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The map label for this gene is gltD [H]

Identifier: 154686293

GI number: 154686293

Start: 1981869

End: 1983350

Strand: Reverse

Name: gltD [H]

Synonym: RBAM_018610

Alternate gene names: 154686293

Gene position: 1983350-1981869 (Counterclockwise)

Preceding gene: 154686294

Following gene: 154686292

Centisome position: 50.61

GC content: 49.6

Gene sequence:

>1482_bases
ATGGGGAAACCAACTGGATTCATGGAAGAAAAACGAGAAAAACCGGCTGAGCGGGACCCTCTCACTCGCTTAAAGGATTG
GAAAGAGTATTCGGCTCCGTTTTCAGATGAAGCGTCAAAGCGGCAGGGGGCGCGGTGTATGGATTGCGGCACACCGTTTT
GCCAAATCGGCGCTGATATCAACGGTTTTACCTCCGGATGCCCGATTTATAATCTGATACCGGAGTGGAATGACCTCGTC
TATCGGGGGCGCTGGAAAGAAGCCTTGGAGCGCCTTCTGAAAACGAATAACTTTCCTGAATTCACAGGGCGGGTCTGTCC
CGCTCCTTGTGAAGGATCTTGTACGCTGGCTATTTCAGATCCGGCTGTTTCAATTAAAAACATCGAGCGGACGATCATTG
ACAAAGGTTTTGAGAACGGATGGATTCAGCCGAGAATTCCAGCGAAACGCACAGGTAAAAAAGTCGCTGTCGTCGGTTCA
GGCCCGGCAGGTCTGGCAAGCGCTGATCAGCTGAACCAAGCCGGTCATTCCGTAACGGTCTATGAGCGTGCTGACAGAGC
CGGCGGTCTGCTGATGTACGGCATTCCGAGCATGAAGCTTGAGAAAAGTGTCGTCGAACGCCGTATTAAACTTTTGACGC
AGGAAGGTATAGATTTTGTAACAAATACCGAAATCGGCGTTGACATAACGGCAGATGAGCTGAAAGATCAATTTGATGCC
GTCATTTTGTGCACAGGCGCCCAAAAGCAGCGCAATCTGCTGATTGAAGGCCGGGAAGCAAAAGGCGTCCATTATGCGAT
GGACTATTTAACACTGGCGACGAAAAGCTATTTAGATTCCAACTTCAAAGACAAACAATATATAGACGCTAAAGGGAAAG
ACGTCATCGTAATCGGCGGAGGAGACACAGGAGCTGACTGCGTGGCAACCGCGCTGCGCCAAAAAGCGCGCAGCGTGCAC
CAGTTCGGGAAACATCCTAAACTTCCGCAAACGCGCACGAACGACAATATGTGGCCTGAACAGCCGCACGTCTTTACCCT
TGAATACGCCTATGAAGAAGCGCAGGCAAAGTTCGGCAAAGACCCGAGAGAATATTCAATTCAGACGACAAAAATGGTGG
CCGATAAGCAGGGCCGCTTACAAGAGATTCACACCATTCAAATGGAAAAAGTCAGAAACGAATTCGGAAAGTATGAATTT
CATGAACTGCCCGGAACAGAAAAAGTCTGGCCTGCTCAGCTGGTGTTCATCGCCATCGGTTTTGAAGGGACGGAACAGCC
ATTATTGAAACAGTTCGGTGTCGATTCGGCAAATCATAAAATTCAGGCGGCATATGGGGATTATAAAACGAATGTTGACG
GTGTTTTCGCCGCAGGGGATGCGAGAAGGGGCCAAAGCCTGATTGTATGGGCGATTAATGAAGGCCGGGAAGCAGCGCGC
GAAGTCGACAGTTATTTAATGGGGAGCACTGTTTTACCGTAA

Upstream 100 bases:

>100_bases
GACGCAATTATGTATGCTTTTGAAGCCAATACAAAGGTTCACCAAACGTCTGCCAAAGACCATAAACAAGCGGTAGTACA
GTAAGAAAGGGGAGAGGAAC

Downstream 100 bases:

>100_bases
ATAAAGGGGTTTATTTATAAAACCGGCTGGGATCAGCCGGTTTTTCGTATTGAAATGAAACCTCCTTCTTCCTTTATTGG
TCATATAAGGGTAACGTGGA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MGKPTGFMEEKREKPAERDPLTRLKDWKEYSAPFSDEASKRQGARCMDCGTPFCQIGADINGFTSGCPIYNLIPEWNDLV
YRGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISDPAVSIKNIERTIIDKGFENGWIQPRIPAKRTGKKVAVVGS
GPAGLASADQLNQAGHSVTVYERADRAGGLLMYGIPSMKLEKSVVERRIKLLTQEGIDFVTNTEIGVDITADELKDQFDA
VILCTGAQKQRNLLIEGREAKGVHYAMDYLTLATKSYLDSNFKDKQYIDAKGKDVIVIGGGDTGADCVATALRQKARSVH
QFGKHPKLPQTRTNDNMWPEQPHVFTLEYAYEEAQAKFGKDPREYSIQTTKMVADKQGRLQEIHTIQMEKVRNEFGKYEF
HELPGTEKVWPAQLVFIAIGFEGTEQPLLKQFGVDSANHKIQAAYGDYKTNVDGVFAAGDARRGQSLIVWAINEGREAAR
EVDSYLMGSTVLP

Sequences:

>Translated_493_residues
MGKPTGFMEEKREKPAERDPLTRLKDWKEYSAPFSDEASKRQGARCMDCGTPFCQIGADINGFTSGCPIYNLIPEWNDLV
YRGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISDPAVSIKNIERTIIDKGFENGWIQPRIPAKRTGKKVAVVGS
GPAGLASADQLNQAGHSVTVYERADRAGGLLMYGIPSMKLEKSVVERRIKLLTQEGIDFVTNTEIGVDITADELKDQFDA
VILCTGAQKQRNLLIEGREAKGVHYAMDYLTLATKSYLDSNFKDKQYIDAKGKDVIVIGGGDTGADCVATALRQKARSVH
QFGKHPKLPQTRTNDNMWPEQPHVFTLEYAYEEAQAKFGKDPREYSIQTTKMVADKQGRLQEIHTIQMEKVRNEFGKYEF
HELPGTEKVWPAQLVFIAIGFEGTEQPLLKQFGVDSANHKIQAAYGDYKTNVDGVFAAGDARRGQSLIVWAINEGREAAR
EVDSYLMGSTVLP
>Mature_492_residues
GKPTGFMEEKREKPAERDPLTRLKDWKEYSAPFSDEASKRQGARCMDCGTPFCQIGADINGFTSGCPIYNLIPEWNDLVY
RGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISDPAVSIKNIERTIIDKGFENGWIQPRIPAKRTGKKVAVVGSG
PAGLASADQLNQAGHSVTVYERADRAGGLLMYGIPSMKLEKSVVERRIKLLTQEGIDFVTNTEIGVDITADELKDQFDAV
ILCTGAQKQRNLLIEGREAKGVHYAMDYLTLATKSYLDSNFKDKQYIDAKGKDVIVIGGGDTGADCVATALRQKARSVHQ
FGKHPKLPQTRTNDNMWPEQPHVFTLEYAYEEAQAKFGKDPREYSIQTTKMVADKQGRLQEIHTIQMEKVRNEFGKYEFH
ELPGTEKVWPAQLVFIAIGFEGTEQPLLKQFGVDSANHKIQAAYGDYKTNVDGVFAAGDARRGQSLIVWAINEGREAARE
VDSYLMGSTVLP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=477, Percent_Identity=23.4800838574423, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1789606, Length=488, Percent_Identity=36.2704918032787, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788811, Length=498, Percent_Identity=34.3373493975904, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI87082180, Length=424, Percent_Identity=34.6698113207547, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1788468, Length=465, Percent_Identity=25.1612903225806, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789243, Length=445, Percent_Identity=27.8651685393258, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17570289, Length=498, Percent_Identity=46.5863453815261, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71984108, Length=482, Percent_Identity=24.6887966804979, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17543860, Length=164, Percent_Identity=31.0975609756098, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=496, Percent_Identity=49.3951612903226, Blast_Score=498, Evalue=1e-142,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=52.2448979591837, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=52.2448979591837, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=52.2448979591837, Blast_Score=526, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=52.2448979591837, Blast_Score=526, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24640763, Length=299, Percent_Identity=27.7591973244147, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI18858217, Length=299, Percent_Identity=27.7591973244147, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54889; Mature: 54758

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPTGFMEEKREKPAERDPLTRLKDWKEYSAPFSDEASKRQGARCMDCGTPFCQIGADI
CCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCCHHHHCCCC
NGFTSGCPIYNLIPEWNDLVYRGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISD
CCCCCCCCCEECCCCHHHHEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVSIKNIERTIIDKGFENGWIQPRIPAKRTGKKVAVVGSGPAGLASADQLNQAGHSVTV
CCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCCHHHHHHCCCEEEE
YERADRAGGLLMYGIPSMKLEKSVVERRIKLLTQEGIDFVTNTEIGVDITADELKDQFDA
EECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHCCCE
VILCTGAQKQRNLLIEGREAKGVHYAMDYLTLATKSYLDSNFKDKQYIDAKGKDVIVIGG
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEEC
GDTGADCVATALRQKARSVHQFGKHPKLPQTRTNDNMWPEQPHVFTLEYAYEEAQAKFGK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCC
DPREYSIQTTKMVADKQGRLQEIHTIQMEKVRNEFGKYEFHELPGTEKVWPAQLVFIAIG
CCHHHCEEHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEEC
FEGTEQPLLKQFGVDSANHKIQAAYGDYKTNVDGVFAAGDARRGQSLIVWAINEGREAAR
CCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCEEEEEEECCCHHHHH
EVDSYLMGSTVLP
HHHHHHHCCCCCC
>Mature Secondary Structure 
GKPTGFMEEKREKPAERDPLTRLKDWKEYSAPFSDEASKRQGARCMDCGTPFCQIGADI
CCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCCHHHHCCCC
NGFTSGCPIYNLIPEWNDLVYRGRWKEALERLLKTNNFPEFTGRVCPAPCEGSCTLAISD
CCCCCCCCCEECCCCHHHHEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVSIKNIERTIIDKGFENGWIQPRIPAKRTGKKVAVVGSGPAGLASADQLNQAGHSVTV
CCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCCHHHHHHCCCEEEE
YERADRAGGLLMYGIPSMKLEKSVVERRIKLLTQEGIDFVTNTEIGVDITADELKDQFDA
EECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHCCCE
VILCTGAQKQRNLLIEGREAKGVHYAMDYLTLATKSYLDSNFKDKQYIDAKGKDVIVIGG
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEEC
GDTGADCVATALRQKARSVHQFGKHPKLPQTRTNDNMWPEQPHVFTLEYAYEEAQAKFGK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCC
DPREYSIQTTKMVADKQGRLQEIHTIQMEKVRNEFGKYEFHELPGTEKVWPAQLVFIAIG
CCHHHCEEHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEEC
FEGTEQPLLKQFGVDSANHKIQAAYGDYKTNVDGVFAAGDARRGQSLIVWAINEGREAAR
CCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCEEEEEEECCCHHHHH
EVDSYLMGSTVLP
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]