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The map label for this gene is trmJ [H]

Identifier: 154248490

GI number: 154248490

Start: 5051633

End: 5052529

Strand: Direct

Name: trmJ [H]

Synonym: Xaut_4570

Alternate gene names: 154248490

Gene position: 5051633-5052529 (Clockwise)

Preceding gene: 154248488

Following gene: 154248493

Centisome position: 95.15

GC content: 72.35

Gene sequence:

>897_bases
ATGCCGGGTGCCGGGACCGACAGCACGAAACCATGGGTGGCCGGCGGGCCGGTGGTGATCCTGGTGGAGCCGCAGCTGGG
CCAGAATATCGGCTCGGCGGCGCGGGCCATGGGCAATTTCGGCCTCTCGCGCCTGCGCATCGTGAACCCCCGCGAGGGCT
GGCCCAGCGAGCCGGCCCGCATCTTCTCGGCCGGGGCCGACCGCATCCTCGCCGAGGCCGTGCTCTATCCGGACCTGCGC
TCGGCCCTCGTGGGCGTCAACTATGCCTTCGCCGCCACCGCCCGCGAGCGCGGCATGGCCAAGCCCGTGCTCGGCGCCGA
CGCGGTGGCGCAGGAGGCCCGCTCCCGCCTTGCCGGCGGGGAAGACGTGGCCCTGGTGTTCGGCCGCGAGCGCACCGGCC
TCTATACGGAGGAGGTGTCGCTGTGCGACGCCATCCTCACCCTGCCGGTGAACCCCGCCTTCGCCTCGCTGAACCTCGCC
ATGTGCGTGGCGGTGACCGGCTATGAATGGTTCAAGGCGGAAACCGGGGGCGCCCTGCCCTTCGCCCCGCCGGACCGCTC
GCCGCTGGCGGACAAGGGCGACCTCCTCGCCTTCTTCGATCACCTGGAAGGGGCGCTGGAGCAGTCGGGCTTCTTCCGCT
CGCCGGAAAAGGCGCCCTCCACCATCCGCAACCTGCGCAACATCTTCCATCGCCTCGGCCTGACGCGGCAGGACCTCGCC
ACGCTGCACGGCGCCGTCACCGCGCTGGAGGAAGGCCGAGCCGGCCGCGACGCGCGCAAGACCATCCGTATCGCCGACGC
CGCCATCCACAAGAGCCGCAAGGCGACGGCACGCAAGACGCCGCCGGAAAGCGCGGAGCCGACGGCGCCGGTACCGCCCG
AGGGCGAGGGCGACTGA

Upstream 100 bases:

>100_bases
CGGGCTTGACCACTGGCCCATGAGCCAAGCGCATCGGCATGACATCACCCCTCGACTTGGCTGCCCACCCTGTGCAAAAC
AGCGCGAAACGGGAGACGAC

Downstream 100 bases:

>100_bases
GCTTCAGCCGCCGAGCCTTTCGCCTAGAAAAGCCCCGGCCTCGGCCAGCGCCCGGCGCCCGTCGGCGAGGCGGTGGTACC
AGATGGGCCAGGCGTGGATC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MPGAGTDSTKPWVAGGPVVILVEPQLGQNIGSAARAMGNFGLSRLRIVNPREGWPSEPARIFSAGADRILAEAVLYPDLR
SALVGVNYAFAATARERGMAKPVLGADAVAQEARSRLAGGEDVALVFGRERTGLYTEEVSLCDAILTLPVNPAFASLNLA
MCVAVTGYEWFKAETGGALPFAPPDRSPLADKGDLLAFFDHLEGALEQSGFFRSPEKAPSTIRNLRNIFHRLGLTRQDLA
TLHGAVTALEEGRAGRDARKTIRIADAAIHKSRKATARKTPPESAEPTAPVPPEGEGD

Sequences:

>Translated_298_residues
MPGAGTDSTKPWVAGGPVVILVEPQLGQNIGSAARAMGNFGLSRLRIVNPREGWPSEPARIFSAGADRILAEAVLYPDLR
SALVGVNYAFAATARERGMAKPVLGADAVAQEARSRLAGGEDVALVFGRERTGLYTEEVSLCDAILTLPVNPAFASLNLA
MCVAVTGYEWFKAETGGALPFAPPDRSPLADKGDLLAFFDHLEGALEQSGFFRSPEKAPSTIRNLRNIFHRLGLTRQDLA
TLHGAVTALEEGRAGRDARKTIRIADAAIHKSRKATARKTPPESAEPTAPVPPEGEGD
>Mature_297_residues
PGAGTDSTKPWVAGGPVVILVEPQLGQNIGSAARAMGNFGLSRLRIVNPREGWPSEPARIFSAGADRILAEAVLYPDLRS
ALVGVNYAFAATARERGMAKPVLGADAVAQEARSRLAGGEDVALVFGRERTGLYTEEVSLCDAILTLPVNPAFASLNLAM
CVAVTGYEWFKAETGGALPFAPPDRSPLADKGDLLAFFDHLEGALEQSGFFRSPEKAPSTIRNLRNIFHRLGLTRQDLAT
LHGAVTALEEGRAGRDARKTIRIADAAIHKSRKATARKTPPESAEPTAPVPPEGEGD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=250, Percent_Identity=36, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790865, Length=240, Percent_Identity=32.0833333333333, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31602; Mature: 31471

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGAGTDSTKPWVAGGPVVILVEPQLGQNIGSAARAMGNFGLSRLRIVNPREGWPSEPAR
CCCCCCCCCCCEECCCCEEEEECCHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
IFSAGADRILAEAVLYPDLRSALVGVNYAFAATARERGMAKPVLGADAVAQEARSRLAGG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
EDVALVFGRERTGLYTEEVSLCDAILTLPVNPAFASLNLAMCVAVTGYEWFKAETGGALP
CCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHEEHEEECCCHHEECCCCCCCC
FAPPDRSPLADKGDLLAFFDHLEGALEQSGFFRSPEKAPSTIRNLRNIFHRLGLTRQDLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
TLHGAVTALEEGRAGRDARKTIRIADAAIHKSRKATARKTPPESAEPTAPVPPEGEGD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PGAGTDSTKPWVAGGPVVILVEPQLGQNIGSAARAMGNFGLSRLRIVNPREGWPSEPAR
CCCCCCCCCCEECCCCEEEEECCHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
IFSAGADRILAEAVLYPDLRSALVGVNYAFAATARERGMAKPVLGADAVAQEARSRLAGG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
EDVALVFGRERTGLYTEEVSLCDAILTLPVNPAFASLNLAMCVAVTGYEWFKAETGGALP
CCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHEEHEEECCCHHEECCCCCCCC
FAPPDRSPLADKGDLLAFFDHLEGALEQSGFFRSPEKAPSTIRNLRNIFHRLGLTRQDLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
TLHGAVTALEEGRAGRDARKTIRIADAAIHKSRKATARKTPPESAEPTAPVPPEGEGD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA