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The map label for this gene is yfjF [H]

Identifier: 154685643

GI number: 154685643

Start: 1177779

End: 1179191

Strand: Reverse

Name: yfjF [H]

Synonym: RBAM_012100

Alternate gene names: 154685643

Gene position: 1179191-1177779 (Counterclockwise)

Preceding gene: 154685644

Following gene: 154685635

Centisome position: 30.09

GC content: 45.29

Gene sequence:

>1413_bases
ATGTCAACACATAAAAGAAAGGAACTTCGGGTGCCAAAAGAAGTTCTCACAGCCGCCTGGGCTATTGCACTGGGAGCAAT
CGCTCCAATGCTTGACTCTACGATGGTAAACATCGCCATCGGCAAACTGACCAAAGACTTCAACACGACATTGGATACCA
TTCAGTGGTCCGTTACAGGCTATGTCTTAGCACTTGCGATTGCAGTACCCGTATCCGGCTGGCTTATGAATAAATATAAC
GGTAAGAAAATCTTCATCGGCTCAGTCATCGCCTTTGGTGTCATTTCTGTTTTTGCGGGGATGAGCCGTGACGTTTCCAG
CTTCATCTTCTTCCGTTTGCTTCAAGGATTCAGCGCCGGCATTATAACTCCGCTTATGTCAACACTCTTGGTTAAAACGG
CAGGTCCGGAAAACCTGGGAAAAGTGATGGCAATCGTCAGCACCCCTATGATCTTCGGCCCGATCTTAGGACCTGTCATC
GGAGGCTTCATCGTCCAAGGCGCTTCATGGCATTGGATTTTTTACATCAACGTGTTCATCGTTTTGATTGCTGCACCGCT
TATGATGAAGACCATTCCAGACTTTGAACCATTCAACAAAGATCGTAAACTCGATATTTTCGGGATTATCAATTTGTCAT
TAATGAGCGCGGCATTGATTTACGGTATTACGAAAGCGGCGGATCATGCATCATTTAACAATAGCGACACCATTTTATGG
GCGGGCATCGGTCTTGTTTCAGCAGTCATTTATCTTGTATATAATCTGATCAAGAAAAACCAAACCGTGCTGCCTATGAA
TTTGTTTGCAAACAAGAGCTTCTTATCATCTTCGATCGGGTTGTTCTTAGCGAATGTGGCCATCATGGGGCCGATGTTAA
TACTTCCGCTTTTTTTTCAAAACATTCGTCAGTTCACGGCAGTCGGAGCAGCACTCGCTTTAATCCCTCAAGGAGTCGGA
ATGCTCGTGACTCGTCCGCTGATCGGAAAAATGATTGATAAGATCGGTGCGAAATACGTGGTTACGGTCAGTCTTGTTCT
TTCGCTGATCGGATCGATCCCGCTTATTTTTATCACCGATAACACAAGTATGATTTGGATTTCCGTCGTATTATTTATCC
GCGGCACCAGTTTTGGAGGAATTATGCTCCCATTGATGAGTGACGCTTATACAGGCCTTGACAGCAAACAGCTTCCGGAA
GCAGGTGTCGGTATTCATATGATTGAAAACCTCGGTTCCAGCTTTGGGTCAGCTGTGATCGCAACCGTTACCGCAACTGT
CATACAAGGATTGCATCCGACGATTTCGAACAGTTTAAAAGGCTACCATGCCGGATTCTTAGTATCCGTCATCATCCTTG
CGGCCATCTTCATTCCAAGTCTGTTCCTTACCCATAAAAAGACTGCGATATAA

Upstream 100 bases:

>100_bases
CTGCCTTTTTATATCCCTCTTATTGTTGACAAAGAAACAAAATTACTCTATTATTTTGTTGACAAGGAAACAAAGACAGC
AATTTTGAGAGGAGAGTAAT

Downstream 100 bases:

>100_bases
AGAACTTTTTAAAGTAAAAAACCCTTGCACAGCAAGGGTTTTAGTGAATGAAACTGATGATGGTTGCCGGCAAAGTCAGC
CAACAATAGACAAAGCCTTT

Product: YfjF

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSTHKRKELRVPKEVLTAAWAIALGAIAPMLDSTMVNIAIGKLTKDFNTTLDTIQWSVTGYVLALAIAVPVSGWLMNKYN
GKKIFIGSVIAFGVISVFAGMSRDVSSFIFFRLLQGFSAGIITPLMSTLLVKTAGPENLGKVMAIVSTPMIFGPILGPVI
GGFIVQGASWHWIFYINVFIVLIAAPLMMKTIPDFEPFNKDRKLDIFGIINLSLMSAALIYGITKAADHASFNNSDTILW
AGIGLVSAVIYLVYNLIKKNQTVLPMNLFANKSFLSSSIGLFLANVAIMGPMLILPLFFQNIRQFTAVGAALALIPQGVG
MLVTRPLIGKMIDKIGAKYVVTVSLVLSLIGSIPLIFITDNTSMIWISVVLFIRGTSFGGIMLPLMSDAYTGLDSKQLPE
AGVGIHMIENLGSSFGSAVIATVTATVIQGLHPTISNSLKGYHAGFLVSVIILAAIFIPSLFLTHKKTAI

Sequences:

>Translated_470_residues
MSTHKRKELRVPKEVLTAAWAIALGAIAPMLDSTMVNIAIGKLTKDFNTTLDTIQWSVTGYVLALAIAVPVSGWLMNKYN
GKKIFIGSVIAFGVISVFAGMSRDVSSFIFFRLLQGFSAGIITPLMSTLLVKTAGPENLGKVMAIVSTPMIFGPILGPVI
GGFIVQGASWHWIFYINVFIVLIAAPLMMKTIPDFEPFNKDRKLDIFGIINLSLMSAALIYGITKAADHASFNNSDTILW
AGIGLVSAVIYLVYNLIKKNQTVLPMNLFANKSFLSSSIGLFLANVAIMGPMLILPLFFQNIRQFTAVGAALALIPQGVG
MLVTRPLIGKMIDKIGAKYVVTVSLVLSLIGSIPLIFITDNTSMIWISVVLFIRGTSFGGIMLPLMSDAYTGLDSKQLPE
AGVGIHMIENLGSSFGSAVIATVTATVIQGLHPTISNSLKGYHAGFLVSVIILAAIFIPSLFLTHKKTAI
>Mature_469_residues
STHKRKELRVPKEVLTAAWAIALGAIAPMLDSTMVNIAIGKLTKDFNTTLDTIQWSVTGYVLALAIAVPVSGWLMNKYNG
KKIFIGSVIAFGVISVFAGMSRDVSSFIFFRLLQGFSAGIITPLMSTLLVKTAGPENLGKVMAIVSTPMIFGPILGPVIG
GFIVQGASWHWIFYINVFIVLIAAPLMMKTIPDFEPFNKDRKLDIFGIINLSLMSAALIYGITKAADHASFNNSDTILWA
GIGLVSAVIYLVYNLIKKNQTVLPMNLFANKSFLSSSIGLFLANVAIMGPMLILPLFFQNIRQFTAVGAALALIPQGVGM
LVTRPLIGKMIDKIGAKYVVTVSLVLSLIGSIPLIFITDNTSMIWISVVLFIRGTSFGGIMLPLMSDAYTGLDSKQLPEA
GVGIHMIENLGSSFGSAVIATVTATVIQGLHPTISNSLKGYHAGFLVSVIILAAIFIPSLFLTHKKTAI

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=427, Percent_Identity=26.2295081967213, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1788710, Length=430, Percent_Identity=27.2093023255814, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1790195, Length=451, Percent_Identity=25.0554323725055, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788392, Length=372, Percent_Identity=27.1505376344086, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081983, Length=408, Percent_Identity=24.2647058823529, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081723, Length=156, Percent_Identity=28.8461538461538, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=333, Percent_Identity=22.8228228228228, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50462; Mature: 50331

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTHKRKELRVPKEVLTAAWAIALGAIAPMLDSTMVNIAIGKLTKDFNTTLDTIQWSVTG
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YVLALAIAVPVSGWLMNKYNGKKIFIGSVIAFGVISVFAGMSRDVSSFIFFRLLQGFSAG
HHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCH
IITPLMSTLLVKTAGPENLGKVMAIVSTPMIFGPILGPVIGGFIVQGASWHWIFYINVFI
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
VLIAAPLMMKTIPDFEPFNKDRKLDIFGIINLSLMSAALIYGITKAADHASFNNSDTILW
HHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
AGIGLVSAVIYLVYNLIKKNQTVLPMNLFANKSFLSSSIGLFLANVAIMGPMLILPLFFQ
EHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIRQFTAVGAALALIPQGVGMLVTRPLIGKMIDKIGAKYVVTVSLVLSLIGSIPLIFITD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEC
NTSMIWISVVLFIRGTSFGGIMLPLMSDAYTGLDSKQLPEAGVGIHMIENLGSSFGSAVI
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ATVTATVIQGLHPTISNSLKGYHAGFLVSVIILAAIFIPSLFLTHKKTAI
HHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STHKRKELRVPKEVLTAAWAIALGAIAPMLDSTMVNIAIGKLTKDFNTTLDTIQWSVTG
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YVLALAIAVPVSGWLMNKYNGKKIFIGSVIAFGVISVFAGMSRDVSSFIFFRLLQGFSAG
HHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCH
IITPLMSTLLVKTAGPENLGKVMAIVSTPMIFGPILGPVIGGFIVQGASWHWIFYINVFI
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
VLIAAPLMMKTIPDFEPFNKDRKLDIFGIINLSLMSAALIYGITKAADHASFNNSDTILW
HHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
AGIGLVSAVIYLVYNLIKKNQTVLPMNLFANKSFLSSSIGLFLANVAIMGPMLILPLFFQ
EHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIRQFTAVGAALALIPQGVGMLVTRPLIGKMIDKIGAKYVVTVSLVLSLIGSIPLIFITD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEC
NTSMIWISVVLFIRGTSFGGIMLPLMSDAYTGLDSKQLPEAGVGIHMIENLGSSFGSAVI
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ATVTATVIQGLHPTISNSLKGYHAGFLVSVIILAAIFIPSLFLTHKKTAI
HHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]