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The map label for this gene is clpE [H]

Identifier: 154685779

GI number: 154685779

Start: 1307704

End: 1309800

Strand: Reverse

Name: clpE [H]

Synonym: RBAM_013460

Alternate gene names: 154685779

Gene position: 1309800-1307704 (Counterclockwise)

Preceding gene: 154685786

Following gene: 154685778

Centisome position: 33.43

GC content: 48.35

Gene sequence:

>2097_bases
ATGCGTTGTCAACATTGTCAGAAAAACGAGGCGACGATTCGCCTGAACATGCAAGTCAATTCCATTCAAAAACAATTCGT
TTTATGTGAAACATGTTATACAGAATTAACCCGAAAACCGGCTATGAGCATGGGGCCGCAATCATTCGGATTCCCGTTTG
AGCAAGGTTTCGCTCCCAATCAAAATAAACAGCAGGCCGGCCCTGAAAAAGGCCTGCTCGACGAGCTCGCTCAAAACGTC
ACAAACGGCGCAAAAGCAGGTCTGATTGACCCTGTGATCGGCCGCGATCATGAAGTGGCGCGCGTAATTGAAATTTTGAA
CCGCCGGAATAAAAACAATCCGGTGCTGATCGGTGAGCCGGGCGTCGGTAAAACGGCTATTGCAGAAGGCCTCGCGCTTA
AAATCGCTGAAGGCGATGTGCCGAACAAATTGAAAAACAAAGAGCTTTACCTGCTTGACGTCGCTTCTCTCGTCGCCAAT
ACAGGGATCAGAGGCCAGTTTGAAGAAAGAATGAAACAATTAATGAACGAATTAAAAGAACGGAAAAACGTCATCTTGTT
CATTGACGAGATTCACCTTCTTGTAGGCGCCGGCTCTGCCGAGGGATCAATGGATGCCGGGAACATCCTGAAACCGGCGC
TTGCCAGAGGCGAGCTGCAGGTCATCGGGGCGACAACGCTGAAAGAGTACCGCCAGATTGAAAAAGATGCGGCGCTTGAA
AGACGCTTCCAGCCTGTCATGGTTCACGAGCCTACAATAGAACAAGCCATCAGCATTCTGGAAGGCATTAAAGATAAATA
TGAGAAATACCACGGCGTCACATTCAGCAATGAAGCGATAAATGCCTGTGTTACGCTTTCATCCCGTTACATTCAGGACC
GCCACCTGCCGGATAAAGCGATCGACTTATTAGATGAAGCCGGCTCTAAGGCGAACCTGGAGCTTGATACCGTAAATGAA
GAAGATGCGGCTGAGAGACTCAAAGCAATTGACGCGGAGAAAACAAAAGCGCTTCATGAAGAAAATTATGAGCTTGCCGC
GAAACTGCGTGATGAAGAAGCGGCGTTGGAAAAGCAGCTGGCGCATGAAACACCGGCCTCTTCACCTGAAATCGGGGCGG
AGCATATTCAAGCCATCATTGAACAGAAAACAGGCATTCCGGTCGGAAAACTCCAGGCTGATGAACAAGTCAAAATGAAG
GAACTTGAAGAGCATCTTCATCAGCGCGTCATCGGTCAGGAAAAAGCCGTGAAAAAAGTGGCGAAAGCGGTCAGACGAAG
CCGCGCCGGCTTAAAATCCAAAAACAGACCTGTCGGCTCCTTCCTCTTTGTCGGACCGACCGGAGTCGGAAAAACCGAGT
TATCAAAGACACTCGCAGATGAACTGTTCGGTACAAAAGATTCCATTATCCGTCTCGATATGAGTGAATACATGGAAAAA
CACGCCGTATCAAAAATCATCGGTTCACCGCCGGGATATGTCGGACACGATGAAGCCGGTCAATTAACGGAAAAAGTGCG
CCGCAATCCGTACAGCATCGTGCTGCTCGATGAGATTGAAAAAGCCCACCCTGATGTCCAGCATATGTTTCTGCAGATTA
TGGAGGACGGCCGTCTGACAGACAGCCAGGGCAGAACGGTCAGCTTCAAAGATACCGTTTTGATTATGACAAGCAACGCT
GGAACCGGCGAAAAACAGGTGAAAGTCGGATTCCAATCTGAAGAGAGCGTCCTGGAAGAACAAACGCTGATTGATTCACT
GAGCGCGTTCTTCAAACCGGAGTTTTTAAACCGGTTTGACAGCATCATTGAATTCCAATCCCTGAAAAAAGAACATCTCG
TGAAAATCGTCAGCCTGCTTCTCACAGAGCTTGAAGACACATTAAAAGAGCGCGGCATCAGCCTGAGCGTAACGGACGAA
GCGAAAGAAAAAATCGCTGAAATCGGCTACCACCCAGCTTTCGGAGCACGCCCGCTCAGAAGAACCATCCAAGAATTGAT
CGAGGACGAAATGACAGATCTCTTATTGGATAACAACGAAATAAAAGCCTTCCATGTCGTAATGGAAGAGGACGGCATCA
AAGTCCGGGCACAATAA

Upstream 100 bases:

>100_bases
TCAGAGTATACTATTAATCAAAGAAGGTCAAAGTTAAGACCTTATTTTCTAATCCATTTGGTCAAAGATGATCAAACATT
TTTCGAGGAGGTTTTGGCAA

Downstream 100 bases:

>100_bases
GAAAAAAGCGGTTCCCTCCTCGCGGGGAGCCGCTTTTTTGTTTCTTTCTCCATGCCGGAAGGGAGAAACTGTGAAGAATT
CGGGAACAATTTCTCAATCA

Product: ClpE

Products: NA

Alternate protein names: ATPase ClpE; Heat shock protein HSP1 [H]

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MRCQHCQKNEATIRLNMQVNSIQKQFVLCETCYTELTRKPAMSMGPQSFGFPFEQGFAPNQNKQQAGPEKGLLDELAQNV
TNGAKAGLIDPVIGRDHEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVAN
TGIRGQFEERMKQLMNELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALE
RRFQPVMVHEPTIEQAISILEGIKDKYEKYHGVTFSNEAINACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLELDTVNE
EDAAERLKAIDAEKTKALHEENYELAAKLRDEEAALEKQLAHETPASSPEIGAEHIQAIIEQKTGIPVGKLQADEQVKMK
ELEEHLHQRVIGQEKAVKKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDSIIRLDMSEYMEK
HAVSKIIGSPPGYVGHDEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVLIMTSNA
GTGEKQVKVGFQSEESVLEEQTLIDSLSAFFKPEFLNRFDSIIEFQSLKKEHLVKIVSLLLTELEDTLKERGISLSVTDE
AKEKIAEIGYHPAFGARPLRRTIQELIEDEMTDLLLDNNEIKAFHVVMEEDGIKVRAQ

Sequences:

>Translated_698_residues
MRCQHCQKNEATIRLNMQVNSIQKQFVLCETCYTELTRKPAMSMGPQSFGFPFEQGFAPNQNKQQAGPEKGLLDELAQNV
TNGAKAGLIDPVIGRDHEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVAN
TGIRGQFEERMKQLMNELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALE
RRFQPVMVHEPTIEQAISILEGIKDKYEKYHGVTFSNEAINACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLELDTVNE
EDAAERLKAIDAEKTKALHEENYELAAKLRDEEAALEKQLAHETPASSPEIGAEHIQAIIEQKTGIPVGKLQADEQVKMK
ELEEHLHQRVIGQEKAVKKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDSIIRLDMSEYMEK
HAVSKIIGSPPGYVGHDEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVLIMTSNA
GTGEKQVKVGFQSEESVLEEQTLIDSLSAFFKPEFLNRFDSIIEFQSLKKEHLVKIVSLLLTELEDTLKERGISLSVTDE
AKEKIAEIGYHPAFGARPLRRTIQELIEDEMTDLLLDNNEIKAFHVVMEEDGIKVRAQ
>Mature_698_residues
MRCQHCQKNEATIRLNMQVNSIQKQFVLCETCYTELTRKPAMSMGPQSFGFPFEQGFAPNQNKQQAGPEKGLLDELAQNV
TNGAKAGLIDPVIGRDHEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVAN
TGIRGQFEERMKQLMNELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALE
RRFQPVMVHEPTIEQAISILEGIKDKYEKYHGVTFSNEAINACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLELDTVNE
EDAAERLKAIDAEKTKALHEENYELAAKLRDEEAALEKQLAHETPASSPEIGAEHIQAIIEQKTGIPVGKLQADEQVKMK
ELEEHLHQRVIGQEKAVKKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDSIIRLDMSEYMEK
HAVSKIIGSPPGYVGHDEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVLIMTSNA
GTGEKQVKVGFQSEESVLEEQTLIDSLSAFFKPEFLNRFDSIIEFQSLKKEHLVKIVSLLLTELEDTLKERGISLSVTDE
AKEKIAEIGYHPAFGARPLRRTIQELIEDEMTDLLLDNNEIKAFHVVMEEDGIKVRAQ

Specific function: ATPase essential both for efficient CtsR-dependent gene derepression during heat stress and for rerepression. Together with ClpP, degrades the global regulator CtsR after heat shock. Is also involved in disaggregation of heat-denatured proteins. Has thus

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=307, Percent_Identity=36.1563517915309, Blast_Score=186, Evalue=7e-47,
Organism=Escherichia coli, GI1788943, Length=711, Percent_Identity=41.7721518987342, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1787109, Length=628, Percent_Identity=41.8789808917197, Blast_Score=498, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6320464, Length=704, Percent_Identity=41.4772727272727, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6323002, Length=366, Percent_Identity=39.6174863387978, Blast_Score=243, Evalue=7e-65,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 77770; Mature: 77770

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50151 UVR ; PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCQHCQKNEATIRLNMQVNSIQKQFVLCETCYTELTRKPAMSMGPQSFGFPFEQGFAPN
CCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHCCCCCC
QNKQQAGPEKGLLDELAQNVTNGAKAGLIDPVIGRDHEVARVIEILNRRNKNNPVLIGEP
CCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECC
GVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVANTGIRGQFEERMKQLMNELKE
CCCHHHHHCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHH
RKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALE
HCCEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
RRFQPVMVHEPTIEQAISILEGIKDKYEKYHGVTFSNEAINACVTLSSRYIQDRHLPDKA
HCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
IDLLDEAGSKANLELDTVNEEDAAERLKAIDAEKTKALHEENYELAAKLRDEEAALEKQL
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AHETPASSPEIGAEHIQAIIEQKTGIPVGKLQADEQVKMKELEEHLHQRVIGQEKAVKKV
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHH
AKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDSIIRLDMSEYMEK
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHEEEECHHHHHHH
HAVSKIIGSPPGYVGHDEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLT
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCC
DSQGRTVSFKDTVLIMTSNAGTGEKQVKVGFQSEESVLEEQTLIDSLSAFFKPEFLNRFD
CCCCCEEEECCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHH
SIIEFQSLKKEHLVKIVSLLLTELEDTLKERGISLSVTDEAKEKIAEIGYHPAFGARPLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHHH
RTIQELIEDEMTDLLLDNNEIKAFHVVMEEDGIKVRAQ
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEECC
>Mature Secondary Structure
MRCQHCQKNEATIRLNMQVNSIQKQFVLCETCYTELTRKPAMSMGPQSFGFPFEQGFAPN
CCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHCCCCCC
QNKQQAGPEKGLLDELAQNVTNGAKAGLIDPVIGRDHEVARVIEILNRRNKNNPVLIGEP
CCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECC
GVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVANTGIRGQFEERMKQLMNELKE
CCCHHHHHCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHH
RKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALE
HCCEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
RRFQPVMVHEPTIEQAISILEGIKDKYEKYHGVTFSNEAINACVTLSSRYIQDRHLPDKA
HCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
IDLLDEAGSKANLELDTVNEEDAAERLKAIDAEKTKALHEENYELAAKLRDEEAALEKQL
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AHETPASSPEIGAEHIQAIIEQKTGIPVGKLQADEQVKMKELEEHLHQRVIGQEKAVKKV
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHH
AKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDSIIRLDMSEYMEK
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHEEEECHHHHHHH
HAVSKIIGSPPGYVGHDEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLT
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCC
DSQGRTVSFKDTVLIMTSNAGTGEKQVKVGFQSEESVLEEQTLIDSLSAFFKPEFLNRFD
CCCCCEEEECCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHH
SIIEFQSLKKEHLVKIVSLLLTELEDTLKERGISLSVTDEAKEKIAEIGYHPAFGARPLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHHH
RTIQELIEDEMTDLLLDNNEIKAFHVVMEEDGIKVRAQ
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]