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The map label for this gene is gsiB [H]

Identifier: 154248579

GI number: 154248579

Start: 5150891

End: 5152513

Strand: Reverse

Name: gsiB [H]

Synonym: Xaut_4660

Alternate gene names: 154248579

Gene position: 5152513-5150891 (Counterclockwise)

Preceding gene: 154248580

Following gene: 154248578

Centisome position: 97.05

GC content: 63.77

Gene sequence:

>1623_bases
ATGAACCGCCGCGAATTCGTGAAGTCTGCGTCTGCCACCGCCGTTGCCACCGGAACCGGTGTCGCCGCCCCGGCGGTCTT
CTCCTCGGCACAGGCGCAGGCGCGCAACGAGACGCTGCTGATCGTCTCCGAGAGCGGCCCCAACAACCTCGACATCCATG
GTGTCGGCACCAACGTGCCGGGCTATGAGGCGAGCTGGAACACCTACGACCGCCTCATCACCCACGAGATGACCGAGAAG
GACGGGGTCCGCTACTACGACCGCGACAAGCTGAAGGGCGAGCTCGCCGAGGACATGAACATCGGCGACATGTCGGTGAC
CTTCAAGCTGAAGAAGAACGCCACCTTCCAGGACGGCACCCCGGTCACCGCCAAGGACGTGAAGTGGTCGCTGGACCGCG
CCGTCTCCGTGGGCGGCTTCCCCACCTTCCAGATGAAGGCCGGCTCGCTGGAGAAGCCCGAGCAGTTCGTGGTGGTGGAT
GACCACACGGTGCGCGTGGACTTCATCCGCAAGGACCGCCTCACCATCCCCGATCTCGCCGTGATCGTGCCCTGCGTCAT
CAATTCCGGGCTGGTGCAGAAGAACGCCACCGAAAAGGACCCCTGGGGCCTCGAATACACCAAGCAGAACACCGCCGGCT
CCGGCGCCTATCGCGTCACCAAGTGGACCCCCGGCACCGAGGTGATCTTCGAGCGCTTCGAGGACTGGAAGGGCGGCCCG
CTGCCCAAGATCAAGCGCGTGATCTGGCGCATGGTGCCCTCCGCCGGCAACCGCCGGGCGCTGCTGGAGCGCGGCGACGC
CGACATCTCCTACGACCTGCCCAACAAGGATTTCGTGGAGCTGAAGCAGGCCGGCAAGCTGAACATCACGTCGGTGCCCT
ATTCCAACGGTGTCCAGTACATCGGCATGAACGTGAAGAACCCGCCCTTCGACAATCTGAAGGTGCGCCAGGCCATCGCT
TACGCCATCCCCTACCAGAAGATCATGGACGCCGCCCTGTTCGGCCTCGCCAAGCCCATGTTCGGCGCCCCGGCGGATGC
GCAGACCCAGGTCAAGTGGCCGCAGCCCACCAAGTTCGTCACCGACCTCGCCAAGGCCAAGCAATTGCTGGCGGAGGCGG
GCTATCCCGACGGGCTGGAGACGACGCTGTCCTTCGACCTCGGCTTTGCCGGCGTGAACGAGCCGCTGTGCGTGCTGCTG
CAGGAAAACCTGGCGCAGATCGGCATCAAGACCACCATCAACAAGATCCCCGGCGCCAACTGGCGCACCGAGCTGACGAA
GAAGGTGCTGCCGCTGTTCACCAACGTGTTCTCGGGTTGGCTGGACTATCCCGAATACTTCTTCTTCTGGTGCTACCACG
GCAACAATTCGATCTTCAACACCATGAGCTACCAGTCGGCGGCCATGGATGCCTTCATCGACGGCGCCCGTGCCGCCGCC
GCCAACGGCGACAAGGCGGCCTATGATGCGGACGTGAAGGGCATGGTGGACCTCGCCTTCGCCGACGTGCCGCGTATCCC
GCTCTACCAGCCCTATGTGAACGTGGCGATGCAGAAGAACATCACCGGCTACGAATACTGGTTCCACCGCCGTCTCGACT
ATCGCGCTTTCCAGAAGGGGTGA

Upstream 100 bases:

>100_bases
GACCATATTATTGATATTAAATGTAAATCATATTTTTGTATGCAATTGGCATGCAGCTTGAATGGGAATGGGCGACAGTC
ACCATTGGGGATGCCGTCCG

Downstream 100 bases:

>100_bases
GGGTTCCACGCACCCCGGGCCGGCGCGACCGGACCGGTGCGTGTGGTCGGGTTTTCAACCGCCGTCATCCCCCGGCTCGA
CCGGGGGATCCACGGAGCGG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MNRREFVKSASATAVATGTGVAAPAVFSSAQAQARNETLLIVSESGPNNLDIHGVGTNVPGYEASWNTYDRLITHEMTEK
DGVRYYDRDKLKGELAEDMNIGDMSVTFKLKKNATFQDGTPVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVD
DHTVRVDFIRKDRLTIPDLAVIVPCVINSGLVQKNATEKDPWGLEYTKQNTAGSGAYRVTKWTPGTEVIFERFEDWKGGP
LPKIKRVIWRMVPSAGNRRALLERGDADISYDLPNKDFVELKQAGKLNITSVPYSNGVQYIGMNVKNPPFDNLKVRQAIA
YAIPYQKIMDAALFGLAKPMFGAPADAQTQVKWPQPTKFVTDLAKAKQLLAEAGYPDGLETTLSFDLGFAGVNEPLCVLL
QENLAQIGIKTTINKIPGANWRTELTKKVLPLFTNVFSGWLDYPEYFFFWCYHGNNSIFNTMSYQSAAMDAFIDGARAAA
ANGDKAAYDADVKGMVDLAFADVPRIPLYQPYVNVAMQKNITGYEYWFHRRLDYRAFQKG

Sequences:

>Translated_540_residues
MNRREFVKSASATAVATGTGVAAPAVFSSAQAQARNETLLIVSESGPNNLDIHGVGTNVPGYEASWNTYDRLITHEMTEK
DGVRYYDRDKLKGELAEDMNIGDMSVTFKLKKNATFQDGTPVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVD
DHTVRVDFIRKDRLTIPDLAVIVPCVINSGLVQKNATEKDPWGLEYTKQNTAGSGAYRVTKWTPGTEVIFERFEDWKGGP
LPKIKRVIWRMVPSAGNRRALLERGDADISYDLPNKDFVELKQAGKLNITSVPYSNGVQYIGMNVKNPPFDNLKVRQAIA
YAIPYQKIMDAALFGLAKPMFGAPADAQTQVKWPQPTKFVTDLAKAKQLLAEAGYPDGLETTLSFDLGFAGVNEPLCVLL
QENLAQIGIKTTINKIPGANWRTELTKKVLPLFTNVFSGWLDYPEYFFFWCYHGNNSIFNTMSYQSAAMDAFIDGARAAA
ANGDKAAYDADVKGMVDLAFADVPRIPLYQPYVNVAMQKNITGYEYWFHRRLDYRAFQKG
>Mature_540_residues
MNRREFVKSASATAVATGTGVAAPAVFSSAQAQARNETLLIVSESGPNNLDIHGVGTNVPGYEASWNTYDRLITHEMTEK
DGVRYYDRDKLKGELAEDMNIGDMSVTFKLKKNATFQDGTPVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVD
DHTVRVDFIRKDRLTIPDLAVIVPCVINSGLVQKNATEKDPWGLEYTKQNTAGSGAYRVTKWTPGTEVIFERFEDWKGGP
LPKIKRVIWRMVPSAGNRRALLERGDADISYDLPNKDFVELKQAGKLNITSVPYSNGVQYIGMNVKNPPFDNLKVRQAIA
YAIPYQKIMDAALFGLAKPMFGAPADAQTQVKWPQPTKFVTDLAKAKQLLAEAGYPDGLETTLSFDLGFAGVNEPLCVLL
QENLAQIGIKTTINKIPGANWRTELTKKVLPLFTNVFSGWLDYPEYFFFWCYHGNNSIFNTMSYQSAAMDAFIDGARAAA
ANGDKAAYDADVKGMVDLAFADVPRIPLYQPYVNVAMQKNITGYEYWFHRRLDYRAFQKG

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=451, Percent_Identity=29.9334811529933, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1787762, Length=508, Percent_Identity=26.3779527559055, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789966, Length=454, Percent_Identity=25.5506607929515, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787495, Length=549, Percent_Identity=24.0437158469945, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60056; Mature: 60056

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRREFVKSASATAVATGTGVAAPAVFSSAQAQARNETLLIVSESGPNNLDIHGVGTNVP
CCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCC
GYEASWNTYDRLITHEMTEKDGVRYYDRDKLKGELAEDMNIGDMSVTFKLKKNATFQDGT
CCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
PVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVDDHTVRVDFIRKDRLTIPDLA
CCCHHHCHHHHHHHEECCCCCEEEECCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHH
VIVPCVINSGLVQKNATEKDPWGLEYTKQNTAGSGAYRVTKWTPGTEVIFERFEDWKGGP
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCC
LPKIKRVIWRMVPSAGNRRALLERGDADISYDLPNKDFVELKQAGKLNITSVPYSNGVQY
CHHHHHHHHHHCCCCCCCCHHHCCCCCCEEECCCCCHHHHHHHCCCEEEEECCCCCCCEE
IGMNVKNPPFDNLKVRQAIAYAIPYQKIMDAALFGLAKPMFGAPADAQTQVKWPQPTKFV
EEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCHHHH
TDLAKAKQLLAEAGYPDGLETTLSFDLGFAGVNEPLCVLLQENLAQIGIKTTINKIPGAN
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
WRTELTKKVLPLFTNVFSGWLDYPEYFFFWCYHGNNSIFNTMSYQSAAMDAFIDGARAAA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHCCHHHHC
ANGDKAAYDADVKGMVDLAFADVPRIPLYQPYVNVAMQKNITGYEYWFHRRLDYRAFQKG
CCCCCCEECCCCCCCEEEHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MNRREFVKSASATAVATGTGVAAPAVFSSAQAQARNETLLIVSESGPNNLDIHGVGTNVP
CCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCC
GYEASWNTYDRLITHEMTEKDGVRYYDRDKLKGELAEDMNIGDMSVTFKLKKNATFQDGT
CCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
PVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVDDHTVRVDFIRKDRLTIPDLA
CCCHHHCHHHHHHHEECCCCCEEEECCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHH
VIVPCVINSGLVQKNATEKDPWGLEYTKQNTAGSGAYRVTKWTPGTEVIFERFEDWKGGP
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCC
LPKIKRVIWRMVPSAGNRRALLERGDADISYDLPNKDFVELKQAGKLNITSVPYSNGVQY
CHHHHHHHHHHCCCCCCCCHHHCCCCCCEEECCCCCHHHHHHHCCCEEEEECCCCCCCEE
IGMNVKNPPFDNLKVRQAIAYAIPYQKIMDAALFGLAKPMFGAPADAQTQVKWPQPTKFV
EEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCHHHH
TDLAKAKQLLAEAGYPDGLETTLSFDLGFAGVNEPLCVLLQENLAQIGIKTTINKIPGAN
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
WRTELTKKVLPLFTNVFSGWLDYPEYFFFWCYHGNNSIFNTMSYQSAAMDAFIDGARAAA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHCCHHHHC
ANGDKAAYDADVKGMVDLAFADVPRIPLYQPYVNVAMQKNITGYEYWFHRRLDYRAFQKG
CCCCCCEECCCCCCCEEEHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA