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The map label for this gene is kefBC [H]

Identifier: 154248569

GI number: 154248569

Start: 5139175

End: 5141019

Strand: Direct

Name: kefBC [H]

Synonym: Xaut_4650

Alternate gene names: 154248569

Gene position: 5139175-5141019 (Clockwise)

Preceding gene: 154248568

Following gene: 154248570

Centisome position: 96.8

GC content: 69.11

Gene sequence:

>1845_bases
ATGCACGGAGATGAACACGCACTTTTCGGCGCGGCGCTCGCATTCCTAGCCGCAGCGGTCGTCTCTGTTCCCATCGCCAA
GCGCCTCGGCTTTTCCCCGGTCGTCGGTTATCTGCTGGTGGGCATCGCCATCGGCCCGGTGGGCTTCAACGTGGCCGATC
CGGGGGTGGTCTCCACCGTCGCCGAGCTCGGCGTGGTCATGCTGCTGTTCCTGGTGGGGCTGGAGCTGAAGCCGTCCAGC
CTGCTGTCCATGGGCCATTTCATCTTCGGACTGGGCACCGCGCAGCTCATGCTGACGGCGCTGGCGTTCGCGCTGCTGGC
CTATTTCGGCCTCGGCTTCGGCGCGGCGGCGGCGGTGGTGAGCGGCCTCGCCCTGTCGATCTCGGGCACCGCCATCGCGC
TCCAGCTGCTCGGCGAGCGCGGCGACCTTGCCTCCCCCTATGGCCAGCGCACCTTCTCCATCCTGCTGTTCCAGGACATC
GCGGTGGTGCCGCTGCTCGCCCTGGTGCCCCTGCTGGCGCCGGGCGGCGAGGCCGAGGTAGCGCGCGATCCCAAGGCGAC
CCTGCTCGCCGCCGCCATCGCGGTGGCAGCCATCGCCGTCATCGTGGTGTCGGGGCGCTATTTGCTCAATCCGCTGTTCC
GCGTGCTGGCCCGCTCCGGCGCGCGCGAGGCCATGACCGCCGCCGCCCTGCTGGTGGTGATGGGAGCCGCCGGCCTCATG
GAGGCGGCGGGCCTTTCGGCGGCGCTCGGGGCCTTCCTCGCCGGCCTGCTGCTGTCGGAGAGCACCTTCCGCCACGAGCT
GGAAGCCAATGTGGACGCCTTCCGCGGCCTGCTGCTCGCCGTGTTCTTCATGAGCATCGGCATGTCGCTCAACATCACCG
TGGTGCTGGGCCATATCCCGAGCCTGCTGGTGGGGACGCTGGCGGTGGCGGTGGTCAAGTCGGTGATCGTCTATCTGGTG
TTCCGGGCCGACAACATCGCTCCCCAGGATTCCATGCGCGCCGCGGTAGCGCTCATGTCCGCCGGCGAATTCGCCTTCGT
GCTGTTCCCGCTGGGCCTCGACAACAGGATCCTGAAATCCGAGCAGGCCGACCTTTTGATCGCCCTGGCCGCCCTCACCA
TGATCCTCGGCCCGCTGCTGTTCGCCGCCCTGTCGCGGCTGCTGCCGCGCTTCGCCCGACCGGCGGCCGAGCCGCCGGCG
GATGATTTCGCCGATGCGGACGGCTCGGTGCTGGTGATCGGCTTCGGCCGATTCGGCCAGATCGTCTCCCAGTGCCTGCT
GGCCCAGGAGGTGACCGTCACCATCATCGACAAGGACGTGGAGATGATCCAGTCCGCCGGGCGCTTCGGCGTGCACATCT
ATTATGGCGACGGCACCCGGCTCGACGTGCTGCGGGCCGCCGGCGCCGAGACGGTGGAAGTCATCTGCGTGTGCGTGGAC
AACAAGGAGGACGCCAACCGCATCGTCGAGATCGTGCGCGCCTCCATTCCCAACGCGCGGCTGTTCGTGCGCAGCTTCGA
CCGGGTGCATTCCATGGAGCTGGCGCGCCTTGGCGTGGATTTCGAGCTGCGCGAGACCCTCGAATCGGCCCTGTCCTTCG
GCGCCCGCACCCTGGAGGCGCTGGGCGTGCCGCAGAACGCCGCTGCCGCCCGCATCGCCGACGTGCGCGTGCGCGACCGC
GCCCGCCTGGAGCGCCAGCTGGAGGAAGGCATTTTCGCGGGTGGCGAGGTGTTCAGCGGCCACAAGGTGGAGCCCGAGCC
CCTGACCCAGCCCGAGAAGCCGGCCCGCCCGCTGAACCCGGAGGCGCAGGACATCCTGTCCCACGAAACCGAATTCTCCG
GCTGA

Upstream 100 bases:

>100_bases
GTTTGGACATGCTTGCTGCGCACAAAGCTGGAGCGGAGCGGAGTAGCGGCGGCCGGGCGGCGCCGGGGCGGTGGCTTCGG
GGACCGGCAGGGAGAGCTGG

Downstream 100 bases:

>100_bases
GCCTTGGCCGTCCGGACGCTTCTGCGGCCGGGCCGGCAGAGTCTTTGGATAGCCCCGAGGCTGTTGCAACCATTTCGTGA
TTTCGCTGCGACGATGGCGT

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MHGDEHALFGAALAFLAAAVVSVPIAKRLGFSPVVGYLLVGIAIGPVGFNVADPGVVSTVAELGVVMLLFLVGLELKPSS
LLSMGHFIFGLGTAQLMLTALAFALLAYFGLGFGAAAAVVSGLALSISGTAIALQLLGERGDLASPYGQRTFSILLFQDI
AVVPLLALVPLLAPGGEAEVARDPKATLLAAAIAVAAIAVIVVSGRYLLNPLFRVLARSGAREAMTAAALLVVMGAAGLM
EAAGLSAALGAFLAGLLLSESTFRHELEANVDAFRGLLLAVFFMSIGMSLNITVVLGHIPSLLVGTLAVAVVKSVIVYLV
FRADNIAPQDSMRAAVALMSAGEFAFVLFPLGLDNRILKSEQADLLIALAALTMILGPLLFAALSRLLPRFARPAAEPPA
DDFADADGSVLVIGFGRFGQIVSQCLLAQEVTVTIIDKDVEMIQSAGRFGVHIYYGDGTRLDVLRAAGAETVEVICVCVD
NKEDANRIVEIVRASIPNARLFVRSFDRVHSMELARLGVDFELRETLESALSFGARTLEALGVPQNAAAARIADVRVRDR
ARLERQLEEGIFAGGEVFSGHKVEPEPLTQPEKPARPLNPEAQDILSHETEFSG

Sequences:

>Translated_614_residues
MHGDEHALFGAALAFLAAAVVSVPIAKRLGFSPVVGYLLVGIAIGPVGFNVADPGVVSTVAELGVVMLLFLVGLELKPSS
LLSMGHFIFGLGTAQLMLTALAFALLAYFGLGFGAAAAVVSGLALSISGTAIALQLLGERGDLASPYGQRTFSILLFQDI
AVVPLLALVPLLAPGGEAEVARDPKATLLAAAIAVAAIAVIVVSGRYLLNPLFRVLARSGAREAMTAAALLVVMGAAGLM
EAAGLSAALGAFLAGLLLSESTFRHELEANVDAFRGLLLAVFFMSIGMSLNITVVLGHIPSLLVGTLAVAVVKSVIVYLV
FRADNIAPQDSMRAAVALMSAGEFAFVLFPLGLDNRILKSEQADLLIALAALTMILGPLLFAALSRLLPRFARPAAEPPA
DDFADADGSVLVIGFGRFGQIVSQCLLAQEVTVTIIDKDVEMIQSAGRFGVHIYYGDGTRLDVLRAAGAETVEVICVCVD
NKEDANRIVEIVRASIPNARLFVRSFDRVHSMELARLGVDFELRETLESALSFGARTLEALGVPQNAAAARIADVRVRDR
ARLERQLEEGIFAGGEVFSGHKVEPEPLTQPEKPARPLNPEAQDILSHETEFSG
>Mature_614_residues
MHGDEHALFGAALAFLAAAVVSVPIAKRLGFSPVVGYLLVGIAIGPVGFNVADPGVVSTVAELGVVMLLFLVGLELKPSS
LLSMGHFIFGLGTAQLMLTALAFALLAYFGLGFGAAAAVVSGLALSISGTAIALQLLGERGDLASPYGQRTFSILLFQDI
AVVPLLALVPLLAPGGEAEVARDPKATLLAAAIAVAAIAVIVVSGRYLLNPLFRVLARSGAREAMTAAALLVVMGAAGLM
EAAGLSAALGAFLAGLLLSESTFRHELEANVDAFRGLLLAVFFMSIGMSLNITVVLGHIPSLLVGTLAVAVVKSVIVYLV
FRADNIAPQDSMRAAVALMSAGEFAFVLFPLGLDNRILKSEQADLLIALAALTMILGPLLFAALSRLLPRFARPAAEPPA
DDFADADGSVLVIGFGRFGQIVSQCLLAQEVTVTIIDKDVEMIQSAGRFGVHIYYGDGTRLDVLRAAGAETVEVICVCVD
NKEDANRIVEIVRASIPNARLFVRSFDRVHSMELARLGVDFELRETLESALSFGARTLEALGVPQNAAAARIADVRVRDR
ARLERQLEEGIFAGGEVFSGHKVEPEPLTQPEKPARPLNPEAQDILSHETEFSG

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=389, Percent_Identity=28.2776349614396, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1789749, Length=563, Percent_Identity=42.4511545293073, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1786232, Length=556, Percent_Identity=37.410071942446, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1786685, Length=499, Percent_Identity=30.2605210420842, Blast_Score=163, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 64431; Mature: 64431

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGDEHALFGAALAFLAAAVVSVPIAKRLGFSPVVGYLLVGIAIGPVGFNVADPGVVSTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AELGVVMLLFLVGLELKPSSLLSMGHFIFGLGTAQLMLTALAFALLAYFGLGFGAAAAVV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SGLALSISGTAIALQLLGERGDLASPYGQRTFSILLFQDIAVVPLLALVPLLAPGGEAEV
HHHHEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ARDPKATLLAAAIAVAAIAVIVVSGRYLLNPLFRVLARSGAREAMTAAALLVVMGAAGLM
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EAAGLSAALGAFLAGLLLSESTFRHELEANVDAFRGLLLAVFFMSIGMSLNITVVLGHIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
SLLVGTLAVAVVKSVIVYLVFRADNIAPQDSMRAAVALMSAGEFAFVLFPLGLDNRILKS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
EQADLLIALAALTMILGPLLFAALSRLLPRFARPAAEPPADDFADADGSVLVIGFGRFGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEEEEECCHHHH
IVSQCLLAQEVTVTIIDKDVEMIQSAGRFGVHIYYGDGTRLDVLRAAGAETVEVICVCVD
HHHHHHHHHCCEEEEECHHHHHHHCCCCCEEEEEECCCCEEHHHHHCCCCEEEEEEEEEC
NKEDANRIVEIVRASIPNARLFVRSFDRVHSMELARLGVDFELRETLESALSFGARTLEA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LGVPQNAAAARIADVRVRDRARLERQLEEGIFAGGEVFSGHKVEPEPLTQPEKPARPLNP
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EAQDILSHETEFSG
CHHHHHHCCCCCCC
>Mature Secondary Structure
MHGDEHALFGAALAFLAAAVVSVPIAKRLGFSPVVGYLLVGIAIGPVGFNVADPGVVSTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AELGVVMLLFLVGLELKPSSLLSMGHFIFGLGTAQLMLTALAFALLAYFGLGFGAAAAVV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SGLALSISGTAIALQLLGERGDLASPYGQRTFSILLFQDIAVVPLLALVPLLAPGGEAEV
HHHHEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ARDPKATLLAAAIAVAAIAVIVVSGRYLLNPLFRVLARSGAREAMTAAALLVVMGAAGLM
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EAAGLSAALGAFLAGLLLSESTFRHELEANVDAFRGLLLAVFFMSIGMSLNITVVLGHIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
SLLVGTLAVAVVKSVIVYLVFRADNIAPQDSMRAAVALMSAGEFAFVLFPLGLDNRILKS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
EQADLLIALAALTMILGPLLFAALSRLLPRFARPAAEPPADDFADADGSVLVIGFGRFGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEEEEECCHHHH
IVSQCLLAQEVTVTIIDKDVEMIQSAGRFGVHIYYGDGTRLDVLRAAGAETVEVICVCVD
HHHHHHHHHCCEEEEECHHHHHHHCCCCCEEEEEECCCCEEHHHHHCCCCEEEEEEEEEC
NKEDANRIVEIVRASIPNARLFVRSFDRVHSMELARLGVDFELRETLESALSFGARTLEA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LGVPQNAAAARIADVRVRDRARLERQLEEGIFAGGEVFSGHKVEPEPLTQPEKPARPLNP
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EAQDILSHETEFSG
CHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]