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The map label for this gene is lytF [H]

Identifier: 154685397

GI number: 154685397

Start: 940456

End: 941910

Strand: Reverse

Name: lytF [H]

Synonym: RBAM_009630

Alternate gene names: 154685397

Gene position: 941910-940456 (Counterclockwise)

Preceding gene: 154685398

Following gene: 154685395

Centisome position: 24.04

GC content: 46.8

Gene sequence:

>1455_bases
ATGAAAAAAAAGGTAGCGGTCGGTTTGACTGCATCTGCGATCGTCGGGTCTGCGTTAGCTGTAACACCGGCAGAAGCACA
AACGATTAAGGTTAAAAGCGGAGATTCATTATGGAAGCTTGCCCAAAACTACAATACGAGCGTGGATGCCATTACATCAG
CGAATCATCTAAAATCCACCGTTATTTCTATCGGGCAGACACTCAACATTCCTAACGGCAAATCAGATTCCGGCAGCAAA
AAGCCATCTTCAAAGAACAGCGGCACGACTAAAAGCGTTTATACAGTGAAAAGCGGCGACTCGCTGTGGCTGATCGCATC
TGATTTTAAAATGTCCGTACAGGAACTGAAAAAATTGAACGGTCTGACAAGCGACTTAATCCGCGCCGGACAGAAGCTGC
AAGTGACGGGGAAAGTTTCCTCAAGCACCGATCAGAAAAAAACCACGCCGGGCAGCAGCTCAAACAGCAGCAAAAATCAG
TCATCATCTTCCGGGACGTATAAAGTCAAACTCGGCGATTCACTATGGAAGATCGCAAACAGCGTCAATATGACCATTGC
GGAACTTAAAGTATTAAACAACTTAAAAACGGATACGATCTACGTAAACCAAGTCCTGAAAACAAAAGGAACGTCTTCTT
CGGGAAACACGCCGTCAAAAGACAATTCAAACCAAAATAAAAACCAGACGCAAACGTCCTCTTATACAGTAAAAAGCGGA
GACTCACTCTGGAAAATCGCAAACGCATACAATATGACGGTTCAGCAAATCAGAAGCATCAACAAACTGACGTCAGACAT
GCTGCGGGTCGGACAAGTGCTTAAGCTGACCAAAACGTCATCTTCCGGCTCTTCGTCTTCACCGTCAACTCCGAACCAGT
CCGGAGATACAGGGAAAACCACTACATATACCGTGAAAAGCGGGGATTCTTTATGGGTGATCGCCCAAAAGTTCAATGTG
ACCGCCCAGCAGATCCGCGAGAAAAACAATCTGAAAACAGATGTTCTTCAAATCGGACAAAAGCTGACGATTACCGGGCA
GCAGACAAACACGACACCGAATCAGTCCGGCAACACAGGCGGCACAGGTTCAACAAGCGCAAAGATTAATGTAATGATCG
ATGCCGCAAAAGCTCAGCTGGGCGTACCGTATGTATGGGGCGGGACGACGCCGAGAGGCTTTGACTGCAGCGGTTTCATT
TATTACGTTCTCAATAAGGTCACTTCTGTATCCAGACTTTCAGCCGCAGGCTACTGGAATACGATGACATCAGTCAGCCA
GCCGGCGGTCGGTGACTTTGTCTTTTTCTCAACTTATAAAGCCGGACCTTCCCACGTCGGTATTTATCTCGGGAACGGCC
AGTTTATTAATGCCAACGATTCGGGTGTCGTTATTTCCAGCATGAACAATTCCTATTGGAAACAGCGCTACCTCGGCGCA
AAACGTTTCTTCTGA

Upstream 100 bases:

>100_bases
AGACAAGTGCCGATATAAAGATTATCATTACTGATATCCTCACCTTGCGGCTGCAACGAATGGGGATAGTAAAATCATCG
CAGGAAAAGGGAGATTCATT

Downstream 100 bases:

>100_bases
TATCTGACAAGCTGTACGGCCTCAATAAGGCTGTGCAGCTTTTTTTACAGTCTGAAAATATTAATGCTGATCACATGTTT
TGTCACGGGATGGAAATACA

Product: LytF

Products: NA

Alternate protein names: Cell wall-associated polypeptide CWBP49; Gamma-D-glutamate-meso-diaminopimelate muropeptidase lytF [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKKKVAVGLTASAIVGSALAVTPAEAQTIKVKSGDSLWKLAQNYNTSVDAITSANHLKSTVISIGQTLNIPNGKSDSGSK
KPSSKNSGTTKSVYTVKSGDSLWLIASDFKMSVQELKKLNGLTSDLIRAGQKLQVTGKVSSSTDQKKTTPGSSSNSSKNQ
SSSSGTYKVKLGDSLWKIANSVNMTIAELKVLNNLKTDTIYVNQVLKTKGTSSSGNTPSKDNSNQNKNQTQTSSYTVKSG
DSLWKIANAYNMTVQQIRSINKLTSDMLRVGQVLKLTKTSSSGSSSSPSTPNQSGDTGKTTTYTVKSGDSLWVIAQKFNV
TAQQIREKNNLKTDVLQIGQKLTITGQQTNTTPNQSGNTGGTGSTSAKINVMIDAAKAQLGVPYVWGGTTPRGFDCSGFI
YYVLNKVTSVSRLSAAGYWNTMTSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGQFINANDSGVVISSMNNSYWKQRYLGA
KRFF

Sequences:

>Translated_484_residues
MKKKVAVGLTASAIVGSALAVTPAEAQTIKVKSGDSLWKLAQNYNTSVDAITSANHLKSTVISIGQTLNIPNGKSDSGSK
KPSSKNSGTTKSVYTVKSGDSLWLIASDFKMSVQELKKLNGLTSDLIRAGQKLQVTGKVSSSTDQKKTTPGSSSNSSKNQ
SSSSGTYKVKLGDSLWKIANSVNMTIAELKVLNNLKTDTIYVNQVLKTKGTSSSGNTPSKDNSNQNKNQTQTSSYTVKSG
DSLWKIANAYNMTVQQIRSINKLTSDMLRVGQVLKLTKTSSSGSSSSPSTPNQSGDTGKTTTYTVKSGDSLWVIAQKFNV
TAQQIREKNNLKTDVLQIGQKLTITGQQTNTTPNQSGNTGGTGSTSAKINVMIDAAKAQLGVPYVWGGTTPRGFDCSGFI
YYVLNKVTSVSRLSAAGYWNTMTSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGQFINANDSGVVISSMNNSYWKQRYLGA
KRFF
>Mature_484_residues
MKKKVAVGLTASAIVGSALAVTPAEAQTIKVKSGDSLWKLAQNYNTSVDAITSANHLKSTVISIGQTLNIPNGKSDSGSK
KPSSKNSGTTKSVYTVKSGDSLWLIASDFKMSVQELKKLNGLTSDLIRAGQKLQVTGKVSSSTDQKKTTPGSSSNSSKNQ
SSSSGTYKVKLGDSLWKIANSVNMTIAELKVLNNLKTDTIYVNQVLKTKGTSSSGNTPSKDNSNQNKNQTQTSSYTVKSG
DSLWKIANAYNMTVQQIRSINKLTSDMLRVGQVLKLTKTSSSGSSSSPSTPNQSGDTGKTTTYTVKSGDSLWVIAQKFNV
TAQQIREKNNLKTDVLQIGQKLTITGQQTNTTPNQSGNTGGTGSTSAKINVMIDAAKAQLGVPYVWGGTTPRGFDCSGFI
YYVLNKVTSVSRLSAAGYWNTMTSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGQFINANDSGVVISSMNNSYWKQRYLGA
KRFF

Specific function: Cleaves gamma-D-glutamate-meso-diaminopimelate bonds. Cell wall hydrolase involved in cell autolysis [H]

COG id: COG1388

COG function: function code M; FOG: LysM repeat

Gene ontology:

Cell location: Secreted, cell wall. Note=LysM repeats are thought to be involved in peptidoglycan binding [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=118, Percent_Identity=38.9830508474576, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786421, Length=125, Percent_Identity=36.8, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788501, Length=106, Percent_Identity=42.4528301886792, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788001, Length=118, Percent_Identity=37.2881355932203, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559362, Length=258, Percent_Identity=27.1317829457364, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 51592; Mature: 51592

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVAVGLTASAIVGSALAVTPAEAQTIKVKSGDSLWKLAQNYNTSVDAITSANHLKST
CCCEEEEEEEHHHHHCCCEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHH
VISIGQTLNIPNGKSDSGSKKPSSKNSGTTKSVYTVKSGDSLWLIASDFKMSVQELKKLN
HHHHCCEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHC
GLTSDLIRAGQKLQVTGKVSSSTDQKKTTPGSSSNSSKNQSSSSGTYKVKLGDSLWKIAN
CCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHH
SVNMTIAELKVLNNLKTDTIYVNQVLKTKGTSSSGNTPSKDNSNQNKNQTQTSSYTVKSG
CCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
DSLWKIANAYNMTVQQIRSINKLTSDMLRVGQVLKLTKTSSSGSSSSPSTPNQSGDTGKT
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCE
TTYTVKSGDSLWVIAQKFNVTAQQIREKNNLKTDVLQIGQKLTITGQQTNTTPNQSGNTG
EEEEEECCCEEEEEEEECCCHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCC
GTGSTSAKINVMIDAAKAQLGVPYVWGGTTPRGFDCSGFIYYVLNKVTSVSRLSAAGYWN
CCCCCCEEEEEEEEECHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
TMTSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGQFINANDSGVVISSMNNSYWKQRYLGA
HHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCEEECCCCCCEEEECCCCHHHHHHHHHH
KRFF
HCCC
>Mature Secondary Structure
MKKKVAVGLTASAIVGSALAVTPAEAQTIKVKSGDSLWKLAQNYNTSVDAITSANHLKST
CCCEEEEEEEHHHHHCCCEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHH
VISIGQTLNIPNGKSDSGSKKPSSKNSGTTKSVYTVKSGDSLWLIASDFKMSVQELKKLN
HHHHCCEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHC
GLTSDLIRAGQKLQVTGKVSSSTDQKKTTPGSSSNSSKNQSSSSGTYKVKLGDSLWKIAN
CCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHH
SVNMTIAELKVLNNLKTDTIYVNQVLKTKGTSSSGNTPSKDNSNQNKNQTQTSSYTVKSG
CCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
DSLWKIANAYNMTVQQIRSINKLTSDMLRVGQVLKLTKTSSSGSSSSPSTPNQSGDTGKT
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCE
TTYTVKSGDSLWVIAQKFNVTAQQIREKNNLKTDVLQIGQKLTITGQQTNTTPNQSGNTG
EEEEEECCCEEEEEEEECCCHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCC
GTGSTSAKINVMIDAAKAQLGVPYVWGGTTPRGFDCSGFIYYVLNKVTSVSRLSAAGYWN
CCCCCCEEEEEEEEECHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
TMTSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGQFINANDSGVVISSMNNSYWKQRYLGA
HHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCEEECCCCCCEEEECCCCHHHHHHHHHH
KRFF
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 10206711 [H]