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The map label for this gene is odhB [H]

Identifier: 154686344

GI number: 154686344

Start: 2030347

End: 2031594

Strand: Reverse

Name: odhB [H]

Synonym: RBAM_019120

Alternate gene names: 154686344

Gene position: 2031594-2030347 (Counterclockwise)

Preceding gene: 154686345

Following gene: 154686343

Centisome position: 51.85

GC content: 47.84

Gene sequence:

>1248_bases
ATGGCGGAAATTAAGGTACCTGAATTAGCAGAATCAATCTCAGAAGGAACGATAGCCCAATGGTTAAAGCAGCCCGGTGA
TTATGTAGAACAGGGTGAGTACTTGCTTGAACTGGAGACGGACAAAGTAAATGTTGAATTGACAGCAGAAGAATCGGGTG
TTCTAAAAGAGGTATTGAAAGATTCGGGTGATACCGTCCAAGTAGGAGAAATTATCGGTACCATTACAGAAGGCGCTGGC
GAAAGTTCTGCACCCGCCCCTTCAGAAAGTGCTCCAGCCAATGAACAAACGAAAGAGGAAGCAAAAGCTGAACCTGCTGC
ACAAGAAGTCAGCCAGGAAGCACAATCAGAAGCGAAATCCAGAACGGTAGCGTCTCCGGCTGCACGCAAGCTGGCAAGGG
AAAAAGGAATTGATCTATCACAAATTCCGACGGGGGACCCGCTCGGCCGCGTGCGCAAACAAGATGTGGAAGCTTATGAA
AAACCGGCTTCAAAACCTGCGGCACAGCCGAAACAGCAGGCTCAAAAAACACAGCAAAGCTTTGACAAACCTGTCGAAGT
GCAAAAAATGTCCCGCCGCAGACAGACGATCGCGAAACGATTGGTTGAAGTGCAGCAGACTTCTGCGATGCTGACTACAT
TTAACGAAGTTGACATGACGGCCGTTATGAATCTAAGAAAACGCCGCAAAGACCAATTCCTTGAGCAAAACGATGTAAAA
CTCGGTTTCATGTCGTTCTTTACAAAAGCCGTCGTGGCTGCATTGAAAAAATTCCCGCTCCTTAACGCTGAAATTCAAGG
CGATGAGCTGATCGTGAAAAAATTCTATGACATCGGTATTGCTGTCGCGGCTGAAGAAGGGCTTGTCGTCCCAGTCGTGC
GTGACGCTGACCGCCTGACGTTTGCCGGCATTGAAAGAGAGATCGGCGAACTTGCGAAGAAAGCGCGAAACAATAAATTA
ACATTAGGTGAGCTGCAAGGAGGCTCCTTCACGATCACAAACGGAGGTACATTCGGATCGCTTATGTCAACACCGATCCT
AAACAGCCCTCAGGTTGGGATCTTAGGTATGCACAAAATTCAGCTGCGCCCAGTAGCGATTGATGCGGAGCGCTCTGAAA
ACCGTCCGATGATGTACATCGCTTTATCATACGATCACAGAATTGTAGATGGTAAAGAAGCGGTCGGATTCCTTGTCACC
ATTAAAAACCTGCTTGAAGATCCGGAACAGCTGTTGCTGGAAGGATAA

Upstream 100 bases:

>100_bases
ATCCAGTCCCGCAGAAGGAGATCCGACAGTCCACAAAAAAGAACAGGAACGCATTGTATCTGATAGCTTGACTCGCAAAA
ACTAAGGGGGAAATGAAAAA

Downstream 100 bases:

>100_bases
TTTGAGACAGACAAAAAGTACACTGCCCTTACAAGCAGTGTACTTTTTTTATTGAGAGAAGGGGAGAATTTATATGAAAA
GCTGTCATCCGCTTGAGATC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MAEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLKEVLKDSGDTVQVGEIIGTITEGAG
ESSAPAPSESAPANEQTKEEAKAEPAAQEVSQEAQSEAKSRTVASPAARKLAREKGIDLSQIPTGDPLGRVRKQDVEAYE
KPASKPAAQPKQQAQKTQQSFDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFLEQNDVK
LGFMSFFTKAVVAALKKFPLLNAEIQGDELIVKKFYDIGIAVAAEEGLVVPVVRDADRLTFAGIEREIGELAKKARNNKL
TLGELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMHKIQLRPVAIDAERSENRPMMYIALSYDHRIVDGKEAVGFLVT
IKNLLEDPEQLLLEG

Sequences:

>Translated_415_residues
MAEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLKEVLKDSGDTVQVGEIIGTITEGAG
ESSAPAPSESAPANEQTKEEAKAEPAAQEVSQEAQSEAKSRTVASPAARKLAREKGIDLSQIPTGDPLGRVRKQDVEAYE
KPASKPAAQPKQQAQKTQQSFDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFLEQNDVK
LGFMSFFTKAVVAALKKFPLLNAEIQGDELIVKKFYDIGIAVAAEEGLVVPVVRDADRLTFAGIEREIGELAKKARNNKL
TLGELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMHKIQLRPVAIDAERSENRPMMYIALSYDHRIVDGKEAVGFLVT
IKNLLEDPEQLLLEG
>Mature_414_residues
AEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLKEVLKDSGDTVQVGEIIGTITEGAGE
SSAPAPSESAPANEQTKEEAKAEPAAQEVSQEAQSEAKSRTVASPAARKLAREKGIDLSQIPTGDPLGRVRKQDVEAYEK
PASKPAAQPKQQAQKTQQSFDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFLEQNDVKL
GFMSFFTKAVVAALKKFPLLNAEIQGDELIVKKFYDIGIAVAAEEGLVVPVVRDADRLTFAGIEREIGELAKKARNNKLT
LGELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMHKIQLRPVAIDAERSENRPMMYIALSYDHRIVDGKEAVGFLVTI
KNLLEDPEQLLLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=54.1484716157205, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=29.6052631578947, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=29.3859649122807, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=30.5747126436782, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=26.6203703703704, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=35.1190476190476, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786946, Length=413, Percent_Identity=46.2469733656174, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1786305, Length=427, Percent_Identity=32.7868852459016, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI25146366, Length=412, Percent_Identity=39.5631067961165, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=31.4220183486239, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=435, Percent_Identity=26.2068965517241, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=329, Percent_Identity=29.1793313069909, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=425, Percent_Identity=40.4705882352941, Blast_Score=305, Evalue=9e-84,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=28, Blast_Score=159, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24645909, Length=220, Percent_Identity=52.2727272727273, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=26.3403263403263, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI20129315, Length=443, Percent_Identity=26.6365688487585, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=431, Percent_Identity=26.9141531322506, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45417; Mature: 45286

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLKEVLK
CCCCCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
DSGDTVQVGEIIGTITEGAGESSAPAPSESAPANEQTKEEAKAEPAAQEVSQEAQSEAKS
CCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
RTVASPAARKLAREKGIDLSQIPTGDPLGRVRKQDVEAYEKPASKPAAQPKQQAQKTQQS
HHHCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFLEQNDVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LGFMSFFTKAVVAALKKFPLLNAEIQGDELIVKKFYDIGIAVAAEEGLVVPVVRDADRLT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEEECCCEEEEEECCCCHHH
FAGIEREIGELAKKARNNKLTLGELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMHKI
HHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEE
QLRPVAIDAERSENRPMMYIALSYDHRIVDGKEAVGFLVTIKNLLEDPEQLLLEG
EEEEEEEECCCCCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
AEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLKEVLK
CCCCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
DSGDTVQVGEIIGTITEGAGESSAPAPSESAPANEQTKEEAKAEPAAQEVSQEAQSEAKS
CCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
RTVASPAARKLAREKGIDLSQIPTGDPLGRVRKQDVEAYEKPASKPAAQPKQQAQKTQQS
HHHCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFLEQNDVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LGFMSFFTKAVVAALKKFPLLNAEIQGDELIVKKFYDIGIAVAAEEGLVVPVVRDADRLT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEEECCCEEEEEECCCCHHH
FAGIEREIGELAKKARNNKLTLGELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMHKI
HHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEE
QLRPVAIDAERSENRPMMYIALSYDHRIVDGKEAVGFLVTIKNLLEDPEQLLLEG
EEEEEEEECCCCCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377 [H]