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The map label for this gene is def [H]

Identifier: 154685863

GI number: 154685863

Start: 1389644

End: 1390198

Strand: Reverse

Name: def [H]

Synonym: RBAM_014300

Alternate gene names: 154685863

Gene position: 1390198-1389644 (Counterclockwise)

Preceding gene: 154685865

Following gene: 154685861

Centisome position: 35.48

GC content: 48.47

Gene sequence:

>555_bases
TTGATTACTATGGAAAATATTGTACGCGACGGACATCCCGCGCTCAGAGAGATCGCTGAAGAGGTTCAGCTTCCCGCTTC
TGACGAAGAAAAACAGCATCTTGCTGATATGATTGAGTTTGTGAAAAACAGCCAAAATCCTGAATTAGCCGAGAAATACA
AGCTTCGTCCGGGCGTCGGTCTTGCCGCTCCCCAAACAGACGTAAAAAAACGGATGATCGCCGTCCATGCAGAAGATGCG
AAAGGCACTTTATACAGCTATGCTCTTTTTAACCCTAAGATCGTCAGCCACTCAGTGGAAAAAAGCTACTTGACGAGCGG
AGAAGGCTGCCTGTCGGTAGACGAACCCATTCCCGGCTATGTGCCGCGCTACGCCCGCATCCGCGTAAAGGCGGTGACGC
TTGAAGGAGAACAGATTGACATCCGCCTCAAAGGTTTTCCGGCCATCGTGTTTCAGCACGAAATCGATCATCTGAACGGC
GTGATGTTTTACGACCATATTGATAAAGAAAATCCGTTCAAAGAACCTGAAAACGCCATTGCCATTGACCGTTAA

Upstream 100 bases:

>100_bases
TGAACATCGGCCGGCCTGCTATGGCCGGCCAATTGTTTCGTTGCGCCGCGATAAAGAAATTCGCTATAATTTACATATTA
AGCTTTAGGAGTGAAGTTTC

Downstream 100 bases:

>100_bases
AAATAACCGGCACTTCCCAAAGTGCCGGTTTTCTTTATTGCAAAAGGCCCAGTTCTTTGACGGCCCAGCTGATTCCTTCC
TCATCCACCGGCTTTGTGAC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MITMENIVRDGHPALREIAEEVQLPASDEEKQHLADMIEFVKNSQNPELAEKYKLRPGVGLAAPQTDVKKRMIAVHAEDA
KGTLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEPIPGYVPRYARIRVKAVTLEGEQIDIRLKGFPAIVFQHEIDHLNG
VMFYDHIDKENPFKEPENAIAIDR

Sequences:

>Translated_184_residues
MITMENIVRDGHPALREIAEEVQLPASDEEKQHLADMIEFVKNSQNPELAEKYKLRPGVGLAAPQTDVKKRMIAVHAEDA
KGTLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEPIPGYVPRYARIRVKAVTLEGEQIDIRLKGFPAIVFQHEIDHLNG
VMFYDHIDKENPFKEPENAIAIDR
>Mature_184_residues
MITMENIVRDGHPALREIAEEVQLPASDEEKQHLADMIEFVKNSQNPELAEKYKLRPGVGLAAPQTDVKKRMIAVHAEDA
KGTLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEPIPGYVPRYARIRVKAVTLEGEQIDIRLKGFPAIVFQHEIDHLNG
VMFYDHIDKENPFKEPENAIAIDR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=118, Percent_Identity=33.0508474576271, Blast_Score=72, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645728, Length=177, Percent_Identity=30.5084745762712, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20753; Mature: 20753

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMENIVRDGHPALREIAEEVQLPASDEEKQHLADMIEFVKNSQNPELAEKYKLRPGVG
CCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
LAAPQTDVKKRMIAVHAEDAKGTLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEPIPGY
CCCCCHHHHHEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEECCCCCCCC
VPRYARIRVKAVTLEGEQIDIRLKGFPAIVFQHEIDHLNGVMFYDHIDKENPFKEPENAI
CCCEEEEEEEEEEECCCEEEEEECCCEEEEEEECHHHCCCEEEEECCCCCCCCCCCCCCE
AIDR
ECCC
>Mature Secondary Structure
MITMENIVRDGHPALREIAEEVQLPASDEEKQHLADMIEFVKNSQNPELAEKYKLRPGVG
CCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
LAAPQTDVKKRMIAVHAEDAKGTLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEPIPGY
CCCCCHHHHHEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEECCCCCCCC
VPRYARIRVKAVTLEGEQIDIRLKGFPAIVFQHEIDHLNGVMFYDHIDKENPFKEPENAI
CCCEEEEEEEEEEECCCEEEEEECCCEEEEEEECHHHCCCEEEEECCCCCCCCCCCCCCE
AIDR
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11429456 [H]