFKSLLKFKQLPDYLQVWPAHGAGSSCGKALGAIPSSTVGYEKLFNWSMTYTNEKDFVKALLEGQ
PEPPKYFSVMKQINKKGPALLKDLPVVSEITSMEQVRGLLDSEQIVDTRPSQEFAKGHVKGAINIPFNKAFSNWAGWIID
YNRPVYFITNPHEADKLLKALHSIGIENVAGFMDSGKIIASGMFSLESYTEVTSLEIANAVENGLVQVVDVRNLTEWQEG
HIPNAQHIMLGTLQGRLNEIKSDKPILVQCLSGARSAIGASILQANNDFKQVLKLDGGISQWQKAGLPIV
>Mature_470_residues
MLLRYFYDEKLAHASYLVGCQKTGDAIVIDPSRNIKPYLQFAKEEGLNIVAAAETHIHADFVSGAREIGVTHQAKLFLSD
EGDADWKYQYVDEIKHQLVKDGDRFSIGKLIFEVMHTPGHTPESISFLLTDRGSNADRPIGIFTGDFVFVSDVGRPDLLE
KAAGIKGTADCGARAMFKSLLKFKQLPDYLQVWPAHGAGSSCGKALGAIPSSTVGYEKLFNWSMTYTNEKDFVKALLEGQ
PEPPKYFSVMKQINKKGPALLKDLPVVSEITSMEQVRGLLDSEQIVDTRPSQEFAKGHVKGAINIPFNKAFSNWAGWIID
YNRPVYFITNPHEADKLLKALHSIGIENVAGFMDSGKIIASGMFSLESYTEVTSLEIANAVENGLVQVVDVRNLTEWQEG
HIPNAQHIMLGTLQGRLNEIKSDKPILVQCLSGARSAIGASILQANNDFKQVLKLDGGISQWQKAGLPIV

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787158, Length=219, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538952, Length=271, Percent_Identity=27.3062730627306, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI221330176, Length=241, Percent_Identity=26.1410788381743, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese [H]

EC number: 3.1.2.6

Molecular weight: Translated: 51769; Mature: 51769

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRYFYDEKLAHASYLVGCQKTGDAIVIDPSRNIKPYLQFAKEEGLNIVAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHH
FVSGAREIGVTHQAKLFLSDEGDADWKYQYVDEIKHQLVKDGDRFSIGKLIFEVMHTPGH
HHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
TPESISFLLTDRGSNADRPIGIFTGDFVFVSDVGRPDLLEKAAGIKGTADCGARAMFKSL
CCCCEEEEEEECCCCCCCCEEEEECCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHH
LKFKQLPDYLQVWPAHGAGSSCGKALGAIPSSTVGYEKLFNWSMTYTNEKDFVKALLEGQ
HHHHHCCHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHCCCEEECCHHHHHHHHHCCC
PEPPKYFSVMKQINKKGPALLKDLPVVSEITSMEQVRGLLDSEQIVDTRPSQEFAKGHVK
CCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHCCCCC
GAINIPFNKAFSNWAGWIIDYNRPVYFITNPHEADKLLKALHSIGIENVAGFMDSGKIIA
EEEECCHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCEEE
SGMFSLESYTEVTSLEIANAVENGLVQVVDVRNLTEWQEGHIPNAQHIMLGTLQGRLNEI
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCEEEEEHHHHHHHHH
KSDKPILVQCLSGARSAIGASILQANNDFKQVLKLDGGISQWQKAGLPIV
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHCCCCCC
>Mature Secondary Structure
MLLRYFYDEKLAHASYLVGCQKTGDAIVIDPSRNIKPYLQFAKEEGLNIVAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHH
FVSGAREIGVTHQAKLFLSDEGDADWKYQYVDEIKHQLVKDGDRFSIGKLIFEVMHTPGH
HHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
TPESISFLLTDRGSNADRPIGIFTGDFVFVSDVGRPDLLEKAAGIKGTADCGARAMFKSL
CCCCEEEEEEECCCCCCCCEEEEECCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHH
LKFKQLPDYLQVWPAHGAGSSCGKALGAIPSSTVGYEKLFNWSMTYTNEKDFVKALLEGQ
HHHHHCCHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHCCCEEECCHHHHHHHHHCCC
PEPPKYFSVMKQINKKGPALLKDLPVVSEITSMEQVRGLLDSEQIVDTRPSQEFAKGHVK
CCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHCCCCC
GAINIPFNKAFSNWAGWIIDYNRPVYFITNPHEADKLLKALHSIGIENVAGFMDSGKIIA
EEEECCHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCEEE
SGMFSLESYTEVTSLEIANAVENGLVQVVDVRNLTEWQEGHIPNAQHIMLGTLQGRLNEI
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCEEEEEHHHHHHHHH
KSDKPILVQCLSGARSAIGASILQANNDFKQVLKLDGGISQWQKAGLPIV
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]