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The map label for this gene is mutB [H]

Identifier: 154248649

GI number: 154248649

Start: 5249604

End: 5251760

Strand: Direct

Name: mutB [H]

Synonym: Xaut_4731

Alternate gene names: 154248649

Gene position: 5249604-5251760 (Clockwise)

Preceding gene: 154248648

Following gene: 154248650

Centisome position: 98.88

GC content: 68.1

Gene sequence:

>2157_bases
ATGAGCCGGATCCCCAACTTCGCCGACATCGACTGGCGCGCGCCGGAGCTGGTCGCCCCCTCCGCGGCCGCCGCCCCCTG
GACCACGCCCGAGGGCATCCCGGTGAAGCCGCTCTATGGGCCGGCGGACATCGAGGGCCTCGGCTACATCGATACCTATC
CCGGTATCGCGCCATTCCTGCGCGGGCCTTATGCCCCCATGTATGCGACCCAGCCCTGGACCATCCGGCAATATGCCGGC
TTCTCCACGGCGGAGGATTCCAACGCCTTCTACCGCCGCAACCTCGCGGCCGGGCAGAAGGGCCTGTCGGTCGCTTTCGA
CCTCGCCACCCATCGCGGCTATGACAGCGACCATCCGCGCGTCGCCGGCGACGTGGGCATGGCCGGCGTCGCCATCGACA
GCATCTACGACATGCGCACCCTGTTCTCCGGCATCCCGCTGGACCAGATGAGCGTGTCGATGACCATGAATGGGGCCGTT
CTTCCCATTCTGGCGCTCTATGTGGTGGCGGCGGAAGAGCAGGGCGTGGCTCCGGCCAAGCTTTCCGGCACCATCCAGAA
CGACATTCTCAAAGAATTCATGGTGCGGAACACCTATATCTATCCGCCCACCCCCTCCATGCGCATCATCTCGGACATCT
TCGCCTTCACGTCGAAGGAGATGCCGCGCTTCAACTCCATCTCCATCTCCGGCTACCACATGCAGGAAGCCGGCGCGACG
CAGGACCTGGAGCTGGCCTACACGCTGGCGGATGGCGTCGAATATGGCCGCGCCGGCGCCGCCGCCGGCCTGCCCATCGA
CGTGTTCGCGCCGCGCCTGTCGTTCTTCTGGGCCATCGGCATGAACTTCTACATGGAGGTGGCGAAGCTGCGCGCCGCCC
GCCTCCTGTGGACGCGTCTGATGACCGACCTTGGCGCCAAGAACCCCAAGTCCCTGCCCCTGCGCACCCATTCGCAGACC
TCCGGCTGGTCGCTGACGGCGCAGGACGTGTTCAACAACGTCATGCGCACCGCCATCGAGGCCATGGCCGCGACTCAGGG
CCAGACCCAGTCGCTGCACACCAATGCGCTGGACGAGGCGCTGGCCCTGCCGACGGATTTCTCCGCCCGCATCGCCCGCA
ACACCCAGATCCTGCTCCAGCAGGAGAGCGGCACCACCCGCCAGATCGACCTGTGGGGCGGCTCCTATTTCGTGGAGAAG
CTCACCGCTGATCTCGCCGCCAAGGCCTGGGCGCACATCCAGGAGGTGGAGGCGCTGGGCGGCATGGCCAAGGCCATCGA
GGCCGGCATCCCGAAGCTCCGCATCGAGGAGGCCGCCGCCACCACCCAAGCCCGCATCGATGGCGGGGCGCAGACCGTGG
TGGGCGTCAACAAGTTCAAGCCCGAGCGCGACCGCATGATCGACGTGCTGAAGGTGGAGAACGCCACCGTGCGCGCCGCC
CAGATCGACAAGCTCAAGCGCCTGCGCGCCGAGCGCGACCAGGCCGAGGTGGACGGCAAGCTCGAAGCCCTCACCAACGG
CGCGGCGGGCAACGGCAACCTGCTGGCGCTGGCCATTGACGCGGCCCGGGCCAAGGCCACGGTGGGCGAGATTTCCGATG
CGCTGGAGAAGGTGTTCGGCCGCCATCGCGCCGAGATCCGCGCCATCTCCGGCGTCTACAAGCGCGAGGCGACCGCCATG
ACGGACAAGGTGGGCAAGCTGCAGAAGCTGGTGGAAGCATTCGAGCAGGCGGAGGGCCGCCGGCCGCGCATCCTCGTCGC
CAAGGTGGGCCAGGACGGCCACGACCGCGGCCAGAAGGTGATCGCCTCCGCCTTCGCAGACCTCGGCTTCGACGTGGACA
TCGGCCCCCTCTTCGCCACCCCGGAGGAAGCCGCACGGCAGGCGGTGGAGAACGACGTGCACGTGGTCGGCATCTCTTCG
CTGGCCGCCGGCCATCTCACCCTGGTGCCGGCGCTGAAGGCGGCGCTGGAAGCGGAGGGCCGGGGCGACATCATGGTGGT
GGTCGGCGGCGTGGTGCCCCCGCAGGACTATGACGCCCTGAAGGCCGCCGGCGCCGAGGCCATCTTTCCCCCCGGCACGG
TCATCGCCGATGCCGCGAAGGAACTGCTCGCCACCCTCTCCCACCGCCTCGGGCACAATCTCGAAGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
CATCCCAAGTGCTCCGGCTCCTGGTTTGCCAAGCGGGCCTGAGCCCGAAACCTGAGCCTTTTCACCAATGCCACCGCGGC
CGATTTCGAGGGAACAGAAG

Downstream 100 bases:

>100_bases
GTTCAGCCTCCGGGCTGGCTCCGCTGGGCTCCACCCTGCGGGTGCTGGAGCCGGCCCCGCATGTGCTCGCCTTCTATGAC
GGGCGGGTGCCGGGAGTGCG

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MSRIPNFADIDWRAPELVAPSAAAAPWTTPEGIPVKPLYGPADIEGLGYIDTYPGIAPFLRGPYAPMYATQPWTIRQYAG
FSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFSGIPLDQMSVSMTMNGAV
LPILALYVVAAEEQGVAPAKLSGTIQNDILKEFMVRNTYIYPPTPSMRIISDIFAFTSKEMPRFNSISISGYHMQEAGAT
QDLELAYTLADGVEYGRAGAAAGLPIDVFAPRLSFFWAIGMNFYMEVAKLRAARLLWTRLMTDLGAKNPKSLPLRTHSQT
SGWSLTAQDVFNNVMRTAIEAMAATQGQTQSLHTNALDEALALPTDFSARIARNTQILLQQESGTTRQIDLWGGSYFVEK
LTADLAAKAWAHIQEVEALGGMAKAIEAGIPKLRIEEAAATTQARIDGGAQTVVGVNKFKPERDRMIDVLKVENATVRAA
QIDKLKRLRAERDQAEVDGKLEALTNGAAGNGNLLALAIDAARAKATVGEISDALEKVFGRHRAEIRAISGVYKREATAM
TDKVGKLQKLVEAFEQAEGRRPRILVAKVGQDGHDRGQKVIASAFADLGFDVDIGPLFATPEEAARQAVENDVHVVGISS
LAAGHLTLVPALKAALEAEGRGDIMVVVGGVVPPQDYDALKAAGAEAIFPPGTVIADAAKELLATLSHRLGHNLEAAE

Sequences:

>Translated_718_residues
MSRIPNFADIDWRAPELVAPSAAAAPWTTPEGIPVKPLYGPADIEGLGYIDTYPGIAPFLRGPYAPMYATQPWTIRQYAG
FSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFSGIPLDQMSVSMTMNGAV
LPILALYVVAAEEQGVAPAKLSGTIQNDILKEFMVRNTYIYPPTPSMRIISDIFAFTSKEMPRFNSISISGYHMQEAGAT
QDLELAYTLADGVEYGRAGAAAGLPIDVFAPRLSFFWAIGMNFYMEVAKLRAARLLWTRLMTDLGAKNPKSLPLRTHSQT
SGWSLTAQDVFNNVMRTAIEAMAATQGQTQSLHTNALDEALALPTDFSARIARNTQILLQQESGTTRQIDLWGGSYFVEK
LTADLAAKAWAHIQEVEALGGMAKAIEAGIPKLRIEEAAATTQARIDGGAQTVVGVNKFKPERDRMIDVLKVENATVRAA
QIDKLKRLRAERDQAEVDGKLEALTNGAAGNGNLLALAIDAARAKATVGEISDALEKVFGRHRAEIRAISGVYKREATAM
TDKVGKLQKLVEAFEQAEGRRPRILVAKVGQDGHDRGQKVIASAFADLGFDVDIGPLFATPEEAARQAVENDVHVVGISS
LAAGHLTLVPALKAALEAEGRGDIMVVVGGVVPPQDYDALKAAGAEAIFPPGTVIADAAKELLATLSHRLGHNLEAAE
>Mature_717_residues
SRIPNFADIDWRAPELVAPSAAAAPWTTPEGIPVKPLYGPADIEGLGYIDTYPGIAPFLRGPYAPMYATQPWTIRQYAGF
STAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFSGIPLDQMSVSMTMNGAVL
PILALYVVAAEEQGVAPAKLSGTIQNDILKEFMVRNTYIYPPTPSMRIISDIFAFTSKEMPRFNSISISGYHMQEAGATQ
DLELAYTLADGVEYGRAGAAAGLPIDVFAPRLSFFWAIGMNFYMEVAKLRAARLLWTRLMTDLGAKNPKSLPLRTHSQTS
GWSLTAQDVFNNVMRTAIEAMAATQGQTQSLHTNALDEALALPTDFSARIARNTQILLQQESGTTRQIDLWGGSYFVEKL
TADLAAKAWAHIQEVEALGGMAKAIEAGIPKLRIEEAAATTQARIDGGAQTVVGVNKFKPERDRMIDVLKVENATVRAAQ
IDKLKRLRAERDQAEVDGKLEALTNGAAGNGNLLALAIDAARAKATVGEISDALEKVFGRHRAEIRAISGVYKREATAMT
DKVGKLQKLVEAFEQAEGRRPRILVAKVGQDGHDRGQKVIASAFADLGFDVDIGPLFATPEEAARQAVENDVHVVGISSL
AAGHLTLVPALKAALEAEGRGDIMVVVGGVVPPQDYDALKAAGAEAIFPPGTVIADAAKELLATLSHRLGHNLEAAE

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=686, Percent_Identity=63.265306122449, Blast_Score=885, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=684, Percent_Identity=62.5730994152047, Blast_Score=847, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=686, Percent_Identity=63.265306122449, Blast_Score=890, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 77124; Mature: 76993

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIPNFADIDWRAPELVAPSAAAAPWTTPEGIPVKPLYGPADIEGLGYIDTYPGIAPFL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RGPYAPMYATQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPR
CCCCCCCCCCCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCC
VAGDVGMAGVAIDSIYDMRTLFSGIPLDQMSVSMTMNGAVLPILALYVVAAEEQGVAPAK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCHH
LSGTIQNDILKEFMVRNTYIYPPTPSMRIISDIFAFTSKEMPRFNSISISGYHMQEAGAT
HCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECEEECCCCCC
QDLELAYTLADGVEYGRAGAAAGLPIDVFAPRLSFFWAIGMNFYMEVAKLRAARLLWTRL
CCCEEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTDLGAKNPKSLPLRTHSQTSGWSLTAQDVFNNVMRTAIEAMAATQGQTQSLHTNALDEA
HHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LALPTDFSARIARNTQILLQQESGTTRQIDLWGGSYFVEKLTADLAAKAWAHIQEVEALG
HCCCCCCHHHHHCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMAKAIEAGIPKLRIEEAAATTQARIDGGAQTVVGVNKFKPERDRMIDVLKVENATVRAA
HHHHHHHHCCCCEEEHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHEEHEEECCCEEHHH
QIDKLKRLRAERDQAEVDGKLEALTNGAAGNGNLLALAIDAARAKATVGEISDALEKVFG
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHH
RHRAEIRAISGVYKREATAMTDKVGKLQKLVEAFEQAEGRRPRILVAKVGQDGHDRGQKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
IASAFADLGFDVDIGPLFATPEEAARQAVENDVHVVGISSLAAGHLTLVPALKAALEAEG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEHHHCCCHHHHHHHHHHHHHCCC
RGDIMVVVGGVVPPQDYDALKAAGAEAIFPPGTVIADAAKELLATLSHRLGHNLEAAE
CCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SRIPNFADIDWRAPELVAPSAAAAPWTTPEGIPVKPLYGPADIEGLGYIDTYPGIAPFL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RGPYAPMYATQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPR
CCCCCCCCCCCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCC
VAGDVGMAGVAIDSIYDMRTLFSGIPLDQMSVSMTMNGAVLPILALYVVAAEEQGVAPAK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCHH
LSGTIQNDILKEFMVRNTYIYPPTPSMRIISDIFAFTSKEMPRFNSISISGYHMQEAGAT
HCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECEEECCCCCC
QDLELAYTLADGVEYGRAGAAAGLPIDVFAPRLSFFWAIGMNFYMEVAKLRAARLLWTRL
CCCEEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTDLGAKNPKSLPLRTHSQTSGWSLTAQDVFNNVMRTAIEAMAATQGQTQSLHTNALDEA
HHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LALPTDFSARIARNTQILLQQESGTTRQIDLWGGSYFVEKLTADLAAKAWAHIQEVEALG
HCCCCCCHHHHHCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMAKAIEAGIPKLRIEEAAATTQARIDGGAQTVVGVNKFKPERDRMIDVLKVENATVRAA
HHHHHHHHCCCCEEEHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHEEHEEECCCEEHHH
QIDKLKRLRAERDQAEVDGKLEALTNGAAGNGNLLALAIDAARAKATVGEISDALEKVFG
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHH
RHRAEIRAISGVYKREATAMTDKVGKLQKLVEAFEQAEGRRPRILVAKVGQDGHDRGQKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
IASAFADLGFDVDIGPLFATPEEAARQAVENDVHVVGISSLAAGHLTLVPALKAALEAEG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEHHHCCCHHHHHHHHHHHHHCCC
RGDIMVVVGGVVPPQDYDALKAAGAEAIFPPGTVIADAAKELLATLSHRLGHNLEAAE
CCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]