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The map label for this gene is yflG [H]

Identifier: 154685243

GI number: 154685243

Start: 778447

End: 779196

Strand: Reverse

Name: yflG [H]

Synonym: RBAM_007890

Alternate gene names: 154685243

Gene position: 779196-778447 (Counterclockwise)

Preceding gene: 154685246

Following gene: 154685242

Centisome position: 19.88

GC content: 48.8

Gene sequence:

>750_bases
ATGATTGTAACGAACGATCAAGAATTAGAAGGCCTTAAAAAAATCGGCAGAATCGTCGCGCTGGCGCGTGAAGAAATGAA
GAAAAAAGCCGAGCCCGGCATGAGCACGAAAGATCTTGACCTGATCGGAAAGGCGGTGCTTGACGAGCACGGAGCCGCTT
CCGCTCCGGAGAAGGAATACGATTTCCCGGGCGTGACATGCATCAGCGTAAATGACGAAGTCGCTCACGGTATTCCGAGC
GCCTCTAAAATCTTAAAAGCCGGAGATCTTGTAAATATCGATATTTCGGCGGAGTTCGGCGGTTTTTATTCCGATACGGG
AATTTCATTTGTACTCGGAGAAGGCGATGAACGTCTGCAAAAGCTTTGTAACTGCGCAGCATCCGCATTTCAGAAAGGAT
TGGATCAGGCAAAAGCCGGCAAACGCCAAAACCAGATCGGCAGAGCCGTCTATCATGAAGCCCGCTCCAACGGATTTACC
GTTATTAAAACGCTGACGGGCCACGGAATCGGCAGAAACCTCCATGAAGCGCCGAACCACATCATGAATTACTACGATCC
GTTTGATAACGCGCTGTTTAAAAACGGAACCGTGATCGCGCTTGAGCCGTTTATTTCCACGAAAGCGGAAACGATTGTTG
AAGCGGGAGACGGCTGGACGTTTAAAACGCCGGATAAAAGCCTTGTCGCCCAAGTGGAACACACCATCGTGATCACAAAA
GACAAACCGATTATTTTAACTGCATTGTAA

Upstream 100 bases:

>100_bases
TTTCCTTCATTTATTCACATAAGGCTTGTTGGTTTTCTATTTATAAACCAGTTGTGGTAAGCTTGAATCAGATGATTAAA
AATAGAAAGCGGGAATGACA

Downstream 100 bases:

>100_bases
TGTTTCATCTCCCTTCCAAACAGGTATACAACAACTAACCCGAAGGAGGGAGACGCATGAAAAAAGATAAAGAAAAAATC
AAAATCGAAAACGAAATACA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP 2 [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIVTNDQELEGLKKIGRIVALAREEMKKKAEPGMSTKDLDLIGKAVLDEHGAASAPEKEYDFPGVTCISVNDEVAHGIPS
ASKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGDERLQKLCNCAASAFQKGLDQAKAGKRQNQIGRAVYHEARSNGFT
VIKTLTGHGIGRNLHEAPNHIMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSLVAQVEHTIVITK
DKPIILTAL

Sequences:

>Translated_249_residues
MIVTNDQELEGLKKIGRIVALAREEMKKKAEPGMSTKDLDLIGKAVLDEHGAASAPEKEYDFPGVTCISVNDEVAHGIPS
ASKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGDERLQKLCNCAASAFQKGLDQAKAGKRQNQIGRAVYHEARSNGFT
VIKTLTGHGIGRNLHEAPNHIMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSLVAQVEHTIVITK
DKPIILTAL
>Mature_249_residues
MIVTNDQELEGLKKIGRIVALAREEMKKKAEPGMSTKDLDLIGKAVLDEHGAASAPEKEYDFPGVTCISVNDEVAHGIPS
ASKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGDERLQKLCNCAASAFQKGLDQAKAGKRQNQIGRAVYHEARSNGFT
VIKTLTGHGIGRNLHEAPNHIMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSLVAQVEHTIVITK
DKPIILTAL

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=246, Percent_Identity=32.1138211382114, Blast_Score=136, Evalue=1e-32,
Organism=Homo sapiens, GI40385867, Length=247, Percent_Identity=30.3643724696356, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1786364, Length=246, Percent_Identity=35.7723577235772, Blast_Score=164, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI71996291, Length=246, Percent_Identity=32.9268292682927, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323273, Length=241, Percent_Identity=31.5352697095436, Blast_Score=125, Evalue=6e-30,
Organism=Drosophila melanogaster, GI21355531, Length=245, Percent_Identity=31.4285714285714, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24583427, Length=240, Percent_Identity=30, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 26927; Mature: 26927

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTNDQELEGLKKIGRIVALAREEMKKKAEPGMSTKDLDLIGKAVLDEHGAASAPEKEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
DFPGVTCISVNDEVAHGIPSASKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGDERLQ
CCCCEEEEECCHHHHHCCCCHHHHHHCCCEEEEEEECCCCCEECCCCCEEEECCCHHHHH
KLCNCAASAFQKGLDQAKAGKRQNQIGRAVYHEARSNGFTVIKTLTGHGIGRNLHEAPNH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
IMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSLVAQVEHTIVITK
HHHHCCCHHHHHHCCCCEEEECCCCCCHHHHHEECCCCCEEECCCHHHHHHHHEEEEEEC
DKPIILTAL
CCCEEEEEC
>Mature Secondary Structure
MIVTNDQELEGLKKIGRIVALAREEMKKKAEPGMSTKDLDLIGKAVLDEHGAASAPEKEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
DFPGVTCISVNDEVAHGIPSASKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGDERLQ
CCCCEEEEECCHHHHHCCCCHHHHHHCCCEEEEEEECCCCCEECCCCCEEEECCCHHHHH
KLCNCAASAFQKGLDQAKAGKRQNQIGRAVYHEARSNGFTVIKTLTGHGIGRNLHEAPNH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
IMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSLVAQVEHTIVITK
HHHHCCCHHHHHHCCCCEEEECCCCCCHHHHHEECCCCCEEECCCHHHHHHHHEEEEEEC
DKPIILTAL
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]