Click here to switch to the map view.

The map label for this gene is pit [H]

Identifier: 154685702

GI number: 154685702

Start: 1229278

End: 1230276

Strand: Reverse

Name: pit [H]

Synonym: RBAM_012690

Alternate gene names: 154685702

Gene position: 1230276-1229278 (Counterclockwise)

Preceding gene: 154685703

Following gene: 154685701

Centisome position: 31.4

GC content: 47.65

Gene sequence:

>999_bases
ATGGATACTCTATTACTTTTAACGATATTAATTGTCATATGCGCTTTGGTATTTGACTTCATTAACGGATTTCACGATAC
CGCAAACGCAATCGCCACCTCAGTTTCGACAAAAGCGCTGAAGCCGCGGCACGCAATCGTCATGGCCGCCATCATGAACT
TTCTCGGAGCGATGACATTTACCGGTGTGGCGAAAACCATTACAAAGGACATTGTGAATCCTTATACATTAGACAACGGG
TCAGTTGTCATTCTGGCGGCTTTACTCGCCGCAATCGTCTGGAACTTGATCACATGGTATTTCGGCATTCCGAGCAGCTC
TTCACATGCGATTATCGGAGCGATCGCCGGAGCGGCCATTGCAGCGTCAGGTTTTGCAACGCTTAATTATAAAGGGTTCA
TTAAAATTATCGAAGCACTTATTTTATCACCGATCATCGCCTTTGTTCTCGGCTTTATCGTATATTCGATCGTGAAGCTG
ATTTTTAAAGACAGCAATCTGGCGAAAACAAATAAACGCTTCCGCAGAGTGCAGATTTTGACGGCTGCCATTCAGTCCTA
TACACACGGAACGAACGACGCGCAAAAAGCGATGGGTATCATTACGATGGCGCTTATGACGGCCAATTTACATAAAAGTG
ATGCCATTCCTTTTTGGGTGCAGCTCTCTTGTGCCCTGGCAATGGGTCTCGGCACATCCATCGGCGGCTGGAAAATCATT
AAAACCGTCGGCGGAAAAATTATGAAAATCCGTCCGGTAAACGGCGTATCTGCCGACTTAACCGGCGCAGCCATTATTTT
CGGGGCCACGTTTATTCATCTCCCTGTCAGCACGACGCACGTCATTTCGTCTTCCATTCTCGGTGTGGGCGCTTCCCACC
GCGTAAAAGGCGTAAACTGGGGAACGGCTAAACGGATGCTCATTACATGGGTCATCACCCTGCCGATTGCAGGTACACTC
GGCGCCATCGCCTTTTTCATCCTAGATCTCTTTTTCTGA

Upstream 100 bases:

>100_bases
CGAGATTTACGAAACGCTTGAGGAAATTGCCGACTCTTGCCAAAGCGTTGCCAATAACCTTGAAACCATTATTATGAAAA
ACGCGTAACGAGGGGTTATA

Downstream 100 bases:

>100_bases
TGACATCCGCTTCTTTACAGAAGCGGTTTTTTTTGTGACAGGAAAATGATGTCATGACATAGAATGAACATAGCAGACCA
TTTTTCAAATTGTGAGGGAT

Product: Pit

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDTLLLLTILIVICALVFDFINGFHDTANAIATSVSTKALKPRHAIVMAAIMNFLGAMTFTGVAKTITKDIVNPYTLDNG
SVVILAALLAAIVWNLITWYFGIPSSSSHAIIGAIAGAAIAASGFATLNYKGFIKIIEALILSPIIAFVLGFIVYSIVKL
IFKDSNLAKTNKRFRRVQILTAAIQSYTHGTNDAQKAMGIITMALMTANLHKSDAIPFWVQLSCALAMGLGTSIGGWKII
KTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPIAGTL
GAIAFFILDLFF

Sequences:

>Translated_332_residues
MDTLLLLTILIVICALVFDFINGFHDTANAIATSVSTKALKPRHAIVMAAIMNFLGAMTFTGVAKTITKDIVNPYTLDNG
SVVILAALLAAIVWNLITWYFGIPSSSSHAIIGAIAGAAIAASGFATLNYKGFIKIIEALILSPIIAFVLGFIVYSIVKL
IFKDSNLAKTNKRFRRVQILTAAIQSYTHGTNDAQKAMGIITMALMTANLHKSDAIPFWVQLSCALAMGLGTSIGGWKII
KTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPIAGTL
GAIAFFILDLFF
>Mature_332_residues
MDTLLLLTILIVICALVFDFINGFHDTANAIATSVSTKALKPRHAIVMAAIMNFLGAMTFTGVAKTITKDIVNPYTLDNG
SVVILAALLAAIVWNLITWYFGIPSSSSHAIIGAIAGAAIAASGFATLNYKGFIKIIEALILSPIIAFVLGFIVYSIVKL
IFKDSNLAKTNKRFRRVQILTAAIQSYTHGTNDAQKAMGIITMALMTANLHKSDAIPFWVQLSCALAMGLGTSIGGWKII
KTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPIAGTL
GAIAFFILDLFF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=162, Percent_Identity=31.4814814814815, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=30.2631578947368, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789360, Length=219, Percent_Identity=35.1598173515982, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=33.6405529953917, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25146401, Length=161, Percent_Identity=32.9192546583851, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=160, Percent_Identity=30.625, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17539280, Length=166, Percent_Identity=29.5180722891566, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566716, Length=177, Percent_Identity=28.2485875706215, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=166, Percent_Identity=28.3132530120482, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=154, Percent_Identity=31.8181818181818, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35293; Mature: 35293

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLLLLTILIVICALVFDFINGFHDTANAIATSVSTKALKPRHAIVMAAIMNFLGAMTF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVNPYTLDNGSVVILAALLAAIVWNLITWYFGIPSSSSHAIIGAIAGAAI
HHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AASGFATLNYKGFIKIIEALILSPIIAFVLGFIVYSIVKLIFKDSNLAKTNKRFRRVQIL
HHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TAAIQSYTHGTNDAQKAMGIITMALMTANLHKSDAIPFWVQLSCALAMGLGTSIGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
KTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNW
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCC
GTAKRMLITWVITLPIAGTLGAIAFFILDLFF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDTLLLLTILIVICALVFDFINGFHDTANAIATSVSTKALKPRHAIVMAAIMNFLGAMTF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVNPYTLDNGSVVILAALLAAIVWNLITWYFGIPSSSSHAIIGAIAGAAI
HHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AASGFATLNYKGFIKIIEALILSPIIAFVLGFIVYSIVKLIFKDSNLAKTNKRFRRVQIL
HHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TAAIQSYTHGTNDAQKAMGIITMALMTANLHKSDAIPFWVQLSCALAMGLGTSIGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
KTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNW
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCC
GTAKRMLITWVITLPIAGTLGAIAFFILDLFF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]