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The map label for this gene is yitJ [H]

Identifier: 154685536

GI number: 154685536

Start: 1080047

End: 1081888

Strand: Reverse

Name: yitJ [H]

Synonym: RBAM_011020

Alternate gene names: 154685536

Gene position: 1081888-1080047 (Counterclockwise)

Preceding gene: 154685537

Following gene: 154685535

Centisome position: 27.61

GC content: 50.22

Gene sequence:

>1842_bases
ATGGGACTATTACAAGACTTAAAAGAACGGATTTTAATCGGAGACGGTGCGATGGGCACTCTCCTCTATTCCTACGGCAT
TGACAGATGTTTTGAGGAACTGAACGTATCAAAGCCCGAAGAAATCAAGCGGATACATAAGGCTTACGTGGAAGCCGGGG
CAGACATCATCCAGACAAATACGTACGGCGCGAATTTTATTAAGCTCTCAAGATACGGACTTGAGGACGAAACGAAAAAA
ATCAATCAGGAGGCGGTCAGAATCGCGCGCGCTTCCTCAGACGGCGCTTATGTATTGGGAACGATGGGCGGCATCCGCAC
CTTTAACAAAAACGCCTACAGCCTGGAGGAGATTAAACGCAGCTTTCGGGAGCAGCTTTATTTATTGCTGCACGAAGAGC
CGGACGGTCTGCTTCTGGAAACATACTATGATTTAGAAGAGGCGCGCGAAGTGCTGAAGATCGCGAGAAAAGAAACGGAA
CTTCCGATTATGCTGAACGTATCCATGCACGAACAGGGCGTGCTGCAGGACGGAACGCCGCTTTGGGAAGCCCTCAGGTC
CATTGCCGATCTCGGCGCTGACATAGCGGGAATCAATTGCCGGCTCGGCCCGTACCACATGATTGAAGCGCTCACCGAAG
TGCCGATTTTCCGTGACGCTTATTTATCGGTCTATCCGAACAGCAGCCTTCCGTCTTTAGAAGAAGGCCGGCTTGTTTAT
GATACGGACGATGCCTATTTCCGGGAGAGCGCCGTACAGTTCCGCACTCAGGGCGCGCGCATTATCGGCGGCTGCTGCGG
TACGACGCCAAACCATATCAGAGCCATGGCTGAAGCGGTTCGCGGCCTTTCCCCCGTCACTGAGAAAGAAGTGAAAATCA
GACGGGAAGAAGAAGTCATCTCAGTCAGCAACGAACGGACCGAGCCGGGTTTAAACGAAATCGCCGCTCAAAAACGGTCA
ATTATCGTAGAGCTTGATCCGCCAAAAAAATTGAATTTTGACAAATTTCTGCAAGCGGCGGCTGAATTAAAAGAAACCGG
CATAGAAGCGCTGACGCTCGCCGACAATTCATTGGCGACACCGCGGATCAGCAATGCCGCCTGCGGTTCATTGCTGAAAG
AGCGCCTTGATATCCGTTCTCTCGTTCACATTACATGCAGAGACCGCAACATCATCGGCCTTCAGTCACATTTAATGGGG
CTGGATACGCTCGGGCTCACCGACATTTTAGCCATCACGGGCGATCCTTCAAAAATCGGCGACTTTCCGGGGGCTACCTC
CGTCTATGATTTAACGTCCTTTGACTTGATCAGGCTGATTAAACAATTCAACGAGGGCATTTCTTTATCAGGGAAACCGC
TCGGCAAGAAAACGAACTTTTCCGTAGCCGGAGCATTTAATCCGAATGTCCGCCACCTTGATAAAGCAGTAAAACGGCTG
GAGAAAAAAATCGAGTGCGGAGCTGACTACTTCGTGTCCCAGCCTGTCTACTCGGAGCAGCAGCTCATTGACATTCATCA
TGAAACGAAACATCTCAAAACACCTGTGTATATCGGAATCATGCCGTTAACGAGCAGCCGCAACGCAGAGTTCATTCATC
ATGAAATCCCGGGCATTAAGCTGTCAGACAGCATCCGAGAAAAAATGGCGCTCGCCGGCGAAGACAAGCGAAAGCAGAAA
GCCGAAGGCCTCGCCATCGCCAAGTCGCTTCTCGACACGGCGTGCGAACTGTTTAACGGAATTTATTTAATCACCCCGTT
TCTCCGTTCTGATTTGACGTCAGAGCTTGCAGCTTATATTAAGCAAAAGGATGAAAAGCGGGCTGATGTATATTTGCATT
GA

Upstream 100 bases:

>100_bases
GAAGATAAGAAGAGAGACAAATGATTACAAAGTCTTCTTCTTAAGAAGACTTTTTTTATTTCTCTTTTTTCCTTGCTGAA
TGTGACGAAGGAGGCAAACA

Downstream 100 bases:

>100_bases
CGCGGAAGAGGCCGCCTGATATGACAGGCGGCTTTTTCATGGTACGATACAGAAAAAGAATCAAGCAAAGGAGGATTTTT
TCATGGTTGAAGTAAAACCC

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MGLLQDLKERILIGDGAMGTLLYSYGIDRCFEELNVSKPEEIKRIHKAYVEAGADIIQTNTYGANFIKLSRYGLEDETKK
INQEAVRIARASSDGAYVLGTMGGIRTFNKNAYSLEEIKRSFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETE
LPIMLNVSMHEQGVLQDGTPLWEALRSIADLGADIAGINCRLGPYHMIEALTEVPIFRDAYLSVYPNSSLPSLEEGRLVY
DTDDAYFRESAVQFRTQGARIIGGCCGTTPNHIRAMAEAVRGLSPVTEKEVKIRREEEVISVSNERTEPGLNEIAAQKRS
IIVELDPPKKLNFDKFLQAAAELKETGIEALTLADNSLATPRISNAACGSLLKERLDIRSLVHITCRDRNIIGLQSHLMG
LDTLGLTDILAITGDPSKIGDFPGATSVYDLTSFDLIRLIKQFNEGISLSGKPLGKKTNFSVAGAFNPNVRHLDKAVKRL
EKKIECGADYFVSQPVYSEQQLIDIHHETKHLKTPVYIGIMPLTSSRNAEFIHHEIPGIKLSDSIREKMALAGEDKRKQK
AEGLAIAKSLLDTACELFNGIYLITPFLRSDLTSELAAYIKQKDEKRADVYLH

Sequences:

>Translated_613_residues
MGLLQDLKERILIGDGAMGTLLYSYGIDRCFEELNVSKPEEIKRIHKAYVEAGADIIQTNTYGANFIKLSRYGLEDETKK
INQEAVRIARASSDGAYVLGTMGGIRTFNKNAYSLEEIKRSFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETE
LPIMLNVSMHEQGVLQDGTPLWEALRSIADLGADIAGINCRLGPYHMIEALTEVPIFRDAYLSVYPNSSLPSLEEGRLVY
DTDDAYFRESAVQFRTQGARIIGGCCGTTPNHIRAMAEAVRGLSPVTEKEVKIRREEEVISVSNERTEPGLNEIAAQKRS
IIVELDPPKKLNFDKFLQAAAELKETGIEALTLADNSLATPRISNAACGSLLKERLDIRSLVHITCRDRNIIGLQSHLMG
LDTLGLTDILAITGDPSKIGDFPGATSVYDLTSFDLIRLIKQFNEGISLSGKPLGKKTNFSVAGAFNPNVRHLDKAVKRL
EKKIECGADYFVSQPVYSEQQLIDIHHETKHLKTPVYIGIMPLTSSRNAEFIHHEIPGIKLSDSIREKMALAGEDKRKQK
AEGLAIAKSLLDTACELFNGIYLITPFLRSDLTSELAAYIKQKDEKRADVYLH
>Mature_612_residues
GLLQDLKERILIGDGAMGTLLYSYGIDRCFEELNVSKPEEIKRIHKAYVEAGADIIQTNTYGANFIKLSRYGLEDETKKI
NQEAVRIARASSDGAYVLGTMGGIRTFNKNAYSLEEIKRSFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETEL
PIMLNVSMHEQGVLQDGTPLWEALRSIADLGADIAGINCRLGPYHMIEALTEVPIFRDAYLSVYPNSSLPSLEEGRLVYD
TDDAYFRESAVQFRTQGARIIGGCCGTTPNHIRAMAEAVRGLSPVTEKEVKIRREEEVISVSNERTEPGLNEIAAQKRSI
IVELDPPKKLNFDKFLQAAAELKETGIEALTLADNSLATPRISNAACGSLLKERLDIRSLVHITCRDRNIIGLQSHLMGL
DTLGLTDILAITGDPSKIGDFPGATSVYDLTSFDLIRLIKQFNEGISLSGKPLGKKTNFSVAGAFNPNVRHLDKAVKRLE
KKIECGADYFVSQPVYSEQQLIDIHHETKHLKTPVYIGIMPLTSSRNAEFIHHEIPGIKLSDSIREKMALAGEDKRKQKA
EGLAIAKSLLDTACELFNGIYLITPFLRSDLTSELAAYIKQKDEKRADVYLH

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=321, Percent_Identity=27.4143302180685, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI13162290, Length=317, Percent_Identity=29.6529968454259, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI157266337, Length=328, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1790450, Length=334, Percent_Identity=32.0359281437126, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1786456, Length=301, Percent_Identity=27.5747508305648, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535405, Length=321, Percent_Identity=30.5295950155763, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324982, Length=323, Percent_Identity=25.077399380805, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 68362; Mature: 68231

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLQDLKERILIGDGAMGTLLYSYGIDRCFEELNVSKPEEIKRIHKAYVEAGADIIQTN
CCHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEC
TYGANFIKLSRYGLEDETKKINQEAVRIARASSDGAYVLGTMGGIRTFNKNAYSLEEIKR
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCHHHHHH
SFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETELPIMLNVSMHEQGVLQDGTP
HHHHHHEEEEECCCCCEEEHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCH
LWEALRSIADLGADIAGINCRLGPYHMIEALTEVPIFRDAYLSVYPNSSLPSLEEGRLVY
HHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCHHCCEEEEECCCCCCCCCCCEEEE
DTDDAYFRESAVQFRTQGARIIGGCCGTTPNHIRAMAEAVRGLSPVTEKEVKIRREEEVI
ECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SVSNERTEPGLNEIAAQKRSIIVELDPPKKLNFDKFLQAAAELKETGIEALTLADNSLAT
CCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
PRISNAACGSLLKERLDIRSLVHITCRDRNIIGLQSHLMGLDTLGLTDILAITGDPSKIG
CCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEHHHHHHCCHHCCHHHEEEECCCCHHCC
DFPGATSVYDLTSFDLIRLIKQFNEGISLSGKPLGKKTNFSVAGAFNPNVRHLDKAVKRL
CCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
EKKIECGADYFVSQPVYSEQQLIDIHHETKHLKTPVYIGIMPLTSSRNAEFIHHEIPGIK
HHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHCCCCCE
LSDSIREKMALAGEDKRKQKAEGLAIAKSLLDTACELFNGIYLITPFLRSDLTSELAAYI
ECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHH
KQKDEKRADVYLH
HHHCCCCCCCCCC
>Mature Secondary Structure 
GLLQDLKERILIGDGAMGTLLYSYGIDRCFEELNVSKPEEIKRIHKAYVEAGADIIQTN
CHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEC
TYGANFIKLSRYGLEDETKKINQEAVRIARASSDGAYVLGTMGGIRTFNKNAYSLEEIKR
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCHHHHHH
SFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETELPIMLNVSMHEQGVLQDGTP
HHHHHHEEEEECCCCCEEEHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCH
LWEALRSIADLGADIAGINCRLGPYHMIEALTEVPIFRDAYLSVYPNSSLPSLEEGRLVY
HHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCHHCCEEEEECCCCCCCCCCCEEEE
DTDDAYFRESAVQFRTQGARIIGGCCGTTPNHIRAMAEAVRGLSPVTEKEVKIRREEEVI
ECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SVSNERTEPGLNEIAAQKRSIIVELDPPKKLNFDKFLQAAAELKETGIEALTLADNSLAT
CCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
PRISNAACGSLLKERLDIRSLVHITCRDRNIIGLQSHLMGLDTLGLTDILAITGDPSKIG
CCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEHHHHHHCCHHCCHHHEEEECCCCHHCC
DFPGATSVYDLTSFDLIRLIKQFNEGISLSGKPLGKKTNFSVAGAFNPNVRHLDKAVKRL
CCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
EKKIECGADYFVSQPVYSEQQLIDIHHETKHLKTPVYIGIMPLTSSRNAEFIHHEIPGIK
HHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHCCCCCE
LSDSIREKMALAGEDKRKQKAEGLAIAKSLLDTACELFNGIYLITPFLRSDLTSELAAYI
ECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHH
KQKDEKRADVYLH
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]