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The map label for this gene is gyrB [H]

Identifier: 154248675

GI number: 154248675

Start: 5281385

End: 5283421

Strand: Direct

Name: gyrB [H]

Synonym: Xaut_4757

Alternate gene names: 154248675

Gene position: 5281385-5283421 (Clockwise)

Preceding gene: 154248670

Following gene: 154248677

Centisome position: 99.48

GC content: 67.75

Gene sequence:

>2037_bases
ATGTCCCAATCCGACGATCTTTTCGGCTCCACCCCGCGTCCGAAGCCCGCCGGCAAGCCGGCCAAGGCGGCAGCTGCCGC
CGTGCCGGCCATGGATGCCGACTACACCGCCGCGCACATTGAGGTGCTCGAAGGACTGGAACCGGTACGTCGCCGGCCCG
GCATGTATATCGGCGGCACCGATTCGAAGGCGCTGCACCACCTCTTCGCCGAGGTGATCGACAATTGCATGGACGAGGCG
GTGGCCGGGCACGCCACCTTCATCGAAGTGCAGATGGCGGCGGACGGCTATCTCACCGTCTCCGACAACGGGCGCGGCAT
TCCGGTGGAGGAACACCCCAAGTTCCCCGGCAAGTCGGCCCTCGAAGTGATCATGACCACCCTGCACGCCGGCGGAAAGT
TCGACAGCAAGGTCTACGAGACGTCCGGCGGCCTCCACGGCGTCGGCATCTCGGTGGTGAACGCGCTGTCCGACGACCTC
GAGGTGGAGGTGACGCGCAACCAGACCCTCTACCGCCAGAGCTTCTCCAAGGGCGTGCCCCAGGGCAAGCTGAAGGAAGT
CGGCCGCATCACCAACCGGCGCGGCACCCGTGTGCGCTTCCATCCGGACCCCGAGATCTTCGGCCCCTCCGCCCGCTTCG
AGCCGGCCCGGGTGTTCCGCATGGCCCGCTCCAAGGCCTATCTGTTCGGCGGCGTGGAAATCCGCTGGTCCTGCGACCCC
GAGCTGCTGAAGGGCATCGAGGGGGTTCCGGAGAAGACCAGCTTCCGCTTCCCCGGCGGCCTGCGCGACTATCTCGCCGC
CGACCTCGAAGGCCAGACCCTGGTGCATTCGGACATCTTCTCCGGCCGTATCCAGAAGCCGGGCGGCCATGGCTCGGTGG
AATGGGCGGTGGCATGGGTGGCGGACGCGGACGGCTCCATCTCCTCCTACTGCAACACCATCCCCACCCCCGACGGCGGC
ACCCATGAGAACGGCCTGCGGGCCGTGCTGCTGAAGGGGCTGAAGGATCATGCCGAGCGCTCCGGCCAGGGCAAGCGCGG
CTCGGTCATCACCGGCGACGATGTCACCACCGGCTGCGCCGCCATGCTCTCCGTGTTCGTGCGCGAGCCGGAATTCCAGG
GCCAGACCAAGGACCGCCTCGCCACTGCCGAGGCCGCCCGCATCGTGGAACAGGGCCTGCGCGACCCGTTCGACCACTGG
CTCGCTGCCAATCCGGTGCAGGCCAGCCGGCTGCTCGAGCACGTCATCGACCGCGCCGAGGAGCGCCTGCGCCGCAAGCA
GGAAAAGGAAGTCTCTCGCAAGACCGCCGTGCGCAAGCTGCGCCTGCCCGGCAAGCTGGCCGACTGCTCCAACACCGAGG
CGGCCGGCTCCGAGATCTTCATCGTGGAGGGCGACTCGGCCGGCGGCTCGGCCAAGCAGGCACGCGACCGCAAGACCCAG
GCCGTGCTGCCCCTGCGCGGCAAGATCCTGAACGTCGCCAGCGCCACCCGGGACAAGCTCGCCCAGAACCAGCAGCTCGC
CGACCTCGGCCAGGCGCTGGGCTGTGGCATGCTCTCCCATTATCGCGACGCGGATCTGCGGTACGAGAAGGTCATCATCA
TGACCGACGCCGACGTGGACGGCGCGCACATCGCCTCGCTGCTGGTCACCTTCTTCTTCCGGCAGATGCCGAAGCTGATC
GAGGACGGCCACCTCTTCCTCGCCGTGCCGCCGCTCTACCGCATCACCCAGGGCGCCAAGACGCTCTATGCGCGGGACGA
CGCCCACAAGGAAAAGCTGCTGAAGAAGGAATTCACCGGCAAGGGCAAGGTGGAGGTGGGCCGCTTCAAGGGTCTCGGCG
AGATGATGCCGGCTCAGCTGAAGGAAACCACCATGGATCCGCGCACGCGCACGCTCCAGCGTGTCACCATCGAGGATGCC
GAGCAGGCGGCCACCACGGACCTCGTGGAACGCCTCATGGGCAACAGGCCCGAGGCGCGCTTCGCCTTTATCTCCGAGCG
CGCCGCCTTCGCCGGCGAGGCCTTGCTCGACATCTGA

Upstream 100 bases:

>100_bases
TCTGTTCCCATCGGCGCCGGCCTCTGCCATGCTGTGAACAGCGACGCCTTCTCCCTGCGCGCGATTCGCGCAGGACCGGC
CCTTCTGCTATCGAGCGCCC

Downstream 100 bases:

>100_bases
CCCCCATAGCCTTTGAGCTTGCCTCAAGGGCTATGGGTCAAGCCCGCGCGGCGCTTGAGCGAAGCCAATTGAAGACGCCC
CCTCAGCCGGTCGTCACCTT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MSQSDDLFGSTPRPKPAGKPAKAAAAAVPAMDADYTAAHIEVLEGLEPVRRRPGMYIGGTDSKALHHLFAEVIDNCMDEA
VAGHATFIEVQMAADGYLTVSDNGRGIPVEEHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGISVVNALSDDL
EVEVTRNQTLYRQSFSKGVPQGKLKEVGRITNRRGTRVRFHPDPEIFGPSARFEPARVFRMARSKAYLFGGVEIRWSCDP
ELLKGIEGVPEKTSFRFPGGLRDYLAADLEGQTLVHSDIFSGRIQKPGGHGSVEWAVAWVADADGSISSYCNTIPTPDGG
THENGLRAVLLKGLKDHAERSGQGKRGSVITGDDVTTGCAAMLSVFVREPEFQGQTKDRLATAEAARIVEQGLRDPFDHW
LAANPVQASRLLEHVIDRAEERLRRKQEKEVSRKTAVRKLRLPGKLADCSNTEAAGSEIFIVEGDSAGGSAKQARDRKTQ
AVLPLRGKILNVASATRDKLAQNQQLADLGQALGCGMLSHYRDADLRYEKVIIMTDADVDGAHIASLLVTFFFRQMPKLI
EDGHLFLAVPPLYRITQGAKTLYARDDAHKEKLLKKEFTGKGKVEVGRFKGLGEMMPAQLKETTMDPRTRTLQRVTIEDA
EQAATTDLVERLMGNRPEARFAFISERAAFAGEALLDI

Sequences:

>Translated_678_residues
MSQSDDLFGSTPRPKPAGKPAKAAAAAVPAMDADYTAAHIEVLEGLEPVRRRPGMYIGGTDSKALHHLFAEVIDNCMDEA
VAGHATFIEVQMAADGYLTVSDNGRGIPVEEHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGISVVNALSDDL
EVEVTRNQTLYRQSFSKGVPQGKLKEVGRITNRRGTRVRFHPDPEIFGPSARFEPARVFRMARSKAYLFGGVEIRWSCDP
ELLKGIEGVPEKTSFRFPGGLRDYLAADLEGQTLVHSDIFSGRIQKPGGHGSVEWAVAWVADADGSISSYCNTIPTPDGG
THENGLRAVLLKGLKDHAERSGQGKRGSVITGDDVTTGCAAMLSVFVREPEFQGQTKDRLATAEAARIVEQGLRDPFDHW
LAANPVQASRLLEHVIDRAEERLRRKQEKEVSRKTAVRKLRLPGKLADCSNTEAAGSEIFIVEGDSAGGSAKQARDRKTQ
AVLPLRGKILNVASATRDKLAQNQQLADLGQALGCGMLSHYRDADLRYEKVIIMTDADVDGAHIASLLVTFFFRQMPKLI
EDGHLFLAVPPLYRITQGAKTLYARDDAHKEKLLKKEFTGKGKVEVGRFKGLGEMMPAQLKETTMDPRTRTLQRVTIEDA
EQAATTDLVERLMGNRPEARFAFISERAAFAGEALLDI
>Mature_677_residues
SQSDDLFGSTPRPKPAGKPAKAAAAAVPAMDADYTAAHIEVLEGLEPVRRRPGMYIGGTDSKALHHLFAEVIDNCMDEAV
AGHATFIEVQMAADGYLTVSDNGRGIPVEEHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGISVVNALSDDLE
VEVTRNQTLYRQSFSKGVPQGKLKEVGRITNRRGTRVRFHPDPEIFGPSARFEPARVFRMARSKAYLFGGVEIRWSCDPE
LLKGIEGVPEKTSFRFPGGLRDYLAADLEGQTLVHSDIFSGRIQKPGGHGSVEWAVAWVADADGSISSYCNTIPTPDGGT
HENGLRAVLLKGLKDHAERSGQGKRGSVITGDDVTTGCAAMLSVFVREPEFQGQTKDRLATAEAARIVEQGLRDPFDHWL
AANPVQASRLLEHVIDRAEERLRRKQEKEVSRKTAVRKLRLPGKLADCSNTEAAGSEIFIVEGDSAGGSAKQARDRKTQA
VLPLRGKILNVASATRDKLAQNQQLADLGQALGCGMLSHYRDADLRYEKVIIMTDADVDGAHIASLLVTFFFRQMPKLIE
DGHLFLAVPPLYRITQGAKTLYARDDAHKEKLLKKEFTGKGKVEVGRFKGLGEMMPAQLKETTMDPRTRTLQRVTIEDAE
QAATTDLVERLMGNRPEARFAFISERAAFAGEALLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=670, Percent_Identity=24.0298507462687, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI19913406, Length=623, Percent_Identity=23.5955056179775, Blast_Score=127, Evalue=5e-29,
Organism=Escherichia coli, GI1789408, Length=645, Percent_Identity=43.7209302325581, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI48994957, Length=561, Percent_Identity=44.7415329768271, Blast_Score=451, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI212645845, Length=573, Percent_Identity=24.4328097731239, Blast_Score=122, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17535065, Length=587, Percent_Identity=22.6575809199319, Blast_Score=107, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324241, Length=651, Percent_Identity=23.8095238095238, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136538, Length=587, Percent_Identity=26.4054514480409, Blast_Score=120, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74004; Mature: 73873

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSDDLFGSTPRPKPAGKPAKAAAAAVPAMDADYTAAHIEVLEGLEPVRRRPGMYIGGT
CCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCC
DSKALHHLFAEVIDNCMDEAVAGHATFIEVQMAADGYLTVSDNGRGIPVEEHPKFPGKSA
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHH
LEVIMTTLHAGGKFDSKVYETSGGLHGVGISVVNALSDDLEVEVTRNQTLYRQSFSKGVP
HHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCC
QGKLKEVGRITNRRGTRVRFHPDPEIFGPSARFEPARVFRMARSKAYLFGGVEIRWSCDP
CHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEEECEEEEECCCH
ELLKGIEGVPEKTSFRFPGGLRDYLAADLEGQTLVHSDIFSGRIQKPGGHGSVEWAVAWV
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEHHHHHCCCCCCCCCCCCEEEEEEEE
ADADGSISSYCNTIPTPDGGTHENGLRAVLLKGLKDHAERSGQGKRGSVITGDDVTTGCA
ECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHH
AMLSVFVREPEFQGQTKDRLATAEAARIVEQGLRDPFDHWLAANPVQASRLLEHVIDRAE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHH
ERLRRKQEKEVSRKTAVRKLRLPGKLADCSNTEAAGSEIFIVEGDSAGGSAKQARDRKTQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
AVLPLRGKILNVASATRDKLAQNQQLADLGQALGCGMLSHYRDADLRYEKVIIMTDADVD
EECCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCC
GAHIASLLVTFFFRQMPKLIEDGHLFLAVPPLYRITQGAKTLYARDDAHKEKLLKKEFTG
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCHHEEECCHHHHHHHHHHHCCC
KGKVEVGRFKGLGEMMPAQLKETTMDPRTRTLQRVTIEDAEQAATTDLVERLMGNRPEAR
CCCEEEHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FAFISERAAFAGEALLDI
HHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
SQSDDLFGSTPRPKPAGKPAKAAAAAVPAMDADYTAAHIEVLEGLEPVRRRPGMYIGGT
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCC
DSKALHHLFAEVIDNCMDEAVAGHATFIEVQMAADGYLTVSDNGRGIPVEEHPKFPGKSA
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHH
LEVIMTTLHAGGKFDSKVYETSGGLHGVGISVVNALSDDLEVEVTRNQTLYRQSFSKGVP
HHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCC
QGKLKEVGRITNRRGTRVRFHPDPEIFGPSARFEPARVFRMARSKAYLFGGVEIRWSCDP
CHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEEECEEEEECCCH
ELLKGIEGVPEKTSFRFPGGLRDYLAADLEGQTLVHSDIFSGRIQKPGGHGSVEWAVAWV
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEHHHHHCCCCCCCCCCCCEEEEEEEE
ADADGSISSYCNTIPTPDGGTHENGLRAVLLKGLKDHAERSGQGKRGSVITGDDVTTGCA
ECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHH
AMLSVFVREPEFQGQTKDRLATAEAARIVEQGLRDPFDHWLAANPVQASRLLEHVIDRAE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHH
ERLRRKQEKEVSRKTAVRKLRLPGKLADCSNTEAAGSEIFIVEGDSAGGSAKQARDRKTQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
AVLPLRGKILNVASATRDKLAQNQQLADLGQALGCGMLSHYRDADLRYEKVIIMTDADVD
EECCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCC
GAHIASLLVTFFFRQMPKLIEDGHLFLAVPPLYRITQGAKTLYARDDAHKEKLLKKEFTG
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCHHEEECCHHHHHHHHHHHCCC
KGKVEVGRFKGLGEMMPAQLKETTMDPRTRTLQRVTIEDAEQAATTDLVERLMGNRPEAR
CCCEEEHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FAFISERAAFAGEALLDI
HHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]