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The map label for this gene is dat [H]

Identifier: 154248314

GI number: 154248314

Start: 4870710

End: 4871567

Strand: Reverse

Name: dat [H]

Synonym: Xaut_4394

Alternate gene names: 154248314

Gene position: 4871567-4870710 (Counterclockwise)

Preceding gene: 154248315

Following gene: 154248313

Centisome position: 91.76

GC content: 67.48

Gene sequence:

>858_bases
ATGTCCCGCATCGCCTATGTCAATGGCCGCTACCTGCCGCACCGCCATGCCAGCGTGCATGTGGAGGATCGCGGCTATCA
GTTCGCCGATGGCGTCTATGAAGTCTGCGAAGTGCTCAACGGCCATCTGGTGGACGAGCGGCGCCACCTCGACCGGCTGG
AGCGTTCGCTGCGGGAGCTGTTCATCCCCACGCCCATGAGCCGGATCGCCCTCGCCAACGTGCTGGCGGAGGTGGTGCGG
CGGAACGGGGTGCGCGACGGGCTGGTCTATCTCCAGATCACCCGCGGTGTGGCCCGGCGCGACCACGCCTTCCCGCCGGC
CGGCACCAAGCCTGCGGTGGTGGTGACGGCGCGCCGGTCCGACCGCAAGGGGCAGGAGGCGCTGGCCGCCAAGGGCGTCG
CCGTCATCACCGTGCCGGAGAACCGCTGGCCGCGCGTGGACATCAAGTCCACCTCGCTGTTGCCCAATGTGCTGGCCAAG
CAGCAGGCGAAAGAGGCTGGCGCACGGGAAGCCTGGTTCGTGGACGGCGCGGGCTTCGTCACCGAGGGTGCCTCCACCAA
CGCCTGGATCGTGACGGGCGGCAAGACCATCGTCACCCGCCCGGCGGAGAGTGGTATCCTGCGCGGAATCACCCGCACGG
TGGTGTTTGAGGTGGCGGCCGCCCTCGGCTACACGGTGGAGGAGCGTCCCTTTACCGTTGCGGAAGCTTTGGCCGCGGAC
GAAGCCTTCATCACCGCCGCCACCACGGTGGTGATGCCTGTGGTGCGCATCGACGGCCATGCGGTGGGGGACGGCAAGCC
CGGCCCCGTGGCGACCACTTTACGCTTGAAATTCCACGATTTTTCCGAAATTTTCTGA

Upstream 100 bases:

>100_bases
GGTGGCCGGGCTCGGCATGGTGGCGGGGCGGCTGGAGATGATCGGCCTCCTCGTCGTCATCCACCTTGCTCTCTGGCGCA
CCTGAAGAAGCGTTTTTGCC

Downstream 100 bases:

>100_bases
GGTATCCACGGCCGCGCTCATGTTGCGGGGCAAAATAATATTGCGCCGCCATGGAGCTGTGCCATTCTAGGAGGCCAACT
CCCGTGGCGGCAGCACATTA

Product: class IV aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSRIAYVNGRYLPHRHASVHVEDRGYQFADGVYEVCEVLNGHLVDERRHLDRLERSLRELFIPTPMSRIALANVLAEVVR
RNGVRDGLVYLQITRGVARRDHAFPPAGTKPAVVVTARRSDRKGQEALAAKGVAVITVPENRWPRVDIKSTSLLPNVLAK
QQAKEAGAREAWFVDGAGFVTEGASTNAWIVTGGKTIVTRPAESGILRGITRTVVFEVAAALGYTVEERPFTVAEALAAD
EAFITAATTVVMPVVRIDGHAVGDGKPGPVATTLRLKFHDFSEIF

Sequences:

>Translated_285_residues
MSRIAYVNGRYLPHRHASVHVEDRGYQFADGVYEVCEVLNGHLVDERRHLDRLERSLRELFIPTPMSRIALANVLAEVVR
RNGVRDGLVYLQITRGVARRDHAFPPAGTKPAVVVTARRSDRKGQEALAAKGVAVITVPENRWPRVDIKSTSLLPNVLAK
QQAKEAGAREAWFVDGAGFVTEGASTNAWIVTGGKTIVTRPAESGILRGITRTVVFEVAAALGYTVEERPFTVAEALAAD
EAFITAATTVVMPVVRIDGHAVGDGKPGPVATTLRLKFHDFSEIF
>Mature_284_residues
SRIAYVNGRYLPHRHASVHVEDRGYQFADGVYEVCEVLNGHLVDERRHLDRLERSLRELFIPTPMSRIALANVLAEVVRR
NGVRDGLVYLQITRGVARRDHAFPPAGTKPAVVVTARRSDRKGQEALAAKGVAVITVPENRWPRVDIKSTSLLPNVLAKQ
QAKEAGAREAWFVDGAGFVTEGASTNAWIVTGGKTIVTRPAESGILRGITRTVVFEVAAALGYTVEERPFTVAEALAADE
AFITAATTVVMPVVRIDGHAVGDGKPGPVATTLRLKFHDFSEIF

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=283, Percent_Identity=27.208480565371, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787338, Length=264, Percent_Identity=27.6515151515151, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31111; Mature: 30980

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIAYVNGRYLPHRHASVHVEDRGYQFADGVYEVCEVLNGHLVDERRHLDRLERSLREL
CCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FIPTPMSRIALANVLAEVVRRNGVRDGLVYLQITRGVARRDHAFPPAGTKPAVVVTARRS
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCEEEEEECCC
DRKGQEALAAKGVAVITVPENRWPRVDIKSTSLLPNVLAKQQAKEAGAREAWFVDGAGFV
CCCHHHHHHHCCEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCEE
TEGASTNAWIVTGGKTIVTRPAESGILRGITRTVVFEVAAALGYTVEERPFTVAEALAAD
ECCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
EAFITAATTVVMPVVRIDGHAVGDGKPGPVATTLRLKFHDFSEIF
HHHHHHHHHHHHHHHEECCEECCCCCCCCEEEEEEEEECCHHHCC
>Mature Secondary Structure 
SRIAYVNGRYLPHRHASVHVEDRGYQFADGVYEVCEVLNGHLVDERRHLDRLERSLREL
CCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FIPTPMSRIALANVLAEVVRRNGVRDGLVYLQITRGVARRDHAFPPAGTKPAVVVTARRS
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCEEEEEECCC
DRKGQEALAAKGVAVITVPENRWPRVDIKSTSLLPNVLAKQQAKEAGAREAWFVDGAGFV
CCCHHHHHHHCCEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCEE
TEGASTNAWIVTGGKTIVTRPAESGILRGITRTVVFEVAAALGYTVEERPFTVAEALAAD
ECCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
EAFITAATTVVMPVVRIDGHAVGDGKPGPVATTLRLKFHDFSEIF
HHHHHHHHHHHHHHHEECCEECCCCCCCCEEEEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]