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The map label for this gene is proJ [H]

Identifier: 154686296

GI number: 154686296

Start: 1989035

End: 1990153

Strand: Reverse

Name: proJ [H]

Synonym: RBAM_018640

Alternate gene names: 154686296

Gene position: 1990153-1989035 (Counterclockwise)

Preceding gene: 154686297

Following gene: 154686294

Centisome position: 50.79

GC content: 48.7

Gene sequence:

>1119_bases
GTGACGTCTCGGACGAAGATCAAAAGAGTCGCCGTGAAAATCGGCAGCAGCTCACTGACGAGCCTGCACGGGGAAATCAG
CAGAAGAAAGCTGGAAGCATTGGTGGATCAAATCGCACAGCTGAAAGACAGCGGCTATGAAGTGATTTTAGTATCTTCCG
GCGCAGTGGCGGCAGGTTATCGGAAGCTCGGATTTATTCAGAAACCGGAAAGCCTCCCTGAAAAACAGGCTTCTGCTTCC
ATCGGGCAGGGATTATTAATGGAGGCTTATTCAAAGCTGTTTTTAGCGCACGGTTATGTCGCATCACAAATTTTAATTAC
AAGAAGCGACTTTTCCGATGAATACCGCTACCATAATGTGCGCAATACGATGAACGTCCTTTTGGAACGGGGCATTATTC
CGATTATTAATGAAAATGACACGGTCACAGTCAACCGGCTGAGATTCGGAGATAACGATACACTCGCAGCGAAGGTGGCA
GGTTTAATTGATGCCGACATGCTTGTGATTTTATCAGACATTGACGGTTTATATGATGATAATCCGCGCAGCAACCCGAA
AGCGAAAAGAATCAGACATGTAAGTGACATCACTCCGGAAATAGAAGCAAGCGCGGGAGATGCCGGGAGTGAAATGGGAA
CCGGCGGAATGCGTTCGAAACTGGATGCTTTTAAAATTGTGATGGCCTCCGGTATTAAAGGCTTTCTCGGGCAGGCTAAC
ATTCCGGACATTCTGCTTCAAGCGGTAGATGAGGCGGCTGAAGGAACTTATTTTGAAGCGGAAGGAACCCTGCCGTTAAA
CCACAAAGAACAATGGCTTGCATTTAATTCCGGCCCCGAAGGCGAAATTGTGATGGAAAAAGAAAGCAGCCGCGATGTCG
CGAACGGAAATATGGAGCTGTCACCGCAGGACATCGAGCAGATTAAGGGCAGATTCAGCAGCGGAGCGGTTGTCAGGCTG
CTTGATCAGCAGGGAGAAGAGCTCGGACTCGGCATCGTCAACTATTCCTCAGCCCAGCTGAAGACTTTTAAAGAAACGCC
GCCCAAACAGACCGATCAATCAGTCATTGATCCGGAAGCGTTTGTCTGCCACGTTGAACTATCCATTCCGATCGGCTGA

Upstream 100 bases:

>100_bases
TGCCAAAGCCATTAAACACGCGACCGAACGTTCTATTGAGATCAGCAAAACAATTGAAAAAACGGCAGCACTATAATCGT
TAAAAAACAGGAGTGATACA

Downstream 100 bases:

>100_bases
GAAAGATCTATAAAAATATGAAAAAGCCCGAGGCTTCGTTTTGAAGCTCGGGTTTGTTTTTTATTGATATTGTTCAAGTT
TGGAGAAAAATTGAATGACA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase 2; GK 2 [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTSRTKIKRVAVKIGSSSLTSLHGEISRRKLEALVDQIAQLKDSGYEVILVSSGAVAAGYRKLGFIQKPESLPEKQASAS
IGQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYHNVRNTMNVLLERGIIPIINENDTVTVNRLRFGDNDTLAAKVA
GLIDADMLVILSDIDGLYDDNPRSNPKAKRIRHVSDITPEIEASAGDAGSEMGTGGMRSKLDAFKIVMASGIKGFLGQAN
IPDILLQAVDEAAEGTYFEAEGTLPLNHKEQWLAFNSGPEGEIVMEKESSRDVANGNMELSPQDIEQIKGRFSSGAVVRL
LDQQGEELGLGIVNYSSAQLKTFKETPPKQTDQSVIDPEAFVCHVELSIPIG

Sequences:

>Translated_372_residues
MTSRTKIKRVAVKIGSSSLTSLHGEISRRKLEALVDQIAQLKDSGYEVILVSSGAVAAGYRKLGFIQKPESLPEKQASAS
IGQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYHNVRNTMNVLLERGIIPIINENDTVTVNRLRFGDNDTLAAKVA
GLIDADMLVILSDIDGLYDDNPRSNPKAKRIRHVSDITPEIEASAGDAGSEMGTGGMRSKLDAFKIVMASGIKGFLGQAN
IPDILLQAVDEAAEGTYFEAEGTLPLNHKEQWLAFNSGPEGEIVMEKESSRDVANGNMELSPQDIEQIKGRFSSGAVVRL
LDQQGEELGLGIVNYSSAQLKTFKETPPKQTDQSVIDPEAFVCHVELSIPIG
>Mature_371_residues
TSRTKIKRVAVKIGSSSLTSLHGEISRRKLEALVDQIAQLKDSGYEVILVSSGAVAAGYRKLGFIQKPESLPEKQASASI
GQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYHNVRNTMNVLLERGIIPIINENDTVTVNRLRFGDNDTLAAKVAG
LIDADMLVILSDIDGLYDDNPRSNPKAKRIRHVSDITPEIEASAGDAGSEMGTGGMRSKLDAFKIVMASGIKGFLGQANI
PDILLQAVDEAAEGTYFEAEGTLPLNHKEQWLAFNSGPEGEIVMEKESSRDVANGNMELSPQDIEQIKGRFSSGAVVRLL
DQQGEELGLGIVNYSSAQLKTFKETPPKQTDQSVIDPEAFVCHVELSIPIG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=364, Percent_Identity=29.6703296703297, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI62912457, Length=362, Percent_Identity=29.2817679558011, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1786437, Length=343, Percent_Identity=35.5685131195335, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17569967, Length=269, Percent_Identity=32.3420074349442, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=269, Percent_Identity=32.3420074349442, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=398, Percent_Identity=32.6633165829146, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6320506, Length=243, Percent_Identity=40.7407407407407, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI21357643, Length=268, Percent_Identity=32.4626865671642, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40439; Mature: 40308

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRTKIKRVAVKIGSSSLTSLHGEISRRKLEALVDQIAQLKDSGYEVILVSSGAVAAGY
CCCHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
RKLGFIQKPESLPEKQASASIGQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYHNV
HHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHEEEEEECCCCCHHHHHHH
RNTMNVLLERGIIPIINENDTVTVNRLRFGDNDTLAAKVAGLIDADMLVILSDIDGLYDD
HHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCHHEEEEHHCCCCCC
NPRSNPKAKRIRHVSDITPEIEASAGDAGSEMGTGGMRSKLDAFKIVMASGIKGFLGQAN
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCC
IPDILLQAVDEAAEGTYFEAEGTLPLNHKEQWLAFNSGPEGEIVMEKESSRDVANGNMEL
CHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCEE
SPQDIEQIKGRFSSGAVVRLLDQQGEELGLGIVNYSSAQLKTFKETPPKQTDQSVIDPEA
CHHHHHHHHCCCCCCCEEEEEHHCCCHHCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCE
FVCHVELSIPIG
EEEEEEEEECCC
>Mature Secondary Structure 
TSRTKIKRVAVKIGSSSLTSLHGEISRRKLEALVDQIAQLKDSGYEVILVSSGAVAAGY
CCHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
RKLGFIQKPESLPEKQASASIGQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYHNV
HHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHEEEEEECCCCCHHHHHHH
RNTMNVLLERGIIPIINENDTVTVNRLRFGDNDTLAAKVAGLIDADMLVILSDIDGLYDD
HHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCHHEEEEHHCCCCCC
NPRSNPKAKRIRHVSDITPEIEASAGDAGSEMGTGGMRSKLDAFKIVMASGIKGFLGQAN
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCC
IPDILLQAVDEAAEGTYFEAEGTLPLNHKEQWLAFNSGPEGEIVMEKESSRDVANGNMEL
CHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCEE
SPQDIEQIKGRFSSGAVVRLLDQQGEELGLGIVNYSSAQLKTFKETPPKQTDQSVIDPEA
CHHHHHHHHCCCCCCCEEEEEHHCCCHHCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCE
FVCHVELSIPIG
EEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]