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The map label for this gene is yjbJ [H]

Identifier: 154685592

GI number: 154685592

Start: 1135845

End: 1136531

Strand: Reverse

Name: yjbJ [H]

Synonym: RBAM_011580

Alternate gene names: 154685592

Gene position: 1136531-1135845 (Counterclockwise)

Preceding gene: 154685593

Following gene: 154685591

Centisome position: 29.0

GC content: 53.28

Gene sequence:

>687_bases
GTGAATATCACGAATTGGGCGGCCCTTCTCCAGCTGCAATCAATGGCGCTGCAATCAATCTCTCATACAAGCACGGCTTC
AGGCGGACAATCTTCAGAAAGCCCGCTTGCAAATTTCAGCGCGCTTCTCAGCCAGTATACGGACACAGCCGTCCGGCAGC
AGCCGGAATCAGGAATTTTAAACGGTGTGCGGACCGCTCACGCTTCATATTCACCGAGAATTCCCGTTTCATCCGGCTCC
GCGGCTGCTTCACAGACCGCTTCCCCGGGCATAAAGTCTCCGGATGCGGCTTCTGATATCAGCACCGGAAACTTTTCAAT
AGACAGCGCCATTCAGAAAGCGGCCGAAAAATACGGAGTGGACGAAAAGCTGATCCGCGCGGTTATTAAACAGGAATCAG
GCTTTGATCCGAAAGCGGTCAGCGGTGCGGGGGCAATGGGACTGATGCAGCTCATGCCTTCCACGGCAGAATCACTCGGG
GTGTCAAACCCGCTTGATCCCGTCCAGAACGCTGAGGGCGGCACAAAGTACTTAAAGCAGATGCTCACGAAATACGACGG
AAATGTATCTCTGGCGTTAGCCGCCTACAACGCAGGCCCCGGCAACGTAGATCGGTACGGAGGCATTCCGCCGTTTAAGG
AAACACAGCAATATGTCAAAAACATAACCAAACAGTATTACGCGTAA

Upstream 100 bases:

>100_bases
TTTGAACAATTGCTCAAGCAATTCGGCATCCCAGAGCGGATCACGAAAAATAAAATCCAGCGTTTTTATGAACAGAAACA
AAAAAAGGTGGAATGACACA

Downstream 100 bases:

>100_bases
ACCAGAGAGGCTTCCTTCGCCCTCTGGTTTTTTTGCGGAGTGAAATCTTACACATGAATGAAGGCGATGTGATATGATAT
GAAATACAAGCGCCTGACAG

Product: YjbJ

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MNITNWAALLQLQSMALQSISHTSTASGGQSSESPLANFSALLSQYTDTAVRQQPESGILNGVRTAHASYSPRIPVSSGS
AAASQTASPGIKSPDAASDISTGNFSIDSAIQKAAEKYGVDEKLIRAVIKQESGFDPKAVSGAGAMGLMQLMPSTAESLG
VSNPLDPVQNAEGGTKYLKQMLTKYDGNVSLALAAYNAGPGNVDRYGGIPPFKETQQYVKNITKQYYA

Sequences:

>Translated_228_residues
MNITNWAALLQLQSMALQSISHTSTASGGQSSESPLANFSALLSQYTDTAVRQQPESGILNGVRTAHASYSPRIPVSSGS
AAASQTASPGIKSPDAASDISTGNFSIDSAIQKAAEKYGVDEKLIRAVIKQESGFDPKAVSGAGAMGLMQLMPSTAESLG
VSNPLDPVQNAEGGTKYLKQMLTKYDGNVSLALAAYNAGPGNVDRYGGIPPFKETQQYVKNITKQYYA
>Mature_228_residues
MNITNWAALLQLQSMALQSISHTSTASGGQSSESPLANFSALLSQYTDTAVRQQPESGILNGVRTAHASYSPRIPVSSGS
AAASQTASPGIKSPDAASDISTGNFSIDSAIQKAAEKYGVDEKLIRAVIKQESGFDPKAVSGAGAMGLMQLMPSTAESLG
VSNPLDPVQNAEGGTKYLKQMLTKYDGNVSLALAAYNAGPGNVDRYGGIPPFKETQQYVKNITKQYYA

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=119, Percent_Identity=39.4957983193277, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082191, Length=113, Percent_Identity=38.0530973451327, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI171474010, Length=92, Percent_Identity=39.1304347826087, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23856; Mature: 23856

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITNWAALLQLQSMALQSISHTSTASGGQSSESPLANFSALLSQYTDTAVRQQPESGIL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
NGVRTAHASYSPRIPVSSGSAAASQTASPGIKSPDAASDISTGNFSIDSAIQKAAEKYGV
HHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
DEKLIRAVIKQESGFDPKAVSGAGAMGLMQLMPSTAESLGVSNPLDPVQNAEGGTKYLKQ
CHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHH
MLTKYDGNVSLALAAYNAGPGNVDRYGGIPPFKETQQYVKNITKQYYA
HHHHCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNITNWAALLQLQSMALQSISHTSTASGGQSSESPLANFSALLSQYTDTAVRQQPESGIL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
NGVRTAHASYSPRIPVSSGSAAASQTASPGIKSPDAASDISTGNFSIDSAIQKAAEKYGV
HHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
DEKLIRAVIKQESGFDPKAVSGAGAMGLMQLMPSTAESLGVSNPLDPVQNAEGGTKYLKQ
CHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHH
MLTKYDGNVSLALAAYNAGPGNVDRYGGIPPFKETQQYVKNITKQYYA
HHHHCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]