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The map label for this gene is nuoB

Identifier: 154248551

GI number: 154248551

Start: 5119506

End: 5120090

Strand: Reverse

Name: nuoB

Synonym: Xaut_4632

Alternate gene names: 154248551

Gene position: 5120090-5119506 (Counterclockwise)

Preceding gene: 154248552

Following gene: 154248550

Centisome position: 96.44

GC content: 66.5

Gene sequence:

>585_bases
ATGGGACTGACCCCTTCGGCCACCAAGCCCGAGATCGCACAGGCCCCCCAGGGCATCGTCGATCCCTCCACCGGCCGCCC
CGTGGGCGCGGATGATCCCTTCTTTAAGGAGATCAATTCCGAGCTGGCGGACAAGGGCTTCATCCTGACCACCGCCGACG
ACCTCATCACCTGGGCGCGCACCGGCTCGCTCATGTGGATGACCTTCGGCCTCGCCTGCTGCGCCGTGGAGATGATGCAG
GTCTCCATGCCACGCTACGACGTGGAGCGCTTCGGTTTCGCGCCGCGCGCCTCGCCGCGCCAGTCGGACGTGATGATCGT
CGCCGGCACGCTGACCAACAAGATGGCCCCGGCCCTGCGCAAGGTCTACGACCAGATGCCGGAGCCGCGCTACGTCATCT
CCATGGGCTCCTGCGCCAATGGCGGCGGCTACTACCACTTCTCCTATGCGGTGGTGCGCGGCTGTGATCGCATCGTGCCG
GTGGACATCTACGTGCCGGGCTGCCCGCCCACTGCGGAAGCGCTGCTGTATGGCGTGCTGCTGCTGCAGAAGAAGATCCG
CCGCATCGGCACCATCGAGCGCTGA

Upstream 100 bases:

>100_bases
TGTTCGGTGGCCTCGGCGACCTCGGCTTCTGGTCGATGATGACCTTCCTCGGTGTCCTCACCGTTGGCTTCATCTACGAA
TGGCGCAAGGGAGCTCTGGA

Downstream 100 bases:

>100_bases
GCCGGGAGCATTTCGATGGACGAGACCCTCAACGACCTCGCGGCGCACGTATCGGCGGCCCTTTCGGGCGCCGTGCTCGG
CTCTCTCGTGGCCCATGGCG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MGLTPSATKPEIAQAPQGIVDPSTGRPVGADDPFFKEINSELADKGFILTTADDLITWARTGSLMWMTFGLACCAVEMMQ
VSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHFSYAVVRGCDRIVP
VDIYVPGCPPTAEALLYGVLLLQKKIRRIGTIER

Sequences:

>Translated_194_residues
MGLTPSATKPEIAQAPQGIVDPSTGRPVGADDPFFKEINSELADKGFILTTADDLITWARTGSLMWMTFGLACCAVEMMQ
VSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHFSYAVVRGCDRIVP
VDIYVPGCPPTAEALLYGVLLLQKKIRRIGTIER
>Mature_193_residues
GLTPSATKPEIAQAPQGIVDPSTGRPVGADDPFFKEINSELADKGFILTTADDLITWARTGSLMWMTFGLACCAVEMMQV
SMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHFSYAVVRGCDRIVPV
DIYVPGCPPTAEALLYGVLLLQKKIRRIGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=142, Percent_Identity=79.5774647887324, Blast_Score=248, Evalue=3e-66,
Organism=Escherichia coli, GI1788624, Length=156, Percent_Identity=48.0769230769231, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=43.6507936507937, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788834, Length=125, Percent_Identity=40, Blast_Score=100, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=176, Percent_Identity=61.3636363636364, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI18859983, Length=159, Percent_Identity=71.0691823899371, Blast_Score=247, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24642371, Length=159, Percent_Identity=71.0691823899371, Blast_Score=247, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=76.5957446808511, Blast_Score=241, Evalue=3e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_XANP2 (A7IPA6)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419509.1
- ProteinModelPortal:   A7IPA6
- SMR:   A7IPA6
- STRING:   A7IPA6
- GeneID:   5424064
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4632
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   HYDNERF
- ProtClustDB:   PRK06411
- BioCyc:   XAUT78245:XAUT_4632-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 21232; Mature: 21101

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTPSATKPEIAQAPQGIVDPSTGRPVGADDPFFKEINSELADKGFILTTADDLITWAR
CCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHH
TGSLMWMTFGLACCAVEMMQVSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALR
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHH
KVYDQMPEPRYVISMGSCANGGGYYHFSYAVVRGCDRIVPVDIYVPGCPPTAEALLYGVL
HHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHH
LLQKKIRRIGTIER
HHHHHHHHHCCCCC
>Mature Secondary Structure 
GLTPSATKPEIAQAPQGIVDPSTGRPVGADDPFFKEINSELADKGFILTTADDLITWAR
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHH
TGSLMWMTFGLACCAVEMMQVSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALR
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHH
KVYDQMPEPRYVISMGSCANGGGYYHFSYAVVRGCDRIVPVDIYVPGCPPTAEALLYGVL
HHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHH
LLQKKIRRIGTIER
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA