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The map label for this gene is yofA

Identifier: 154686290

GI number: 154686290

Start: 1979581

End: 1980447

Strand: Reverse

Name: yofA

Synonym: RBAM_018580

Alternate gene names: 154686290

Gene position: 1980447-1979581 (Counterclockwise)

Preceding gene: 154686292

Following gene: 154686289

Centisome position: 50.54

GC content: 46.71

Gene sequence:

>867_bases
ATGGAAAGCGGAGACTTAAAGATTTTTCAGGCTGTCGCCCGCAAAGGAAGTATTTCAAAGGCGGCAGAAAGCCTGCATTA
TGTTCAATCAAATGTGACAAACAGGATTCAGCAGCTGGAACGGCAATTGCAAACACAGCTTTTTTACCGCACAAATCGGG
GGATGACGCTGACGCCTGCAGGTGAAAACTTATTGAAAGACGCAGACAGAATCCTTCAATTGCTGCATGAGGCGCAAAAA
ACGGCGCAAGAGGCAGGCGATCCGAACGGCCCGCTTCGAATCGGTTCTCTTGAAACGGCGGCAGCCGTCCATCTCCCTCA
ATTTTTCACAGAGTATTGCAGCCGCTACCCGAAAGTACAGCTGTCTCTGTCAACCGGTGATACACATACCTTAGTGCAGC
ACATCTTACACTATGAGCTGGACGGCGCTTTTGTTTATGGTCCGGTCGAATACGAAGATCTTGAGCAAGTCGCTGTATTT
CAAGAAGAATTGGTCTTGGCTTCAAATAAGCGGGAAGGCAGTTTGCAAGACTTGTTGCGAGAGCCGATTCTGTATTTTGC
CGCGGGCTGTTCGCACCGCCGCAAAATAAAAAACATACTGAAAGAAGAAGCAGTACCCGCACATAAAATCATTGAATTCG
GTACGTTGGAAGCCATTATTGGGGCGGTGCAGGCGGGGATGGGGGTTTCCTTTCTGCCTGCATCAGCCGTCCGTTATTTT
CAAAACAGGAAAAATCTTTTTCTTCATGAGCTTCCTGAACAGTTTCGGGATATGAATATATCATTCATCTACCAGAGTGA
TTTATTTTTGACATCGGCGTTTGAAGAACTGCTGAATGGATTAAAAGCATTACCGAACGGAAGATGA

Upstream 100 bases:

>100_bases
GATTGATGAGAAAGATTTTACAGGCTGTATCAGCTGCAGTAAATAAATGAAAATTGATAGCCGGCTATCAATAAAAATCA
TAGCAAGAAGGTGCCCTTAC

Downstream 100 bases:

>100_bases
GAAAACAGGACAGCTGTGTACGCCAATTTTTTCGTTCCGGTTTTCTCAAAAGAAAGCGGGTATCCGGGCTGAGCTTGATC
AGCGCGATGAAAAACTCGGC

Product: YofA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MESGDLKIFQAVARKGSISKAAESLHYVQSNVTNRIQQLERQLQTQLFYRTNRGMTLTPAGENLLKDADRILQLLHEAQK
TAQEAGDPNGPLRIGSLETAAAVHLPQFFTEYCSRYPKVQLSLSTGDTHTLVQHILHYELDGAFVYGPVEYEDLEQVAVF
QEELVLASNKREGSLQDLLREPILYFAAGCSHRRKIKNILKEEAVPAHKIIEFGTLEAIIGAVQAGMGVSFLPASAVRYF
QNRKNLFLHELPEQFRDMNISFIYQSDLFLTSAFEELLNGLKALPNGR

Sequences:

>Translated_288_residues
MESGDLKIFQAVARKGSISKAAESLHYVQSNVTNRIQQLERQLQTQLFYRTNRGMTLTPAGENLLKDADRILQLLHEAQK
TAQEAGDPNGPLRIGSLETAAAVHLPQFFTEYCSRYPKVQLSLSTGDTHTLVQHILHYELDGAFVYGPVEYEDLEQVAVF
QEELVLASNKREGSLQDLLREPILYFAAGCSHRRKIKNILKEEAVPAHKIIEFGTLEAIIGAVQAGMGVSFLPASAVRYF
QNRKNLFLHELPEQFRDMNISFIYQSDLFLTSAFEELLNGLKALPNGR
>Mature_288_residues
MESGDLKIFQAVARKGSISKAAESLHYVQSNVTNRIQQLERQLQTQLFYRTNRGMTLTPAGENLLKDADRILQLLHEAQK
TAQEAGDPNGPLRIGSLETAAAVHLPQFFTEYCSRYPKVQLSLSTGDTHTLVQHILHYELDGAFVYGPVEYEDLEQVAVF
QEELVLASNKREGSLQDLLREPILYFAAGCSHRRKIKNILKEEAVPAHKIIEFGTLEAIIGAVQAGMGVSFLPASAVRYF
QNRKNLFLHELPEQFRDMNISFIYQSDLFLTSAFEELLNGLKALPNGR

Specific function: Regulates expression of the cell division protein ftsW, and is essential for cell viability during stationary phase

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787806, Length=269, Percent_Identity=33.457249070632, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI87082132, Length=247, Percent_Identity=29.1497975708502, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=26.9360269360269, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=243, Percent_Identity=26.7489711934156, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=29.2490118577075, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788481, Length=233, Percent_Identity=28.755364806867, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=29.4820717131474, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693105, Length=247, Percent_Identity=26.3157894736842, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=26.5873015873016, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=27.6859504132231, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787601, Length=151, Percent_Identity=31.1258278145695, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786713, Length=259, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787530, Length=240, Percent_Identity=22.5, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787128, Length=163, Percent_Identity=29.4478527607362, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788887, Length=167, Percent_Identity=30.5389221556886, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=159, Percent_Identity=30.8176100628931, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790262, Length=252, Percent_Identity=26.984126984127, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87081904, Length=295, Percent_Identity=23.0508474576271, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=190, Percent_Identity=28.4210526315789, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YOFA_BACA2 (A7Z5E4)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421451.1
- ProteinModelPortal:   A7Z5E4
- STRING:   A7Z5E4
- EnsemblBacteria:   EBBACT00000007621
- GeneID:   5460938
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_018580
- eggNOG:   COG0583
- GeneTree:   EBGT00070000031761
- HOGENOM:   HBG685425
- OMA:   EEMVIIA
- ProtClustDB:   CLSK887372
- BioCyc:   BAMY326423:RBAM_018580-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 32392; Mature: 32392

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESGDLKIFQAVARKGSISKAAESLHYVQSNVTNRIQQLERQLQTQLFYRTNRGMTLTPA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
GENLLKDADRILQLLHEAQKTAQEAGDPNGPLRIGSLETAAAVHLPQFFTEYCSRYPKVQ
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEE
LSLSTGDTHTLVQHILHYELDGAFVYGPVEYEDLEQVAVFQEELVLASNKREGSLQDLLR
EEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EPILYFAAGCSHRRKIKNILKEEAVPAHKIIEFGTLEAIIGAVQAGMGVSFLPASAVRYF
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QNRKNLFLHELPEQFRDMNISFIYQSDLFLTSAFEELLNGLKALPNGR
HHCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MESGDLKIFQAVARKGSISKAAESLHYVQSNVTNRIQQLERQLQTQLFYRTNRGMTLTPA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
GENLLKDADRILQLLHEAQKTAQEAGDPNGPLRIGSLETAAAVHLPQFFTEYCSRYPKVQ
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEE
LSLSTGDTHTLVQHILHYELDGAFVYGPVEYEDLEQVAVFQEELVLASNKREGSLQDLLR
EEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EPILYFAAGCSHRRKIKNILKEEAVPAHKIIEFGTLEAIIGAVQAGMGVSFLPASAVRYF
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QNRKNLFLHELPEQFRDMNISFIYQSDLFLTSAFEELLNGLKALPNGR
HHCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA