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The map label for this gene is ccsA [H]

Identifier: 154248345

GI number: 154248345

Start: 4909314

End: 4910624

Strand: Direct

Name: ccsA [H]

Synonym: Xaut_4425

Alternate gene names: 154248345

Gene position: 4909314-4910624 (Clockwise)

Preceding gene: 154248344

Following gene: 154248362

Centisome position: 92.47

GC content: 62.32

Gene sequence:

>1311_bases
ATGGACGCCACCACCAATACCGGGGCAAAAGCGGCCAAGCTGAGCTTGGGGGAGAAGAGCTGGGACCTGCCGATCCACGG
AGGGACCATCGGCCCCGACGTGGTCGATATCTCGAAGCTCTACGGTCAGACCGGGCTGTTCACCTACGACCCCGGCTTTA
CCTCCACCGCTTCGTGTGAAAGCAAGATCACCTATATCGACGGTGATGAGGGCGTGCTGCTCTATCGCGGCTACCCCATT
GAGCAGCTGGCCGAGAACGGCGACTTCCTCGAGACCTGCTATCTCCTGCTCTACGGCGAATTGCCGACCGCCGCCCAGAA
GGCCGAGTTCGACCTCTCCGTGACCCGTCACACGATGGTTCACGAGCAGATGACGCGCTTCTTCCAGGGATTCCGCCGCG
ACGCCCACCCCATGGCGATCATGGTGGCATCCGTCGGCGCGCTCTCCGCCTTCTATCACGACTCCACTGATATTTCTGAC
CCCACCCAGCGCCTGATCGCGTCGATTCGCATGATTGCGAAGATGCCGACGCTGGCGGCCATGGCCTACAAGTATTCCAT
CGGCCAGCCGTTCGTTTACCCCAAGAACGAACTCGACTATACGTCGAACTTCCTGAACATGTGCTTCGCCGTTCCGTGCG
AGGATTACAAGGTCAACCCCATCCTCGCGAAGGCGATGGACAAGATCTTCATCCTCCACGCCGATCACGAGCAGAACGCC
TCCACCTCCACGGTGCGCCTCGCCGGCTCCTCCGGCGCCAATCCGTTTGCCTGCATCGCCGCCGGCATCGCCTGCCTGTG
GGGACCCGCCCATGGCGGCGCCAACGAGGCCGCCCTCAAGATGCTCACCGAGATCGGCCATGTGGACCGCATCCCGGAGT
ACATCAAGCGGTCAAAGGACAAGAACGACCCCTTCCGCCTCATGGGCTTCGGCCACCGGGTCTACAAGAACTACGACCCG
CGCGCCAAGATCATGCAGCAGACCTGCCATGAAGTGCTCGGCGAGCTGGGCATCAAGGACGATCCGCTTCTGGACGTGGC
CGTCGAGCTGGAGCGCATCGCGCTCTCCGACGACTATTTCATCGAGAAGAAGCTCTATCCGAACATCGACTTCTACTCGG
GCATCACCCTGAAGGCGATGGGCTTCCCCACCACCATGTTCACCGTGCTGTTCGCCCTGGCGCGCACCGTGGGCTGGATC
GGCCAGTGGAAGGAGATGATCGAGGATCCGGGCCAGCGCATCGGCCGTCCCCGCCAGCTCTACACCGGCGCCGCCCTGCG
CGACTACGTCGCCATGTCCAAGCGCAAGTGA

Upstream 100 bases:

>100_bases
ACGCCAGAGCGGGGACGGACTTGCCACTTGAGGAACCGGATCGCCACCTCCGGATCCGCGTGGAGATCCGGTTCTTGTCC
GCCCAAAGGATGAGGGATCC

Downstream 100 bases:

>100_bases
TCCAAAGGCCGACCTTCAAGGCCGGTTGAAATGTGGAAAGCGCGGCCTCAGGGCCGCGCTTTTCGTTTGTGTGCAGGACA
TTGGGCTGCGCCAAGACATT

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MDATTNTGAKAAKLSLGEKSWDLPIHGGTIGPDVVDISKLYGQTGLFTYDPGFTSTASCESKITYIDGDEGVLLYRGYPI
EQLAENGDFLETCYLLLYGELPTAAQKAEFDLSVTRHTMVHEQMTRFFQGFRRDAHPMAIMVASVGALSAFYHDSTDISD
PTQRLIASIRMIAKMPTLAAMAYKYSIGQPFVYPKNELDYTSNFLNMCFAVPCEDYKVNPILAKAMDKIFILHADHEQNA
STSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALKMLTEIGHVDRIPEYIKRSKDKNDPFRLMGFGHRVYKNYDP
RAKIMQQTCHEVLGELGIKDDPLLDVAVELERIALSDDYFIEKKLYPNIDFYSGITLKAMGFPTTMFTVLFALARTVGWI
GQWKEMIEDPGQRIGRPRQLYTGAALRDYVAMSKRK

Sequences:

>Translated_436_residues
MDATTNTGAKAAKLSLGEKSWDLPIHGGTIGPDVVDISKLYGQTGLFTYDPGFTSTASCESKITYIDGDEGVLLYRGYPI
EQLAENGDFLETCYLLLYGELPTAAQKAEFDLSVTRHTMVHEQMTRFFQGFRRDAHPMAIMVASVGALSAFYHDSTDISD
PTQRLIASIRMIAKMPTLAAMAYKYSIGQPFVYPKNELDYTSNFLNMCFAVPCEDYKVNPILAKAMDKIFILHADHEQNA
STSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALKMLTEIGHVDRIPEYIKRSKDKNDPFRLMGFGHRVYKNYDP
RAKIMQQTCHEVLGELGIKDDPLLDVAVELERIALSDDYFIEKKLYPNIDFYSGITLKAMGFPTTMFTVLFALARTVGWI
GQWKEMIEDPGQRIGRPRQLYTGAALRDYVAMSKRK
>Mature_436_residues
MDATTNTGAKAAKLSLGEKSWDLPIHGGTIGPDVVDISKLYGQTGLFTYDPGFTSTASCESKITYIDGDEGVLLYRGYPI
EQLAENGDFLETCYLLLYGELPTAAQKAEFDLSVTRHTMVHEQMTRFFQGFRRDAHPMAIMVASVGALSAFYHDSTDISD
PTQRLIASIRMIAKMPTLAAMAYKYSIGQPFVYPKNELDYTSNFLNMCFAVPCEDYKVNPILAKAMDKIFILHADHEQNA
STSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALKMLTEIGHVDRIPEYIKRSKDKNDPFRLMGFGHRVYKNYDP
RAKIMQQTCHEVLGELGIKDDPLLDVAVELERIALSDDYFIEKKLYPNIDFYSGITLKAMGFPTTMFTVLFALARTVGWI
GQWKEMIEDPGQRIGRPRQLYTGAALRDYVAMSKRK

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=441, Percent_Identity=25.6235827664399, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786939, Length=425, Percent_Identity=69.4117647058823, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1786527, Length=366, Percent_Identity=29.7814207650273, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17555174, Length=446, Percent_Identity=26.0089686098655, Blast_Score=118, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6319850, Length=350, Percent_Identity=26, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324328, Length=352, Percent_Identity=26.1363636363636, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325257, Length=402, Percent_Identity=27.1144278606965, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640126, Length=434, Percent_Identity=26.4976958525346, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24640124, Length=434, Percent_Identity=26.4976958525346, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21356863, Length=420, Percent_Identity=25.952380952381, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48480; Mature: 48480

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDATTNTGAKAAKLSLGEKSWDLPIHGGTIGPDVVDISKLYGQTGLFTYDPGFTSTASCE
CCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCC
SKITYIDGDEGVLLYRGYPIEQLAENGDFLETCYLLLYGELPTAAQKAEFDLSVTRHTMV
CEEEEEECCCCEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHH
HEQMTRFFQGFRRDAHPMAIMVASVGALSAFYHDSTDISDPTQRLIASIRMIAKMPTLAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHH
MAYKYSIGQPFVYPKNELDYTSNFLNMCFAVPCEDYKVNPILAKAMDKIFILHADHEQNA
HHHHHCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHCEEEEEECCCCCCC
STSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALKMLTEIGHVDRIPEYIKRSKD
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
KNDPFRLMGFGHRVYKNYDPRAKIMQQTCHEVLGELGIKDDPLLDVAVELERIALSDDYF
CCCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCE
IEKKLYPNIDFYSGITLKAMGFPTTMFTVLFALARTVGWIGQWKEMIEDPGQRIGRPRQL
EEHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHH
YTGAALRDYVAMSKRK
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDATTNTGAKAAKLSLGEKSWDLPIHGGTIGPDVVDISKLYGQTGLFTYDPGFTSTASCE
CCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCC
SKITYIDGDEGVLLYRGYPIEQLAENGDFLETCYLLLYGELPTAAQKAEFDLSVTRHTMV
CEEEEEECCCCEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHH
HEQMTRFFQGFRRDAHPMAIMVASVGALSAFYHDSTDISDPTQRLIASIRMIAKMPTLAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHH
MAYKYSIGQPFVYPKNELDYTSNFLNMCFAVPCEDYKVNPILAKAMDKIFILHADHEQNA
HHHHHCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHCEEEEEECCCCCCC
STSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALKMLTEIGHVDRIPEYIKRSKD
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
KNDPFRLMGFGHRVYKNYDPRAKIMQQTCHEVLGELGIKDDPLLDVAVELERIALSDDYF
CCCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCE
IEKKLYPNIDFYSGITLKAMGFPTTMFTVLFALARTVGWIGQWKEMIEDPGQRIGRPRQL
EEHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHH
YTGAALRDYVAMSKRK
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]